luminol based immobilon western chemiluminescence detection kit  (Millipore)


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    Name:
    Luminol
    Description:
    Luminol is a chemiluminescent compound which belongs to the class of cyclic acyl hydrazides It exhibits chemiluminescence when oxidized by oxidizing agents Luminol may be associated with nausea vomiting and mucosa irritation in eyes and skin
    Catalog Number:
    a8511
    Price:
    None
    Applications:
    Luminol has been used in chemiluminescence assay.
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    Structured Review

    Millipore luminol based immobilon western chemiluminescence detection kit
    Luminol
    Luminol is a chemiluminescent compound which belongs to the class of cyclic acyl hydrazides It exhibits chemiluminescence when oxidized by oxidizing agents Luminol may be associated with nausea vomiting and mucosa irritation in eyes and skin
    https://www.bioz.com/result/luminol based immobilon western chemiluminescence detection kit/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    luminol based immobilon western chemiluminescence detection kit - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Blood clotting and traumatic injury with shock mediates complement‐dependent neutrophil priming for extracellular ROS, ROS‐dependent organ injury and coagulopathy
    Article Snippet: .. After a 5‐min incubation at 37o C with the designated luminol solution, either vehicle control or N‐formyl‐methionyl‐leucyl‐phenylalanine (fMLP, final assay concentration 100 nM; Sigma) were added and chemiluminescence events were measured over 10 min. Quantification of ROS was performed by integrating the area under the curve of chemiluminescence events over time, as described previously . .. For experiments using healthy volunteers to measure the effects of different blood products on PMN priming, PMN were used with autologous blood products, and an internal positive priming control with the well‐known priming agent tumour necrosis factor (TNF)‐ɑ (20 ng/ml; R & D Systems, Minneapolis, MN, USA) was used to set the reference of normalization, such that comparison of priming was feasible across multiple different types of clotted and unclotted blood products.

    Incubation:

    Article Title: Macrophages from elders are more permissive to intracellular multiplication of Mycobacterium tuberculosis
    Article Snippet: .. Either 5 × 105 bacteria (MOI = 5) or 150 nM 12- O -tetradecanoylphorbol-13-acetate (TPA, Calbiochem), luminol 500 μM (Sigma-Aldrich Spain) and Hanks balanced salt solution were added to 105 monocytes in a total volume of 100 μL, and incubated for 30 min at 37 °C. .. After 30 min, emitted light was measured for 5 sec at 21 °C in a TopCount-NXT microplate scintillation and luminescence counter (Packard, Perkin Elmer España, Madrid, Spain), and ROS formation was expressed as counted photons per second (cps).

    Article Title: Sulphamethazine derivatives as immunomodulating agents: New therapeutic strategies for inflammatory diseases
    Article Snippet: .. After incubation, 25 μL of serum opsonized zymosan (SOZ) [Fluka, Buchs, Switzerland] and 25 μL of intracellular reactive oxygen species detecting probe, luminol [Research Organics, Cleveland, OH, USA] were added into each well, except blank wells (containing only HBSS++ ). ..

    Article Title: Blood clotting and traumatic injury with shock mediates complement‐dependent neutrophil priming for extracellular ROS, ROS‐dependent organ injury and coagulopathy
    Article Snippet: .. After a 5‐min incubation at 37o C with the designated luminol solution, either vehicle control or N‐formyl‐methionyl‐leucyl‐phenylalanine (fMLP, final assay concentration 100 nM; Sigma) were added and chemiluminescence events were measured over 10 min. Quantification of ROS was performed by integrating the area under the curve of chemiluminescence events over time, as described previously . .. For experiments using healthy volunteers to measure the effects of different blood products on PMN priming, PMN were used with autologous blood products, and an internal positive priming control with the well‐known priming agent tumour necrosis factor (TNF)‐ɑ (20 ng/ml; R & D Systems, Minneapolis, MN, USA) was used to set the reference of normalization, such that comparison of priming was feasible across multiple different types of clotted and unclotted blood products.

    Activity Assay:

    Article Title: NADPH Oxidase-Derived Superoxide Provides a Third Signal for CD4 T cell Effector Responses
    Article Snippet: .. The redox-sensitive and cell permeable molecule luminol (Sigma) was utilized to assess NOX activity within OT-II and OT-II. ..

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  • 99
    Millipore luminol based immobilon western chemiluminescence detection kit
    Luminol Based Immobilon Western Chemiluminescence Detection Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luminol based immobilon western chemiluminescence detection kit/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    luminol based immobilon western chemiluminescence detection kit - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

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