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HiMedia Laboratories luria bertani lb media
Luria Bertani Lb Media, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/luria bertani lb media/product/HiMedia Laboratories
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
luria bertani lb media - by Bioz Stars, 2020-04
95/100 stars

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Clone Assay:

Article Title: Misregulation of a DDHD Domain-containing Lipase Causes Mitochondrial Dysfunction in Yeast *
Article Snippet: The materials required for yeast extract-peptone-dextrose (YPD) and Luria-Bertani (LB) media were purchased from HiMedia. .. The materials required for yeast extract-peptone-dextrose (YPD) and Luria-Bertani (LB) media were purchased from HiMedia.

Centrifugation:

Article Title: Distinct GDP/GTP bound states of the tandem G-domains of EngA regulate ribosome binding
Article Snippet: .. Cells were grown up to OD600 0.6–0.8 at 37°C in presence of 100 µg/ml of ampicillin in Luria Bertani media (Himedia) and were induced with 50 µM isopropyl-β-D-1-thiogalacto-pyranoside (IPTG) (Sigma) at 18°C for 10 h. Cells were harvested by centrifugation at 4000×g for 5 min at 4°C and washed with Buffer A (50 mM sodium-phosphate pH 7.4 and 100 mM NaCl). .. Similarly His-YphC and its double mutant were expressed in E. coli DH5α cells.

Article Title: Recombinant human dentin matrix protein 1 (DMP1) induces the osteogenic differentiation of human periodontal ligament cells
Article Snippet: The bacterial cells were cultured in Luria Bertani (LB) media (HiMedia Laboratories, India) with 100 μg/ml ampicillin (ITW Reagents, Germany) at 37 °C with shaking at 200 rpm. .. The cells were harvested by centrifugation every 2, 4 and 6 h and suspended in 100 μl of 1X SDS loading dye buffer (125 mM Tris HCl, pH 6.8, 12% SDS, 10% glycerol, 22% β-mercaptoethanol, and 0.001% bromophenol blue).

Fluorescence:

Article Title: Misregulation of a DDHD Domain-containing Lipase Causes Mitochondrial Dysfunction in Yeast *
Article Snippet: The fluorescence-based probes for the yeast mitochondria were purchased from Life Technologies. .. The materials required for yeast extract-peptone-dextrose (YPD) and Luria-Bertani (LB) media were purchased from HiMedia.

Synthesized:

Article Title: Recombinant human dentin matrix protein 1 (DMP1) induces the osteogenic differentiation of human periodontal ligament cells
Article Snippet: 2.1 Expression of human DMP1 in E. coli The human DMP1 gene with a 6xHis tag at the C-terminus was synthesized and reported ( ). .. The bacterial cells were cultured in Luria Bertani (LB) media (HiMedia Laboratories, India) with 100 μg/ml ampicillin (ITW Reagents, Germany) at 37 °C with shaking at 200 rpm.

Mutagenesis:

Article Title: Distinct GDP/GTP bound states of the tandem G-domains of EngA regulate ribosome binding
Article Snippet: Cells were grown up to OD600 0.6–0.8 at 37°C in presence of 100 µg/ml of ampicillin in Luria Bertani media (Himedia) and were induced with 50 µM isopropyl-β-D-1-thiogalacto-pyranoside (IPTG) (Sigma) at 18°C for 10 h. Cells were harvested by centrifugation at 4000×g for 5 min at 4°C and washed with Buffer A (50 mM sodium-phosphate pH 7.4 and 100 mM NaCl). .. Similarly His-YphC and its double mutant were expressed in E. coli DH5α cells.

Construct:

Article Title: Distinct GDP/GTP bound states of the tandem G-domains of EngA regulate ribosome binding
Article Snippet: Protein expression and purification All constructs and mutants of EngA were expressed in E. coli BL21 (DE3 ). .. Cells were grown up to OD600 0.6–0.8 at 37°C in presence of 100 µg/ml of ampicillin in Luria Bertani media (Himedia) and were induced with 50 µM isopropyl-β-D-1-thiogalacto-pyranoside (IPTG) (Sigma) at 18°C for 10 h. Cells were harvested by centrifugation at 4000×g for 5 min at 4°C and washed with Buffer A (50 mM sodium-phosphate pH 7.4 and 100 mM NaCl).

Purification:

Article Title: Distinct GDP/GTP bound states of the tandem G-domains of EngA regulate ribosome binding
Article Snippet: Paragraph title: Protein expression and purification ... Cells were grown up to OD600 0.6–0.8 at 37°C in presence of 100 µg/ml of ampicillin in Luria Bertani media (Himedia) and were induced with 50 µM isopropyl-β-D-1-thiogalacto-pyranoside (IPTG) (Sigma) at 18°C for 10 h. Cells were harvested by centrifugation at 4000×g for 5 min at 4°C and washed with Buffer A (50 mM sodium-phosphate pH 7.4 and 100 mM NaCl).

Real-time Polymerase Chain Reaction:

Article Title: Misregulation of a DDHD Domain-containing Lipase Causes Mitochondrial Dysfunction in Yeast *
Article Snippet: The materials required for real-time quantitative PCR analysis were purchased from Applied Biosystems. .. The materials required for yeast extract-peptone-dextrose (YPD) and Luria-Bertani (LB) media were purchased from HiMedia.

Incubation:

Article Title: Recombinant human dentin matrix protein 1 (DMP1) induces the osteogenic differentiation of human periodontal ligament cells
Article Snippet: The bacterial cells were cultured in Luria Bertani (LB) media (HiMedia Laboratories, India) with 100 μg/ml ampicillin (ITW Reagents, Germany) at 37 °C with shaking at 200 rpm. .. After IPTG induction, the incubation continued at either 28 °C or 37 °C on a rotary shaker at 200 rpm.

Cell Culture:

Article Title: Recombinant human dentin matrix protein 1 (DMP1) induces the osteogenic differentiation of human periodontal ligament cells
Article Snippet: .. The bacterial cells were cultured in Luria Bertani (LB) media (HiMedia Laboratories, India) with 100 μg/ml ampicillin (ITW Reagents, Germany) at 37 °C with shaking at 200 rpm. .. After the OD600 of the culture reached 0.6, protein expression was induced by adding isopropyl-β-D-1-thiogalactopyranoside (IPTG) (Bio Basic, Canada) to final concentrations of 0.2, 0.5, and 1 mM.

Expressing:

Article Title: Distinct GDP/GTP bound states of the tandem G-domains of EngA regulate ribosome binding
Article Snippet: Paragraph title: Protein expression and purification ... Cells were grown up to OD600 0.6–0.8 at 37°C in presence of 100 µg/ml of ampicillin in Luria Bertani media (Himedia) and were induced with 50 µM isopropyl-β-D-1-thiogalacto-pyranoside (IPTG) (Sigma) at 18°C for 10 h. Cells were harvested by centrifugation at 4000×g for 5 min at 4°C and washed with Buffer A (50 mM sodium-phosphate pH 7.4 and 100 mM NaCl).

Article Title: Recombinant human dentin matrix protein 1 (DMP1) induces the osteogenic differentiation of human periodontal ligament cells
Article Snippet: Paragraph title: Expression of human DMP1 in E. coli ... The bacterial cells were cultured in Luria Bertani (LB) media (HiMedia Laboratories, India) with 100 μg/ml ampicillin (ITW Reagents, Germany) at 37 °C with shaking at 200 rpm.

Transformation Assay:

Article Title: Recombinant human dentin matrix protein 1 (DMP1) induces the osteogenic differentiation of human periodontal ligament cells
Article Snippet: The pET22b-DMP1 plasmid was transformed into E. coli strain Rosetta (DE3) (Novagen, Germany). .. The bacterial cells were cultured in Luria Bertani (LB) media (HiMedia Laboratories, India) with 100 μg/ml ampicillin (ITW Reagents, Germany) at 37 °C with shaking at 200 rpm.

Article Title: Misregulation of a DDHD Domain-containing Lipase Causes Mitochondrial Dysfunction in Yeast *
Article Snippet: The materials required for yeast extract-peptone-dextrose (YPD) and Luria-Bertani (LB) media were purchased from HiMedia. .. The yeast transformation kit was obtained from Clontech.

Thin Layer Chromatography:

Article Title: Misregulation of a DDHD Domain-containing Lipase Causes Mitochondrial Dysfunction in Yeast *
Article Snippet: Ltd., the Ni2+ -NTA-agarose columns were purchased from Bio-Rad, and TLC plates were obtained from Merck. .. The materials required for yeast extract-peptone-dextrose (YPD) and Luria-Bertani (LB) media were purchased from HiMedia.

Plasmid Preparation:

Article Title: Recombinant human dentin matrix protein 1 (DMP1) induces the osteogenic differentiation of human periodontal ligament cells
Article Snippet: The pET22b-DMP1 plasmid was transformed into E. coli strain Rosetta (DE3) (Novagen, Germany). .. The bacterial cells were cultured in Luria Bertani (LB) media (HiMedia Laboratories, India) with 100 μg/ml ampicillin (ITW Reagents, Germany) at 37 °C with shaking at 200 rpm.

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    HiMedia Laboratories luria bertani lb agar media
    Luria Bertani Lb Agar Media, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luria bertani lb agar media/product/HiMedia Laboratories
    Average 91 stars, based on 8 article reviews
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    luria bertani lb agar media - by Bioz Stars, 2020-04
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    HiMedia Laboratories luria bertani lb broth
    Luria Bertani Lb Broth, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luria bertani lb broth/product/HiMedia Laboratories
    Average 95 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    luria bertani lb broth - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

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