Structured Review

Luminex jev
<t>ELISA</t> assays for comparison with the developed biosensor: (a) Indirect binding ELISA and specificity assay for <t>JEV,</t> (b) Competitive ELISA and specificity assay for JEV, (c) Indirect binding ELISA and specificity assay for AIV, (d) Competitive ELISA and specificity assay for AIV.
Jev, supplied by Luminex, used in various techniques. Bioz Stars score: 92/100, based on 102 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/jev/product/Luminex
Average 92 stars, based on 102 article reviews
Price from $9.99 to $1999.99
jev - by Bioz Stars, 2020-12
92/100 stars

Images

1) Product Images from "Graphene functionalized field-effect transistors for ultrasensitive detection of Japanese encephalitis and Avian influenza virus"

Article Title: Graphene functionalized field-effect transistors for ultrasensitive detection of Japanese encephalitis and Avian influenza virus

Journal: Scientific Reports

doi: 10.1038/s41598-020-71591-w

ELISA assays for comparison with the developed biosensor: (a) Indirect binding ELISA and specificity assay for JEV, (b) Competitive ELISA and specificity assay for JEV, (c) Indirect binding ELISA and specificity assay for AIV, (d) Competitive ELISA and specificity assay for AIV.
Figure Legend Snippet: ELISA assays for comparison with the developed biosensor: (a) Indirect binding ELISA and specificity assay for JEV, (b) Competitive ELISA and specificity assay for JEV, (c) Indirect binding ELISA and specificity assay for AIV, (d) Competitive ELISA and specificity assay for AIV.

Techniques Used: Enzyme-linked Immunosorbent Assay, Binding Assay, Competitive ELISA

2) Product Images from "Detection of chikungunya viral RNA in mosquito bodies on cationic (Q) paper based on innovations in synthetic biology"

Article Title: Detection of chikungunya viral RNA in mosquito bodies on cationic (Q) paper based on innovations in synthetic biology

Journal: Journal of virological methods

doi: 10.1016/j.jviromet.2017.04.013

Luminex DHA profiles executed with PCR–amplicons of CHIKV RNA eluates from Q-paper. The CHIKV pure RNA solution was serially diluted, and each RNA dilution was loaded on the top of Q-paper. RNA copies in each load are given on the top of each bar. The RNA were eluted by high salt solution (150 μL of 1M NaCl), column-desalted, collected in 50 μL of water, and 5 μL aliquots of each sample was PCR – amplified. The numbers on the bars’ bottom are the RNA copies in the eluates’ aliquots taken into RT-PCRs. They are counted from the consideration that elution efficiency was 100% and RNA were intact. Sim, CHIKV, 85 base pairs amplicon, in vitro produced RNA fragment simulated viral RNA (Luminex DHA positive control). Each sample was run in triplicate. MFI, median fluorescence intensities.
Figure Legend Snippet: Luminex DHA profiles executed with PCR–amplicons of CHIKV RNA eluates from Q-paper. The CHIKV pure RNA solution was serially diluted, and each RNA dilution was loaded on the top of Q-paper. RNA copies in each load are given on the top of each bar. The RNA were eluted by high salt solution (150 μL of 1M NaCl), column-desalted, collected in 50 μL of water, and 5 μL aliquots of each sample was PCR – amplified. The numbers on the bars’ bottom are the RNA copies in the eluates’ aliquots taken into RT-PCRs. They are counted from the consideration that elution efficiency was 100% and RNA were intact. Sim, CHIKV, 85 base pairs amplicon, in vitro produced RNA fragment simulated viral RNA (Luminex DHA positive control). Each sample was run in triplicate. MFI, median fluorescence intensities.

Techniques Used: Luminex, Polymerase Chain Reaction, Amplification, In Vitro, Produced, Positive Control, Fluorescence

Related Articles

Multiplexing:

Article Title: The function of cancer-shed gangliosides in macrophage phenotype: involvement with angiogenesis
Article Snippet: .. Interestingly, as evidenced by RT-PCR and Luminex multiplexing assay (Figure ), MCP-1 expression was clearly increased by GM1 via the CD206/JAK3-mediated pathway in macrophage cells, similar to the regulation of Arg-1 expression by GM1 (Figure ). .. Furthermore, MCP-1 released from macrophages stimulated by GM1 induced the activation of endothelial cells, which was inhibited by a CCR2 antagonist (Figures and ).

Fluorescence:

Article Title: Design and Evaluation of a Multiplexed Assay to Assess Human Immunogenicity Against Humira®
Article Snippet: .. Finally, the Luminex FLEXMAP 3D instrument utilizes the fluorescence of phycoerythrin to generate the assay signal. ..

Polymerase Chain Reaction:

Article Title: High-throughput identification of genotype-specific cancer vulnerabilities in mixtures of barcoded tumor cell lines
Article Snippet: .. PCR product was hybridized to Luminex beads with covalently attached antisense barcodes, and streptavidin-phycoerythrin addition, washing, and detection on Luminex FlexMap machines was performed as previously described . ..

other:

Article Title: A Multiplex Ligase Detection Reaction-Fluorescent Microsphere Assay for Simultaneous Detection of Single Nucleotide Polymorphisms Associated with Plasmodium falciparum Drug Resistance ▿
Article Snippet: We thank S. A. Dunbar for a helpful discussion regarding Luminex FlexMAP microsphere sets.

Expressing:

Article Title: The function of cancer-shed gangliosides in macrophage phenotype: involvement with angiogenesis
Article Snippet: .. Interestingly, as evidenced by RT-PCR and Luminex multiplexing assay (Figure ), MCP-1 expression was clearly increased by GM1 via the CD206/JAK3-mediated pathway in macrophage cells, similar to the regulation of Arg-1 expression by GM1 (Figure ). .. Furthermore, MCP-1 released from macrophages stimulated by GM1 induced the activation of endothelial cells, which was inhibited by a CCR2 antagonist (Figures and ).

Reverse Transcription Polymerase Chain Reaction:

Article Title: The function of cancer-shed gangliosides in macrophage phenotype: involvement with angiogenesis
Article Snippet: .. Interestingly, as evidenced by RT-PCR and Luminex multiplexing assay (Figure ), MCP-1 expression was clearly increased by GM1 via the CD206/JAK3-mediated pathway in macrophage cells, similar to the regulation of Arg-1 expression by GM1 (Figure ). .. Furthermore, MCP-1 released from macrophages stimulated by GM1 induced the activation of endothelial cells, which was inhibited by a CCR2 antagonist (Figures and ).

Software:

Article Title: Development of a five-plex flow cytometric immunoassay for the simultaneous detection of six coccidiostats in feed and eggs
Article Snippet: .. The FLEXMAP 3D® system, controlled with xPONENT 4.0 Software Solutions, was obtained from Luminex (Austin, Texas, USA). .. All washing steps were carried out on a Bio-Plex™ Pro II Wash Station (Bio-Rad Laboratories, Veenendaal, The Netherlands) using a magnetic plate carrier or the vacuum carrier.

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