low molecular weight lmw dna ladders  (New England Biolabs)


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    Name:
    Low Molecular Weight DNA Ladder
    Description:
    Low Molecular Weight DNA Ladder 500 gel lanes
    Catalog Number:
    N3233L
    Price:
    270
    Category:
    DNA Ladders
    Size:
    500 gel lanes
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    Structured Review

    New England Biolabs low molecular weight lmw dna ladders
    Low Molecular Weight DNA Ladder
    Low Molecular Weight DNA Ladder 500 gel lanes
    https://www.bioz.com/result/low molecular weight lmw dna ladders/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    low molecular weight lmw dna ladders - by Bioz Stars, 2021-05
    99/100 stars

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    Related Articles

    Molecular Weight:

    Article Title: RIPiT-Seq: A high-throughput approach for footprinting RNA:protein complexes
    Article Snippet: To estimate the size-range and molar amounts of the purified RNA, we also 5′-end label oligonucleotide of known size and concentration and run them on the same gel. .. In separate, but otherwise identical reactions, also label 0.1 pmole of a synthetic RNA oligonucleotide of known length, 1 µl of low molecular weight ssDNA ladder and 1 µl of NEB 100 bp DNA ladder. ..

    Article Title: Optical mapping reveals a higher level of genomic architecture of chained fusions in cancer
    Article Snippet: The DNA was left to equilibrate for 4 d, then quantified using the Qubit Broad Range dsDNA Assay Kit (Thermo Fisher Scientific). .. Using the IrysPrep NLRS assay (Bionano Genomics), 200–300 ng/µL of high molecular weight DNA underwent single-strand nicking with 10 units of Nt.BspQI nickase (New England BioLabs). ..

    Article Title: Plasmid replication-associated single-strand-specific methyltransferases
    Article Snippet: Fragments were separated on appropriate agarose or 20% PAGE gels in TBE buffer, transferred to HybondTM -N+ (Amersham, NJ) positively charged membranes, sprayed with EN3HANCER (Perkin Elmer) and visualized with ECL Hyperfilm™ (Amersham, NJ). .. DNA markers for fluorography were made by modification of NEB N3012 (Lambda HindIII digest) or NEB N3233 (Low Molecular Weight DNA Ladder) with the nonspecific A-DNA MTase, M.EcoGII (NEB M0603). .. DNA markers for fluorography were made by modification of NEB N3012 (Lambda HindIII digest) or NEB N3233 (Low Molecular Weight DNA Ladder) with the nonspecific A-DNA MTase, M.EcoGII (NEB M0603).

    Article Title: Structural diversity of supercoiled DNA
    Article Snippet: Acrylamide, ampicillin, chloroform, isopropyl beta-D-thiogalactoside, sodium chloride and sodium citrate were purchased from Fisher Scientific (Pittsburgh, PA). .. BbvCI, EcoRV, Nb.BbvCI, NdeI, Nuclease Bal-31, T4 DNA Ligase, low molecular weight DNA ladder and 100 bp DNA ladder were purchased from New England Biolabs (Ipswich, MA). .. Proteinase K was purchased from Roche Molecular Biochemicals (Mannheim, Germany).

    Article Title: Double Stranded Sperm DNA Breaks, Measured by Comet Assay, Are Associated with Unexplained Recurrent Miscarriage in Couples without a Female Factor
    Article Snippet: A quarter slice of each plug was cut off and placed on 1% gel and resolved by electrophoresis on a contour-clamped homogeneous electric field apparatus (Bio-Rad CHEF DRIII system) in TBE 0.5 X (Tris-borate 50 mM, EDTA 0.1 mM) at a 120° angle, 14°C, 4 V/cm and with the following pulses: 6.7 tp 33.7 seconds for 27.1 hours. .. DNA molecular weight markers consisting of Lambda ladder PFG marker and Low Range PFG Marker (New England Biolabs; Ipswich, MA, USA) were included in each electrophoretic run. .. Gels were stained with ethidium bromide and visualised and photographed under ultraviolet light using the GelDoc System (BioRad; Hercules, CA, USA).

    Article Title: Repeat-induced gene silencing of L1 transgenes is correlated with differential promoter methylation
    Article Snippet: .. Control tail gDNA samples L-high, LSL and L, detailed in text; wt, wild-type C57BL/6 tail gDNA sample; NTC, no template control; M, 100 bp DNA molecular weight ladder (New England Biolabs). (C) Calibration of retrotransposition qPCR with mouse tail gDNA and plasmid DNA samples. .. The presence/absence of the high-copy donor transgene and the number of germline insertions were examined independently by Southern blot (panel D).

    Article Title: Intra-tumor heterogeneity of MLH1 promoter methylation revealed by deep single molecule bisulfite sequencing
    Article Snippet: One-fifth of the PCR reaction for each of 66 reactions was quantified by electrophoresis on a 2% agarose gel. .. This was achieved by quantitatively comparing the intensity of the gel band containing the PCR product to the intensity of the similar size band in the Low Molecular Weight DNA Ladder (NEB). .. The quantity of PCR product is computed by dividing the PCR product intensity by the scaling factor, which is the ladder band intensity divided by the ladder band molecular weight.

    Article Title: Nisin H Is a New Nisin Variant Produced by the Gut-Derived Strain Streptococcus hyointestinalis DPC6484
    Article Snippet: .. Molecular fingerprinting of S. hyointestinalis isolates was performed by pulsed-field gel electrophoresis (PFGE) as described by Simpson et al. (2002) ( ) using SmaI restriction endonucleases and DNA molecular weight markers (9.42 to 242.50 kb; New England BioLabs, Beverly, MA). .. DNA fragments were resolved with a CHEF (contour-clamped homogeneous electric field) DRIII pulsed-field system (Bio-Rad Laboratories) at 6 V/cm for 18 h with a 1- to 30-s linear ramp time to resolve bands.

    Modification:

    Article Title: Plasmid replication-associated single-strand-specific methyltransferases
    Article Snippet: Fragments were separated on appropriate agarose or 20% PAGE gels in TBE buffer, transferred to HybondTM -N+ (Amersham, NJ) positively charged membranes, sprayed with EN3HANCER (Perkin Elmer) and visualized with ECL Hyperfilm™ (Amersham, NJ). .. DNA markers for fluorography were made by modification of NEB N3012 (Lambda HindIII digest) or NEB N3233 (Low Molecular Weight DNA Ladder) with the nonspecific A-DNA MTase, M.EcoGII (NEB M0603). .. DNA markers for fluorography were made by modification of NEB N3012 (Lambda HindIII digest) or NEB N3233 (Low Molecular Weight DNA Ladder) with the nonspecific A-DNA MTase, M.EcoGII (NEB M0603).

    Marker:

    Article Title: Double Stranded Sperm DNA Breaks, Measured by Comet Assay, Are Associated with Unexplained Recurrent Miscarriage in Couples without a Female Factor
    Article Snippet: A quarter slice of each plug was cut off and placed on 1% gel and resolved by electrophoresis on a contour-clamped homogeneous electric field apparatus (Bio-Rad CHEF DRIII system) in TBE 0.5 X (Tris-borate 50 mM, EDTA 0.1 mM) at a 120° angle, 14°C, 4 V/cm and with the following pulses: 6.7 tp 33.7 seconds for 27.1 hours. .. DNA molecular weight markers consisting of Lambda ladder PFG marker and Low Range PFG Marker (New England Biolabs; Ipswich, MA, USA) were included in each electrophoretic run. .. Gels were stained with ethidium bromide and visualised and photographed under ultraviolet light using the GelDoc System (BioRad; Hercules, CA, USA).

    Real-time Polymerase Chain Reaction:

    Article Title: Repeat-induced gene silencing of L1 transgenes is correlated with differential promoter methylation
    Article Snippet: .. Control tail gDNA samples L-high, LSL and L, detailed in text; wt, wild-type C57BL/6 tail gDNA sample; NTC, no template control; M, 100 bp DNA molecular weight ladder (New England Biolabs). (C) Calibration of retrotransposition qPCR with mouse tail gDNA and plasmid DNA samples. .. The presence/absence of the high-copy donor transgene and the number of germline insertions were examined independently by Southern blot (panel D).

    Plasmid Preparation:

    Article Title: Repeat-induced gene silencing of L1 transgenes is correlated with differential promoter methylation
    Article Snippet: .. Control tail gDNA samples L-high, LSL and L, detailed in text; wt, wild-type C57BL/6 tail gDNA sample; NTC, no template control; M, 100 bp DNA molecular weight ladder (New England Biolabs). (C) Calibration of retrotransposition qPCR with mouse tail gDNA and plasmid DNA samples. .. The presence/absence of the high-copy donor transgene and the number of germline insertions were examined independently by Southern blot (panel D).

    Polymerase Chain Reaction:

    Article Title: Intra-tumor heterogeneity of MLH1 promoter methylation revealed by deep single molecule bisulfite sequencing
    Article Snippet: One-fifth of the PCR reaction for each of 66 reactions was quantified by electrophoresis on a 2% agarose gel. .. This was achieved by quantitatively comparing the intensity of the gel band containing the PCR product to the intensity of the similar size band in the Low Molecular Weight DNA Ladder (NEB). .. The quantity of PCR product is computed by dividing the PCR product intensity by the scaling factor, which is the ladder band intensity divided by the ladder band molecular weight.

    Pulsed-Field Gel:

    Article Title: Nisin H Is a New Nisin Variant Produced by the Gut-Derived Strain Streptococcus hyointestinalis DPC6484
    Article Snippet: .. Molecular fingerprinting of S. hyointestinalis isolates was performed by pulsed-field gel electrophoresis (PFGE) as described by Simpson et al. (2002) ( ) using SmaI restriction endonucleases and DNA molecular weight markers (9.42 to 242.50 kb; New England BioLabs, Beverly, MA). .. DNA fragments were resolved with a CHEF (contour-clamped homogeneous electric field) DRIII pulsed-field system (Bio-Rad Laboratories) at 6 V/cm for 18 h with a 1- to 30-s linear ramp time to resolve bands.

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  • 99
    New England Biolabs lmw dna ladder
    Lmw Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lmw dna ladder/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lmw dna ladder - by Bioz Stars, 2021-05
    99/100 stars
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