loop mediated isothermal amplification lamp  (Bio-Rad)

 
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    Name:
    Lamp
    Description:
    Pkg of 1 replacement lamp for Model EM 1 Econo UV monitor 731 8160
    Catalog Number:
    7318166
    Price:
    None
    Category:
    Chromatography Systems Components and Accessories
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    Bio-Rad loop mediated isothermal amplification lamp
    Lamp
    Pkg of 1 replacement lamp for Model EM 1 Econo UV monitor 731 8160
    https://www.bioz.com/result/loop mediated isothermal amplification lamp/product/Bio-Rad
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    loop mediated isothermal amplification lamp - by Bioz Stars, 2020-08
    94/100 stars

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    Related Articles

    Produced:

    Article Title: Nickel Stimulates L1 Retrotransposition by a Post-transcriptional Mechanism
    Article Snippet: .. The RNA was cross-linked to the membrane using a UV lamp (GS Gene linker, BioRad) and pre-hybridized in 30% (v/v) formamide, Denhardt’s solution, 1% (w/v) SDS, 1 M NaCl, 100 μg/ml of salmon sperm DNA, 100 μg/ml of yeast tRNA at 60 °C for at least 6 h. DNA template for the probe was produced by PCR with the primers that amplified the 3′ region of the neomycin gene (forward: 5′-CGACCCAACACC-CGTGCG-3′ ; reverse: 5″-AGGACGAGGCAGCGCGGC-3′. .. PCR products were fractionated on a 1% low-melting agarose gel, excised, and purified using a QIAquick gel extraction kit (QIAGEN).

    Amplification:

    Article Title: Nickel Stimulates L1 Retrotransposition by a Post-transcriptional Mechanism
    Article Snippet: .. The RNA was cross-linked to the membrane using a UV lamp (GS Gene linker, BioRad) and pre-hybridized in 30% (v/v) formamide, Denhardt’s solution, 1% (w/v) SDS, 1 M NaCl, 100 μg/ml of salmon sperm DNA, 100 μg/ml of yeast tRNA at 60 °C for at least 6 h. DNA template for the probe was produced by PCR with the primers that amplified the 3′ region of the neomycin gene (forward: 5′-CGACCCAACACC-CGTGCG-3′ ; reverse: 5″-AGGACGAGGCAGCGCGGC-3′. .. PCR products were fractionated on a 1% low-melting agarose gel, excised, and purified using a QIAquick gel extraction kit (QIAGEN).

    Agarose Gel Electrophoresis:

    Article Title: Aromatic Modification of Low Molecular Weight PEI for Enhanced Gene Delivery
    Article Snippet: .. Then the mixture was electrophoresed on a 1% (w /v ) agarose gel containing GelRedTM in a Triseacetate (TAE) running buffer at 120 V for 40 min. Then DNA was visualized under an ultraviolet lamp using a BioRad Universal Hood II. .. Fluorescence Spectroscopy Fluorescence spectra were measured at room temperature in air by a HITACHI F-7000 Fluorescence Spectrophotometer (Hitachi High-Tech Science, Schaumburg, IL, USA) and corrected for the system response.

    Article Title: Biodegradable Gene Carriers Containing Rigid Aromatic Linkage with Enhanced DNA Binding and Cell Uptake
    Article Snippet: .. The obtained complex solution was diluted to a total volume of 10 μL, and then the complexes were incubated at 37 °C for 30 min. After addition of 2.5 μL of loading buffer, the complexes were electrophoresed on a 1% (w/v agarose gel containing GelRed™ and with Triseacetate (TAE) running buffer at 150 V for 30 min. DNA was visualized under an ultraviolet lamp using a BioRad Universal Hood II (Berkeley, CA, USA). ..

    Article Title: Cyclen-Based Cationic Lipids for Highly Efficient Gene Delivery towards Tumor Cells
    Article Snippet: .. 10 µL of each lipoplexes solution were electrophoresed on the 1% (W V-1) agarose gel containing Gold view and Tris-acetate (TAE) running buffer at 110 V for 30 min. DNA was visualized with a UV lamp using a BioRad Universal Hood II. .. Ethidium bromide intercalation assay Fluorescence emission due to ethidium bromide (EB) at 605 nm was monitored in a Fluoromax-4 spectrofluorimeter (Excitation wavelength was 497 nm with 3 nm slit widths for both excitation and emission).

    Article Title: Altered expression of metabotropic glutamate receptor 1 alpha after acute diffuse brain injury
    Article Snippet: .. The primer sequence is as follows: Five microliters of the product was analyzed in the following steps: electrophoresis on a 2% agarose gel containing the appropriate amount of ethidium bromide, ethidium bromide staining, observation under an ultraviolet lamp, images captured by a gel imaging system (Bio-Rad, Benicia, CA, USA), densitometry of the PCR product from the experimental group and the loading control GAPDH. .. The images were analyzed by Scion Image 4.02 (Scioncop, Betheda, MD, USA), and relative gene expression (absorbance of target gene segment/GAPDH gene segment) was calculated[ ].

    Electrophoresis:

    Article Title: Altered expression of metabotropic glutamate receptor 1 alpha after acute diffuse brain injury
    Article Snippet: .. The primer sequence is as follows: Five microliters of the product was analyzed in the following steps: electrophoresis on a 2% agarose gel containing the appropriate amount of ethidium bromide, ethidium bromide staining, observation under an ultraviolet lamp, images captured by a gel imaging system (Bio-Rad, Benicia, CA, USA), densitometry of the PCR product from the experimental group and the loading control GAPDH. .. The images were analyzed by Scion Image 4.02 (Scioncop, Betheda, MD, USA), and relative gene expression (absorbance of target gene segment/GAPDH gene segment) was calculated[ ].

    Polymerase Chain Reaction:

    Article Title: Nickel Stimulates L1 Retrotransposition by a Post-transcriptional Mechanism
    Article Snippet: .. The RNA was cross-linked to the membrane using a UV lamp (GS Gene linker, BioRad) and pre-hybridized in 30% (v/v) formamide, Denhardt’s solution, 1% (w/v) SDS, 1 M NaCl, 100 μg/ml of salmon sperm DNA, 100 μg/ml of yeast tRNA at 60 °C for at least 6 h. DNA template for the probe was produced by PCR with the primers that amplified the 3′ region of the neomycin gene (forward: 5′-CGACCCAACACC-CGTGCG-3′ ; reverse: 5″-AGGACGAGGCAGCGCGGC-3′. .. PCR products were fractionated on a 1% low-melting agarose gel, excised, and purified using a QIAquick gel extraction kit (QIAGEN).

    Article Title: Altered expression of metabotropic glutamate receptor 1 alpha after acute diffuse brain injury
    Article Snippet: .. The primer sequence is as follows: Five microliters of the product was analyzed in the following steps: electrophoresis on a 2% agarose gel containing the appropriate amount of ethidium bromide, ethidium bromide staining, observation under an ultraviolet lamp, images captured by a gel imaging system (Bio-Rad, Benicia, CA, USA), densitometry of the PCR product from the experimental group and the loading control GAPDH. .. The images were analyzed by Scion Image 4.02 (Scioncop, Betheda, MD, USA), and relative gene expression (absorbance of target gene segment/GAPDH gene segment) was calculated[ ].

    Incubation:

    Article Title: Biodegradable Gene Carriers Containing Rigid Aromatic Linkage with Enhanced DNA Binding and Cell Uptake
    Article Snippet: .. The obtained complex solution was diluted to a total volume of 10 μL, and then the complexes were incubated at 37 °C for 30 min. After addition of 2.5 μL of loading buffer, the complexes were electrophoresed on a 1% (w/v agarose gel containing GelRed™ and with Triseacetate (TAE) running buffer at 150 V for 30 min. DNA was visualized under an ultraviolet lamp using a BioRad Universal Hood II (Berkeley, CA, USA). ..

    Imaging:

    Article Title: Altered expression of metabotropic glutamate receptor 1 alpha after acute diffuse brain injury
    Article Snippet: .. The primer sequence is as follows: Five microliters of the product was analyzed in the following steps: electrophoresis on a 2% agarose gel containing the appropriate amount of ethidium bromide, ethidium bromide staining, observation under an ultraviolet lamp, images captured by a gel imaging system (Bio-Rad, Benicia, CA, USA), densitometry of the PCR product from the experimental group and the loading control GAPDH. .. The images were analyzed by Scion Image 4.02 (Scioncop, Betheda, MD, USA), and relative gene expression (absorbance of target gene segment/GAPDH gene segment) was calculated[ ].

    Sequencing:

    Article Title: Altered expression of metabotropic glutamate receptor 1 alpha after acute diffuse brain injury
    Article Snippet: .. The primer sequence is as follows: Five microliters of the product was analyzed in the following steps: electrophoresis on a 2% agarose gel containing the appropriate amount of ethidium bromide, ethidium bromide staining, observation under an ultraviolet lamp, images captured by a gel imaging system (Bio-Rad, Benicia, CA, USA), densitometry of the PCR product from the experimental group and the loading control GAPDH. .. The images were analyzed by Scion Image 4.02 (Scioncop, Betheda, MD, USA), and relative gene expression (absorbance of target gene segment/GAPDH gene segment) was calculated[ ].

    Staining:

    Article Title: Altered expression of metabotropic glutamate receptor 1 alpha after acute diffuse brain injury
    Article Snippet: .. The primer sequence is as follows: Five microliters of the product was analyzed in the following steps: electrophoresis on a 2% agarose gel containing the appropriate amount of ethidium bromide, ethidium bromide staining, observation under an ultraviolet lamp, images captured by a gel imaging system (Bio-Rad, Benicia, CA, USA), densitometry of the PCR product from the experimental group and the loading control GAPDH. .. The images were analyzed by Scion Image 4.02 (Scioncop, Betheda, MD, USA), and relative gene expression (absorbance of target gene segment/GAPDH gene segment) was calculated[ ].

    Hood:

    Article Title: Sunlight-Mediated Thiol–Ene/Yne Click Reaction: Synthesis and DNA Transfection Efficiency of New Cationic Lipids
    Article Snippet: .. The pDNA was visualized with a UV lamp using a BioRad Universal Hood. .. The gel retardation assay in the presence of serum was also performed following similar experimental methods.

    Article Title: Aromatic Modification of Low Molecular Weight PEI for Enhanced Gene Delivery
    Article Snippet: .. Then the mixture was electrophoresed on a 1% (w /v ) agarose gel containing GelRedTM in a Triseacetate (TAE) running buffer at 120 V for 40 min. Then DNA was visualized under an ultraviolet lamp using a BioRad Universal Hood II. .. Fluorescence Spectroscopy Fluorescence spectra were measured at room temperature in air by a HITACHI F-7000 Fluorescence Spectrophotometer (Hitachi High-Tech Science, Schaumburg, IL, USA) and corrected for the system response.

    Article Title: Biodegradable Gene Carriers Containing Rigid Aromatic Linkage with Enhanced DNA Binding and Cell Uptake
    Article Snippet: .. The obtained complex solution was diluted to a total volume of 10 μL, and then the complexes were incubated at 37 °C for 30 min. After addition of 2.5 μL of loading buffer, the complexes were electrophoresed on a 1% (w/v agarose gel containing GelRed™ and with Triseacetate (TAE) running buffer at 150 V for 30 min. DNA was visualized under an ultraviolet lamp using a BioRad Universal Hood II (Berkeley, CA, USA). ..

    Article Title: Cyclen-Based Cationic Lipids for Highly Efficient Gene Delivery towards Tumor Cells
    Article Snippet: .. 10 µL of each lipoplexes solution were electrophoresed on the 1% (W V-1) agarose gel containing Gold view and Tris-acetate (TAE) running buffer at 110 V for 30 min. DNA was visualized with a UV lamp using a BioRad Universal Hood II. .. Ethidium bromide intercalation assay Fluorescence emission due to ethidium bromide (EB) at 605 nm was monitored in a Fluoromax-4 spectrofluorimeter (Excitation wavelength was 497 nm with 3 nm slit widths for both excitation and emission).

    Software:

    Article Title: Effect of traditional Chinese medicine (Xiaochaihu Tang) on the expression of MMP-2 and MMP-9 in rats with endometriosis
    Article Snippet: .. The images were collected under an ultraviolet (UV) lamp and analyzed using Quantity One® 1-D Analysis Software v4.6 (Bio-Rad, Hercules, CA, USA). .. Western blotting The tissues of the eutopic and ectopic endometria were mixed using radio-immunoprecipitation assay (RIPA) lysis buffer at a volume ratio of 1:10.

    Article Title: Characterization of Human iPSC-RPE on a Prosthetic Bruch's Membrane Manufactured From Silk Fibroin
    Article Snippet: .. DNA was visualized by UV lamp and imaged using the ChemiDoc XRS system (Bio-Rad) and Quantity One software (Bio-Rad). .. Experimental Set-Up and Data Analyses All experiments were performed at least in triplicate on parallel, age-matched (days in culture) hiPSC-RPE cells grown on BMSF versus COL1-BMSF versus LAM-TCP similar tissue culture support (24-well plate versus Transwells and custom-made chambers).

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  • 94
    Bio-Rad loop mediated isothermal amplification lamp
    Loop Mediated Isothermal Amplification Lamp, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/loop mediated isothermal amplification lamp/product/Bio-Rad
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    loop mediated isothermal amplification lamp - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

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