luria bertani lb agar media  (Millipore)


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    LB agar
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    l3027
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    Millipore luria bertani lb agar media

    https://www.bioz.com/result/luria bertani lb agar media/product/Millipore
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    luria bertani lb agar media - by Bioz Stars, 2020-04
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    Related Articles

    Clone Assay:

    Article Title: The contribution of Tannerella forsythia dipeptidyl aminopeptidase IV in the breakdown of collagen
    Article Snippet: Escherichia coli BL21DE3 was grown on Luria-Bertani agar (LB) (Sigma Aldrich) overnight aerobically at 37°C. .. After pET-22b+ plasmid cloning, E. coli BL21DE3 (Novagen) was grown on LB agar was supplemented with 50 mg ml−1 of ampicillin (Sigma Aldrich).

    Article Title: Identification of Dual Receptor Binding Protein Systems in Lactococcal 936 Group Phages
    Article Snippet: Paragraph title: 2.5. Block Cloning and Expression of Baseplate Proteins ... The resulting vectors were introduced into E. coli BL21(DE3) by heat shock and transformants were selected on Luria–Bertani (LB) agar plates supplemented with 10 μg/mL kanamycin (Sigma Aldrich, Wicklow, Ireland) at 37 °C.

    Centrifugation:

    Article Title: Identification of Dual Receptor Binding Protein Systems in Lactococcal 936 Group Phages
    Article Snippet: The resulting vectors were introduced into E. coli BL21(DE3) by heat shock and transformants were selected on Luria–Bertani (LB) agar plates supplemented with 10 μg/mL kanamycin (Sigma Aldrich, Wicklow, Ireland) at 37 °C. .. Cells were harvested by centrifugation at 4000× g for 30 min. Pellets were resuspended in 20 mL of lysis buffer (50 mM Tris pH 8.0, 500 mM NaCl, 5% glycerol, 1% triton-X100, 30 mM imidazole, 50 mg lysozyme), and incubated shaking at 4 °C for 30 min, followed by centrifugation at 20,000× g for 30 min.

    Amplification:

    Article Title: Occurrence of blaNDM Variants Among Enterobacteriaceae From a Neonatal Intensive Care Unit in a Northern India Hospital
    Article Snippet: Transconjugants were screened on Luria-Bertani agar supplemented with ceftazidime (10 μg ml−1 ) (Sigma-Aldrich) and sodium azide (100 μg ml−1 ) (HiMedia Laboratories, India). .. The PCR amplification confirmed the transconjugants having resistant markers.

    Synthesized:

    Article Title: Novel kojic acid-polymer-based magnetic nanocomposites for medical applications
    Article Snippet: Antimicrobial activity of KA nanocomposites Two Gram-positive bacteria (methicillin-resistant Staphylococcus aureus and Bacillus subtilis ) and one Gram-negative species (Salmonella enterica ) were used to test the ability of the synthesized nanocomposites to inhibit bacteria and fungi. .. Bacterial cultures were maintained on Luria–Bertani (LB) agar (Sigma-Aldrich).

    Article Title: Anthra[1,2-d][1,2,3]triazine-4,7,12(3H)-triones as a New Class of Antistaphylococcal Agents: Synthesis and Biological Evaluation
    Article Snippet: Bacterial Strains and Media The antimicrobial potential of all synthesized compounds was evaluated against five reference strains of bacteria: Staphylococcus aureus ATCC 25923, S. aureus ATCC 29213, S. epidermidis ATCC 12228, Pseudomonas aeruginosa ATCC 27853, and Escherichia coli ATCC 25,922 and four reference strains of human pathogenic yeasts: C. albicans ATCC 10231, C. albicans SC5314, C. krusei DSM 6128 and C. glabrata DSM 11226. .. Bacteria were routinely grown on Luria-Bertani Agar (LA, Sigma Aldrich, Schnelldorf, Germany) and yeasts were cultivated on YPD Agar (A & A Biotechnology, Gdynia, Poland).

    Blocking Assay:

    Article Title: Identification of Dual Receptor Binding Protein Systems in Lactococcal 936 Group Phages
    Article Snippet: Paragraph title: 2.5. Block Cloning and Expression of Baseplate Proteins ... The resulting vectors were introduced into E. coli BL21(DE3) by heat shock and transformants were selected on Luria–Bertani (LB) agar plates supplemented with 10 μg/mL kanamycin (Sigma Aldrich, Wicklow, Ireland) at 37 °C.

    Incubation:

    Article Title: Novel kojic acid-polymer-based magnetic nanocomposites for medical applications
    Article Snippet: Bacterial cultures were maintained on Luria–Bertani (LB) agar (Sigma-Aldrich). .. Prior to incubation with the novel nanoparticles, bacteria were cultured overnight in 5 mL of LB broth (Sigma-Aldrich) in a Certomat BS-T incubation shaker (Sartorius, Goettingen, Germany) at 37°C, set to 150 rpm until the culture reached an optical density at a wavelength of 600 nm of 1.0 (Spekol UV VIS 3.02; AnalytikJena, Jena, Germany), corresponding to 108 CFU/mL.

    Article Title: Identification of Dual Receptor Binding Protein Systems in Lactococcal 936 Group Phages
    Article Snippet: The resulting vectors were introduced into E. coli BL21(DE3) by heat shock and transformants were selected on Luria–Bertani (LB) agar plates supplemented with 10 μg/mL kanamycin (Sigma Aldrich, Wicklow, Ireland) at 37 °C. .. Targeted protein expression was induced by the addition of a final concentration of 1 mM IPTG and the culture was then incubated overnight at 20 °C.

    Article Title: Neisseria gonorrhoeae Phagosomes Delay Fusion with Primary Granules to Enhance Bacterial Survival Inside Human Neutrophils
    Article Snippet: To kill Gc prior to PMN exposure, Gc was either heat killed by incubation at 56°C for 20 min, or fixed for 15 min with 4% paraformaldehyde (PFA) (Electron Microscopy Sciences) in PBS. .. S. aureus ATCC 25923 and a Δspa mutant generated in the Newman background (obtained from E. Skaar, Vanderbilt University) were grown on Luria-Bertani (LB) agar for 16 h. S. aureus was inoculated into LB liquid broth and grown with rotation at 37°C for 20 h. In immunofluorescence experiments, prior to infection, S. aureus was prelabeled with 10 μg ml−1 4′,6-diamidino-2-phenylindole (DAPI) (Sigma) for 20 minutes in MDM at room temperature in the dark.

    Article Title: Characterization of abalone Haliotis tuberculata–Vibrio harveyi interactions in gill primary cultures
    Article Snippet: .. The two bacterial strains were cultured on Luria–Bertani Agar (Sigma, St. Louis, MO, USA) supplemented in salinity (to 20 g L−1 ) and incubated at 28 °C for 24 h. For in vitro contact, bacterial strains were washed in sterilized seawater. ..

    Article Title: Polymorphonuclear Leukocytes or Hydrogen Peroxide Enhance Biofilm Development of Mucoid Pseudomonas aeruginosa
    Article Snippet: .. Bacteria from frozen stocks were plated on Luria-Bertani (LB) agar (Sigma, St. Louis, MO) and then inoculated into LB liquid medium, which was incubated at 37°C with agitation (200 rpm). ..

    Article Title: Rational Design of Engineered Cationic Antimicrobial Peptides Consisting Exclusively of Arginine and Tryptophan, and Their Activity against Multidrug-Resistant Pathogens
    Article Snippet: To test for the bactericidal activity of the various antimicrobial peptides against these index bacteria, bacterial suspensions (∼106 CFU/ml) in 10 mM potassium phosphate buffer or phosphate buffer containing 150 mM NaCl (PBS; pH 7.2) were incubated with serial 2-fold dilutions of peptides for 1 h at 37°C. .. Serial broth dilutions were subsequently performed, and suspensions were plated on Luria-Bertani (LB) agar (Sigma, St. Louis, MO).

    Diffusion-based Assay:

    Article Title: Novel kojic acid-polymer-based magnetic nanocomposites for medical applications
    Article Snippet: Bacterial cultures were maintained on Luria–Bertani (LB) agar (Sigma-Aldrich). .. The antimicrobial activities of the synthesized nanocomposites were evaluated against the aforementioned bacteria using the agar diffusion (cup diffusion) method according to the Clinical and Laboratory Standards Institute guidelines.

    Activity Assay:

    Article Title: Novel kojic acid-polymer-based magnetic nanocomposites for medical applications
    Article Snippet: Paragraph title: Antimicrobial activity of KA nanocomposites ... Bacterial cultures were maintained on Luria–Bertani (LB) agar (Sigma-Aldrich).

    Article Title: Rational Design of Engineered Cationic Antimicrobial Peptides Consisting Exclusively of Arginine and Tryptophan, and Their Activity against Multidrug-Resistant Pathogens
    Article Snippet: To test for the bactericidal activity of the various antimicrobial peptides against these index bacteria, bacterial suspensions (∼106 CFU/ml) in 10 mM potassium phosphate buffer or phosphate buffer containing 150 mM NaCl (PBS; pH 7.2) were incubated with serial 2-fold dilutions of peptides for 1 h at 37°C. .. Serial broth dilutions were subsequently performed, and suspensions were plated on Luria-Bertani (LB) agar (Sigma, St. Louis, MO).

    Article Title: Anthra[1,2-d][1,2,3]triazine-4,7,12(3H)-triones as a New Class of Antistaphylococcal Agents: Synthesis and Biological Evaluation
    Article Snippet: Because of the positive results of preliminary tests the anti-staphylococcal activity of selected agents was also investigated against eight MSSA and four MRSA clinical isolates derived from patients with different infections ( ). .. Bacteria were routinely grown on Luria-Bertani Agar (LA, Sigma Aldrich, Schnelldorf, Germany) and yeasts were cultivated on YPD Agar (A & A Biotechnology, Gdynia, Poland).

    Infection:

    Article Title: Neisseria gonorrhoeae Phagosomes Delay Fusion with Primary Granules to Enhance Bacterial Survival Inside Human Neutrophils
    Article Snippet: .. S. aureus ATCC 25923 and a Δspa mutant generated in the Newman background (obtained from E. Skaar, Vanderbilt University) were grown on Luria-Bertani (LB) agar for 16 h. S. aureus was inoculated into LB liquid broth and grown with rotation at 37°C for 20 h. In immunofluorescence experiments, prior to infection, S. aureus was prelabeled with 10 μg ml−1 4′,6-diamidino-2-phenylindole (DAPI) (Sigma) for 20 minutes in MDM at room temperature in the dark. ..

    Expressing:

    Article Title: Specific sequences in N termini of apolipoprotein A-IV modulate its anorectic effect
    Article Snippet: .. The expression constructs were transfected into E. Coli BL-21 cells and grown on Luria-Bertani (LB) agar plates containing 30 μg/ml kana-mycin (Calbiochem, San Diego, CA) for 16 h at 37 °C. ..

    Article Title: Identification of Dual Receptor Binding Protein Systems in Lactococcal 936 Group Phages
    Article Snippet: Paragraph title: 2.5. Block Cloning and Expression of Baseplate Proteins ... The resulting vectors were introduced into E. coli BL21(DE3) by heat shock and transformants were selected on Luria–Bertani (LB) agar plates supplemented with 10 μg/mL kanamycin (Sigma Aldrich, Wicklow, Ireland) at 37 °C.

    Modification:

    Article Title: Rational Design of Engineered Cationic Antimicrobial Peptides Consisting Exclusively of Arginine and Tryptophan, and Their Activity against Multidrug-Resistant Pathogens
    Article Snippet: First, we adopted a modified CLSI bacterial killing assay identical to that previously described ( ) and used two prototypical organisms, a laboratory strain of P. aeruginosa (PAO1) and a clinical strain of methicillin-resistant S. aureus (MRSA 4003) kindly provided by Jane Marsh (University of the Pittsburgh School of Medicine). .. Serial broth dilutions were subsequently performed, and suspensions were plated on Luria-Bertani (LB) agar (Sigma, St. Louis, MO).

    Over Expression:

    Article Title: Specific sequences in N termini of apolipoprotein A-IV modulate its anorectic effect
    Article Snippet: The expression constructs were transfected into E. Coli BL-21 cells and grown on Luria-Bertani (LB) agar plates containing 30 μg/ml kana-mycin (Calbiochem, San Diego, CA) for 16 h at 37 °C. .. IPTG was added for 2 h to induce over-expression of the protein.

    Inhibition:

    Article Title: Rational Design of Engineered Cationic Antimicrobial Peptides Consisting Exclusively of Arginine and Tryptophan, and Their Activity against Multidrug-Resistant Pathogens
    Article Snippet: Paragraph title: Bacterial killing and growth inhibition assays. ... Serial broth dilutions were subsequently performed, and suspensions were plated on Luria-Bertani (LB) agar (Sigma, St. Louis, MO).

    Derivative Assay:

    Article Title: Rational Design of Engineered Cationic Antimicrobial Peptides Consisting Exclusively of Arginine and Tryptophan, and Their Activity against Multidrug-Resistant Pathogens
    Article Snippet: Serial broth dilutions were subsequently performed, and suspensions were plated on Luria-Bertani (LB) agar (Sigma, St. Louis, MO). .. Using GraphPad Prism 6.00 for Windows (GraphPad Software, San Diego, CA), the CFU/ml data (on a log scale) were plotted against peptide concentrations, and the minimum bactericidal concentration (MBC; the molar concentration of peptide reducing viable bacteria within a suspension by 3 orders of magnitude) was derived from the nonlinear regression of the survival curves using GraphPad Prism 6.00.

    Article Title: Anthra[1,2-d][1,2,3]triazine-4,7,12(3H)-triones as a New Class of Antistaphylococcal Agents: Synthesis and Biological Evaluation
    Article Snippet: Because of the positive results of preliminary tests the anti-staphylococcal activity of selected agents was also investigated against eight MSSA and four MRSA clinical isolates derived from patients with different infections ( ). .. Bacteria were routinely grown on Luria-Bertani Agar (LA, Sigma Aldrich, Schnelldorf, Germany) and yeasts were cultivated on YPD Agar (A & A Biotechnology, Gdynia, Poland).

    Conjugation Assay:

    Article Title: The Complete Sequence and Comparative Analysis of a Multidrug-Resistance and Virulence Multireplicon IncFII Plasmid pEC302/04 from an Extraintestinal Pathogenic Escherichia coli EC302/04 Indicate Extensive Diversity of IncFII Plasmids
    Article Snippet: Paragraph title: Conjugation and confirmation of transconjugants ... E. coli DH5α (pEC302/04) transconjugants were confirmed by: (a) selective plating on Luria-Bertani (LB) agar plates containing a donor-inhibiting concentration of ampicillin (100 μg) (Sigma-Aldrich) and recipient-inhibiting concentration of nalidixic acid (30 μg/ml); (b) PCR detection of plasmid-encoded bla TEM−1 gene which was present only in the donor strain EC302/04 using a previously described primer pair (Oliver et al., ); and (c) PFGE profiling of the donor, recipient cell and transconjugants which were performed according to Ho et al. ( ).

    Article Title: Occurrence of blaNDM Variants Among Enterobacteriaceae From a Neonatal Intensive Care Unit in a Northern India Hospital
    Article Snippet: Paragraph title: Conjugation experiment ... Transconjugants were screened on Luria-Bertani agar supplemented with ceftazidime (10 μg ml−1 ) (Sigma-Aldrich) and sodium azide (100 μg ml−1 ) (HiMedia Laboratories, India).

    Transfection:

    Article Title: Specific sequences in N termini of apolipoprotein A-IV modulate its anorectic effect
    Article Snippet: .. The expression constructs were transfected into E. Coli BL-21 cells and grown on Luria-Bertani (LB) agar plates containing 30 μg/ml kana-mycin (Calbiochem, San Diego, CA) for 16 h at 37 °C. ..

    Cell Culture:

    Article Title: Novel kojic acid-polymer-based magnetic nanocomposites for medical applications
    Article Snippet: Bacterial cultures were maintained on Luria–Bertani (LB) agar (Sigma-Aldrich). .. Prior to incubation with the novel nanoparticles, bacteria were cultured overnight in 5 mL of LB broth (Sigma-Aldrich) in a Certomat BS-T incubation shaker (Sartorius, Goettingen, Germany) at 37°C, set to 150 rpm until the culture reached an optical density at a wavelength of 600 nm of 1.0 (Spekol UV VIS 3.02; AnalytikJena, Jena, Germany), corresponding to 108 CFU/mL.

    Article Title: Characterization of abalone Haliotis tuberculata–Vibrio harveyi interactions in gill primary cultures
    Article Snippet: .. The two bacterial strains were cultured on Luria–Bertani Agar (Sigma, St. Louis, MO, USA) supplemented in salinity (to 20 g L−1 ) and incubated at 28 °C for 24 h. For in vitro contact, bacterial strains were washed in sterilized seawater. ..

    Generated:

    Article Title: Neisseria gonorrhoeae Phagosomes Delay Fusion with Primary Granules to Enhance Bacterial Survival Inside Human Neutrophils
    Article Snippet: .. S. aureus ATCC 25923 and a Δspa mutant generated in the Newman background (obtained from E. Skaar, Vanderbilt University) were grown on Luria-Bertani (LB) agar for 16 h. S. aureus was inoculated into LB liquid broth and grown with rotation at 37°C for 20 h. In immunofluorescence experiments, prior to infection, S. aureus was prelabeled with 10 μg ml−1 4′,6-diamidino-2-phenylindole (DAPI) (Sigma) for 20 minutes in MDM at room temperature in the dark. ..

    other:

    Article Title: The Lysobacter capsici AZ78 Genome Has a Gene Pool Enabling it to Interact Successfully with Phytopathogenic Microorganisms and Environmental Factors
    Article Snippet: Microorganisms L. capsici AZ78 was stored at length in glycerol 40% at −80°C and routinely grown on Luria-Bertani Agar at 27°C (LBA, Sigma-Aldrich, USA).

    Polymerase Chain Reaction:

    Article Title: The Complete Sequence and Comparative Analysis of a Multidrug-Resistance and Virulence Multireplicon IncFII Plasmid pEC302/04 from an Extraintestinal Pathogenic Escherichia coli EC302/04 Indicate Extensive Diversity of IncFII Plasmids
    Article Snippet: .. E. coli DH5α (pEC302/04) transconjugants were confirmed by: (a) selective plating on Luria-Bertani (LB) agar plates containing a donor-inhibiting concentration of ampicillin (100 μg) (Sigma-Aldrich) and recipient-inhibiting concentration of nalidixic acid (30 μg/ml); (b) PCR detection of plasmid-encoded bla TEM−1 gene which was present only in the donor strain EC302/04 using a previously described primer pair (Oliver et al., ); and (c) PFGE profiling of the donor, recipient cell and transconjugants which were performed according to Ho et al. ( ). .. The conjugation frequency (number of transconjugants per donor cell) was calculated using the averages of two biological and three technical replicates.

    Article Title: Identification of Dual Receptor Binding Protein Systems in Lactococcal 936 Group Phages
    Article Snippet: Baseplate constructs incorporating gene deletions were constructed via SOEing PCR as described previously [ ], with primers outlined in . .. The resulting vectors were introduced into E. coli BL21(DE3) by heat shock and transformants were selected on Luria–Bertani (LB) agar plates supplemented with 10 μg/mL kanamycin (Sigma Aldrich, Wicklow, Ireland) at 37 °C.

    Article Title: Occurrence of blaNDM Variants Among Enterobacteriaceae From a Neonatal Intensive Care Unit in a Northern India Hospital
    Article Snippet: Transconjugants were screened on Luria-Bertani agar supplemented with ceftazidime (10 μg ml−1 ) (Sigma-Aldrich) and sodium azide (100 μg ml−1 ) (HiMedia Laboratories, India). .. The PCR amplification confirmed the transconjugants having resistant markers.

    Time-Kill Assay:

    Article Title: Anthra[1,2-d][1,2,3]triazine-4,7,12(3H)-triones as a New Class of Antistaphylococcal Agents: Synthesis and Biological Evaluation
    Article Snippet: Bacteria were routinely grown on Luria-Bertani Agar (LA, Sigma Aldrich, Schnelldorf, Germany) and yeasts were cultivated on YPD Agar (A & A Biotechnology, Gdynia, Poland). .. For staphylococcal biofilm formation, TSB (Sigma Aldrich) liquid medium supplemented with 2.5% of glucose was used and Time-Kill assay was performed in Mueller-Hinton Broth 2.

    Immunofluorescence:

    Article Title: Neisseria gonorrhoeae Phagosomes Delay Fusion with Primary Granules to Enhance Bacterial Survival Inside Human Neutrophils
    Article Snippet: .. S. aureus ATCC 25923 and a Δspa mutant generated in the Newman background (obtained from E. Skaar, Vanderbilt University) were grown on Luria-Bertani (LB) agar for 16 h. S. aureus was inoculated into LB liquid broth and grown with rotation at 37°C for 20 h. In immunofluorescence experiments, prior to infection, S. aureus was prelabeled with 10 μg ml−1 4′,6-diamidino-2-phenylindole (DAPI) (Sigma) for 20 minutes in MDM at room temperature in the dark. ..

    Transmission Electron Microscopy:

    Article Title: The Complete Sequence and Comparative Analysis of a Multidrug-Resistance and Virulence Multireplicon IncFII Plasmid pEC302/04 from an Extraintestinal Pathogenic Escherichia coli EC302/04 Indicate Extensive Diversity of IncFII Plasmids
    Article Snippet: .. E. coli DH5α (pEC302/04) transconjugants were confirmed by: (a) selective plating on Luria-Bertani (LB) agar plates containing a donor-inhibiting concentration of ampicillin (100 μg) (Sigma-Aldrich) and recipient-inhibiting concentration of nalidixic acid (30 μg/ml); (b) PCR detection of plasmid-encoded bla TEM−1 gene which was present only in the donor strain EC302/04 using a previously described primer pair (Oliver et al., ); and (c) PFGE profiling of the donor, recipient cell and transconjugants which were performed according to Ho et al. ( ). .. The conjugation frequency (number of transconjugants per donor cell) was calculated using the averages of two biological and three technical replicates.

    Electron Microscopy:

    Article Title: Neisseria gonorrhoeae Phagosomes Delay Fusion with Primary Granules to Enhance Bacterial Survival Inside Human Neutrophils
    Article Snippet: To kill Gc prior to PMN exposure, Gc was either heat killed by incubation at 56°C for 20 min, or fixed for 15 min with 4% paraformaldehyde (PFA) (Electron Microscopy Sciences) in PBS. .. S. aureus ATCC 25923 and a Δspa mutant generated in the Newman background (obtained from E. Skaar, Vanderbilt University) were grown on Luria-Bertani (LB) agar for 16 h. S. aureus was inoculated into LB liquid broth and grown with rotation at 37°C for 20 h. In immunofluorescence experiments, prior to infection, S. aureus was prelabeled with 10 μg ml−1 4′,6-diamidino-2-phenylindole (DAPI) (Sigma) for 20 minutes in MDM at room temperature in the dark.

    Mutagenesis:

    Article Title: Neisseria gonorrhoeae Phagosomes Delay Fusion with Primary Granules to Enhance Bacterial Survival Inside Human Neutrophils
    Article Snippet: .. S. aureus ATCC 25923 and a Δspa mutant generated in the Newman background (obtained from E. Skaar, Vanderbilt University) were grown on Luria-Bertani (LB) agar for 16 h. S. aureus was inoculated into LB liquid broth and grown with rotation at 37°C for 20 h. In immunofluorescence experiments, prior to infection, S. aureus was prelabeled with 10 μg ml−1 4′,6-diamidino-2-phenylindole (DAPI) (Sigma) for 20 minutes in MDM at room temperature in the dark. ..

    Isolation:

    Article Title: Characterization of abalone Haliotis tuberculata–Vibrio harveyi interactions in gill primary cultures
    Article Snippet: Two V. harveyi strains were used for in vitro pathogenicity assays: a virulent strain ORM4 isolated from a moribund abalone in 1998 (Nicolas et al. ) and a non pathogenic strain LMG7890 (former V. carchariae type strain) isolated from a dead brown shark Carcharinus plumbeus (Colwell ). .. The two bacterial strains were cultured on Luria–Bertani Agar (Sigma, St. Louis, MO, USA) supplemented in salinity (to 20 g L−1 ) and incubated at 28 °C for 24 h. For in vitro contact, bacterial strains were washed in sterilized seawater.

    Article Title: Rational Design of Engineered Cationic Antimicrobial Peptides Consisting Exclusively of Arginine and Tryptophan, and Their Activity against Multidrug-Resistant Pathogens
    Article Snippet: This panel included two National Institutes of Health (NIH) reference strains, MRSA USA100 and MRSA USA300, and nine strains isolated from patients at UPMC Presbyterian Hospital consisting of MDR, XDR, and pandrug-resistant (PDR) strains of the Gram-negative pathogens Acinetobacter baumannii , Klebsiella pneumoniae , and P. aeruginosa ( ). .. Serial broth dilutions were subsequently performed, and suspensions were plated on Luria-Bertani (LB) agar (Sigma, St. Louis, MO).

    Labeling:

    Article Title: Neisseria gonorrhoeae Phagosomes Delay Fusion with Primary Granules to Enhance Bacterial Survival Inside Human Neutrophils
    Article Snippet: Gc was labeled with 5 μg ml−1 carboxyfluorescein diacetate succinimidyl ester (CFSE) for 20 min at 37°C. .. S. aureus ATCC 25923 and a Δspa mutant generated in the Newman background (obtained from E. Skaar, Vanderbilt University) were grown on Luria-Bertani (LB) agar for 16 h. S. aureus was inoculated into LB liquid broth and grown with rotation at 37°C for 20 h. In immunofluorescence experiments, prior to infection, S. aureus was prelabeled with 10 μg ml−1 4′,6-diamidino-2-phenylindole (DAPI) (Sigma) for 20 minutes in MDM at room temperature in the dark.

    Purification:

    Article Title: Specific sequences in N termini of apolipoprotein A-IV modulate its anorectic effect
    Article Snippet: Paragraph title: 2.3. Protein expression and purification ... The expression constructs were transfected into E. Coli BL-21 cells and grown on Luria-Bertani (LB) agar plates containing 30 μg/ml kana-mycin (Calbiochem, San Diego, CA) for 16 h at 37 °C.

    Sequencing:

    Article Title: Rational Design of Engineered Cationic Antimicrobial Peptides Consisting Exclusively of Arginine and Tryptophan, and Their Activity against Multidrug-Resistant Pathogens
    Article Snippet: Some features of the standard assay endorsed by the Clinical and Laboratory Standards Institute (CLSI) were modified to satisfy the objective of the present study, which was to compare a repeated sequence folded into amphipathic helical structures for the effects of length, NaCl, and pH on bactericidal activity. .. Serial broth dilutions were subsequently performed, and suspensions were plated on Luria-Bertani (LB) agar (Sigma, St. Louis, MO).

    Construct:

    Article Title: Specific sequences in N termini of apolipoprotein A-IV modulate its anorectic effect
    Article Snippet: .. The expression constructs were transfected into E. Coli BL-21 cells and grown on Luria-Bertani (LB) agar plates containing 30 μg/ml kana-mycin (Calbiochem, San Diego, CA) for 16 h at 37 °C. ..

    Article Title: Identification of Dual Receptor Binding Protein Systems in Lactococcal 936 Group Phages
    Article Snippet: Baseplate constructs incorporating gene deletions were constructed via SOEing PCR as described previously [ ], with primers outlined in . .. The resulting vectors were introduced into E. coli BL21(DE3) by heat shock and transformants were selected on Luria–Bertani (LB) agar plates supplemented with 10 μg/mL kanamycin (Sigma Aldrich, Wicklow, Ireland) at 37 °C.

    Plasmid Preparation:

    Article Title: The contribution of Tannerella forsythia dipeptidyl aminopeptidase IV in the breakdown of collagen
    Article Snippet: Escherichia coli BL21DE3 was grown on Luria-Bertani agar (LB) (Sigma Aldrich) overnight aerobically at 37°C. .. After pET-22b+ plasmid cloning, E. coli BL21DE3 (Novagen) was grown on LB agar was supplemented with 50 mg ml−1 of ampicillin (Sigma Aldrich).

    Article Title: The Complete Sequence and Comparative Analysis of a Multidrug-Resistance and Virulence Multireplicon IncFII Plasmid pEC302/04 from an Extraintestinal Pathogenic Escherichia coli EC302/04 Indicate Extensive Diversity of IncFII Plasmids
    Article Snippet: .. E. coli DH5α (pEC302/04) transconjugants were confirmed by: (a) selective plating on Luria-Bertani (LB) agar plates containing a donor-inhibiting concentration of ampicillin (100 μg) (Sigma-Aldrich) and recipient-inhibiting concentration of nalidixic acid (30 μg/ml); (b) PCR detection of plasmid-encoded bla TEM−1 gene which was present only in the donor strain EC302/04 using a previously described primer pair (Oliver et al., ); and (c) PFGE profiling of the donor, recipient cell and transconjugants which were performed according to Ho et al. ( ). .. The conjugation frequency (number of transconjugants per donor cell) was calculated using the averages of two biological and three technical replicates.

    Software:

    Article Title: Rational Design of Engineered Cationic Antimicrobial Peptides Consisting Exclusively of Arginine and Tryptophan, and Their Activity against Multidrug-Resistant Pathogens
    Article Snippet: Serial broth dilutions were subsequently performed, and suspensions were plated on Luria-Bertani (LB) agar (Sigma, St. Louis, MO). .. Using GraphPad Prism 6.00 for Windows (GraphPad Software, San Diego, CA), the CFU/ml data (on a log scale) were plotted against peptide concentrations, and the minimum bactericidal concentration (MBC; the molar concentration of peptide reducing viable bacteria within a suspension by 3 orders of magnitude) was derived from the nonlinear regression of the survival curves using GraphPad Prism 6.00.

    Positron Emission Tomography:

    Article Title: The contribution of Tannerella forsythia dipeptidyl aminopeptidase IV in the breakdown of collagen
    Article Snippet: Escherichia coli BL21DE3 was grown on Luria-Bertani agar (LB) (Sigma Aldrich) overnight aerobically at 37°C. .. After pET-22b+ plasmid cloning, E. coli BL21DE3 (Novagen) was grown on LB agar was supplemented with 50 mg ml−1 of ampicillin (Sigma Aldrich).

    In Vitro:

    Article Title: Characterization of abalone Haliotis tuberculata–Vibrio harveyi interactions in gill primary cultures
    Article Snippet: .. The two bacterial strains were cultured on Luria–Bertani Agar (Sigma, St. Louis, MO, USA) supplemented in salinity (to 20 g L−1 ) and incubated at 28 °C for 24 h. For in vitro contact, bacterial strains were washed in sterilized seawater. ..

    Concentration Assay:

    Article Title: The Complete Sequence and Comparative Analysis of a Multidrug-Resistance and Virulence Multireplicon IncFII Plasmid pEC302/04 from an Extraintestinal Pathogenic Escherichia coli EC302/04 Indicate Extensive Diversity of IncFII Plasmids
    Article Snippet: .. E. coli DH5α (pEC302/04) transconjugants were confirmed by: (a) selective plating on Luria-Bertani (LB) agar plates containing a donor-inhibiting concentration of ampicillin (100 μg) (Sigma-Aldrich) and recipient-inhibiting concentration of nalidixic acid (30 μg/ml); (b) PCR detection of plasmid-encoded bla TEM−1 gene which was present only in the donor strain EC302/04 using a previously described primer pair (Oliver et al., ); and (c) PFGE profiling of the donor, recipient cell and transconjugants which were performed according to Ho et al. ( ). .. The conjugation frequency (number of transconjugants per donor cell) was calculated using the averages of two biological and three technical replicates.

    Article Title: Identification of Dual Receptor Binding Protein Systems in Lactococcal 936 Group Phages
    Article Snippet: The resulting vectors were introduced into E. coli BL21(DE3) by heat shock and transformants were selected on Luria–Bertani (LB) agar plates supplemented with 10 μg/mL kanamycin (Sigma Aldrich, Wicklow, Ireland) at 37 °C. .. Targeted protein expression was induced by the addition of a final concentration of 1 mM IPTG and the culture was then incubated overnight at 20 °C.

    Article Title: Rational Design of Engineered Cationic Antimicrobial Peptides Consisting Exclusively of Arginine and Tryptophan, and Their Activity against Multidrug-Resistant Pathogens
    Article Snippet: Serial broth dilutions were subsequently performed, and suspensions were plated on Luria-Bertani (LB) agar (Sigma, St. Louis, MO). .. Using GraphPad Prism 6.00 for Windows (GraphPad Software, San Diego, CA), the CFU/ml data (on a log scale) were plotted against peptide concentrations, and the minimum bactericidal concentration (MBC; the molar concentration of peptide reducing viable bacteria within a suspension by 3 orders of magnitude) was derived from the nonlinear regression of the survival curves using GraphPad Prism 6.00.

    Article Title: Anthra[1,2-d][1,2,3]triazine-4,7,12(3H)-triones as a New Class of Antistaphylococcal Agents: Synthesis and Biological Evaluation
    Article Snippet: Bacteria were routinely grown on Luria-Bertani Agar (LA, Sigma Aldrich, Schnelldorf, Germany) and yeasts were cultivated on YPD Agar (A & A Biotechnology, Gdynia, Poland). .. In the case of bacteria, the Minimum Inhibitory Concentration (MIC) values were determined using Mueller-Hinton Broth 2 (MHB2, Sigma Aldrich) liquid medium—and determination of the same parameter for yeasts was performed using RPMI 1640 medium (Sigma Aldrich) supplemented with 2% glucose (Sigma Aldrich) and buffered to pH 7.0 with a MOPS buffer (3-N -morpholinopropanesulfonic acid) (Sigma Aldrich).

    Lysis:

    Article Title: Identification of Dual Receptor Binding Protein Systems in Lactococcal 936 Group Phages
    Article Snippet: The resulting vectors were introduced into E. coli BL21(DE3) by heat shock and transformants were selected on Luria–Bertani (LB) agar plates supplemented with 10 μg/mL kanamycin (Sigma Aldrich, Wicklow, Ireland) at 37 °C. .. Cells were harvested by centrifugation at 4000× g for 30 min. Pellets were resuspended in 20 mL of lysis buffer (50 mM Tris pH 8.0, 500 mM NaCl, 5% glycerol, 1% triton-X100, 30 mM imidazole, 50 mg lysozyme), and incubated shaking at 4 °C for 30 min, followed by centrifugation at 20,000× g for 30 min.

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    Millipore agar plate preparation lysogeny broth
    Agar Plate Preparation Lysogeny Broth, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agar plate preparation lysogeny broth/product/Millipore
    Average 93 stars, based on 1 article reviews
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    agar plate preparation lysogeny broth - by Bioz Stars, 2020-04
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