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Amresco luria bertani lb media
Luria Bertani Lb Media, supplied by Amresco, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/luria bertani lb media/product/Amresco
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
luria bertani lb media - by Bioz Stars, 2020-04
92/100 stars

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Purification:

Article Title: Bacterial co-expression of human Tau protein with protein kinase A and 14-3-3 for studies of 14-3-3/phospho-Tau interaction
Article Snippet: Paragraph title: Protein expression and purification ... In the latter case, 0.3 ml out of 20 ml of an overnight culture in Luria-Bertani (LB) media (Amresco) were inoculated into 20 ml of fresh LB media with appropriate antibiotics.

Protein Purification:

Article Title: Bacterial co-expression of human Tau protein with protein kinase A and 14-3-3 for studies of 14-3-3/phospho-Tau interaction
Article Snippet: In the latter case, 0.3 ml out of 20 ml of an overnight culture in Luria-Bertani (LB) media (Amresco) were inoculated into 20 ml of fresh LB media with appropriate antibiotics. .. To harvest cells, bacterial culture was centrifuged (1h, 3250g), then the pellet was suspended in 30–40 ml of an ice-cold buffer A (30 mM Tris-HCl, pH 8.0, containing 120 mM NaCl, 10 mM imidazole, 0.05 mM phenylmethanesulfonyl fluoride (PMSF), 0.001 mM leupeptin, 0.001 mM pepstatin) and stored at -80°C or immediately used for protein purification.

Concentration Assay:

Article Title: Bacterial co-expression of human Tau protein with protein kinase A and 14-3-3 for studies of 14-3-3/phospho-Tau interaction
Article Snippet: In the latter case, 0.3 ml out of 20 ml of an overnight culture in Luria-Bertani (LB) media (Amresco) were inoculated into 20 ml of fresh LB media with appropriate antibiotics. .. Culture grown until OD600 reached 0.6–0.7 and then isopropyl-β-thiogalactoside (IPTG) (Helicon) was added to a final concentration of 1 mM for 4 h or overnight cultivation at 37°C.

Expressing:

Article Title: Bacterial co-expression of human Tau protein with protein kinase A and 14-3-3 for studies of 14-3-3/phospho-Tau interaction
Article Snippet: Paragraph title: Protein expression and purification ... In the latter case, 0.3 ml out of 20 ml of an overnight culture in Luria-Bertani (LB) media (Amresco) were inoculated into 20 ml of fresh LB media with appropriate antibiotics.

Transformation Assay:

Article Title: Bacterial co-expression of human Tau protein with protein kinase A and 14-3-3 for studies of 14-3-3/phospho-Tau interaction
Article Snippet: Single colonies of transformed cells were used to produce “chemically” competent cells or to start test expressions. .. In the latter case, 0.3 ml out of 20 ml of an overnight culture in Luria-Bertani (LB) media (Amresco) were inoculated into 20 ml of fresh LB media with appropriate antibiotics.

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    Amresco luria bertani lb media
    Luria Bertani Lb Media, supplied by Amresco, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luria bertani lb media/product/Amresco
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    luria bertani lb media - by Bioz Stars, 2020-04
    92/100 stars
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