luciferase reporter plasmid pnifty2 luc  (InvivoGen)

 
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    Name:
    pNiFty2 Luc
    Description:
    pNiFty2 NF κB 5x ELAM ZeoR Luc
    Catalog Number:
    pnifty2-luc
    Price:
    None
    Size:
    20 µg
    Category:
    pNiFty2 Luc pNiFty2 NF κB inducible plasmids TLR Detection Toll Like Receptors TLRs Innate Immunity Sensors Research Fields
    Buy from Supplier


    Structured Review

    InvivoGen luciferase reporter plasmid pnifty2 luc
    pNiFty2 NF κB 5x ELAM ZeoR Luc
    https://www.bioz.com/result/luciferase reporter plasmid pnifty2 luc/product/InvivoGen
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    luciferase reporter plasmid pnifty2 luc - by Bioz Stars, 2020-09
    94/100 stars

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    Related Articles

    Transfection:

    Article Title: Inhibition of Toll-like receptor signaling in primary murine microglia
    Article Snippet: .. Although the transfection efficiency using nucleofection was still low ( < 10%), luciferase expression occured only in cells that took up the pNiFty2-Luc plasmid. .. Following nucleofection, the cells were plated in 12-well plates and incubated overnight at 37°C.

    Article Title: Synthesis of a Highly Water-Soluble Derivative of Amphotericin B with Attenuated Proinflammatory Activity
    Article Snippet: .. Human embryonic kidney 293 (HEK-293) cells stably transfected (individually) with either human TLR2, or TLR4 were obtained from InvivoGen, Inc. (San Diego, CA) and were additionally nucleofected with 1 μg of purified pnifty2 reporter plasmid (InvivoGen) using an Amaxa Nucleofector (Gaithersburg, MD). .. The pnifty2 reporter plasmid expresses a secreted embryonic alkaline phosphatase (SEAP) gene under the control of a promoter inducible by NF-κB and AP-1 transcription factors.

    Article Title: Yeast Virus-Derived Stimulator of the Innate Immune System Augments the Efficacy of Virus Vector-Based Immunotherapy
    Article Snippet: .. All hTLR cell lines were stably transfected with the NF-κB-inducible reporter plasmid pNiFty2-luc (InvivoGen) encoding the firefly luciferase gene under the control of an engineered ELAM1 promoter which combines five NF-κB sites and the proximal ELAM promoter, as well as a zeocin resistance gene. .. Emerging clones, named HEK-hTLR-luc, were tested for luciferase activity after stimulation with the respective TLR-specific ligands.

    Article Title: A Dairy Bacterium Displays InVitro Probiotic Properties for the Pharyngeal Mucosa by Antagonizing Group A Streptococci and Modulating the Immune Response ▿
    Article Snippet: .. The FaDu cell line was stably transfected with the luciferase reporter plasmid pNiFty2-Luc (InvivoGen, LaboGen, Rho, Italy), as described previously ( ). .. In pNiFty2-Luc, the expression of an insect luciferase gene is induced by the active NF-κB molecules present in the cell.

    Article Title: In Vitro Functional and Immunomodulatory Properties of the Lactobacillus helveticus MIMLh5- Streptococcus salivarius ST3 Association That Are Relevant to the Development of a Pharyngeal Probiotic Product
    Article Snippet: .. Stably transfected FaDu cell lines were generated by transfecting cells with the plasmid pNiFty2-Luc (Invivogen, Labogen, Rho, Italy) as described by Guglielmetti et al. ( ). .. This plasmid contains a promoter combining five nuclear factor-κB (NF-κB)-binding sites and the luc firefly luciferase reporter gene.

    Article Title: Inhibition of Toll-like receptor signaling in primary murine microglia
    Article Snippet: .. In order to deduce the activation levels of NF-κB in these cells during HSV infection, 1 × 106 microglial cells from both wild type and TLR2 KO mice were transfected with pNiFty2-Luc plasmid and, after overnight incubation, they were infected with HSV at a MOI of 2. .. At 5 h p.i., activation of NF-κB was measured using a luciferase reporter gene assay.

    Luciferase:

    Article Title: Inhibition of Toll-like receptor signaling in primary murine microglia
    Article Snippet: .. Although the transfection efficiency using nucleofection was still low ( < 10%), luciferase expression occured only in cells that took up the pNiFty2-Luc plasmid. .. Following nucleofection, the cells were plated in 12-well plates and incubated overnight at 37°C.

    Article Title: Yeast Virus-Derived Stimulator of the Innate Immune System Augments the Efficacy of Virus Vector-Based Immunotherapy
    Article Snippet: .. All hTLR cell lines were stably transfected with the NF-κB-inducible reporter plasmid pNiFty2-luc (InvivoGen) encoding the firefly luciferase gene under the control of an engineered ELAM1 promoter which combines five NF-κB sites and the proximal ELAM promoter, as well as a zeocin resistance gene. .. Emerging clones, named HEK-hTLR-luc, were tested for luciferase activity after stimulation with the respective TLR-specific ligands.

    Article Title: A Dairy Bacterium Displays InVitro Probiotic Properties for the Pharyngeal Mucosa by Antagonizing Group A Streptococci and Modulating the Immune Response ▿
    Article Snippet: .. The FaDu cell line was stably transfected with the luciferase reporter plasmid pNiFty2-Luc (InvivoGen, LaboGen, Rho, Italy), as described previously ( ). .. In pNiFty2-Luc, the expression of an insect luciferase gene is induced by the active NF-κB molecules present in the cell.

    Stable Transfection:

    Article Title: Synthesis of a Highly Water-Soluble Derivative of Amphotericin B with Attenuated Proinflammatory Activity
    Article Snippet: .. Human embryonic kidney 293 (HEK-293) cells stably transfected (individually) with either human TLR2, or TLR4 were obtained from InvivoGen, Inc. (San Diego, CA) and were additionally nucleofected with 1 μg of purified pnifty2 reporter plasmid (InvivoGen) using an Amaxa Nucleofector (Gaithersburg, MD). .. The pnifty2 reporter plasmid expresses a secreted embryonic alkaline phosphatase (SEAP) gene under the control of a promoter inducible by NF-κB and AP-1 transcription factors.

    Article Title: Yeast Virus-Derived Stimulator of the Innate Immune System Augments the Efficacy of Virus Vector-Based Immunotherapy
    Article Snippet: .. All hTLR cell lines were stably transfected with the NF-κB-inducible reporter plasmid pNiFty2-luc (InvivoGen) encoding the firefly luciferase gene under the control of an engineered ELAM1 promoter which combines five NF-κB sites and the proximal ELAM promoter, as well as a zeocin resistance gene. .. Emerging clones, named HEK-hTLR-luc, were tested for luciferase activity after stimulation with the respective TLR-specific ligands.

    Article Title: A Dairy Bacterium Displays InVitro Probiotic Properties for the Pharyngeal Mucosa by Antagonizing Group A Streptococci and Modulating the Immune Response ▿
    Article Snippet: .. The FaDu cell line was stably transfected with the luciferase reporter plasmid pNiFty2-Luc (InvivoGen, LaboGen, Rho, Italy), as described previously ( ). .. In pNiFty2-Luc, the expression of an insect luciferase gene is induced by the active NF-κB molecules present in the cell.

    Article Title: In Vitro Functional and Immunomodulatory Properties of the Lactobacillus helveticus MIMLh5- Streptococcus salivarius ST3 Association That Are Relevant to the Development of a Pharyngeal Probiotic Product
    Article Snippet: .. Stably transfected FaDu cell lines were generated by transfecting cells with the plasmid pNiFty2-Luc (Invivogen, Labogen, Rho, Italy) as described by Guglielmetti et al. ( ). .. This plasmid contains a promoter combining five nuclear factor-κB (NF-κB)-binding sites and the luc firefly luciferase reporter gene.

    Generated:

    Article Title: In Vitro Functional and Immunomodulatory Properties of the Lactobacillus helveticus MIMLh5- Streptococcus salivarius ST3 Association That Are Relevant to the Development of a Pharyngeal Probiotic Product
    Article Snippet: .. Stably transfected FaDu cell lines were generated by transfecting cells with the plasmid pNiFty2-Luc (Invivogen, Labogen, Rho, Italy) as described by Guglielmetti et al. ( ). .. This plasmid contains a promoter combining five nuclear factor-κB (NF-κB)-binding sites and the luc firefly luciferase reporter gene.

    Infection:

    Article Title: Inhibition of Toll-like receptor signaling in primary murine microglia
    Article Snippet: .. In order to deduce the activation levels of NF-κB in these cells during HSV infection, 1 × 106 microglial cells from both wild type and TLR2 KO mice were transfected with pNiFty2-Luc plasmid and, after overnight incubation, they were infected with HSV at a MOI of 2. .. At 5 h p.i., activation of NF-κB was measured using a luciferase reporter gene assay.

    Purification:

    Article Title: Synthesis of a Highly Water-Soluble Derivative of Amphotericin B with Attenuated Proinflammatory Activity
    Article Snippet: .. Human embryonic kidney 293 (HEK-293) cells stably transfected (individually) with either human TLR2, or TLR4 were obtained from InvivoGen, Inc. (San Diego, CA) and were additionally nucleofected with 1 μg of purified pnifty2 reporter plasmid (InvivoGen) using an Amaxa Nucleofector (Gaithersburg, MD). .. The pnifty2 reporter plasmid expresses a secreted embryonic alkaline phosphatase (SEAP) gene under the control of a promoter inducible by NF-κB and AP-1 transcription factors.

    Activation Assay:

    Article Title: Intracellular expression of toll-like receptor 4 in neuroblastoma cells and their unresponsiveness to lipopolysaccharide
    Article Snippet: .. To confirm the activation of NF-κB, we used another plasmid pNiFty2-Luc from Invivogen, San-Diego, CA, USA. ..

    Article Title: Inhibition of Toll-like receptor signaling in primary murine microglia
    Article Snippet: .. In order to deduce the activation levels of NF-κB in these cells during HSV infection, 1 × 106 microglial cells from both wild type and TLR2 KO mice were transfected with pNiFty2-Luc plasmid and, after overnight incubation, they were infected with HSV at a MOI of 2. .. At 5 h p.i., activation of NF-κB was measured using a luciferase reporter gene assay.

    Incubation:

    Article Title: Inhibition of Toll-like receptor signaling in primary murine microglia
    Article Snippet: .. In order to deduce the activation levels of NF-κB in these cells during HSV infection, 1 × 106 microglial cells from both wild type and TLR2 KO mice were transfected with pNiFty2-Luc plasmid and, after overnight incubation, they were infected with HSV at a MOI of 2. .. At 5 h p.i., activation of NF-κB was measured using a luciferase reporter gene assay.

    Expressing:

    Article Title: Inhibition of Toll-like receptor signaling in primary murine microglia
    Article Snippet: .. Although the transfection efficiency using nucleofection was still low ( < 10%), luciferase expression occured only in cells that took up the pNiFty2-Luc plasmid. .. Following nucleofection, the cells were plated in 12-well plates and incubated overnight at 37°C.

    Article Title: Inhibition of Toll-like receptor signaling in primary murine microglia
    Article Snippet: .. HEK293T cells, as well as wild-type and TLR2 KO microglia, were transfected with 1 μg pNiFty2-Luc plasmid (InvivoGen) expressing an NF-κB-driven firefly luciferase reporter gene. .. FuGene 6 was used for transfection of the 293T cells.

    Mouse Assay:

    Article Title: Inhibition of Toll-like receptor signaling in primary murine microglia
    Article Snippet: .. In order to deduce the activation levels of NF-κB in these cells during HSV infection, 1 × 106 microglial cells from both wild type and TLR2 KO mice were transfected with pNiFty2-Luc plasmid and, after overnight incubation, they were infected with HSV at a MOI of 2. .. At 5 h p.i., activation of NF-κB was measured using a luciferase reporter gene assay.

    Plasmid Preparation:

    Article Title: Intracellular expression of toll-like receptor 4 in neuroblastoma cells and their unresponsiveness to lipopolysaccharide
    Article Snippet: .. To confirm the activation of NF-κB, we used another plasmid pNiFty2-Luc from Invivogen, San-Diego, CA, USA. ..

    Article Title: Inhibition of Toll-like receptor signaling in primary murine microglia
    Article Snippet: .. Although the transfection efficiency using nucleofection was still low ( < 10%), luciferase expression occured only in cells that took up the pNiFty2-Luc plasmid. .. Following nucleofection, the cells were plated in 12-well plates and incubated overnight at 37°C.

    Article Title: Synthesis of a Highly Water-Soluble Derivative of Amphotericin B with Attenuated Proinflammatory Activity
    Article Snippet: .. Human embryonic kidney 293 (HEK-293) cells stably transfected (individually) with either human TLR2, or TLR4 were obtained from InvivoGen, Inc. (San Diego, CA) and were additionally nucleofected with 1 μg of purified pnifty2 reporter plasmid (InvivoGen) using an Amaxa Nucleofector (Gaithersburg, MD). .. The pnifty2 reporter plasmid expresses a secreted embryonic alkaline phosphatase (SEAP) gene under the control of a promoter inducible by NF-κB and AP-1 transcription factors.

    Article Title: Yeast Virus-Derived Stimulator of the Innate Immune System Augments the Efficacy of Virus Vector-Based Immunotherapy
    Article Snippet: .. All hTLR cell lines were stably transfected with the NF-κB-inducible reporter plasmid pNiFty2-luc (InvivoGen) encoding the firefly luciferase gene under the control of an engineered ELAM1 promoter which combines five NF-κB sites and the proximal ELAM promoter, as well as a zeocin resistance gene. .. Emerging clones, named HEK-hTLR-luc, were tested for luciferase activity after stimulation with the respective TLR-specific ligands.

    Article Title: A Dairy Bacterium Displays InVitro Probiotic Properties for the Pharyngeal Mucosa by Antagonizing Group A Streptococci and Modulating the Immune Response ▿
    Article Snippet: .. The FaDu cell line was stably transfected with the luciferase reporter plasmid pNiFty2-Luc (InvivoGen, LaboGen, Rho, Italy), as described previously ( ). .. In pNiFty2-Luc, the expression of an insect luciferase gene is induced by the active NF-κB molecules present in the cell.

    Article Title: In Vitro Functional and Immunomodulatory Properties of the Lactobacillus helveticus MIMLh5- Streptococcus salivarius ST3 Association That Are Relevant to the Development of a Pharyngeal Probiotic Product
    Article Snippet: .. Stably transfected FaDu cell lines were generated by transfecting cells with the plasmid pNiFty2-Luc (Invivogen, Labogen, Rho, Italy) as described by Guglielmetti et al. ( ). .. This plasmid contains a promoter combining five nuclear factor-κB (NF-κB)-binding sites and the luc firefly luciferase reporter gene.

    Article Title: Inhibition of Toll-like receptor signaling in primary murine microglia
    Article Snippet: .. In order to deduce the activation levels of NF-κB in these cells during HSV infection, 1 × 106 microglial cells from both wild type and TLR2 KO mice were transfected with pNiFty2-Luc plasmid and, after overnight incubation, they were infected with HSV at a MOI of 2. .. At 5 h p.i., activation of NF-κB was measured using a luciferase reporter gene assay.

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  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
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  • 94
    InvivoGen luciferase reporter plasmid pnifty2 luc
    Luciferase Reporter Plasmid Pnifty2 Luc, supplied by InvivoGen, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luciferase reporter plasmid pnifty2 luc/product/InvivoGen
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    luciferase reporter plasmid pnifty2 luc - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

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