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Growcells luria bertani lb media
Luria Bertani Lb Media, supplied by Growcells, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/luria bertani lb media/product/Growcells
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
luria bertani lb media - by Bioz Stars, 2020-04
91/100 stars

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DNA Extraction:

Article Title: Droplet centrifugation, droplet DNA extraction, and rapid droplet thermocycling for simpler and faster PCR assay using wire-guided manipulations
Article Snippet: .. Conventional DNA extraction of E. coli DNA was extracted from E. coli that was cultured in Luria-Bertani (LB) media (catalog number MBPE-1050; Growcells, Irvine, CA, USA) overnight at room temperature to prevent cells from reaching death phase using Wizard Genomic DNA purification kit (catalog number A1120; Promega Bio-tek, Madison, WI, USA). .. Basically, cells were collected either by conventional centrifugation or centrifugation from the droplet manipulation system; and then subjected to the cell lysis solution to lyse cells and RNase solution to deactivate nucleases from breaking DNA and RNA.

Clone Assay:

Article Title: High Throughput Screen for Escherichia coli Twin Arginine Translocation (Tat) Inhibitors
Article Snippet: E . coli strains MC4100(DE3) and JM109 [ , , ] were used for the HTS and for cloning purposes, respectively. .. E . coli cultures were grown using Luria-Bertani (LB) media (Growcells, Irvine, CA 92618, USA) and were supplemented with ampicillin (Ap, 100 μg/mL; Sigma-Aldrich Corporation, MO, USA), chloramphenicol (Cm, 34 μg/mL) (USB Corporation, OH, USA), arabinose (Sigma-Aldrich Corporation, MO, USA), and IPTG (G-Biosciences, MO, USA) as required for growth and induction, unless otherwise indicated.

Centrifugation:

Article Title: Droplet centrifugation, droplet DNA extraction, and rapid droplet thermocycling for simpler and faster PCR assay using wire-guided manipulations
Article Snippet: Conventional DNA extraction of E. coli DNA was extracted from E. coli that was cultured in Luria-Bertani (LB) media (catalog number MBPE-1050; Growcells, Irvine, CA, USA) overnight at room temperature to prevent cells from reaching death phase using Wizard Genomic DNA purification kit (catalog number A1120; Promega Bio-tek, Madison, WI, USA). .. Basically, cells were collected either by conventional centrifugation or centrifugation from the droplet manipulation system; and then subjected to the cell lysis solution to lyse cells and RNase solution to deactivate nucleases from breaking DNA and RNA.

Cell Culture:

Article Title: Droplet centrifugation, droplet DNA extraction, and rapid droplet thermocycling for simpler and faster PCR assay using wire-guided manipulations
Article Snippet: .. Conventional DNA extraction of E. coli DNA was extracted from E. coli that was cultured in Luria-Bertani (LB) media (catalog number MBPE-1050; Growcells, Irvine, CA, USA) overnight at room temperature to prevent cells from reaching death phase using Wizard Genomic DNA purification kit (catalog number A1120; Promega Bio-tek, Madison, WI, USA). .. Basically, cells were collected either by conventional centrifugation or centrifugation from the droplet manipulation system; and then subjected to the cell lysis solution to lyse cells and RNase solution to deactivate nucleases from breaking DNA and RNA.

Incubation:

Article Title: Droplet centrifugation, droplet DNA extraction, and rapid droplet thermocycling for simpler and faster PCR assay using wire-guided manipulations
Article Snippet: Conventional DNA extraction of E. coli DNA was extracted from E. coli that was cultured in Luria-Bertani (LB) media (catalog number MBPE-1050; Growcells, Irvine, CA, USA) overnight at room temperature to prevent cells from reaching death phase using Wizard Genomic DNA purification kit (catalog number A1120; Promega Bio-tek, Madison, WI, USA). .. After an incubation period, DNA was precipitated with isopropanol and collected using centrifugation or the droplet manipulator.

DNA Purification:

Article Title: Droplet centrifugation, droplet DNA extraction, and rapid droplet thermocycling for simpler and faster PCR assay using wire-guided manipulations
Article Snippet: .. Conventional DNA extraction of E. coli DNA was extracted from E. coli that was cultured in Luria-Bertani (LB) media (catalog number MBPE-1050; Growcells, Irvine, CA, USA) overnight at room temperature to prevent cells from reaching death phase using Wizard Genomic DNA purification kit (catalog number A1120; Promega Bio-tek, Madison, WI, USA). .. Basically, cells were collected either by conventional centrifugation or centrifugation from the droplet manipulation system; and then subjected to the cell lysis solution to lyse cells and RNase solution to deactivate nucleases from breaking DNA and RNA.

Lysis:

Article Title: Droplet centrifugation, droplet DNA extraction, and rapid droplet thermocycling for simpler and faster PCR assay using wire-guided manipulations
Article Snippet: Conventional DNA extraction of E. coli DNA was extracted from E. coli that was cultured in Luria-Bertani (LB) media (catalog number MBPE-1050; Growcells, Irvine, CA, USA) overnight at room temperature to prevent cells from reaching death phase using Wizard Genomic DNA purification kit (catalog number A1120; Promega Bio-tek, Madison, WI, USA). .. Basically, cells were collected either by conventional centrifugation or centrifugation from the droplet manipulation system; and then subjected to the cell lysis solution to lyse cells and RNase solution to deactivate nucleases from breaking DNA and RNA.

Plasmid Preparation:

Article Title: High Throughput Screen for Escherichia coli Twin Arginine Translocation (Tat) Inhibitors
Article Snippet: This plasmid encodes the E . coli TatA, TatB and TatC proteins under the control of T7 promoter, and can co-exist with plasmids pTorA-mCherry-H6, pTorA-mCherry-SsrA, or pTorA-GFP-SsrA. .. E . coli cultures were grown using Luria-Bertani (LB) media (Growcells, Irvine, CA 92618, USA) and were supplemented with ampicillin (Ap, 100 μg/mL; Sigma-Aldrich Corporation, MO, USA), chloramphenicol (Cm, 34 μg/mL) (USB Corporation, OH, USA), arabinose (Sigma-Aldrich Corporation, MO, USA), and IPTG (G-Biosciences, MO, USA) as required for growth and induction, unless otherwise indicated.

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    Growcells luria bertani lb media
    Luria Bertani Lb Media, supplied by Growcells, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luria bertani lb media/product/Growcells
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    luria bertani lb media - by Bioz Stars, 2020-04
    91/100 stars
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