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Upstate Biotechnology Inc low salt immune complex wash buffer
Low Salt Immune Complex Wash Buffer, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/low salt immune complex wash buffer/product/Upstate Biotechnology Inc
Average 88 stars, based on 3 article reviews
Price from $9.99 to $1999.99
low salt immune complex wash buffer - by Bioz Stars, 2020-07
88/100 stars

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Incubation:

Article Title: Role of Glucocorticoids and cAMP-Mediated Repression in Limiting Corticotropin-Releasing Hormone Transcription during Stress
Article Snippet: .. After overnight incubation, 65 μl of salmon-sperm DNA/protein A-agarose slurry was added and incubated for 1 h at 4°C under rotation, and DNA-protein complexes were separated by centrifugation at 100 × g for 3 min. Pellets were washed sequentially in low-salt immune-complex wash buffer, high-salt immune-complex wash buffer, and LiCl immune-complex wash buffer (Upstate Biotechnology) for 3-5 min, followed by two washes in Tris-EDTA buffer and elution twice with 250 μl of 1% SDS in 0.1 m NaHCO3 . .. Aliquots of eluate pools and input chromatin were incubated at 65°C for 4-18 h in 0.2 m NaCl to reverse cross-linking and then incubated at 45°C for 1 h in 40 μ m Tris-HCl, pH 6.5, containing 10 μ m EDTA and 20 μg of proteinase K to remove proteins.

Centrifugation:

Article Title: Role of Glucocorticoids and cAMP-Mediated Repression in Limiting Corticotropin-Releasing Hormone Transcription during Stress
Article Snippet: .. After overnight incubation, 65 μl of salmon-sperm DNA/protein A-agarose slurry was added and incubated for 1 h at 4°C under rotation, and DNA-protein complexes were separated by centrifugation at 100 × g for 3 min. Pellets were washed sequentially in low-salt immune-complex wash buffer, high-salt immune-complex wash buffer, and LiCl immune-complex wash buffer (Upstate Biotechnology) for 3-5 min, followed by two washes in Tris-EDTA buffer and elution twice with 250 μl of 1% SDS in 0.1 m NaHCO3 . .. Aliquots of eluate pools and input chromatin were incubated at 65°C for 4-18 h in 0.2 m NaCl to reverse cross-linking and then incubated at 45°C for 1 h in 40 μ m Tris-HCl, pH 6.5, containing 10 μ m EDTA and 20 μg of proteinase K to remove proteins.

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  • 88
    Upstate Biotechnology Inc low salt immune complex wash buffer
    Low Salt Immune Complex Wash Buffer, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/low salt immune complex wash buffer/product/Upstate Biotechnology Inc
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    low salt immune complex wash buffer - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    85
    Upstate Biotechnology Inc licl immune complex wash buffers
    Licl Immune Complex Wash Buffers, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/licl immune complex wash buffers/product/Upstate Biotechnology Inc
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    licl immune complex wash buffers - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

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