ltq linear ion trap mass spectrometer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ltq linear ion trap mass spectrometer
    Ltq Linear Ion Trap Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 599 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ltq linear ion trap mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 599 article reviews
    Price from $9.99 to $1999.99
    ltq linear ion trap mass spectrometer - by Bioz Stars, 2020-02
    99/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Protective Effect of a Locked Retinal Chromophore Analog against Light-Induced Retinal Degeneration
    Article Snippet: Ten microliters of dissolved samples were injected into an Agilent Technology Santa Clara, CA) 1100 high-performance liquid chromatography system, and retinoids were separated on an xBridge C18 column (3.5 μ m, 2.1 × 100 mm; Waters, Milford, MA) by a linear gradient of acetonitrile in water (50%–100%, 30 minutes, at at flow rate of 0.5 ml/min). .. MS-based detection and quantification of 11- cis -6mr-retinal were performed with an LTQ linear ion trap mass spectrometer (Thermo Scientific) equipped with an electrospray ionization interface operated in the positive ionization mode.

    Article Title: The Inhibitory Role of α2,6-Sialylation in Adipogenesis *
    Article Snippet: .. Proteins were digested with modified trypsin, and the resulting peptides were subjected to LC/MS-MS. LC/MS-MS analysis was performed using an Advance Nano LC (Bruker-Michrom, Auburn, CA) and LTQ linear ion trap mass spectrometer (Thermo Fisher Scientific, Inc., San Jose, CA) equipped with a NANO HPLC capillary column C18 (0.075-mm inner diameter × 150-mm length, 3-mm particle size, Nikkyo Technos, Tokyo, Japan) using a linear gradient (25 min, 5–35% CH3 CN/0.1% formic acid) at a flow rate of 300 nl/min. .. The resulting MS and MS-MS data were searched against the Swiss-Prot database using MASCOT software (Matrix Science, London, UK).

    Article Title: RUNX proteins desensitize multiple myeloma to lenalidomide via protecting IKZFs from degradation
    Article Snippet: .. Loaded microcapillary columns were placed in-line with a Quaternary Agilent 1100 series HPLC pump and a LTQ linear ion trap mass spectrometer equipped with a nano-LC electrospray ionization source (Thermo Scientific). ..

    Flow Cytometry:

    Article Title: Protective Effect of a Locked Retinal Chromophore Analog against Light-Induced Retinal Degeneration
    Article Snippet: Ten microliters of dissolved samples were injected into an Agilent Technology Santa Clara, CA) 1100 high-performance liquid chromatography system, and retinoids were separated on an xBridge C18 column (3.5 μ m, 2.1 × 100 mm; Waters, Milford, MA) by a linear gradient of acetonitrile in water (50%–100%, 30 minutes, at at flow rate of 0.5 ml/min). .. MS-based detection and quantification of 11- cis -6mr-retinal were performed with an LTQ linear ion trap mass spectrometer (Thermo Scientific) equipped with an electrospray ionization interface operated in the positive ionization mode.

    Article Title: The Inhibitory Role of α2,6-Sialylation in Adipogenesis *
    Article Snippet: .. Proteins were digested with modified trypsin, and the resulting peptides were subjected to LC/MS-MS. LC/MS-MS analysis was performed using an Advance Nano LC (Bruker-Michrom, Auburn, CA) and LTQ linear ion trap mass spectrometer (Thermo Fisher Scientific, Inc., San Jose, CA) equipped with a NANO HPLC capillary column C18 (0.075-mm inner diameter × 150-mm length, 3-mm particle size, Nikkyo Technos, Tokyo, Japan) using a linear gradient (25 min, 5–35% CH3 CN/0.1% formic acid) at a flow rate of 300 nl/min. .. The resulting MS and MS-MS data were searched against the Swiss-Prot database using MASCOT software (Matrix Science, London, UK).

    Mass Spectrometry:

    Article Title: Protective Effect of a Locked Retinal Chromophore Analog against Light-Induced Retinal Degeneration
    Article Snippet: .. MS-based detection and quantification of 11- cis -6mr-retinal were performed with an LTQ linear ion trap mass spectrometer (Thermo Scientific) equipped with an electrospray ionization interface operated in the positive ionization mode. ..

    Article Title: The Inhibitory Role of α2,6-Sialylation in Adipogenesis *
    Article Snippet: .. Proteins were digested with modified trypsin, and the resulting peptides were subjected to LC/MS-MS. LC/MS-MS analysis was performed using an Advance Nano LC (Bruker-Michrom, Auburn, CA) and LTQ linear ion trap mass spectrometer (Thermo Fisher Scientific, Inc., San Jose, CA) equipped with a NANO HPLC capillary column C18 (0.075-mm inner diameter × 150-mm length, 3-mm particle size, Nikkyo Technos, Tokyo, Japan) using a linear gradient (25 min, 5–35% CH3 CN/0.1% formic acid) at a flow rate of 300 nl/min. .. The resulting MS and MS-MS data were searched against the Swiss-Prot database using MASCOT software (Matrix Science, London, UK).

    Article Title: RUNX proteins desensitize multiple myeloma to lenalidomide via protecting IKZFs from degradation
    Article Snippet: .. Loaded microcapillary columns were placed in-line with a Quaternary Agilent 1100 series HPLC pump and a LTQ linear ion trap mass spectrometer equipped with a nano-LC electrospray ionization source (Thermo Scientific). ..

    Article Title: Large-scale lipid analysis with C=C location and sn-position isomer resolving power
    Article Snippet: .. All MS4 experiments were performed on an LTQ linear ion trap mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA). ..

    Tandem Mass Spectroscopy:

    Article Title: The Inhibitory Role of α2,6-Sialylation in Adipogenesis *
    Article Snippet: Proteins were digested with modified trypsin, and the resulting peptides were subjected to LC/MS-MS. LC/MS-MS analysis was performed using an Advance Nano LC (Bruker-Michrom, Auburn, CA) and LTQ linear ion trap mass spectrometer (Thermo Fisher Scientific, Inc., San Jose, CA) equipped with a NANO HPLC capillary column C18 (0.075-mm inner diameter × 150-mm length, 3-mm particle size, Nikkyo Technos, Tokyo, Japan) using a linear gradient (25 min, 5–35% CH3 CN/0.1% formic acid) at a flow rate of 300 nl/min. .. The resulting MS and MS-MS data were searched against the Swiss-Prot database using MASCOT software (Matrix Science, London, UK).

    Article Title: RUNX proteins desensitize multiple myeloma to lenalidomide via protecting IKZFs from degradation
    Article Snippet: Loaded microcapillary columns were placed in-line with a Quaternary Agilent 1100 series HPLC pump and a LTQ linear ion trap mass spectrometer equipped with a nano-LC electrospray ionization source (Thermo Scientific). .. Tandem mass (MS/MS) spectra were interpreted using SEQUEST [ ] against a database of 72,956 sequences, consisting of 72,968 non-redundant human proteins (downloaded from NCBI on 2015-03-25), 148 usual contaminants (such as human keratins, IgGs, and proteolytic enzymes), and to estimate false-discovery rates, 73,091 randomized amino-acid sequences derived from each non-redundant protein entry.

    Modification:

    Article Title: The Inhibitory Role of α2,6-Sialylation in Adipogenesis *
    Article Snippet: .. Proteins were digested with modified trypsin, and the resulting peptides were subjected to LC/MS-MS. LC/MS-MS analysis was performed using an Advance Nano LC (Bruker-Michrom, Auburn, CA) and LTQ linear ion trap mass spectrometer (Thermo Fisher Scientific, Inc., San Jose, CA) equipped with a NANO HPLC capillary column C18 (0.075-mm inner diameter × 150-mm length, 3-mm particle size, Nikkyo Technos, Tokyo, Japan) using a linear gradient (25 min, 5–35% CH3 CN/0.1% formic acid) at a flow rate of 300 nl/min. .. The resulting MS and MS-MS data were searched against the Swiss-Prot database using MASCOT software (Matrix Science, London, UK).

    Article Title: RUNX proteins desensitize multiple myeloma to lenalidomide via protecting IKZFs from degradation
    Article Snippet: MudPIT analysis TCA-precipitates were urea-denatured, reduced, alkylated, and digested with endoproteinase Lys-C (Roche), followed by modified trypsin (Roche) [ , ]. .. Loaded microcapillary columns were placed in-line with a Quaternary Agilent 1100 series HPLC pump and a LTQ linear ion trap mass spectrometer equipped with a nano-LC electrospray ionization source (Thermo Scientific).

    Injection:

    Article Title: Protective Effect of a Locked Retinal Chromophore Analog against Light-Induced Retinal Degeneration
    Article Snippet: Ten microliters of dissolved samples were injected into an Agilent Technology Santa Clara, CA) 1100 high-performance liquid chromatography system, and retinoids were separated on an xBridge C18 column (3.5 μ m, 2.1 × 100 mm; Waters, Milford, MA) by a linear gradient of acetonitrile in water (50%–100%, 30 minutes, at at flow rate of 0.5 ml/min). .. MS-based detection and quantification of 11- cis -6mr-retinal were performed with an LTQ linear ion trap mass spectrometer (Thermo Scientific) equipped with an electrospray ionization interface operated in the positive ionization mode.

    Derivative Assay:

    Article Title: RUNX proteins desensitize multiple myeloma to lenalidomide via protecting IKZFs from degradation
    Article Snippet: Loaded microcapillary columns were placed in-line with a Quaternary Agilent 1100 series HPLC pump and a LTQ linear ion trap mass spectrometer equipped with a nano-LC electrospray ionization source (Thermo Scientific). .. Tandem mass (MS/MS) spectra were interpreted using SEQUEST [ ] against a database of 72,956 sequences, consisting of 72,968 non-redundant human proteins (downloaded from NCBI on 2015-03-25), 148 usual contaminants (such as human keratins, IgGs, and proteolytic enzymes), and to estimate false-discovery rates, 73,091 randomized amino-acid sequences derived from each non-redundant protein entry.

    Software:

    Article Title: The Inhibitory Role of α2,6-Sialylation in Adipogenesis *
    Article Snippet: Proteins were digested with modified trypsin, and the resulting peptides were subjected to LC/MS-MS. LC/MS-MS analysis was performed using an Advance Nano LC (Bruker-Michrom, Auburn, CA) and LTQ linear ion trap mass spectrometer (Thermo Fisher Scientific, Inc., San Jose, CA) equipped with a NANO HPLC capillary column C18 (0.075-mm inner diameter × 150-mm length, 3-mm particle size, Nikkyo Technos, Tokyo, Japan) using a linear gradient (25 min, 5–35% CH3 CN/0.1% formic acid) at a flow rate of 300 nl/min. .. The resulting MS and MS-MS data were searched against the Swiss-Prot database using MASCOT software (Matrix Science, London, UK).

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    Thermo Fisher hybrid linear ion trap orbitrap mass spectrometer
    Using internal standard-assisted synthesis and degradation mass spectrometry (iSDMS) to study the roles of EGF on global protein synthesis and degradation. SKBR3_shVPS4B and the parental SKBR3 cells were first labeled with 13 C 6 -arginine (Arg6) and D 4 -lysine (Lys4) medium (labeled in red). After overnight serum starvation, Arg6/Lys4-labeled cells were stimulated with 100 ng/mL EGF in medium supplemented with regular arginine (Arg0) and lysine (Lys0) for 0, 2, 6, 12, and 24 hr (labeled in blue). Protein isolated from SKBR3_shVPS4B cells labeled with 13 C 6 15 N 4 -arginine (Arg10) and 13 C 6 15 N 2 -lysine (Lys8)—internal standard (labeled in green)—was spiked into each sample at a ratio of 1 : 3 (wt/wt). The mixtures were digested by the Filter Aided Sample Preparation (FASP) procedure, followed by strong anion exchange (SAX) peptide fractionation. Peptides were analyzed by online LC-MS/MS using an <t>LTQ-Orbitrap</t> mass spectrometer. The relative abundance of the newly synthesized ( A l ) or preexisting peptides ( A m ) was defined as the ratio of mass spectrometric peak intensities of the unlabeled peptides ( I l ) or Arg6/Lys4-labeled peptides ( I m ) to the intensities of the Arg10/Lys8-labeled peptides ( I h ), respectively.
    Hybrid Linear Ion Trap Orbitrap Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 178 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hybrid linear ion trap orbitrap mass spectrometer/product/Thermo Fisher
    Average 90 stars, based on 178 article reviews
    Price from $9.99 to $1999.99
    hybrid linear ion trap orbitrap mass spectrometer - by Bioz Stars, 2020-02
    90/100 stars
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    76
    Thermo Fisher hybrid ltq linear ion trap
    Peptide identifications from liver and brain tissue. The number (y-axis) of phosphorylated and unmodified proteins identified from brain and liver on a <t>LTQ-Orbitrap</t> mass spectrometer following the phosphopeptide enrichment using IMAC.
    Hybrid Ltq Linear Ion Trap, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 76/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hybrid ltq linear ion trap/product/Thermo Fisher
    Average 76 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    99
    Thermo Fisher hybrid linear ion trap ft icr mass spectrometer
    Mass spectrometry characterization of rhamnosylated EF-P. (A) A mass spectrum of His6–EF-P protein, recorded on a <t>7T</t> <t>FT-ICR</t> instrument, from which protein molecular masses were calculated. (B) Lys-C-digested peptide fragmented by ETD maps the additional mass of 146.057 Da on Arg32. The precursor ion, m / z 349.865, is indicated by a dashed line. (C) A proposed fragmentation pattern based on ETD-HCD MS 3 data from the c3 + ion. The neutral losses are colored uniquely to associate the fragment ion with the hypothetical structure. The asterisk indicates a background ion. The precursor ion, m / z 464.246, is indicated by a dashed line.
    Hybrid Linear Ion Trap Ft Icr Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hybrid linear ion trap ft icr mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    hybrid linear ion trap ft icr mass spectrometer - by Bioz Stars, 2020-02
    99/100 stars
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    Using internal standard-assisted synthesis and degradation mass spectrometry (iSDMS) to study the roles of EGF on global protein synthesis and degradation. SKBR3_shVPS4B and the parental SKBR3 cells were first labeled with 13 C 6 -arginine (Arg6) and D 4 -lysine (Lys4) medium (labeled in red). After overnight serum starvation, Arg6/Lys4-labeled cells were stimulated with 100 ng/mL EGF in medium supplemented with regular arginine (Arg0) and lysine (Lys0) for 0, 2, 6, 12, and 24 hr (labeled in blue). Protein isolated from SKBR3_shVPS4B cells labeled with 13 C 6 15 N 4 -arginine (Arg10) and 13 C 6 15 N 2 -lysine (Lys8)—internal standard (labeled in green)—was spiked into each sample at a ratio of 1 : 3 (wt/wt). The mixtures were digested by the Filter Aided Sample Preparation (FASP) procedure, followed by strong anion exchange (SAX) peptide fractionation. Peptides were analyzed by online LC-MS/MS using an LTQ-Orbitrap mass spectrometer. The relative abundance of the newly synthesized ( A l ) or preexisting peptides ( A m ) was defined as the ratio of mass spectrometric peak intensities of the unlabeled peptides ( I l ) or Arg6/Lys4-labeled peptides ( I m ) to the intensities of the Arg10/Lys8-labeled peptides ( I h ), respectively.

    Journal: International Journal of Proteomics

    Article Title: An Internal Standard-Assisted Synthesis and Degradation Proteomic Approach Reveals the Potential Linkage between VPS4B Depletion and Activation of Fatty Acid β-Oxidation in Breast Cancer Cells

    doi: 10.1155/2013/291415

    Figure Lengend Snippet: Using internal standard-assisted synthesis and degradation mass spectrometry (iSDMS) to study the roles of EGF on global protein synthesis and degradation. SKBR3_shVPS4B and the parental SKBR3 cells were first labeled with 13 C 6 -arginine (Arg6) and D 4 -lysine (Lys4) medium (labeled in red). After overnight serum starvation, Arg6/Lys4-labeled cells were stimulated with 100 ng/mL EGF in medium supplemented with regular arginine (Arg0) and lysine (Lys0) for 0, 2, 6, 12, and 24 hr (labeled in blue). Protein isolated from SKBR3_shVPS4B cells labeled with 13 C 6 15 N 4 -arginine (Arg10) and 13 C 6 15 N 2 -lysine (Lys8)—internal standard (labeled in green)—was spiked into each sample at a ratio of 1 : 3 (wt/wt). The mixtures were digested by the Filter Aided Sample Preparation (FASP) procedure, followed by strong anion exchange (SAX) peptide fractionation. Peptides were analyzed by online LC-MS/MS using an LTQ-Orbitrap mass spectrometer. The relative abundance of the newly synthesized ( A l ) or preexisting peptides ( A m ) was defined as the ratio of mass spectrometric peak intensities of the unlabeled peptides ( I l ) or Arg6/Lys4-labeled peptides ( I m ) to the intensities of the Arg10/Lys8-labeled peptides ( I h ), respectively.

    Article Snippet: LC-MS/MS Analysis Peptides were dissolved in 0.5% acetic acid (solvent A) and separated on a 10 cm reverse-phase PicoFrit spray tip (New Objective, Woburn, MA) packed in house with sub-2 μ m C18 resin (Prospereon Life Science, IL), using a nanoflow Xtreme simple liquid chromatography system (Microtech/CVC) coupled to a hybrid linear ion trap-Orbitrap mass spectrometer (LTQ Orbitrap, Thermo Scientific).

    Techniques: Mass Spectrometry, Labeling, Isolation, Sample Prep, Peptide Fractionation, Liquid Chromatography with Mass Spectroscopy, Synthesized

    Influence of the potential applied to the ESI source on retention of UDP-sugars. Chromatogram obtained with a newly installed or regenerated PGC column ( a ); chromatogram obtained after 20-min utilization of the column connected to the ESI source of an Orbitrap mass spectrometer ( b ). Conditions: column regeneration was performed with 80 % acetonitrile in water containing 0.10 % trifluoroacetic acid for 20 min, the sample measurements were done with the gradient program described in the materials and methods section for HPLC-MS; detection, UV, 262 nm; sample. UDP-Gal (100 μmol L −1 ) and UDP-Glc (100 μmol L −1 )

    Journal: Analytical and Bioanalytical Chemistry

    Article Title: Quantitative HPLC-MS analysis of nucleotide sugars in plant cells following off-line SPE sample preparation

    doi: 10.1007/s00216-014-7746-3

    Figure Lengend Snippet: Influence of the potential applied to the ESI source on retention of UDP-sugars. Chromatogram obtained with a newly installed or regenerated PGC column ( a ); chromatogram obtained after 20-min utilization of the column connected to the ESI source of an Orbitrap mass spectrometer ( b ). Conditions: column regeneration was performed with 80 % acetonitrile in water containing 0.10 % trifluoroacetic acid for 20 min, the sample measurements were done with the gradient program described in the materials and methods section for HPLC-MS; detection, UV, 262 nm; sample. UDP-Gal (100 μmol L −1 ) and UDP-Glc (100 μmol L −1 )

    Article Snippet: Fragmentation experiments were performed on a linear ion trap–Orbitrap mass spectrometer (Model LTQ Orbitrap XL, Thermo Fisher Scientific).

    Techniques: Pyrolysis Gas Chromatography, Mass Spectrometry, High Performance Liquid Chromatography, Gas Chromatography

    Peptide identifications from liver and brain tissue. The number (y-axis) of phosphorylated and unmodified proteins identified from brain and liver on a LTQ-Orbitrap mass spectrometer following the phosphopeptide enrichment using IMAC.

    Journal: PLoS ONE

    Article Title: Differential Proteomic Analysis of Mammalian Tissues Using SILAM

    doi: 10.1371/journal.pone.0016039

    Figure Lengend Snippet: Peptide identifications from liver and brain tissue. The number (y-axis) of phosphorylated and unmodified proteins identified from brain and liver on a LTQ-Orbitrap mass spectrometer following the phosphopeptide enrichment using IMAC.

    Article Snippet: As peptides were eluted from the microcapillary column they were electrosprayed directly into a hybrid LTQ linear ion trap and Orbitrap (ThermoFisher, San Jose, CA) with the application of a distal 2.4 kV spray voltage.

    Techniques: Mass Spectrometry

    Mass spectrometry characterization of rhamnosylated EF-P. (A) A mass spectrum of His6–EF-P protein, recorded on a 7T FT-ICR instrument, from which protein molecular masses were calculated. (B) Lys-C-digested peptide fragmented by ETD maps the additional mass of 146.057 Da on Arg32. The precursor ion, m / z 349.865, is indicated by a dashed line. (C) A proposed fragmentation pattern based on ETD-HCD MS 3 data from the c3 + ion. The neutral losses are colored uniquely to associate the fragment ion with the hypothetical structure. The asterisk indicates a background ion. The precursor ion, m / z 464.246, is indicated by a dashed line.

    Journal: mBio

    Article Title: Cyclic Rhamnosylated Elongation Factor P Establishes Antibiotic Resistance in Pseudomonas aeruginosa

    doi: 10.1128/mBio.00823-15

    Figure Lengend Snippet: Mass spectrometry characterization of rhamnosylated EF-P. (A) A mass spectrum of His6–EF-P protein, recorded on a 7T FT-ICR instrument, from which protein molecular masses were calculated. (B) Lys-C-digested peptide fragmented by ETD maps the additional mass of 146.057 Da on Arg32. The precursor ion, m / z 349.865, is indicated by a dashed line. (C) A proposed fragmentation pattern based on ETD-HCD MS 3 data from the c3 + ion. The neutral losses are colored uniquely to associate the fragment ion with the hypothetical structure. The asterisk indicates a background ion. The precursor ion, m / z 464.246, is indicated by a dashed line.

    Article Snippet: All samples were analyzed using a hybrid linear ion-trap/FT-ICR mass spectrometer (7T, LTQ FT Ultra; Thermo Scientific) operated with a standard (up to m /z 2,000) or extended (up to m /z 4,000) mass range.

    Techniques: Mass Spectrometry