lr disruptors filipin  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 85

    Structured Review

    Millipore lr disruptors filipin
    Effects of Oxo on ASMase and ADP-ribosylcyclase activity in CAMs in the absence or presence of LR disruptors. A : summarized ASMase activity of CAMs treated with Oxo (80 μM, 15 min) alone or with 20-min pretreatment of MCD (100 μM), <t>filipin</t>
    Lr Disruptors Filipin, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lr disruptors filipin/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lr disruptors filipin - by Bioz Stars, 2020-04
    85/100 stars

    Related Products / Commonly Used Together

    methyl-β-cyclodextrin
    asmase inhibitor desipramine

    Images

    1) Product Images from "Formation and function of ceramide-enriched membrane platforms with CD38 during M1-receptor stimulation in bovine coronary arterial myocytes"

    Article Title: Formation and function of ceramide-enriched membrane platforms with CD38 during M1-receptor stimulation in bovine coronary arterial myocytes

    Journal:

    doi: 10.1152/ajpheart.00617.2008

    Effects of Oxo on ASMase and ADP-ribosylcyclase activity in CAMs in the absence or presence of LR disruptors. A : summarized ASMase activity of CAMs treated with Oxo (80 μM, 15 min) alone or with 20-min pretreatment of MCD (100 μM), filipin
    Figure Legend Snippet: Effects of Oxo on ASMase and ADP-ribosylcyclase activity in CAMs in the absence or presence of LR disruptors. A : summarized ASMase activity of CAMs treated with Oxo (80 μM, 15 min) alone or with 20-min pretreatment of MCD (100 μM), filipin

    Techniques Used: Activity Assay

    Western blot analysis of CD38 and ASMase in LR fractions isolated from CAMs stimulated by Oxo. A : CAMs were treated with Oxo (80 μM, 15 min) alone or with 20-min pretreatment of MCD (100 μM), filipin (1 μg), or Des (10 μM).
    Figure Legend Snippet: Western blot analysis of CD38 and ASMase in LR fractions isolated from CAMs stimulated by Oxo. A : CAMs were treated with Oxo (80 μM, 15 min) alone or with 20-min pretreatment of MCD (100 μM), filipin (1 μg), or Des (10 μM).

    Techniques Used: Western Blot, Isolation

    Related Articles

    Fluorescence:

    Article Title: Formation and function of ceramide-enriched membrane platforms with CD38 during M1-receptor stimulation in bovine coronary arterial myocytes
    Article Snippet: Individual LRs are too small (∼50 nm in diameter) to be resolved by standard light microscopy; however, if LR components are cross-linked in living cells, clustered raft protein and lipid components can be visualized by fluorescence microscopy. .. In additional groups of cells, LR disruptors filipin (1 μg; Sigma) or methyl-β-cyclodextrin (MCD; 100 μM; Sigma) or an ASMase inhibitor desipramine (Des, 10 μM; Sigma) were added and incubated for 20 min before Oxo stimulation.

    Microscopy:

    Article Title: Formation and function of ceramide-enriched membrane platforms with CD38 during M1-receptor stimulation in bovine coronary arterial myocytes
    Article Snippet: Individual LRs are too small (∼50 nm in diameter) to be resolved by standard light microscopy; however, if LR components are cross-linked in living cells, clustered raft protein and lipid components can be visualized by fluorescence microscopy. .. In additional groups of cells, LR disruptors filipin (1 μg; Sigma) or methyl-β-cyclodextrin (MCD; 100 μM; Sigma) or an ASMase inhibitor desipramine (Des, 10 μM; Sigma) were added and incubated for 20 min before Oxo stimulation.

    Light Microscopy:

    Article Title: Formation and function of ceramide-enriched membrane platforms with CD38 during M1-receptor stimulation in bovine coronary arterial myocytes
    Article Snippet: Individual LRs are too small (∼50 nm in diameter) to be resolved by standard light microscopy; however, if LR components are cross-linked in living cells, clustered raft protein and lipid components can be visualized by fluorescence microscopy. .. In additional groups of cells, LR disruptors filipin (1 μg; Sigma) or methyl-β-cyclodextrin (MCD; 100 μM; Sigma) or an ASMase inhibitor desipramine (Des, 10 μM; Sigma) were added and incubated for 20 min before Oxo stimulation.

    Incubation:

    Article Title: Formation and function of ceramide-enriched membrane platforms with CD38 during M1-receptor stimulation in bovine coronary arterial myocytes
    Article Snippet: .. In additional groups of cells, LR disruptors filipin (1 μg; Sigma) or methyl-β-cyclodextrin (MCD; 100 μM; Sigma) or an ASMase inhibitor desipramine (Des, 10 μM; Sigma) were added and incubated for 20 min before Oxo stimulation. .. These cells were then washed in cold PBS and fixed for 10 min in 4% paraformaldehyde and blocked with 1% BSA in TBS for 30 min. GM1 gangliosides enriched in LRs were stained by Alexa488-labeled cholera toxin B (Alexa488-CTXB, 1 μg/ml) (Molecular Probes, Eugene, OR).

    Staining:

    Article Title: Formation and function of ceramide-enriched membrane platforms with CD38 during M1-receptor stimulation in bovine coronary arterial myocytes
    Article Snippet: In additional groups of cells, LR disruptors filipin (1 μg; Sigma) or methyl-β-cyclodextrin (MCD; 100 μM; Sigma) or an ASMase inhibitor desipramine (Des, 10 μM; Sigma) were added and incubated for 20 min before Oxo stimulation. .. These cells were then washed in cold PBS and fixed for 10 min in 4% paraformaldehyde and blocked with 1% BSA in TBS for 30 min. GM1 gangliosides enriched in LRs were stained by Alexa488-labeled cholera toxin B (Alexa488-CTXB, 1 μg/ml) (Molecular Probes, Eugene, OR).

    Plasmid Preparation:

    Article Title: Formation and function of ceramide-enriched membrane platforms with CD38 during M1-receptor stimulation in bovine coronary arterial myocytes
    Article Snippet: In additional groups of cells, LR disruptors filipin (1 μg; Sigma) or methyl-β-cyclodextrin (MCD; 100 μM; Sigma) or an ASMase inhibitor desipramine (Des, 10 μM; Sigma) were added and incubated for 20 min before Oxo stimulation. .. The stained cells were then extensively washed, fixed in 4% paraformaldehyde for another 10 min, and mounted on glass slide with Vectashield mounting media (Vector, Burlingame, CA).