luria bertani bacterial media  (Millipore)


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    Structured Review

    Millipore luria bertani bacterial media
    Luria Bertani Bacterial Media, supplied by Millipore, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luria bertani bacterial media/product/Millipore
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    luria bertani bacterial media - by Bioz Stars, 2020-04
    87/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Monitoring Lipid Anchor Organization in Cell Membranes by PIE-FCCS
    Article Snippet: Expression was induced during log phase growth with 1 mM isopropyl β-d -1-thiolgalatopyranoside (IPTG, Sigma) in 1 L suspension of Luria–Bertani bacterial media (Sigma) at 37 °C for 3–5 h. Cells were lysed by a freeze–thaw cycle, conventional treatment with 1 mg/mL lysozyme (Sigma) for 1 h at 4 °C in lysis buffer (40 mM Tris pH 7.4, 275 mM NaCl, 20 mM imidazole, 2% protease inhibitor cocktail for His-tag (Sigma)), and then by probe sonicator (Sonics & Materials Inc., VCX750). .. Lysate was clarified by addition of, and incubation with, nucleases (100 ng/mL RNaseA (Roche) and 25 ng/mL DNaseI (Roche)) and high-speed centrifugation (6000 rcf) for 45 min at 4 °C and then filtered through a 0.45 μm syringe filter.

    Affinity Chromatography:

    Article Title: Monitoring Lipid Anchor Organization in Cell Membranes by PIE-FCCS
    Article Snippet: Expression was induced during log phase growth with 1 mM isopropyl β-d -1-thiolgalatopyranoside (IPTG, Sigma) in 1 L suspension of Luria–Bertani bacterial media (Sigma) at 37 °C for 3–5 h. Cells were lysed by a freeze–thaw cycle, conventional treatment with 1 mg/mL lysozyme (Sigma) for 1 h at 4 °C in lysis buffer (40 mM Tris pH 7.4, 275 mM NaCl, 20 mM imidazole, 2% protease inhibitor cocktail for His-tag (Sigma)), and then by probe sonicator (Sonics & Materials Inc., VCX750). .. His-tagged proteins were purified by immobilized nickel affinity chromatography in a 1 mL His-Trap column on an AKTA Explorer (GE Life Sciences) and by gel filtration chromatography on a Superdex-100 HR size exclusion column (GE Life Sciences) in phosphate-buffered saline, pH 7.4 (PBS, Gibco, Cellgro), and 20% glycerol (EMD).

    Filtration:

    Article Title: Monitoring Lipid Anchor Organization in Cell Membranes by PIE-FCCS
    Article Snippet: Expression was induced during log phase growth with 1 mM isopropyl β-d -1-thiolgalatopyranoside (IPTG, Sigma) in 1 L suspension of Luria–Bertani bacterial media (Sigma) at 37 °C for 3–5 h. Cells were lysed by a freeze–thaw cycle, conventional treatment with 1 mg/mL lysozyme (Sigma) for 1 h at 4 °C in lysis buffer (40 mM Tris pH 7.4, 275 mM NaCl, 20 mM imidazole, 2% protease inhibitor cocktail for His-tag (Sigma)), and then by probe sonicator (Sonics & Materials Inc., VCX750). .. His-tagged proteins were purified by immobilized nickel affinity chromatography in a 1 mL His-Trap column on an AKTA Explorer (GE Life Sciences) and by gel filtration chromatography on a Superdex-100 HR size exclusion column (GE Life Sciences) in phosphate-buffered saline, pH 7.4 (PBS, Gibco, Cellgro), and 20% glycerol (EMD).

    Chromatography:

    Article Title: Monitoring Lipid Anchor Organization in Cell Membranes by PIE-FCCS
    Article Snippet: Expression was induced during log phase growth with 1 mM isopropyl β-d -1-thiolgalatopyranoside (IPTG, Sigma) in 1 L suspension of Luria–Bertani bacterial media (Sigma) at 37 °C for 3–5 h. Cells were lysed by a freeze–thaw cycle, conventional treatment with 1 mg/mL lysozyme (Sigma) for 1 h at 4 °C in lysis buffer (40 mM Tris pH 7.4, 275 mM NaCl, 20 mM imidazole, 2% protease inhibitor cocktail for His-tag (Sigma)), and then by probe sonicator (Sonics & Materials Inc., VCX750). .. His-tagged proteins were purified by immobilized nickel affinity chromatography in a 1 mL His-Trap column on an AKTA Explorer (GE Life Sciences) and by gel filtration chromatography on a Superdex-100 HR size exclusion column (GE Life Sciences) in phosphate-buffered saline, pH 7.4 (PBS, Gibco, Cellgro), and 20% glycerol (EMD).

    Protease Inhibitor:

    Article Title: Monitoring Lipid Anchor Organization in Cell Membranes by PIE-FCCS
    Article Snippet: .. Expression was induced during log phase growth with 1 mM isopropyl β-d -1-thiolgalatopyranoside (IPTG, Sigma) in 1 L suspension of Luria–Bertani bacterial media (Sigma) at 37 °C for 3–5 h. Cells were lysed by a freeze–thaw cycle, conventional treatment with 1 mg/mL lysozyme (Sigma) for 1 h at 4 °C in lysis buffer (40 mM Tris pH 7.4, 275 mM NaCl, 20 mM imidazole, 2% protease inhibitor cocktail for His-tag (Sigma)), and then by probe sonicator (Sonics & Materials Inc., VCX750). .. Samples were on ice during pulse sonication (5 s ON/9 s OFF, 150 s, amplitude = 35%, with a 3 mm stepped microtip).

    Purification:

    Article Title: Monitoring Lipid Anchor Organization in Cell Membranes by PIE-FCCS
    Article Snippet: Paragraph title: Protein Expression and Purification ... Expression was induced during log phase growth with 1 mM isopropyl β-d -1-thiolgalatopyranoside (IPTG, Sigma) in 1 L suspension of Luria–Bertani bacterial media (Sigma) at 37 °C for 3–5 h. Cells were lysed by a freeze–thaw cycle, conventional treatment with 1 mg/mL lysozyme (Sigma) for 1 h at 4 °C in lysis buffer (40 mM Tris pH 7.4, 275 mM NaCl, 20 mM imidazole, 2% protease inhibitor cocktail for His-tag (Sigma)), and then by probe sonicator (Sonics & Materials Inc., VCX750).

    Incubation:

    Article Title: Monitoring Lipid Anchor Organization in Cell Membranes by PIE-FCCS
    Article Snippet: Expression was induced during log phase growth with 1 mM isopropyl β-d -1-thiolgalatopyranoside (IPTG, Sigma) in 1 L suspension of Luria–Bertani bacterial media (Sigma) at 37 °C for 3–5 h. Cells were lysed by a freeze–thaw cycle, conventional treatment with 1 mg/mL lysozyme (Sigma) for 1 h at 4 °C in lysis buffer (40 mM Tris pH 7.4, 275 mM NaCl, 20 mM imidazole, 2% protease inhibitor cocktail for His-tag (Sigma)), and then by probe sonicator (Sonics & Materials Inc., VCX750). .. Lysate was clarified by addition of, and incubation with, nucleases (100 ng/mL RNaseA (Roche) and 25 ng/mL DNaseI (Roche)) and high-speed centrifugation (6000 rcf) for 45 min at 4 °C and then filtered through a 0.45 μm syringe filter.

    Expressing:

    Article Title: Monitoring Lipid Anchor Organization in Cell Membranes by PIE-FCCS
    Article Snippet: .. Expression was induced during log phase growth with 1 mM isopropyl β-d -1-thiolgalatopyranoside (IPTG, Sigma) in 1 L suspension of Luria–Bertani bacterial media (Sigma) at 37 °C for 3–5 h. Cells were lysed by a freeze–thaw cycle, conventional treatment with 1 mg/mL lysozyme (Sigma) for 1 h at 4 °C in lysis buffer (40 mM Tris pH 7.4, 275 mM NaCl, 20 mM imidazole, 2% protease inhibitor cocktail for His-tag (Sigma)), and then by probe sonicator (Sonics & Materials Inc., VCX750). .. Samples were on ice during pulse sonication (5 s ON/9 s OFF, 150 s, amplitude = 35%, with a 3 mm stepped microtip).

    Lysis:

    Article Title: Monitoring Lipid Anchor Organization in Cell Membranes by PIE-FCCS
    Article Snippet: .. Expression was induced during log phase growth with 1 mM isopropyl β-d -1-thiolgalatopyranoside (IPTG, Sigma) in 1 L suspension of Luria–Bertani bacterial media (Sigma) at 37 °C for 3–5 h. Cells were lysed by a freeze–thaw cycle, conventional treatment with 1 mg/mL lysozyme (Sigma) for 1 h at 4 °C in lysis buffer (40 mM Tris pH 7.4, 275 mM NaCl, 20 mM imidazole, 2% protease inhibitor cocktail for His-tag (Sigma)), and then by probe sonicator (Sonics & Materials Inc., VCX750). .. Samples were on ice during pulse sonication (5 s ON/9 s OFF, 150 s, amplitude = 35%, with a 3 mm stepped microtip).

    Sonication:

    Article Title: Monitoring Lipid Anchor Organization in Cell Membranes by PIE-FCCS
    Article Snippet: Expression was induced during log phase growth with 1 mM isopropyl β-d -1-thiolgalatopyranoside (IPTG, Sigma) in 1 L suspension of Luria–Bertani bacterial media (Sigma) at 37 °C for 3–5 h. Cells were lysed by a freeze–thaw cycle, conventional treatment with 1 mg/mL lysozyme (Sigma) for 1 h at 4 °C in lysis buffer (40 mM Tris pH 7.4, 275 mM NaCl, 20 mM imidazole, 2% protease inhibitor cocktail for His-tag (Sigma)), and then by probe sonicator (Sonics & Materials Inc., VCX750). .. Samples were on ice during pulse sonication (5 s ON/9 s OFF, 150 s, amplitude = 35%, with a 3 mm stepped microtip).

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    Millipore luria bertani media
    Luria Bertani Media, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luria bertani media/product/Millipore
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    luria bertani media - by Bioz Stars, 2020-04
    94/100 stars
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