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Carl Zeiss gfp expression
<t>cyp-36A1</t> expression in multiple tissues rescues the egg-laying phenotype of cyp-36A1(lf); egl-9(lf) . ( A–H ) Distribution of stages of eggs laid by adult hermaphrodites of the indicated genotypes. All strains contained the agIs219 ( P T24B8.5 <t>::gfp</t> ) transgene. ( A ) Stages of eggs laid by wild-type animals. ( B ) egl-9(sa307) animals laid later stage eggs than wild type (p
Gfp Expression, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 92/100, based on 2732 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Hypoxia-inducible factor cell non-autonomously regulates C. elegans stress responses and behavior via a nuclear receptor"

Article Title: Hypoxia-inducible factor cell non-autonomously regulates C. elegans stress responses and behavior via a nuclear receptor

Journal: eLife

doi: 10.7554/eLife.36828

cyp-36A1 expression in multiple tissues rescues the egg-laying phenotype of cyp-36A1(lf); egl-9(lf) . ( A–H ) Distribution of stages of eggs laid by adult hermaphrodites of the indicated genotypes. All strains contained the agIs219 ( P T24B8.5 ::gfp ) transgene. ( A ) Stages of eggs laid by wild-type animals. ( B ) egl-9(sa307) animals laid later stage eggs than wild type (p
Figure Legend Snippet: cyp-36A1 expression in multiple tissues rescues the egg-laying phenotype of cyp-36A1(lf); egl-9(lf) . ( A–H ) Distribution of stages of eggs laid by adult hermaphrodites of the indicated genotypes. All strains contained the agIs219 ( P T24B8.5 ::gfp ) transgene. ( A ) Stages of eggs laid by wild-type animals. ( B ) egl-9(sa307) animals laid later stage eggs than wild type (p

Techniques Used: Expressing

cyp-36A1 is expressed in many tissues. ( A–D ) Paired fluorescent (left in each panel) and Nomarski (right in each panel) micrographs showing expression of a transcriptional P cyp-36A1 ::gfp reporter ( nIs682 ) in an adult worm. Expression was observed in the head ( A ), midbody ( B and C ), and tail ( D ), including in neurons, body-wall muscle, vulval muscle, intestine, and hypoderm, as indicated. Scale bars, 20 μm.
Figure Legend Snippet: cyp-36A1 is expressed in many tissues. ( A–D ) Paired fluorescent (left in each panel) and Nomarski (right in each panel) micrographs showing expression of a transcriptional P cyp-36A1 ::gfp reporter ( nIs682 ) in an adult worm. Expression was observed in the head ( A ), midbody ( B and C ), and tail ( D ), including in neurons, body-wall muscle, vulval muscle, intestine, and hypoderm, as indicated. Scale bars, 20 μm.

Techniques Used: Expressing

2) Product Images from "Neurohormonal signaling via a sulfotransferase antagonizes insulin-like signaling to regulate a Caenorhabditis elegans stress response"

Article Title: Neurohormonal signaling via a sulfotransferase antagonizes insulin-like signaling to regulate a Caenorhabditis elegans stress response

Journal: Nature Communications

doi: 10.1038/s41467-018-07640-w

SSU-1 and insulin-like signaling via the FOXO transcription factor DAF-16 function in parallel to regulate gene expression and developmental arrest in response to osmotic stress. a Average fold change mRNA expression in the wild-type and daf-16(m26) mutant embryos at 500 mM NaCl as compared to 50 mM NaCl measured by RNA-seq (FPKM 500 mM NaCl/FPKM 50 mM NaCl + 1). Shown are the 25 genes that exhibited the greatest increase in expression in wild-type animals at 500 mM NaCl vs. 50 mM NaCl. n = 3 replicates. Error bars, s.d. b Comparison of the 20 genes exhibiting the largest SSU-1 and DAF-16-dependent increases in expression in response to 500 mM NaCl. c Venn diagram of genes with a greater than twofold increase in RNA expression in response to osmotic stress and dependent on SSU-1 (blue) and/or DAF-16 (yellow). p -value represents normal approximation to the hypergeometric probability (See Statistics and Reproducibility). d Percent of wild-type, daf-2(e1370) , ssu-1(fc73) , and nhr-1(n6242) mutant embryos failing to arrest development in response to 300 mM NaCl. n > 100 animals. Error bars, s.d. e Percent of wild-type, daf-2(e1370) , ssu-1(fc73) , and nhr-1(n6242) mutant embryos failing to arrest development in response to 500 mM NaCl. n > 100 animals. Error bars, s.d. f Representative confocal images of DAF-16::GFP localization after 5 h of exposure to osmotic stress (500 mM NaCl) in the wild-type and ssu-1(fc73) mutant embryos. Scale bars, 10 µm. Source data are provided as a Source Data file
Figure Legend Snippet: SSU-1 and insulin-like signaling via the FOXO transcription factor DAF-16 function in parallel to regulate gene expression and developmental arrest in response to osmotic stress. a Average fold change mRNA expression in the wild-type and daf-16(m26) mutant embryos at 500 mM NaCl as compared to 50 mM NaCl measured by RNA-seq (FPKM 500 mM NaCl/FPKM 50 mM NaCl + 1). Shown are the 25 genes that exhibited the greatest increase in expression in wild-type animals at 500 mM NaCl vs. 50 mM NaCl. n = 3 replicates. Error bars, s.d. b Comparison of the 20 genes exhibiting the largest SSU-1 and DAF-16-dependent increases in expression in response to 500 mM NaCl. c Venn diagram of genes with a greater than twofold increase in RNA expression in response to osmotic stress and dependent on SSU-1 (blue) and/or DAF-16 (yellow). p -value represents normal approximation to the hypergeometric probability (See Statistics and Reproducibility). d Percent of wild-type, daf-2(e1370) , ssu-1(fc73) , and nhr-1(n6242) mutant embryos failing to arrest development in response to 300 mM NaCl. n > 100 animals. Error bars, s.d. e Percent of wild-type, daf-2(e1370) , ssu-1(fc73) , and nhr-1(n6242) mutant embryos failing to arrest development in response to 500 mM NaCl. n > 100 animals. Error bars, s.d. f Representative confocal images of DAF-16::GFP localization after 5 h of exposure to osmotic stress (500 mM NaCl) in the wild-type and ssu-1(fc73) mutant embryos. Scale bars, 10 µm. Source data are provided as a Source Data file

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay, RNA Expression

3) Product Images from "Gellan Gum Promotes the Differentiation of Enterocytes from Human Induced Pluripotent Stem Cells"

Article Title: Gellan Gum Promotes the Differentiation of Enterocytes from Human Induced Pluripotent Stem Cells

Journal: Pharmaceutics

doi: 10.3390/pharmaceutics12100951

Mechanistic analysis of the effect of GG in enterocyte differentiation. ( a ) Immunofluorescence images of the senescence marker β-galactosidase. ( b ) Relative percentage of β-galactosidase-positive cells. ( c ) Relative mRNA expression levels of p21 Cip1 . ( d ) Relative mRNA expression levels of integrin α5. ( e ) Immunofluorescence images of integrin α5. ( f ) Western blotting analysis of integrin α5 and relative protein expression. ( g ) Proposed scheme showing the relationship between p21 Cip1 and senescence. Scale bars = 100 μm. All data are presented as mean ± S.D. ( n = 3). Control = 1. Levels of statistical significance compared with the control group: * p
Figure Legend Snippet: Mechanistic analysis of the effect of GG in enterocyte differentiation. ( a ) Immunofluorescence images of the senescence marker β-galactosidase. ( b ) Relative percentage of β-galactosidase-positive cells. ( c ) Relative mRNA expression levels of p21 Cip1 . ( d ) Relative mRNA expression levels of integrin α5. ( e ) Immunofluorescence images of integrin α5. ( f ) Western blotting analysis of integrin α5 and relative protein expression. ( g ) Proposed scheme showing the relationship between p21 Cip1 and senescence. Scale bars = 100 μm. All data are presented as mean ± S.D. ( n = 3). Control = 1. Levels of statistical significance compared with the control group: * p

Techniques Used: Immunofluorescence, Marker, Expressing, Western Blot

4) Product Images from "Neurohormonal signaling via a sulfotransferase antagonizes insulin-like signaling to regulate a Caenorhabditis elegans stress response"

Article Title: Neurohormonal signaling via a sulfotransferase antagonizes insulin-like signaling to regulate a Caenorhabditis elegans stress response

Journal: Nature Communications

doi: 10.1038/s41467-018-07640-w

The nuclear hormone receptor NHR-1 is required for SSU-1 to promote the transcriptional response to osmotic stress. a Average fold change in mRNA expression in wild-type and ssu-1(fc73) mutant embryos at 500 mM NaCl as compared to 50 mM NaCl measured by RNA-seq (FPKM 500 mM NaCl/FPKM 50 mM NaCl + 1). Shown are the 25 genes that exhibited the greatest increase in expression in wild-type animals at 500 mM NaCl vs. 50 mM NaCl. n = 3 replicates. Error bars, s.d. b Confocal and differential interference contrast (DIC) images showing Psod-5::gfp expression in wild-type and ssu-1(fc73) L1-stage mutants exposed to 500 mM NaCl for 24 h. Scale bars, 100 µm. c Confocal and DIC images of Psod-5::GFP expression in wild-type, ssu-1(fc73) , and ssu-1(fc73); ssu-1( + ) L1-stage mutants exposed to 500 mM NaCl for 24 h. The trx-1 promoter was used to drive ASJ cell-specific expression of SSU-1. Scale bars, 100 µm. d Schematic of nhr-1 mutations that cause defects in developmental arrest in response to osmotic stress. Scale bar, 100 bp. fs: frameshift. Numbers represent amino acid numbers. e Percent of nhr-1 mutants failing to arrest development in response to 500 mM NaCl. n = 3 experiments, each with more than 100 animals. Error bars, s.d. f Representative confocal and DIC images of Psod-5::GFP expression in wild-type and nhr-1(n6242) mutant dauers. The ges-1 promoter was used to drive intestine-specific expression, the rab-3 promoter was used to drive neuron-specific expression, and the unc-54 promoter was used to drive muscle-specific expression. Scale bar, 100 µm. g Representative confocal and DIC images of Psod-5::GFP expression in wild-type and nhr-1(n6242) L1-stage animals. The trx-1 promoter was used to drive ASJ cell-specific expression of SSU-1. Animals expressed GFP specifically in coelomocytes under the control of the unc-122 promoter as a co-injection marker. %, percent of animals that expressed GFP as in the representative image shown. Scale bar, 100 µm. * p
Figure Legend Snippet: The nuclear hormone receptor NHR-1 is required for SSU-1 to promote the transcriptional response to osmotic stress. a Average fold change in mRNA expression in wild-type and ssu-1(fc73) mutant embryos at 500 mM NaCl as compared to 50 mM NaCl measured by RNA-seq (FPKM 500 mM NaCl/FPKM 50 mM NaCl + 1). Shown are the 25 genes that exhibited the greatest increase in expression in wild-type animals at 500 mM NaCl vs. 50 mM NaCl. n = 3 replicates. Error bars, s.d. b Confocal and differential interference contrast (DIC) images showing Psod-5::gfp expression in wild-type and ssu-1(fc73) L1-stage mutants exposed to 500 mM NaCl for 24 h. Scale bars, 100 µm. c Confocal and DIC images of Psod-5::GFP expression in wild-type, ssu-1(fc73) , and ssu-1(fc73); ssu-1( + ) L1-stage mutants exposed to 500 mM NaCl for 24 h. The trx-1 promoter was used to drive ASJ cell-specific expression of SSU-1. Scale bars, 100 µm. d Schematic of nhr-1 mutations that cause defects in developmental arrest in response to osmotic stress. Scale bar, 100 bp. fs: frameshift. Numbers represent amino acid numbers. e Percent of nhr-1 mutants failing to arrest development in response to 500 mM NaCl. n = 3 experiments, each with more than 100 animals. Error bars, s.d. f Representative confocal and DIC images of Psod-5::GFP expression in wild-type and nhr-1(n6242) mutant dauers. The ges-1 promoter was used to drive intestine-specific expression, the rab-3 promoter was used to drive neuron-specific expression, and the unc-54 promoter was used to drive muscle-specific expression. Scale bar, 100 µm. g Representative confocal and DIC images of Psod-5::GFP expression in wild-type and nhr-1(n6242) L1-stage animals. The trx-1 promoter was used to drive ASJ cell-specific expression of SSU-1. Animals expressed GFP specifically in coelomocytes under the control of the unc-122 promoter as a co-injection marker. %, percent of animals that expressed GFP as in the representative image shown. Scale bar, 100 µm. * p

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay, Injection, Marker

SSU-1 and insulin-like signaling via the FOXO transcription factor DAF-16 function in parallel to regulate gene expression and developmental arrest in response to osmotic stress. a Average fold change mRNA expression in the wild-type and daf-16(m26) mutant embryos at 500 mM NaCl as compared to 50 mM NaCl measured by RNA-seq (FPKM 500 mM NaCl/FPKM 50 mM NaCl + 1). Shown are the 25 genes that exhibited the greatest increase in expression in wild-type animals at 500 mM NaCl vs. 50 mM NaCl. n = 3 replicates. Error bars, s.d. b Comparison of the 20 genes exhibiting the largest SSU-1 and DAF-16-dependent increases in expression in response to 500 mM NaCl. c Venn diagram of genes with a greater than twofold increase in RNA expression in response to osmotic stress and dependent on SSU-1 (blue) and/or DAF-16 (yellow). p -value represents normal approximation to the hypergeometric probability (See Statistics and Reproducibility). d Percent of wild-type, daf-2(e1370) , ssu-1(fc73) , and nhr-1(n6242) mutant embryos failing to arrest development in response to 300 mM NaCl. n > 100 animals. Error bars, s.d. e Percent of wild-type, daf-2(e1370) , ssu-1(fc73) , and nhr-1(n6242) mutant embryos failing to arrest development in response to 500 mM NaCl. n > 100 animals. Error bars, s.d. f Representative confocal images of DAF-16::GFP localization after 5 h of exposure to osmotic stress (500 mM NaCl) in the wild-type and ssu-1(fc73) mutant embryos. Scale bars, 10 µm. Source data are provided as a Source Data file
Figure Legend Snippet: SSU-1 and insulin-like signaling via the FOXO transcription factor DAF-16 function in parallel to regulate gene expression and developmental arrest in response to osmotic stress. a Average fold change mRNA expression in the wild-type and daf-16(m26) mutant embryos at 500 mM NaCl as compared to 50 mM NaCl measured by RNA-seq (FPKM 500 mM NaCl/FPKM 50 mM NaCl + 1). Shown are the 25 genes that exhibited the greatest increase in expression in wild-type animals at 500 mM NaCl vs. 50 mM NaCl. n = 3 replicates. Error bars, s.d. b Comparison of the 20 genes exhibiting the largest SSU-1 and DAF-16-dependent increases in expression in response to 500 mM NaCl. c Venn diagram of genes with a greater than twofold increase in RNA expression in response to osmotic stress and dependent on SSU-1 (blue) and/or DAF-16 (yellow). p -value represents normal approximation to the hypergeometric probability (See Statistics and Reproducibility). d Percent of wild-type, daf-2(e1370) , ssu-1(fc73) , and nhr-1(n6242) mutant embryos failing to arrest development in response to 300 mM NaCl. n > 100 animals. Error bars, s.d. e Percent of wild-type, daf-2(e1370) , ssu-1(fc73) , and nhr-1(n6242) mutant embryos failing to arrest development in response to 500 mM NaCl. n > 100 animals. Error bars, s.d. f Representative confocal images of DAF-16::GFP localization after 5 h of exposure to osmotic stress (500 mM NaCl) in the wild-type and ssu-1(fc73) mutant embryos. Scale bars, 10 µm. Source data are provided as a Source Data file

Techniques Used: Expressing, Mutagenesis, RNA Sequencing Assay, RNA Expression

The cytosolic sulfotransferase SSU-1 functions in the ASJ sensory neurons to regulate developmental arrest in response to osmotic stress. a Schematic of ssu-1 mutations that cause defects in developmental arrest in response to osmotic stress. Scale bar, 100 bp. Numbers represent amino acid numbers. b Percent of ssu-1 mutants failing to arrest development in response to 500 mM NaCl. n = 3 experiments, each with more than 100 animals. Error bars, s.d. c Percent of wild-type, daf-16(mu86) , and ssu-1(fc73 ) animals with a divided M cell after 1 week of starvation. Animals contained ayIs7 [hlh-8::GFP fusion + dpy-20( + )] to facilitate M-cell identification. n > 200 animals. Error bars, s.e.m. d Number of wild-type, ssu-1(fc73) , and ssu-1(fc73); ssu-1( + ) animals failing to arrest development in response to osmotic stress. The trx-1 promoter was used to drive ASJ cell-specific expression of ssu-1 . Animals also contained wuIs57 [pPD95.77 Psod-5::GFP, rol-6(su1006)] , which drives GFP expression in response to osmotic stress. n = 100 animals. * p
Figure Legend Snippet: The cytosolic sulfotransferase SSU-1 functions in the ASJ sensory neurons to regulate developmental arrest in response to osmotic stress. a Schematic of ssu-1 mutations that cause defects in developmental arrest in response to osmotic stress. Scale bar, 100 bp. Numbers represent amino acid numbers. b Percent of ssu-1 mutants failing to arrest development in response to 500 mM NaCl. n = 3 experiments, each with more than 100 animals. Error bars, s.d. c Percent of wild-type, daf-16(mu86) , and ssu-1(fc73 ) animals with a divided M cell after 1 week of starvation. Animals contained ayIs7 [hlh-8::GFP fusion + dpy-20( + )] to facilitate M-cell identification. n > 200 animals. Error bars, s.e.m. d Number of wild-type, ssu-1(fc73) , and ssu-1(fc73); ssu-1( + ) animals failing to arrest development in response to osmotic stress. The trx-1 promoter was used to drive ASJ cell-specific expression of ssu-1 . Animals also contained wuIs57 [pPD95.77 Psod-5::GFP, rol-6(su1006)] , which drives GFP expression in response to osmotic stress. n = 100 animals. * p

Techniques Used: Expressing

5) Product Images from "Insulin-like signalling to the maternal germline controls progeny response to osmotic stress"

Article Title: Insulin-like signalling to the maternal germline controls progeny response to osmotic stress

Journal: Nature cell biology

doi: 10.1038/ncb3470

Insulin-like signalling to the maternal germline regulates progeny response to osmotic stress (a) Percent of wild-type, daf-2(e1370) and daf-2(e1370); daf-16(mu86) cross progeny failing to arrest development after 48 hrs at 500 mM NaCl. Males contained (Pegl-1:: 4xNLS::GFP ); him-5(e1490); nIs349 (Pceh-28:: 4xNLS::mCherry) for the identification of cross progeny. The pie-1 promoter was used to drive germline specific expression of DAF-2 and the mex-5 promoter was used to drive germline specific expression of DAF-16. Error bars, s.d. n = 7, 3, 6, 3, 3, 3, and 3 see Supplementary Table 6 . (b) Percent of wild-type, gpdh-1(ok1558) , and gpdh-2(ok1733) animals failing to arrest development at 500 mM NaCl after 48 hrs. Error bars, s.d. n = 3 experiments of > 100 animals (c) Average fold change of 2 replicates of the 25 most upregulated genes in embryos in response to osmotic stress after 6 hrs. (d) Percent of wild-type, daf-2(e1370) and gpdh-2(ok1733) cross progeny failing to arrest development after 48 hrs at 500 mM NaCl. Males contained otIs39 (Punc-47::GFP); him-5(e1490) for the identification of cross progeny. Error bars, s.d. n = 3 experiments of > 20 animals. The quantified results are presented as mean ± s.d. using ANOVA. * P
Figure Legend Snippet: Insulin-like signalling to the maternal germline regulates progeny response to osmotic stress (a) Percent of wild-type, daf-2(e1370) and daf-2(e1370); daf-16(mu86) cross progeny failing to arrest development after 48 hrs at 500 mM NaCl. Males contained (Pegl-1:: 4xNLS::GFP ); him-5(e1490); nIs349 (Pceh-28:: 4xNLS::mCherry) for the identification of cross progeny. The pie-1 promoter was used to drive germline specific expression of DAF-2 and the mex-5 promoter was used to drive germline specific expression of DAF-16. Error bars, s.d. n = 7, 3, 6, 3, 3, 3, and 3 see Supplementary Table 6 . (b) Percent of wild-type, gpdh-1(ok1558) , and gpdh-2(ok1733) animals failing to arrest development at 500 mM NaCl after 48 hrs. Error bars, s.d. n = 3 experiments of > 100 animals (c) Average fold change of 2 replicates of the 25 most upregulated genes in embryos in response to osmotic stress after 6 hrs. (d) Percent of wild-type, daf-2(e1370) and gpdh-2(ok1733) cross progeny failing to arrest development after 48 hrs at 500 mM NaCl. Males contained otIs39 (Punc-47::GFP); him-5(e1490) for the identification of cross progeny. Error bars, s.d. n = 3 experiments of > 20 animals. The quantified results are presented as mean ± s.d. using ANOVA. * P

Techniques Used: Expressing

Related Articles

Staining:

Article Title: Astrocyte- and Neuron-Derived CXCL1 Drives Neutrophil Transmigration and Blood-Brain Barrier Permeability in Viral Encephalitis
Article Snippet: .. Samples of stained cortex were then washed in PAB, placed on a microscope slide and imaged using a wide-field fluorescence microscope and monochrome CCD digital camera and analyzed using Zen Blue software (Zeiss, Oberkochen, Germany). .. In vitro infection of primary cells Primary neurons (#M1520), microglia (#M1900) and astrocytes (#M1820) isolated from CD1 mice where purchased from ScienCell Research Laboratories (Carlsbad, CA).

Expressing:

Article Title: Regulation and function of macrophage colony-stimulating factor (CSF1) in the chicken immune system
Article Snippet: .. To examine the transgene expression in major lymphoid tissues including cecal tonsil, bursa and spleen, the mean fluorescence intensity (MFI) and surface area was obtained from whole mount images analysis using Zeiss blue software. ..

Fluorescence:

Article Title: Regulation and function of macrophage colony-stimulating factor (CSF1) in the chicken immune system
Article Snippet: .. To examine the transgene expression in major lymphoid tissues including cecal tonsil, bursa and spleen, the mean fluorescence intensity (MFI) and surface area was obtained from whole mount images analysis using Zeiss blue software. ..

Article Title: Astrocyte- and Neuron-Derived CXCL1 Drives Neutrophil Transmigration and Blood-Brain Barrier Permeability in Viral Encephalitis
Article Snippet: .. Samples of stained cortex were then washed in PAB, placed on a microscope slide and imaged using a wide-field fluorescence microscope and monochrome CCD digital camera and analyzed using Zen Blue software (Zeiss, Oberkochen, Germany). .. In vitro infection of primary cells Primary neurons (#M1520), microglia (#M1900) and astrocytes (#M1820) isolated from CD1 mice where purchased from ScienCell Research Laboratories (Carlsbad, CA).

Microscopy:

Article Title: Characterization of orally efficacious influenza drug with high resistance barrier in ferrets and human airway epithelia
Article Snippet: .. A Zeiss LSM 800 confocal microscope coupled with AiryScan module was used for detection, Zeiss Zen Blue software was employed for image analyses. .. Influenza infection and NHC treatment in airway 3D ALI model 3D human airway epithelial cells were apically infected with influenza A or B viruses (5,000 pfu/transwell) for 2 hours, followed by washing of the apical chamber 3-times with media.

Article Title: Chlamydia trachomatis Oligopeptide Transporter Performs Dual Functions of Oligopeptide Transport and Peptidoglycan Recycling
Article Snippet: .. Each coverslip was photographed under a ×400 magnification using a Z1 Axiovert Observer epifluorescence microscope and the accompanying Zen Blue software (Zeiss, Oberkochen, Germany). ..

Article Title: Astrocyte- and Neuron-Derived CXCL1 Drives Neutrophil Transmigration and Blood-Brain Barrier Permeability in Viral Encephalitis
Article Snippet: .. Samples of stained cortex were then washed in PAB, placed on a microscope slide and imaged using a wide-field fluorescence microscope and monochrome CCD digital camera and analyzed using Zen Blue software (Zeiss, Oberkochen, Germany). .. In vitro infection of primary cells Primary neurons (#M1520), microglia (#M1900) and astrocytes (#M1820) isolated from CD1 mice where purchased from ScienCell Research Laboratories (Carlsbad, CA).

Software:

Article Title: DNA Methylation Enzymes and PRC1 Restrict B-cell Epstein-Barr Virus Oncoprotein Expression
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Article Title: Neutrophil extracellular traps target senescent vasculature for tissue remodeling in retinopathy.
Article Snippet: .. In developed countries, the leading causes of blindness such as diabetic retinopathy are characterized by disorganized vasculature that can become fibrotic. .. In developed countries, the leading causes of blindness such as diabetic retinopathy are characterized by disorganized vasculature that can become fibrotic.

Article Title: Regulation and function of macrophage colony-stimulating factor (CSF1) in the chicken immune system
Article Snippet: .. To examine the transgene expression in major lymphoid tissues including cecal tonsil, bursa and spleen, the mean fluorescence intensity (MFI) and surface area was obtained from whole mount images analysis using Zeiss blue software. ..

Article Title: Characterization of orally efficacious influenza drug with high resistance barrier in ferrets and human airway epithelia
Article Snippet: .. A Zeiss LSM 800 confocal microscope coupled with AiryScan module was used for detection, Zeiss Zen Blue software was employed for image analyses. .. Influenza infection and NHC treatment in airway 3D ALI model 3D human airway epithelial cells were apically infected with influenza A or B viruses (5,000 pfu/transwell) for 2 hours, followed by washing of the apical chamber 3-times with media.

Article Title: Chlamydia trachomatis Oligopeptide Transporter Performs Dual Functions of Oligopeptide Transport and Peptidoglycan Recycling
Article Snippet: .. Each coverslip was photographed under a ×400 magnification using a Z1 Axiovert Observer epifluorescence microscope and the accompanying Zen Blue software (Zeiss, Oberkochen, Germany). ..

Article Title: Mechanism of pulmonary immunosuppression: extrapulmonary burn injury suppresses bacterial endotoxin–induced pulmonary neutrophil recruitment and neutrophil extracellular trap (NET) formation
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Article Title: Astrocyte- and Neuron-Derived CXCL1 Drives Neutrophil Transmigration and Blood-Brain Barrier Permeability in Viral Encephalitis
Article Snippet: .. Samples of stained cortex were then washed in PAB, placed on a microscope slide and imaged using a wide-field fluorescence microscope and monochrome CCD digital camera and analyzed using Zen Blue software (Zeiss, Oberkochen, Germany). .. In vitro infection of primary cells Primary neurons (#M1520), microglia (#M1900) and astrocytes (#M1820) isolated from CD1 mice where purchased from ScienCell Research Laboratories (Carlsbad, CA).

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Article Snippet: .. Acquisition of the images was performed using Zen black or blue software (Carl Zeiss). ..

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