Structured Review

HiMedia Laboratories luria bertani broth
Luria Bertani Broth, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 8 article reviews
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luria bertani broth - by Bioz Stars, 2020-04
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Clone Assay:

Article Title: A novel medium-throughput biological assay system for HTLV-1 infectivity and drug discovery
Article Snippet: After that, it (SV40-EGFP) was cloned, simultaneously when the CMV promoter was removed in pCDNA3.1+Hyg between the Xb aI- Bg lII sites (Thermo Fisher Scientific, LithuaniaLtd). .. Once the selected colonies were grown in the Luria-Bertani broth (LB, Himedia) overnight, the recombinant vector was purified by the QIAGEN Plasmid Mini Kit (Qiagen, Germany).

Amplification:

Article Title: Emergence of integron borne PER-1 mediated extended spectrum cephalosporin resistance among nosocomial isolates of Gram-negative bacilli
Article Snippet: PCR amplification was performed using 30 µl of total reaction volume. .. PCR reactions were run under the following conditions: initial denaturation at 95°C for two min, 30 cycles of 95 °C for 20 sec, 52°C for 45 sec, 72°C for one min and final extension at 72°C for five min. Plasmid preparation : All ESBL positive bacterial isolates were cultured in Luria-Bertani broth (Hi-Media, Mumbai, India) containing 1µg/ml of cefotaxime.

Article Title: Bioremediation of Agro-Based Pulp Mill Effluent by Microbial Consortium Comprising Autochthonous Bacteria
Article Snippet: For 16 S rRNA sequencing, the bacterial culture was inoculated in Luria Bertani broth (Hi-Media). .. Universal primers 16 sF (CAGCAGCCGCGGTAATAC) and 16 sR (TACGGCTACCTTGTTACG) were used for amplification of 16 S rRNA gene.

Article Title: A novel medium-throughput biological assay system for HTLV-1 infectivity and drug discovery
Article Snippet: Firstly, SV40 promoter from pCDNA3.1Hyg+ and fragment of pEGFP-N1 containing the EGFP were amplified by PCR using the following set of primers: SV40 FWD =5’ CAAGATCTGGTTAAAAAATGAGCTGA-TTTA 3’, SV40 Rev =5’TTGCTCACCATCACGT GCTGATCA-GAT 3’. .. Once the selected colonies were grown in the Luria-Bertani broth (LB, Himedia) overnight, the recombinant vector was purified by the QIAGEN Plasmid Mini Kit (Qiagen, Germany).

Positive Control:

Article Title: Prevalence and antibacterial resistance patterns of extended-spectrum beta-lactamase producing Gram-negative bacteria isolated from ocular infections
Article Snippet: Preparation of template DNA from isolated Gram-negative bacteria A single colony of each nonduplicate Gram-negative isolate was inoculated from MacConkey agar into 5 ml of Luria-Bertani broth (M1245; Hi-Media Laboratories Pvt. .. Along with the test isolates, DNA of bla CTX-M -gene carrying E. coli 4712 (positive control for bla CTX-M-15 ; GenBank accession number KC200080), DNA of bla SHV -gene carrying K. pneumoniae (ATCC 700603) (positive control for bla SHV -gene),[ ] DNA of bla TEM -gene carrying E. coli (ATCC 35218) (positive control for bla TEM -gene),[ ] DNA of bla OXA -gene laboratory isolate of K. pneumoniae 7888 (positive control for bla OXA -gene GenBank accession number JN565742), and DNA of non-ESBL-producing organism, E. coli ATCC 25922 were also extracted for performing positive and negative control during each mPCR.

Construct:

Article Title: A novel medium-throughput biological assay system for HTLV-1 infectivity and drug discovery
Article Snippet: Paragraph title: Plasmids and constructs ... Once the selected colonies were grown in the Luria-Bertani broth (LB, Himedia) overnight, the recombinant vector was purified by the QIAGEN Plasmid Mini Kit (Qiagen, Germany).

Incubation:

Article Title: Prevalence and antibacterial resistance patterns of extended-spectrum beta-lactamase producing Gram-negative bacteria isolated from ocular infections
Article Snippet: Preparation of template DNA from isolated Gram-negative bacteria A single colony of each nonduplicate Gram-negative isolate was inoculated from MacConkey agar into 5 ml of Luria-Bertani broth (M1245; Hi-Media Laboratories Pvt. .. Ltd., Mumbai, India) and incubated for 20 h at 37°C.

Article Title: Curcumin Quantum Dots Mediated Degradation of Bacterial Biofilms
Article Snippet: Briefly, overnight cultures of staphylococcal strains [S. aureus (ATCC 29213), S. epidermidis (ATCC 35984 and ATCC 35983), and MRSA were diluted 1:100 in 50% brain heart infusion (BHI) broth (HiMedia laboratories, India)/glucose (4%) at pH 6.6, whereas, E. coli (ATCC 25922) and P. aeruginosa (ATCC 25619) were grown in Luria Bertani broth (HiMedia laboratories, India)]. .. Plates were incubated at 37°C without shaking for 24 h in one case where its biofilm inhibitory actions were investigated while, action on preformed biofilms were investigated after 48 h of incubation.

Article Title: Multidrug-Resistance and Extended Spectrum Beta-Lactamase Production in Uropathogenic E. Coli which were Isolated from Hospitalized Patients in Kolkata, India
Article Snippet: .. The media which were used in the study included, MacConkey’s Agar, Eosin Methylene Blue Agar and Luria Bertani Broth (Hi-Media, Mumbai).The cultures were incubated at 37ºC for 24 hrs respectively. .. These E. coli isolates were subcultured on Luria Bertani broth and they were maintained on Luria bertani agar plates at 4ºC for further studies.

Article Title: Emergence of integron borne PER-1 mediated extended spectrum cephalosporin resistance among nosocomial isolates of Gram-negative bacilli
Article Snippet: PCR reactions were run under the following conditions: initial denaturation at 95°C for two min, 30 cycles of 95 °C for 20 sec, 52°C for 45 sec, 72°C for one min and final extension at 72°C for five min. Plasmid preparation : All ESBL positive bacterial isolates were cultured in Luria-Bertani broth (Hi-Media, Mumbai, India) containing 1µg/ml of cefotaxime. .. Cultures were incubated on shaker incubator overnight at 37°C.

Article Title: A comparative study of coastal and clinical isolates ofPseudomonas aeruginosa
Article Snippet: Screening of proteolytic and haemolytic activities To determine the proteolytic and haemolytic activities, 5 μL of overnight cultures grown in Luria-Bertani broth (Himedia, India) were spotted on casein milk agar and 5% blood agar plates, respectively ( ). .. After 48 h of incubation at room temperature, activities were measured as the diameters of clear halos formed around bacterial colonies.

Activity Assay:

Article Title: Curcumin Quantum Dots Mediated Degradation of Bacterial Biofilms
Article Snippet: Paragraph title: Antibiofilm Activity Determination Using Tissue Culture Plate Assay (TCP) ... Briefly, overnight cultures of staphylococcal strains [S. aureus (ATCC 29213), S. epidermidis (ATCC 35984 and ATCC 35983), and MRSA were diluted 1:100 in 50% brain heart infusion (BHI) broth (HiMedia laboratories, India)/glucose (4%) at pH 6.6, whereas, E. coli (ATCC 25922) and P. aeruginosa (ATCC 25619) were grown in Luria Bertani broth (HiMedia laboratories, India)].

Touchdown PCR:

Article Title: A novel medium-throughput biological assay system for HTLV-1 infectivity and drug discovery
Article Snippet: Then, the integration of SV40 promoter was done using the Touchdown PCR protocol by the SV40 FWD and EGFP Rev as forward and reverse primers at the EGFP gene upstream ( ). .. Once the selected colonies were grown in the Luria-Bertani broth (LB, Himedia) overnight, the recombinant vector was purified by the QIAGEN Plasmid Mini Kit (Qiagen, Germany).

Modification:

Article Title: Phagocytosis of full-length Tau oligomers by Actin-remodeling of activated microglia
Article Snippet: Chemical and reagents Luria-Bertani broth (Himedia), ampicillin, NaCl, phenylmethylsulfonylfluoride (PMSF), MgCl2, ammonium persulfate (APS), heparin (17,500 Da), DMSO, and methanol were purchased from MP Biomedicals; IPTG and dithiothreitol (DTT) from Calbiochem; MES, BES, glutaraldehyde, and SDS from Sigma; and EGTA, protease inhibitor cocktail, Tris base, 40% acrylamide, and TEMED from Invitrogen. .. For cell culture studies, Dulbecco’s modified Eagle’s media (DMEM), fetal bovine serum (FBS), horse serum, phosphate buffer saline (PBS, cell biology grade), trypsin-EDTA, and penicillin-streptomycin were purchased from Invitrogen.

Transformation Assay:

Article Title: Occurrence of blaDHA-1 mediated cephalosporin resistance in Escherichia coli and their transcriptional response against cephalosporin stress: a report from India
Article Snippet: Paragraph title: Transferability of bla DHA gene by transformation and conjugation ... An overnight culture of the bacteria was diluted in Luria–Bertani broth (Hi-Media, Mumbai, India) and was grown at 37 °C till the O.D. of the recipient and donor culture reached 0.8–0.9 at A600 .

Article Title: Genetic Acquisition of NDM Gene Offers Sustainability among Clinical Isolates of Pseudomonas aeruginosa in Clinical Settings
Article Snippet: Transferability & plasmid profiling Horizontal transferability of bla NDM was investigated by transformation assay. .. Overnight culture of bacteria were diluted in Luria Bertani broth (Hi-Media, Mumbai, India) and was grown at 37°C till the O.D. of the recipient and donor culture reached 0.8–0.9 at A600 .

Article Title: Emergence of integron borne PER-1 mediated extended spectrum cephalosporin resistance among nosocomial isolates of Gram-negative bacilli
Article Snippet: PCR reactions were run under the following conditions: initial denaturation at 95°C for two min, 30 cycles of 95 °C for 20 sec, 52°C for 45 sec, 72°C for one min and final extension at 72°C for five min. Plasmid preparation : All ESBL positive bacterial isolates were cultured in Luria-Bertani broth (Hi-Media, Mumbai, India) containing 1µg/ml of cefotaxime. .. Gene transferability of blaESBL gene by transformation and conjugation : Transformation was carried out using Escherichia coli JM107 (Fermentas, USA) as recipient .

Countercurrent Chromatography:

Article Title: Emergence of integron borne PER-1 mediated extended spectrum cephalosporin resistance among nosocomial isolates of Gram-negative bacilli
Article Snippet: Characterization of integrons by integrase gene PCR : For characterization of class 1 and class 2 integrons, integrase gene PCR assays were performed by using primers Int1F (CAG TGG ACA TAA GCC TGT TC), Int1R (CCC GAG GCA TAG ACT GTA) and Int2F (TTG CGA GTA TCC ATA ACC TG), Int2R (TTA CCT GCA CTG GAT TAA GC) . .. PCR reactions were run under the following conditions: initial denaturation at 95°C for two min, 30 cycles of 95 °C for 20 sec, 52°C for 45 sec, 72°C for one min and final extension at 72°C for five min. Plasmid preparation : All ESBL positive bacterial isolates were cultured in Luria-Bertani broth (Hi-Media, Mumbai, India) containing 1µg/ml of cefotaxime.

Conjugation Assay:

Article Title: Occurrence of blaDHA-1 mediated cephalosporin resistance in Escherichia coli and their transcriptional response against cephalosporin stress: a report from India
Article Snippet: Paragraph title: Transferability of bla DHA gene by transformation and conjugation ... An overnight culture of the bacteria was diluted in Luria–Bertani broth (Hi-Media, Mumbai, India) and was grown at 37 °C till the O.D. of the recipient and donor culture reached 0.8–0.9 at A600 .

Article Title: Genetic Acquisition of NDM Gene Offers Sustainability among Clinical Isolates of Pseudomonas aeruginosa in Clinical Settings
Article Snippet: Conjugation experiment was carried out between clinical isolates as donors and streptomycin resistance E.coli recipient strain B (Genei, Banglore, India). .. Overnight culture of bacteria were diluted in Luria Bertani broth (Hi-Media, Mumbai, India) and was grown at 37°C till the O.D. of the recipient and donor culture reached 0.8–0.9 at A600 .

Article Title: Emergence of integron borne PER-1 mediated extended spectrum cephalosporin resistance among nosocomial isolates of Gram-negative bacilli
Article Snippet: PCR reactions were run under the following conditions: initial denaturation at 95°C for two min, 30 cycles of 95 °C for 20 sec, 52°C for 45 sec, 72°C for one min and final extension at 72°C for five min. Plasmid preparation : All ESBL positive bacterial isolates were cultured in Luria-Bertani broth (Hi-Media, Mumbai, India) containing 1µg/ml of cefotaxime. .. Gene transferability of blaESBL gene by transformation and conjugation : Transformation was carried out using Escherichia coli JM107 (Fermentas, USA) as recipient .

Protease Inhibitor:

Article Title: Phagocytosis of full-length Tau oligomers by Actin-remodeling of activated microglia
Article Snippet: .. Chemical and reagents Luria-Bertani broth (Himedia), ampicillin, NaCl, phenylmethylsulfonylfluoride (PMSF), MgCl2, ammonium persulfate (APS), heparin (17,500 Da), DMSO, and methanol were purchased from MP Biomedicals; IPTG and dithiothreitol (DTT) from Calbiochem; MES, BES, glutaraldehyde, and SDS from Sigma; and EGTA, protease inhibitor cocktail, Tris base, 40% acrylamide, and TEMED from Invitrogen. .. For cell culture studies, Dulbecco’s modified Eagle’s media (DMEM), fetal bovine serum (FBS), horse serum, phosphate buffer saline (PBS, cell biology grade), trypsin-EDTA, and penicillin-streptomycin were purchased from Invitrogen.

Cell Culture:

Article Title: Phagocytosis of full-length Tau oligomers by Actin-remodeling of activated microglia
Article Snippet: Chemical and reagents Luria-Bertani broth (Himedia), ampicillin, NaCl, phenylmethylsulfonylfluoride (PMSF), MgCl2, ammonium persulfate (APS), heparin (17,500 Da), DMSO, and methanol were purchased from MP Biomedicals; IPTG and dithiothreitol (DTT) from Calbiochem; MES, BES, glutaraldehyde, and SDS from Sigma; and EGTA, protease inhibitor cocktail, Tris base, 40% acrylamide, and TEMED from Invitrogen. .. For cell culture studies, Dulbecco’s modified Eagle’s media (DMEM), fetal bovine serum (FBS), horse serum, phosphate buffer saline (PBS, cell biology grade), trypsin-EDTA, and penicillin-streptomycin were purchased from Invitrogen.

Article Title: Anti-virulence potential of eugenol-rich fraction of Syzygium aromaticum against multidrug resistant uropathogens isolated from catheterized patients
Article Snippet: .. All the test organisms were cultured in Luria-Bertani broth (Himedia Laboratories Pvt Ltd, Bangalore, India, pH 7.0) and maintained under 120 rpm at 37 °C. ..

Article Title: Emergence of integron borne PER-1 mediated extended spectrum cephalosporin resistance among nosocomial isolates of Gram-negative bacilli
Article Snippet: .. PCR reactions were run under the following conditions: initial denaturation at 95°C for two min, 30 cycles of 95 °C for 20 sec, 52°C for 45 sec, 72°C for one min and final extension at 72°C for five min. Plasmid preparation : All ESBL positive bacterial isolates were cultured in Luria-Bertani broth (Hi-Media, Mumbai, India) containing 1µg/ml of cefotaxime. .. Cultures were incubated on shaker incubator overnight at 37°C.

Sequencing:

Article Title: Bioremediation of Agro-Based Pulp Mill Effluent by Microbial Consortium Comprising Autochthonous Bacteria
Article Snippet: .. For 16 S rRNA sequencing, the bacterial culture was inoculated in Luria Bertani broth (Hi-Media). .. Overnight grown bacterial culture was used for total DNA isolation using Genomic DNA Extraction kit (Real Biotech Corporation).

Recombinant:

Article Title: A novel medium-throughput biological assay system for HTLV-1 infectivity and drug discovery
Article Snippet: .. Once the selected colonies were grown in the Luria-Bertani broth (LB, Himedia) overnight, the recombinant vector was purified by the QIAGEN Plasmid Mini Kit (Qiagen, Germany). .. Cell lines and culture Baby hamster kidney cells (BHK-21), which has demonstrated high susceptibility to HTLV-1 (Cat No: C107) was obtained from the National Cell Bank of Iran (Pasteur Institute of Iran, Tehran).

Immunofluorescence:

Article Title: Phagocytosis of full-length Tau oligomers by Actin-remodeling of activated microglia
Article Snippet: Chemical and reagents Luria-Bertani broth (Himedia), ampicillin, NaCl, phenylmethylsulfonylfluoride (PMSF), MgCl2, ammonium persulfate (APS), heparin (17,500 Da), DMSO, and methanol were purchased from MP Biomedicals; IPTG and dithiothreitol (DTT) from Calbiochem; MES, BES, glutaraldehyde, and SDS from Sigma; and EGTA, protease inhibitor cocktail, Tris base, 40% acrylamide, and TEMED from Invitrogen. .. The coverslip of 0.17 mm for immunofluorescence study and copper-coated carbon grids for TEM study were purchased from Blue star and Ted Pella, Inc., respectively.

Transmission Electron Microscopy:

Article Title: Prevalence and antibacterial resistance patterns of extended-spectrum beta-lactamase producing Gram-negative bacteria isolated from ocular infections
Article Snippet: Preparation of template DNA from isolated Gram-negative bacteria A single colony of each nonduplicate Gram-negative isolate was inoculated from MacConkey agar into 5 ml of Luria-Bertani broth (M1245; Hi-Media Laboratories Pvt. .. Along with the test isolates, DNA of bla CTX-M -gene carrying E. coli 4712 (positive control for bla CTX-M-15 ; GenBank accession number KC200080), DNA of bla SHV -gene carrying K. pneumoniae (ATCC 700603) (positive control for bla SHV -gene),[ ] DNA of bla TEM -gene carrying E. coli (ATCC 35218) (positive control for bla TEM -gene),[ ] DNA of bla OXA -gene laboratory isolate of K. pneumoniae 7888 (positive control for bla OXA -gene GenBank accession number JN565742), and DNA of non-ESBL-producing organism, E. coli ATCC 25922 were also extracted for performing positive and negative control during each mPCR.

Article Title: Phagocytosis of full-length Tau oligomers by Actin-remodeling of activated microglia
Article Snippet: Chemical and reagents Luria-Bertani broth (Himedia), ampicillin, NaCl, phenylmethylsulfonylfluoride (PMSF), MgCl2, ammonium persulfate (APS), heparin (17,500 Da), DMSO, and methanol were purchased from MP Biomedicals; IPTG and dithiothreitol (DTT) from Calbiochem; MES, BES, glutaraldehyde, and SDS from Sigma; and EGTA, protease inhibitor cocktail, Tris base, 40% acrylamide, and TEMED from Invitrogen. .. The coverslip of 0.17 mm for immunofluorescence study and copper-coated carbon grids for TEM study were purchased from Blue star and Ted Pella, Inc., respectively.

DNA Extraction:

Article Title: Bioremediation of Agro-Based Pulp Mill Effluent by Microbial Consortium Comprising Autochthonous Bacteria
Article Snippet: For 16 S rRNA sequencing, the bacterial culture was inoculated in Luria Bertani broth (Hi-Media). .. Overnight grown bacterial culture was used for total DNA isolation using Genomic DNA Extraction kit (Real Biotech Corporation).

Isolation:

Article Title: Prevalence and antibacterial resistance patterns of extended-spectrum beta-lactamase producing Gram-negative bacteria isolated from ocular infections
Article Snippet: .. Preparation of template DNA from isolated Gram-negative bacteria A single colony of each nonduplicate Gram-negative isolate was inoculated from MacConkey agar into 5 ml of Luria-Bertani broth (M1245; Hi-Media Laboratories Pvt. .. Ltd., Mumbai, India) and incubated for 20 h at 37°C.

Article Title: Anti-virulence potential of eugenol-rich fraction of Syzygium aromaticum against multidrug resistant uropathogens isolated from catheterized patients
Article Snippet: Bacterial strains and culture conditions The bacterial strains used in this study were P. aeruginosa PAO1 (MTCC 3541) and 2 clinical isolates - P. aeruginosa RRLP1 (GenBank ID: ) and P. aeruginosa RRLP2 (GenBank ID: ), isolated from patients having CAUTIs, referring to Government Vellore Medical College and Hospital, Adukkamparai, India. .. All the test organisms were cultured in Luria-Bertani broth (Himedia Laboratories Pvt Ltd, Bangalore, India, pH 7.0) and maintained under 120 rpm at 37 °C.

Article Title: Antibiofilm Activity of Biosurfactant Producing Coral Associated Bacteria Isolated from Gulf of Mannar
Article Snippet: CAB used in this study were isolated earlier by our group from the mucus of the coral Acropora digitifera collected from Gulf of Mannar (9º12′N, 79º5′E) and maintained in glycerol stocks at −86 °C. .. Pseudomonas aeruginosa ATCC10145 used for antibiofilm assays was maintained in Luria–Bertani broth (HiMedia Laboratories, Mumbai) at 37 °C.

Size-exclusion Chromatography:

Article Title: Emergence of integron borne PER-1 mediated extended spectrum cephalosporin resistance among nosocomial isolates of Gram-negative bacilli
Article Snippet: .. PCR reactions were run under the following conditions: initial denaturation at 95°C for two min, 30 cycles of 95 °C for 20 sec, 52°C for 45 sec, 72°C for one min and final extension at 72°C for five min. Plasmid preparation : All ESBL positive bacterial isolates were cultured in Luria-Bertani broth (Hi-Media, Mumbai, India) containing 1µg/ml of cefotaxime. .. Cultures were incubated on shaker incubator overnight at 37°C.

Purification:

Article Title: Emergence of integron borne PER-1 mediated extended spectrum cephalosporin resistance among nosocomial isolates of Gram-negative bacilli
Article Snippet: PCR reactions were run under the following conditions: initial denaturation at 95°C for two min, 30 cycles of 95 °C for 20 sec, 52°C for 45 sec, 72°C for one min and final extension at 72°C for five min. Plasmid preparation : All ESBL positive bacterial isolates were cultured in Luria-Bertani broth (Hi-Media, Mumbai, India) containing 1µg/ml of cefotaxime. .. Plasmids were purified by Gene Jet plasmid Miniprep kit (Thermoscientific, Lithuania).

Article Title: A novel medium-throughput biological assay system for HTLV-1 infectivity and drug discovery
Article Snippet: .. Once the selected colonies were grown in the Luria-Bertani broth (LB, Himedia) overnight, the recombinant vector was purified by the QIAGEN Plasmid Mini Kit (Qiagen, Germany). .. Cell lines and culture Baby hamster kidney cells (BHK-21), which has demonstrated high susceptibility to HTLV-1 (Cat No: C107) was obtained from the National Cell Bank of Iran (Pasteur Institute of Iran, Tehran).

Polymerase Chain Reaction:

Article Title: Emergence of integron borne PER-1 mediated extended spectrum cephalosporin resistance among nosocomial isolates of Gram-negative bacilli
Article Snippet: .. PCR reactions were run under the following conditions: initial denaturation at 95°C for two min, 30 cycles of 95 °C for 20 sec, 52°C for 45 sec, 72°C for one min and final extension at 72°C for five min. Plasmid preparation : All ESBL positive bacterial isolates were cultured in Luria-Bertani broth (Hi-Media, Mumbai, India) containing 1µg/ml of cefotaxime. .. Cultures were incubated on shaker incubator overnight at 37°C.

Article Title: Bioremediation of Agro-Based Pulp Mill Effluent by Microbial Consortium Comprising Autochthonous Bacteria
Article Snippet: For 16 S rRNA sequencing, the bacterial culture was inoculated in Luria Bertani broth (Hi-Media). .. The PCR reaction mixture contained and assay buffer 5 μ L, forward primer 1 microliter, reverse primer 1 microliter, dNTP 1 μ L, template 2 μ L, tag polymerase 1 μ L, and final total volume was made up 50 μ L with milli Q. Polymerase chain reaction was performed in a thermo cycler (BIORAD) under the following conditions, denaturation at 94°C for 1 min, followed by annealing at 55°C for 1 min, and extension at 72°C for 2 min, for 35 repeated cycles.

Article Title: A novel medium-throughput biological assay system for HTLV-1 infectivity and drug discovery
Article Snippet: Firstly, SV40 promoter from pCDNA3.1Hyg+ and fragment of pEGFP-N1 containing the EGFP were amplified by PCR using the following set of primers: SV40 FWD =5’ CAAGATCTGGTTAAAAAATGAGCTGA-TTTA 3’, SV40 Rev =5’TTGCTCACCATCACGT GCTGATCA-GAT 3’. .. Once the selected colonies were grown in the Luria-Bertani broth (LB, Himedia) overnight, the recombinant vector was purified by the QIAGEN Plasmid Mini Kit (Qiagen, Germany).

Antibiofilm Assay:

Article Title: Curcumin Quantum Dots Mediated Degradation of Bacterial Biofilms
Article Snippet: Antibiofilm Activity Determination Using Tissue Culture Plate Assay (TCP) The antibiofilm assay was performed in 96-well tissue culture plate as described previously with minor modifications ( ; ). .. Briefly, overnight cultures of staphylococcal strains [S. aureus (ATCC 29213), S. epidermidis (ATCC 35984 and ATCC 35983), and MRSA were diluted 1:100 in 50% brain heart infusion (BHI) broth (HiMedia laboratories, India)/glucose (4%) at pH 6.6, whereas, E. coli (ATCC 25922) and P. aeruginosa (ATCC 25619) were grown in Luria Bertani broth (HiMedia laboratories, India)].

Activated Clotting Time Assay:

Article Title: Emergence of integron borne PER-1 mediated extended spectrum cephalosporin resistance among nosocomial isolates of Gram-negative bacilli
Article Snippet: Characterization of integrons by integrase gene PCR : For characterization of class 1 and class 2 integrons, integrase gene PCR assays were performed by using primers Int1F (CAG TGG ACA TAA GCC TGT TC), Int1R (CCC GAG GCA TAG ACT GTA) and Int2F (TTG CGA GTA TCC ATA ACC TG), Int2R (TTA CCT GCA CTG GAT TAA GC) . .. PCR reactions were run under the following conditions: initial denaturation at 95°C for two min, 30 cycles of 95 °C for 20 sec, 52°C for 45 sec, 72°C for one min and final extension at 72°C for five min. Plasmid preparation : All ESBL positive bacterial isolates were cultured in Luria-Bertani broth (Hi-Media, Mumbai, India) containing 1µg/ml of cefotaxime.

Plasmid Preparation:

Article Title: Genetic Acquisition of NDM Gene Offers Sustainability among Clinical Isolates of Pseudomonas aeruginosa in Clinical Settings
Article Snippet: Paragraph title: Transferability & plasmid profiling ... Overnight culture of bacteria were diluted in Luria Bertani broth (Hi-Media, Mumbai, India) and was grown at 37°C till the O.D. of the recipient and donor culture reached 0.8–0.9 at A600 .

Article Title: Emergence of integron borne PER-1 mediated extended spectrum cephalosporin resistance among nosocomial isolates of Gram-negative bacilli
Article Snippet: .. PCR reactions were run under the following conditions: initial denaturation at 95°C for two min, 30 cycles of 95 °C for 20 sec, 52°C for 45 sec, 72°C for one min and final extension at 72°C for five min. Plasmid preparation : All ESBL positive bacterial isolates were cultured in Luria-Bertani broth (Hi-Media, Mumbai, India) containing 1µg/ml of cefotaxime. .. Cultures were incubated on shaker incubator overnight at 37°C.

Article Title: A novel medium-throughput biological assay system for HTLV-1 infectivity and drug discovery
Article Snippet: .. Once the selected colonies were grown in the Luria-Bertani broth (LB, Himedia) overnight, the recombinant vector was purified by the QIAGEN Plasmid Mini Kit (Qiagen, Germany). .. Cell lines and culture Baby hamster kidney cells (BHK-21), which has demonstrated high susceptibility to HTLV-1 (Cat No: C107) was obtained from the National Cell Bank of Iran (Pasteur Institute of Iran, Tehran).

Software:

Article Title: A comparative study of coastal and clinical isolates ofPseudomonas aeruginosa
Article Snippet: Screening of proteolytic and haemolytic activities To determine the proteolytic and haemolytic activities, 5 μL of overnight cultures grown in Luria-Bertani broth (Himedia, India) were spotted on casein milk agar and 5% blood agar plates, respectively ( ). .. The isolates were grouped using UPGMA cluster analysis, and their phenotypic similarities were calculated with the Bray-Curtis similarity coefficient using the PRIMER-5 software ( ).

Negative Control:

Article Title: Prevalence and antibacterial resistance patterns of extended-spectrum beta-lactamase producing Gram-negative bacteria isolated from ocular infections
Article Snippet: Preparation of template DNA from isolated Gram-negative bacteria A single colony of each nonduplicate Gram-negative isolate was inoculated from MacConkey agar into 5 ml of Luria-Bertani broth (M1245; Hi-Media Laboratories Pvt. .. Along with the test isolates, DNA of bla CTX-M -gene carrying E. coli 4712 (positive control for bla CTX-M-15 ; GenBank accession number KC200080), DNA of bla SHV -gene carrying K. pneumoniae (ATCC 700603) (positive control for bla SHV -gene),[ ] DNA of bla TEM -gene carrying E. coli (ATCC 35218) (positive control for bla TEM -gene),[ ] DNA of bla OXA -gene laboratory isolate of K. pneumoniae 7888 (positive control for bla OXA -gene GenBank accession number JN565742), and DNA of non-ESBL-producing organism, E. coli ATCC 25922 were also extracted for performing positive and negative control during each mPCR.

Concentration Assay:

Article Title: Multidrug-Resistance and Extended Spectrum Beta-Lactamase Production in Uropathogenic E. Coli which were Isolated from Hospitalized Patients in Kolkata, India
Article Snippet: A significant monomicrobic bacteriuria was defined as the culture of a single bacterial species from the urine sample at a concentration of > 105 cfus/ml. .. The media which were used in the study included, MacConkey’s Agar, Eosin Methylene Blue Agar and Luria Bertani Broth (Hi-Media, Mumbai).The cultures were incubated at 37ºC for 24 hrs respectively.

Alkaline Lysis:

Article Title: Prevalence and antibacterial resistance patterns of extended-spectrum beta-lactamase producing Gram-negative bacteria isolated from ocular infections
Article Snippet: Preparation of template DNA from isolated Gram-negative bacteria A single colony of each nonduplicate Gram-negative isolate was inoculated from MacConkey agar into 5 ml of Luria-Bertani broth (M1245; Hi-Media Laboratories Pvt. .. The DNA was extracted from each bacterial isolate by alkaline lysis method using QIAamp DNA Mini Kit (Code No. 51304) according to the manufacturer's instruction (Qiagen Gmbh, Hilden, Germany).

CTG Assay:

Article Title: Emergence of integron borne PER-1 mediated extended spectrum cephalosporin resistance among nosocomial isolates of Gram-negative bacilli
Article Snippet: Characterization of integrons by integrase gene PCR : For characterization of class 1 and class 2 integrons, integrase gene PCR assays were performed by using primers Int1F (CAG TGG ACA TAA GCC TGT TC), Int1R (CCC GAG GCA TAG ACT GTA) and Int2F (TTG CGA GTA TCC ATA ACC TG), Int2R (TTA CCT GCA CTG GAT TAA GC) . .. PCR reactions were run under the following conditions: initial denaturation at 95°C for two min, 30 cycles of 95 °C for 20 sec, 52°C for 45 sec, 72°C for one min and final extension at 72°C for five min. Plasmid preparation : All ESBL positive bacterial isolates were cultured in Luria-Bertani broth (Hi-Media, Mumbai, India) containing 1µg/ml of cefotaxime.

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    HiMedia Laboratories luria bertani broth
    Luria Bertani Broth, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luria bertani broth/product/HiMedia Laboratories
    Average 95 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    luria bertani broth - by Bioz Stars, 2020-04
    95/100 stars
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