luciferase reporter activities  (Promega)

 
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    Name:
    Luciferase Reporter Vectors and Cell Lines
    Description:
    Pre built NanoLuc firefly and Renilla reporter constructs for studying signaling pathways and creating NanoLuc fusion proteins
    Catalog Number:
    lrvacl-703186
    Price:
    None
    Category:
    Cell Biology Reporter Assays Transfection Reporter Vectors and Cell Lines
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    Structured Review

    Promega luciferase reporter activities
    Pre built NanoLuc firefly and Renilla reporter constructs for studying signaling pathways and creating NanoLuc fusion proteins
    https://www.bioz.com/result/luciferase reporter activities/product/Promega
    Average 99 stars, based on 44 article reviews
    Price from $9.99 to $1999.99
    luciferase reporter activities - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: MiR-138 induces cell cycle arrest by targeting cyclin D3 in hepatocellular carcinoma
    Article Snippet: .. The PCR product was cloned into Firefly luciferase reporter vector pGL3 (Promega Corporation, Madison, WI), termed as pGL3-CCND3-3′UTR. .. The plasmid which carried the mutated sequence in the two complementary sites for the seed region of miR-138 ( ) was generated based on pGL3-CCND3-3′UTR plasmid by MutanBEST Kit (Takara Bio, Shiga, JP), termed as pGL3-CCND3-3′UTR-mut.

    Transfection:

    Article Title: ZNF521 sustains the differentiation block in MLL-rearranged acute myeloid leukemia
    Article Snippet: .. For luciferase assay, 293T cells were cotransfected with 0.5 μg of the reporter plasmid, 1 μg of expression plasmid or empty vector and 0.5 μg of Renilla luciferase reporter vector (Promega) as internal control for normalization of transfection efficiency, for a total of 2 μg of combined plasmids per well. .. The cells were then harvested at 48 hours after transfection using a Dual-Luciferase reporter assay system (Promega) and the Victor3 TM 1420 Multilabel Counter (PerkinElmer).

    Article Title: Enhanced NF?B and AP-1 transcriptional activity associated with antiestrogen resistant breast cancer
    Article Snippet: .. The Renilla luciferase vector pRL-tk-luc (Promega, Madison, MI) was co-transfected to normalize for transfection efficiency. .. Culture media was changed 20 h following transfection and cells were then treated with PS341 (10 nM, 25 nM) or PA (1 μM, 5 μM), alone or in combination with 500 nM TAM for 24 h. Cells were subsequently washed with PBS, lysed for Dual-Glo™ luciferase assay (Promega), and reporter activity measured by luminometer.

    Article Title: Ascl2 activation by YAP1/KLF5 ensures the self-renewability of colon cancer progenitor cells
    Article Snippet: .. Transfection and luciferase assays The Ascl2 5′-flanking sequence (-2588/+620 bp region), amplified using PCR from intestinal tissue DNA, was inserted into the luciferase reporter vector pGL3-Basic (Promega, Madison, WI, USA). ..

    Article Title: Expression of a Homeostatic Regulator, Wip1 (Wild-type p53-induced Phosphatase), Is Temporally Induced by c-Jun and p53 in Response to UV Irradiation *
    Article Snippet: .. Cell were seeded on 12-well plates and transfected in Opti-MEM (11058-021; Invitrogen) for 4 h in the presence of different expression plasmids, 0.5 μg of a p53RE-containing reporter plasmid (p53-induced Luc; Stratagene) or Wip1–543-Luc, 0.01 μg of a Renilla luciferase vector (Promega) using the Lipofectamine 2000 (11668-019; Invitrogen), or NeonTM Transfection System (MPK5000; Invitrogen). .. Between 18- and 24-h post-transfection, cells were exposed to UV irradiation (50 J/m2 ).

    Luciferase:

    Article Title: Human Maf1 negatively regulates RNA Polymerase III transcription via the TFIIB family members Brf1 and Brf2
    Article Snippet: .. All luciferase results are reported as relative light units (RLU): the average of the Photinus pyralis firefly activity observed divided by the average of the activity recorded from Renilla luciferase vector. ..

    Article Title: ZNF521 sustains the differentiation block in MLL-rearranged acute myeloid leukemia
    Article Snippet: .. For luciferase assay, 293T cells were cotransfected with 0.5 μg of the reporter plasmid, 1 μg of expression plasmid or empty vector and 0.5 μg of Renilla luciferase reporter vector (Promega) as internal control for normalization of transfection efficiency, for a total of 2 μg of combined plasmids per well. .. The cells were then harvested at 48 hours after transfection using a Dual-Luciferase reporter assay system (Promega) and the Victor3 TM 1420 Multilabel Counter (PerkinElmer).

    Article Title: MiR-138 induces cell cycle arrest by targeting cyclin D3 in hepatocellular carcinoma
    Article Snippet: .. The PCR product was cloned into Firefly luciferase reporter vector pGL3 (Promega Corporation, Madison, WI), termed as pGL3-CCND3-3′UTR. .. The plasmid which carried the mutated sequence in the two complementary sites for the seed region of miR-138 ( ) was generated based on pGL3-CCND3-3′UTR plasmid by MutanBEST Kit (Takara Bio, Shiga, JP), termed as pGL3-CCND3-3′UTR-mut.

    Article Title: Ascl2 activation by YAP1/KLF5 ensures the self-renewability of colon cancer progenitor cells
    Article Snippet: .. Transfection and luciferase assays The Ascl2 5′-flanking sequence (-2588/+620 bp region), amplified using PCR from intestinal tissue DNA, was inserted into the luciferase reporter vector pGL3-Basic (Promega, Madison, WI, USA). ..

    Article Title: The E3 SUMO ligase PIASγ is a novel interaction partner regulating the activity of diabetes associated hepatocyte nuclear factor-1α
    Article Snippet: .. The Firefly luciferase reporter vector pGL3-RA (Promega), containing the promoter of the rat albumin gene (nucleotide −170 to + 5) and the pRL-SV40 reporter vector (Promega) encoding the Renilla luciferase gene were used in the transactivation assay . .. Luciferase assay MIN6 cells were transiently co-transfected with plasmids expressing WT or variant HNF1A cDNA and HA-SUMO3, Flag-PIASγ and reporter plasmids expressing Firefly and Renilla luciferase.

    Article Title: Expression of a Homeostatic Regulator, Wip1 (Wild-type p53-induced Phosphatase), Is Temporally Induced by c-Jun and p53 in Response to UV Irradiation *
    Article Snippet: .. Cell were seeded on 12-well plates and transfected in Opti-MEM (11058-021; Invitrogen) for 4 h in the presence of different expression plasmids, 0.5 μg of a p53RE-containing reporter plasmid (p53-induced Luc; Stratagene) or Wip1–543-Luc, 0.01 μg of a Renilla luciferase vector (Promega) using the Lipofectamine 2000 (11668-019; Invitrogen), or NeonTM Transfection System (MPK5000; Invitrogen). .. Between 18- and 24-h post-transfection, cells were exposed to UV irradiation (50 J/m2 ).

    Article Title: A Splice Variant of NCOR2, BQ323636.1, Confers Chemoresistance in Breast Cancer by Altering the Activity of NRF2
    Article Snippet: .. For promoter analysis, cells were collected 24 h post-transfection, washed twice in PBS, and harvested for Firefly/Renilla luciferase assays using the Dual-Luciferase reporter assay system (Promega, Madision, WI, USA) according to manufacturer’s instruction. .. Luminescence was then read using Infinite 200 Pro microplate plate reader (TECAN, Mannedorf, Switzerland).

    Reporter Assay:

    Article Title: A Splice Variant of NCOR2, BQ323636.1, Confers Chemoresistance in Breast Cancer by Altering the Activity of NRF2
    Article Snippet: .. For promoter analysis, cells were collected 24 h post-transfection, washed twice in PBS, and harvested for Firefly/Renilla luciferase assays using the Dual-Luciferase reporter assay system (Promega, Madision, WI, USA) according to manufacturer’s instruction. .. Luminescence was then read using Infinite 200 Pro microplate plate reader (TECAN, Mannedorf, Switzerland).

    Polymerase Chain Reaction:

    Article Title: MiR-138 induces cell cycle arrest by targeting cyclin D3 in hepatocellular carcinoma
    Article Snippet: .. The PCR product was cloned into Firefly luciferase reporter vector pGL3 (Promega Corporation, Madison, WI), termed as pGL3-CCND3-3′UTR. .. The plasmid which carried the mutated sequence in the two complementary sites for the seed region of miR-138 ( ) was generated based on pGL3-CCND3-3′UTR plasmid by MutanBEST Kit (Takara Bio, Shiga, JP), termed as pGL3-CCND3-3′UTR-mut.

    Article Title: Ascl2 activation by YAP1/KLF5 ensures the self-renewability of colon cancer progenitor cells
    Article Snippet: .. Transfection and luciferase assays The Ascl2 5′-flanking sequence (-2588/+620 bp region), amplified using PCR from intestinal tissue DNA, was inserted into the luciferase reporter vector pGL3-Basic (Promega, Madison, WI, USA). ..

    Transactivation Assay:

    Article Title: The E3 SUMO ligase PIASγ is a novel interaction partner regulating the activity of diabetes associated hepatocyte nuclear factor-1α
    Article Snippet: .. The Firefly luciferase reporter vector pGL3-RA (Promega), containing the promoter of the rat albumin gene (nucleotide −170 to + 5) and the pRL-SV40 reporter vector (Promega) encoding the Renilla luciferase gene were used in the transactivation assay . .. Luciferase assay MIN6 cells were transiently co-transfected with plasmids expressing WT or variant HNF1A cDNA and HA-SUMO3, Flag-PIASγ and reporter plasmids expressing Firefly and Renilla luciferase.

    Amplification:

    Article Title: Ascl2 activation by YAP1/KLF5 ensures the self-renewability of colon cancer progenitor cells
    Article Snippet: .. Transfection and luciferase assays The Ascl2 5′-flanking sequence (-2588/+620 bp region), amplified using PCR from intestinal tissue DNA, was inserted into the luciferase reporter vector pGL3-Basic (Promega, Madison, WI, USA). ..

    Activity Assay:

    Article Title: Human Maf1 negatively regulates RNA Polymerase III transcription via the TFIIB family members Brf1 and Brf2
    Article Snippet: .. All luciferase results are reported as relative light units (RLU): the average of the Photinus pyralis firefly activity observed divided by the average of the activity recorded from Renilla luciferase vector. ..

    Expressing:

    Article Title: ZNF521 sustains the differentiation block in MLL-rearranged acute myeloid leukemia
    Article Snippet: .. For luciferase assay, 293T cells were cotransfected with 0.5 μg of the reporter plasmid, 1 μg of expression plasmid or empty vector and 0.5 μg of Renilla luciferase reporter vector (Promega) as internal control for normalization of transfection efficiency, for a total of 2 μg of combined plasmids per well. .. The cells were then harvested at 48 hours after transfection using a Dual-Luciferase reporter assay system (Promega) and the Victor3 TM 1420 Multilabel Counter (PerkinElmer).

    Article Title: Expression of a Homeostatic Regulator, Wip1 (Wild-type p53-induced Phosphatase), Is Temporally Induced by c-Jun and p53 in Response to UV Irradiation *
    Article Snippet: .. Cell were seeded on 12-well plates and transfected in Opti-MEM (11058-021; Invitrogen) for 4 h in the presence of different expression plasmids, 0.5 μg of a p53RE-containing reporter plasmid (p53-induced Luc; Stratagene) or Wip1–543-Luc, 0.01 μg of a Renilla luciferase vector (Promega) using the Lipofectamine 2000 (11668-019; Invitrogen), or NeonTM Transfection System (MPK5000; Invitrogen). .. Between 18- and 24-h post-transfection, cells were exposed to UV irradiation (50 J/m2 ).

    Sequencing:

    Article Title: Ascl2 activation by YAP1/KLF5 ensures the self-renewability of colon cancer progenitor cells
    Article Snippet: .. Transfection and luciferase assays The Ascl2 5′-flanking sequence (-2588/+620 bp region), amplified using PCR from intestinal tissue DNA, was inserted into the luciferase reporter vector pGL3-Basic (Promega, Madison, WI, USA). ..

    Plasmid Preparation:

    Article Title: Human Maf1 negatively regulates RNA Polymerase III transcription via the TFIIB family members Brf1 and Brf2
    Article Snippet: .. All luciferase results are reported as relative light units (RLU): the average of the Photinus pyralis firefly activity observed divided by the average of the activity recorded from Renilla luciferase vector. ..

    Article Title: ZNF521 sustains the differentiation block in MLL-rearranged acute myeloid leukemia
    Article Snippet: .. For luciferase assay, 293T cells were cotransfected with 0.5 μg of the reporter plasmid, 1 μg of expression plasmid or empty vector and 0.5 μg of Renilla luciferase reporter vector (Promega) as internal control for normalization of transfection efficiency, for a total of 2 μg of combined plasmids per well. .. The cells were then harvested at 48 hours after transfection using a Dual-Luciferase reporter assay system (Promega) and the Victor3 TM 1420 Multilabel Counter (PerkinElmer).

    Article Title: MiR-138 induces cell cycle arrest by targeting cyclin D3 in hepatocellular carcinoma
    Article Snippet: .. The PCR product was cloned into Firefly luciferase reporter vector pGL3 (Promega Corporation, Madison, WI), termed as pGL3-CCND3-3′UTR. .. The plasmid which carried the mutated sequence in the two complementary sites for the seed region of miR-138 ( ) was generated based on pGL3-CCND3-3′UTR plasmid by MutanBEST Kit (Takara Bio, Shiga, JP), termed as pGL3-CCND3-3′UTR-mut.

    Article Title: Ascl2 activation by YAP1/KLF5 ensures the self-renewability of colon cancer progenitor cells
    Article Snippet: .. Transfection and luciferase assays The Ascl2 5′-flanking sequence (-2588/+620 bp region), amplified using PCR from intestinal tissue DNA, was inserted into the luciferase reporter vector pGL3-Basic (Promega, Madison, WI, USA). ..

    Article Title: The E3 SUMO ligase PIASγ is a novel interaction partner regulating the activity of diabetes associated hepatocyte nuclear factor-1α
    Article Snippet: .. The Firefly luciferase reporter vector pGL3-RA (Promega), containing the promoter of the rat albumin gene (nucleotide −170 to + 5) and the pRL-SV40 reporter vector (Promega) encoding the Renilla luciferase gene were used in the transactivation assay . .. Luciferase assay MIN6 cells were transiently co-transfected with plasmids expressing WT or variant HNF1A cDNA and HA-SUMO3, Flag-PIASγ and reporter plasmids expressing Firefly and Renilla luciferase.

    Article Title: Expression of a Homeostatic Regulator, Wip1 (Wild-type p53-induced Phosphatase), Is Temporally Induced by c-Jun and p53 in Response to UV Irradiation *
    Article Snippet: .. Cell were seeded on 12-well plates and transfected in Opti-MEM (11058-021; Invitrogen) for 4 h in the presence of different expression plasmids, 0.5 μg of a p53RE-containing reporter plasmid (p53-induced Luc; Stratagene) or Wip1–543-Luc, 0.01 μg of a Renilla luciferase vector (Promega) using the Lipofectamine 2000 (11668-019; Invitrogen), or NeonTM Transfection System (MPK5000; Invitrogen). .. Between 18- and 24-h post-transfection, cells were exposed to UV irradiation (50 J/m2 ).

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  • 91
    Promega luciferase activitie
    Luciferase Activitie, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luciferase activitie/product/Promega
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    luciferase activitie - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

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