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Becton Dickinson luria bertani media
Luria Bertani Media, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/luria bertani media/product/Becton Dickinson
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
luria bertani media - by Bioz Stars, 2020-04
94/100 stars

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Clone Assay:

Article Title: Inhibitory effects of interleukin-10 plasmid DNA on the development of atopic dermatitis-like skin lesions in NC/Nga mice
Article Snippet: Purified mouse IL-10 gene was cloned into pGEM-T Easy vector (Promega, USA), and this gene was then sub-cloned into the Not I site of the mammalian expression vector pcDNA3.1 (Invitrogen, USA) to generate pcDNA3.1-IL-10 (named IL-10 plasmid DNA). .. The transformants were selected on Luria-Bertani media (BD Biosciences, USA) supplemented with 100 µg/mL ampicillin and the plasmid DNA was extracted using a QIAprep Spin Miniprep Kit (Qiagen, USA) according to the manufacturer's instructions.

Centrifugation:

Article Title: Applying a Linear Amplification Strategy to Recombinase Polymerase Amplification for Uniform DNA Library Amplification
Article Snippet: E. coli EcNR2 strain was incubated in Luria–Bertani media (BD Biosciences, USA) at 30 °C in a shaking incubator. .. After harvesting EcNR2 cells by centrifugation, we precipitated the genomic DNA using the GeneAll Exgene Cell SV Kit (GeneAll, Korea) according to the manufacturer’s protocol.

Amplification:

Article Title: Inhibitory effects of interleukin-10 plasmid DNA on the development of atopic dermatitis-like skin lesions in NC/Nga mice
Article Snippet: The amplified 549 bp PCR products were separated by 0.7% (w/v) agarose gel electrophoresis and purified using a QIAquick gel extraction kit (Qiagen, USA) according to the manufacturer's instructions. .. The transformants were selected on Luria-Bertani media (BD Biosciences, USA) supplemented with 100 µg/mL ampicillin and the plasmid DNA was extracted using a QIAprep Spin Miniprep Kit (Qiagen, USA) according to the manufacturer's instructions.

Agarose Gel Electrophoresis:

Article Title: Applying a Linear Amplification Strategy to Recombinase Polymerase Amplification for Uniform DNA Library Amplification
Article Snippet: E. coli EcNR2 strain was incubated in Luria–Bertani media (BD Biosciences, USA) at 30 °C in a shaking incubator. .. The size of sheared DNA was measured by agarose gel electrophoresis and an Agilent 4200 TapeStation system (Agilent, USA).

Article Title: Inhibitory effects of interleukin-10 plasmid DNA on the development of atopic dermatitis-like skin lesions in NC/Nga mice
Article Snippet: The amplified 549 bp PCR products were separated by 0.7% (w/v) agarose gel electrophoresis and purified using a QIAquick gel extraction kit (Qiagen, USA) according to the manufacturer's instructions. .. The transformants were selected on Luria-Bertani media (BD Biosciences, USA) supplemented with 100 µg/mL ampicillin and the plasmid DNA was extracted using a QIAprep Spin Miniprep Kit (Qiagen, USA) according to the manufacturer's instructions.

Expressing:

Article Title: A vaccine candidate for post-weaning diarrhea in swine constructed with a live attenuated Salmonella delivering Escherichia coli K88ab, K88ac, FedA, and FedF fimbrial antigens and its immune responses in a murine model
Article Snippet: The strain JOL912 was used for delivery and expression of K88ab, K88ac, FedA, and FedF fimbrial proteins. .. All strains were grown in Luria-Bertani media (LB; Becton, Dickinson and Company, Sparks, Maryland, USA) at 37°C.

Article Title: Inhibitory effects of interleukin-10 plasmid DNA on the development of atopic dermatitis-like skin lesions in NC/Nga mice
Article Snippet: Purified mouse IL-10 gene was cloned into pGEM-T Easy vector (Promega, USA), and this gene was then sub-cloned into the Not I site of the mammalian expression vector pcDNA3.1 (Invitrogen, USA) to generate pcDNA3.1-IL-10 (named IL-10 plasmid DNA). .. The transformants were selected on Luria-Bertani media (BD Biosciences, USA) supplemented with 100 µg/mL ampicillin and the plasmid DNA was extracted using a QIAprep Spin Miniprep Kit (Qiagen, USA) according to the manufacturer's instructions.

Synthesized:

Article Title: Inhibitory effects of interleukin-10 plasmid DNA on the development of atopic dermatitis-like skin lesions in NC/Nga mice
Article Snippet: Mouse IL-10 gene was amplified from the synthesized cDNA using polymerase chain reaction (PCR) with the following primers: forward primer, 5'-TCC ATC ATG CCT GGC TCA-3' and reverse primer, 5'-GGT GTT TTA GCT TTT CAT TTT-3' (Genebank accession number: NM_010548). .. The transformants were selected on Luria-Bertani media (BD Biosciences, USA) supplemented with 100 µg/mL ampicillin and the plasmid DNA was extracted using a QIAprep Spin Miniprep Kit (Qiagen, USA) according to the manufacturer's instructions.

Incubation:

Article Title: Applying a Linear Amplification Strategy to Recombinase Polymerase Amplification for Uniform DNA Library Amplification
Article Snippet: .. E. coli EcNR2 strain was incubated in Luria–Bertani media (BD Biosciences, USA) at 30 °C in a shaking incubator. .. After harvesting EcNR2 cells by centrifugation, we precipitated the genomic DNA using the GeneAll Exgene Cell SV Kit (GeneAll, Korea) according to the manufacturer’s protocol.

Construct:

Article Title: A vaccine candidate for post-weaning diarrhea in swine constructed with a live attenuated Salmonella delivering Escherichia coli K88ab, K88ac, FedA, and FedF fimbrial antigens and its immune responses in a murine model
Article Snippet: The attenuated S. Typhimurium strain, JOL912, was constructed by deletion of the lon, cpxR and asd genes in wild-type S. Typhimurium JOL401, as previously described ( , ). .. All strains were grown in Luria-Bertani media (LB; Becton, Dickinson and Company, Sparks, Maryland, USA) at 37°C.

Article Title: Inhibitory effects of interleukin-10 plasmid DNA on the development of atopic dermatitis-like skin lesions in NC/Nga mice
Article Snippet: The construct was transformed into chemically competent Escherichia coli DH5α cells (Invitrogen, USA). .. The transformants were selected on Luria-Bertani media (BD Biosciences, USA) supplemented with 100 µg/mL ampicillin and the plasmid DNA was extracted using a QIAprep Spin Miniprep Kit (Qiagen, USA) according to the manufacturer's instructions.

Purification:

Article Title: Novel Fabrication of MicroRNA Nanoparticle-Coated Coronary Stent for Prevention of Post-Angioplasty Restenosis
Article Snippet: .. We transformed plasmid gWiz-luciferase (Aldevron, USA) into the competent cells, Escherichia coli DH5α, using the heat shock method. gWiz-luciferase was then propagated in bacterial cultures grown in Luria-Bertani media (Becton and Dickinson company, Franklin Lakes, NJ, USA) containing 100 µg/mL of kanamycin (Biosesang Inc., Sungnam, Korea), and purified using a mini DNA-spin kit (iNtRON Biotechnology, Seongnam, Korea). ..

Article Title: Inhibitory effects of interleukin-10 plasmid DNA on the development of atopic dermatitis-like skin lesions in NC/Nga mice
Article Snippet: Purified mouse IL-10 gene was cloned into pGEM-T Easy vector (Promega, USA), and this gene was then sub-cloned into the Not I site of the mammalian expression vector pcDNA3.1 (Invitrogen, USA) to generate pcDNA3.1-IL-10 (named IL-10 plasmid DNA). .. The transformants were selected on Luria-Bertani media (BD Biosciences, USA) supplemented with 100 µg/mL ampicillin and the plasmid DNA was extracted using a QIAprep Spin Miniprep Kit (Qiagen, USA) according to the manufacturer's instructions.

Generated:

Article Title: Applying a Linear Amplification Strategy to Recombinase Polymerase Amplification for Uniform DNA Library Amplification
Article Snippet: Four were generated by random shearing of human NA12878 and E. coli EcNR2 genomic DNA. .. E. coli EcNR2 strain was incubated in Luria–Bertani media (BD Biosciences, USA) at 30 °C in a shaking incubator.

other:

Article Title: Detecting and Discriminating Shigella sonnei Using an Aptamer-Based Fluorescent Biosensor Platform
Article Snippet: The media for bacterial culture were: nutrient broth (BD Difco, Oxford, UK) for S. sonnei , S. flexneri , and S. typhimurium ; Tryptic Soy Broth (BD Difco) for S. boydii ; Luria-Bertani media (LB; BD Difco) for Escherichia coli .

Derivative Assay:

Article Title: A vaccine candidate for post-weaning diarrhea in swine constructed with a live attenuated Salmonella delivering Escherichia coli K88ab, K88ac, FedA, and FedF fimbrial antigens and its immune responses in a murine model
Article Snippet: The pBP244 Asd+ plasmid was derived from pYA3493 Asd+ plasmid by incorporating the lepB, secA, and secB genes to improve secretion of expressed heterologous antigens in Salmonella ( ). .. All strains were grown in Luria-Bertani media (LB; Becton, Dickinson and Company, Sparks, Maryland, USA) at 37°C.

E. coli Genomic Assay:

Article Title: Applying a Linear Amplification Strategy to Recombinase Polymerase Amplification for Uniform DNA Library Amplification
Article Snippet: Paragraph title: Generation of Human and E. coli Genomic DNA Library ... E. coli EcNR2 strain was incubated in Luria–Bertani media (BD Biosciences, USA) at 30 °C in a shaking incubator.

Polymerase Chain Reaction:

Article Title: Inhibitory effects of interleukin-10 plasmid DNA on the development of atopic dermatitis-like skin lesions in NC/Nga mice
Article Snippet: The amplified 549 bp PCR products were separated by 0.7% (w/v) agarose gel electrophoresis and purified using a QIAquick gel extraction kit (Qiagen, USA) according to the manufacturer's instructions. .. The transformants were selected on Luria-Bertani media (BD Biosciences, USA) supplemented with 100 µg/mL ampicillin and the plasmid DNA was extracted using a QIAprep Spin Miniprep Kit (Qiagen, USA) according to the manufacturer's instructions.

Gel Extraction:

Article Title: Inhibitory effects of interleukin-10 plasmid DNA on the development of atopic dermatitis-like skin lesions in NC/Nga mice
Article Snippet: The amplified 549 bp PCR products were separated by 0.7% (w/v) agarose gel electrophoresis and purified using a QIAquick gel extraction kit (Qiagen, USA) according to the manufacturer's instructions. .. The transformants were selected on Luria-Bertani media (BD Biosciences, USA) supplemented with 100 µg/mL ampicillin and the plasmid DNA was extracted using a QIAprep Spin Miniprep Kit (Qiagen, USA) according to the manufacturer's instructions.

Transformation Assay:

Article Title: Novel Fabrication of MicroRNA Nanoparticle-Coated Coronary Stent for Prevention of Post-Angioplasty Restenosis
Article Snippet: .. We transformed plasmid gWiz-luciferase (Aldevron, USA) into the competent cells, Escherichia coli DH5α, using the heat shock method. gWiz-luciferase was then propagated in bacterial cultures grown in Luria-Bertani media (Becton and Dickinson company, Franklin Lakes, NJ, USA) containing 100 µg/mL of kanamycin (Biosesang Inc., Sungnam, Korea), and purified using a mini DNA-spin kit (iNtRON Biotechnology, Seongnam, Korea). ..

Article Title: Inhibitory effects of interleukin-10 plasmid DNA on the development of atopic dermatitis-like skin lesions in NC/Nga mice
Article Snippet: The construct was transformed into chemically competent Escherichia coli DH5α cells (Invitrogen, USA). .. The transformants were selected on Luria-Bertani media (BD Biosciences, USA) supplemented with 100 µg/mL ampicillin and the plasmid DNA was extracted using a QIAprep Spin Miniprep Kit (Qiagen, USA) according to the manufacturer's instructions.

Recombinant:

Article Title: A vaccine candidate for post-weaning diarrhea in swine constructed with a live attenuated Salmonella delivering Escherichia coli K88ab, K88ac, FedA, and FedF fimbrial antigens and its immune responses in a murine model
Article Snippet: Escherichia coli JM109 and E. coli BL21(DE3)pLysS (hereafter referred to as E. coli BL21) were used as host cells for over-expression of recombinant fimbrial antigens. .. All strains were grown in Luria-Bertani media (LB; Becton, Dickinson and Company, Sparks, Maryland, USA) at 37°C.

Over Expression:

Article Title: A vaccine candidate for post-weaning diarrhea in swine constructed with a live attenuated Salmonella delivering Escherichia coli K88ab, K88ac, FedA, and FedF fimbrial antigens and its immune responses in a murine model
Article Snippet: Escherichia coli JM109 and E. coli BL21(DE3)pLysS (hereafter referred to as E. coli BL21) were used as host cells for over-expression of recombinant fimbrial antigens. .. All strains were grown in Luria-Bertani media (LB; Becton, Dickinson and Company, Sparks, Maryland, USA) at 37°C.

Chloramphenicol Acetyltransferase Assay:

Article Title: Inhibitory effects of interleukin-10 plasmid DNA on the development of atopic dermatitis-like skin lesions in NC/Nga mice
Article Snippet: Mouse IL-10 gene was amplified from the synthesized cDNA using polymerase chain reaction (PCR) with the following primers: forward primer, 5'-TCC ATC ATG CCT GGC TCA-3' and reverse primer, 5'-GGT GTT TTA GCT TTT CAT TTT-3' (Genebank accession number: NM_010548). .. The transformants were selected on Luria-Bertani media (BD Biosciences, USA) supplemented with 100 µg/mL ampicillin and the plasmid DNA was extracted using a QIAprep Spin Miniprep Kit (Qiagen, USA) according to the manufacturer's instructions.

Plasmid Preparation:

Article Title: A vaccine candidate for post-weaning diarrhea in swine constructed with a live attenuated Salmonella delivering Escherichia coli K88ab, K88ac, FedA, and FedF fimbrial antigens and its immune responses in a murine model
Article Snippet: The pBP244 Asd+ plasmid was derived from pYA3493 Asd+ plasmid by incorporating the lepB, secA, and secB genes to improve secretion of expressed heterologous antigens in Salmonella ( ). .. All strains were grown in Luria-Bertani media (LB; Becton, Dickinson and Company, Sparks, Maryland, USA) at 37°C.

Article Title: Novel Fabrication of MicroRNA Nanoparticle-Coated Coronary Stent for Prevention of Post-Angioplasty Restenosis
Article Snippet: .. We transformed plasmid gWiz-luciferase (Aldevron, USA) into the competent cells, Escherichia coli DH5α, using the heat shock method. gWiz-luciferase was then propagated in bacterial cultures grown in Luria-Bertani media (Becton and Dickinson company, Franklin Lakes, NJ, USA) containing 100 µg/mL of kanamycin (Biosesang Inc., Sungnam, Korea), and purified using a mini DNA-spin kit (iNtRON Biotechnology, Seongnam, Korea). ..

Article Title: Inhibitory effects of interleukin-10 plasmid DNA on the development of atopic dermatitis-like skin lesions in NC/Nga mice
Article Snippet: .. The transformants were selected on Luria-Bertani media (BD Biosciences, USA) supplemented with 100 µg/mL ampicillin and the plasmid DNA was extracted using a QIAprep Spin Miniprep Kit (Qiagen, USA) according to the manufacturer's instructions. .. Restriction analysis (Not I) and DNA sequencing confirmed the presence and orientation of the mouse IL-10 gene in the extracted plasmid DNA.

DNA Sequencing:

Article Title: Inhibitory effects of interleukin-10 plasmid DNA on the development of atopic dermatitis-like skin lesions in NC/Nga mice
Article Snippet: The transformants were selected on Luria-Bertani media (BD Biosciences, USA) supplemented with 100 µg/mL ampicillin and the plasmid DNA was extracted using a QIAprep Spin Miniprep Kit (Qiagen, USA) according to the manufacturer's instructions. .. Restriction analysis (Not I) and DNA sequencing confirmed the presence and orientation of the mouse IL-10 gene in the extracted plasmid DNA.

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