long range polymerase chain reaction trusight hla v2 sequencing panel  (Illumina Inc)

 
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    Name:
    TruSight HLA v2 Sequencing Panel
    Description:
    The TruSight HLA v2 Sequencing Panel provides clinical researchers with a broad coverage ultra high resolution human leukocyte antigen HLA typing solution for simple rapid assessment of the HLA region in a single assay It features High resolution sequencing of 11 HLA loci Workflow with library preparation sequencing and data analysis reporting Accurate and high resolution HLA typing for unambiguous results Capture Full HLA Gene Sequences TruSight HLA v2 covers all commonly typed HLA loci plus those with emerging relevance This panel enables sequencing of Class I HLA A B and C Class II HLA DRB1 3 4 5 HLA DQA1 HLA DQB1 HLA DPA1 and HLA DPB1 This expands coverage beyond the classic loci with full gene coverage enabling discovery of new alleles without the need to design new primers Sample to Report Workflow with Optimized Data Analysis The comprehensive solution includes reagents and software optimized for HLA analysis A simplified workflow enables turnaround time of less than 48 hours and increased efficiency with less than 4 5 hours of hands on time On instrument software analyzes sequence data TruSight HLA Assign Software provides flexible options for post analysis reporting of results High Accuracy HLA Typing The expanded coverage of TruSight HLA v2 provides high resolution eliminating the need for follow up testing to obtain a confident typing result View HLA Loci How TruSight HLA v2 Works TruSight HLA v2 amplifies HLA loci with long range PCR After amplification Nextera tagmentation fragments the DNA and leaves behind a sequence tag An additional PCR then adds sequence adapters and indexing primers to generate sequencing ready DNA libraries Prepared libraries are then loaded directly onto a MiniSeq MiSeq or NextSeq System for sequencing Analysis Software Highlights The Assign 2 0 TruSight HLA analysis software assists with the assignment of a human leukocyte antigen HLA type The software is designed to analyze data from libraries prepared with Illumina TruSight HLA Sequencing Panels and then sequenced on an Illumina sequencer Use Assign 2 0 to import sequence data perform base calling edit sequences and compare a sample consensus sequence with the IMGT HLA database of alleles
    Catalog Number:
    20000215
    Price:
    None
    Category:
    Clinical Research Products
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    Structured Review

    Illumina Inc long range polymerase chain reaction trusight hla v2 sequencing panel
    TruSight HLA v2 Sequencing Panel
    The TruSight HLA v2 Sequencing Panel provides clinical researchers with a broad coverage ultra high resolution human leukocyte antigen HLA typing solution for simple rapid assessment of the HLA region in a single assay It features High resolution sequencing of 11 HLA loci Workflow with library preparation sequencing and data analysis reporting Accurate and high resolution HLA typing for unambiguous results Capture Full HLA Gene Sequences TruSight HLA v2 covers all commonly typed HLA loci plus those with emerging relevance This panel enables sequencing of Class I HLA A B and C Class II HLA DRB1 3 4 5 HLA DQA1 HLA DQB1 HLA DPA1 and HLA DPB1 This expands coverage beyond the classic loci with full gene coverage enabling discovery of new alleles without the need to design new primers Sample to Report Workflow with Optimized Data Analysis The comprehensive solution includes reagents and software optimized for HLA analysis A simplified workflow enables turnaround time of less than 48 hours and increased efficiency with less than 4 5 hours of hands on time On instrument software analyzes sequence data TruSight HLA Assign Software provides flexible options for post analysis reporting of results High Accuracy HLA Typing The expanded coverage of TruSight HLA v2 provides high resolution eliminating the need for follow up testing to obtain a confident typing result View HLA Loci How TruSight HLA v2 Works TruSight HLA v2 amplifies HLA loci with long range PCR After amplification Nextera tagmentation fragments the DNA and leaves behind a sequence tag An additional PCR then adds sequence adapters and indexing primers to generate sequencing ready DNA libraries Prepared libraries are then loaded directly onto a MiniSeq MiSeq or NextSeq System for sequencing Analysis Software Highlights The Assign 2 0 TruSight HLA analysis software assists with the assignment of a human leukocyte antigen HLA type The software is designed to analyze data from libraries prepared with Illumina TruSight HLA Sequencing Panels and then sequenced on an Illumina sequencer Use Assign 2 0 to import sequence data perform base calling edit sequences and compare a sample consensus sequence with the IMGT HLA database of alleles
    https://www.bioz.com/result/long range polymerase chain reaction trusight hla v2 sequencing panel/product/Illumina Inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    long range polymerase chain reaction trusight hla v2 sequencing panel - by Bioz Stars, 2020-09
    95/100 stars

    Related Products / Commonly Used Together

    hla locus genotyping

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    Related Articles

    Amplification:

    Article Title: Analysis of CD8+ T cell response during the 2013–2016 Ebola epidemic in West Africa
    Article Snippet: .. Long-range PCR amplification and library preparation was performed using the Illumina TruSight HLA v2 Sequencing Panel, according to the manufacturer’s protocols (Illumina). .. Next, 50 ng of genomic DNA was used for PCR amplification for each of the HLA class I loci.

    Article Title: Bead-linked transposomes enable a normalization-free workflow for NGS library preparation
    Article Snippet: .. TruSight HLA library pools were prepared using the TruSight HLA v2 Sequencing Panel (Illumina, cat. no. 20000215): 1 ng of each amplicon was individually tagmented, 10 μl of each tagmented amplicon was pooled before cleanup with SPBs; the manufacturer’s recommended protocol was followed except at the Normalize HLA PCR Amplicons step, where the two amplicon pools were manually quantitated to maintain consistency with the Nextera DNA Flex protocol. .. The fragment size profiles and yields of the libraries were determined, then libraries were sequenced on a NextSeq and coverage determined when downsampled to 25,000 reads per amplicon.

    Mutagenesis:

    Article Title: Immunopeptidomics of colorectal cancer organoids reveals a sparse HLA class I neoantigen landscape and no increase in neoantigens with interferon or MEK-inhibitor treatment
    Article Snippet: .. HLA typing and mutation calling 4-digit HLA typing was performed with the TruSight HLA v2 Panel on a MiniSeq (Illumina). ..

    Next-Generation Sequencing:

    Article Title: 17th IHIW component “Immunogenetics of Ageing” – New NGS data
    Article Snippet: .. The following loci: HLA-A, HLA-C, HLA-B, HLA-DRB1, HLA-DQA1, HLA-DQB1, HLA-DPA1 and HLA-DPB1 were typed by various NGS methods using commercially available kits: NGSgo and NGSengine software (GenDx, Utrecht, Netherlands); TruSight HLA v2 and Assign TruSight HLA software (Illumina, Inc., San Diego, CA); Holotype HLA and HLA Twin software (Omixon, Budapest, Hungary). .. Statistical analysis Python for Population Genomics (PyPop) software v.0.7.0 [ ] was used to calculate allele frequencies, 2-locus haplotype frequencies, standardized linkage disequilibrium measure D′, D′ chi-square, and the Ewens-Watterson’s homozygosity test of neutrality was applied to each locus in separate populations.

    Sequencing:

    Article Title: Bead-linked transposomes enable a normalization-free workflow for NGS library preparation
    Article Snippet: .. Amplicons were prepared according to the recommended protocol for the TruSight HLA v2 Sequencing Panel (Illumina, cat. no. 20007429). .. After amplification, PCR products were purified in bulk with a 0.7X concentration of SPBs before running 50 ng of each on a 1% agarose gel.

    Article Title: Mutational and putative neoantigen load predict clinical benefit of adoptive T cell therapy in melanoma
    Article Snippet: .. HLA typing and neoantigen prediction For HLA typing, sequencing libraries were prepared from normal DNA using Illumina TruSight HLA Sequencing Panel according to manufacturer’s instructions (Illumina) and sequenced on a MiSeq instrument (Illumina). .. In addition, HLA type was derived from WES data of normal (non-tumor) samples using bwakit ( https://github.com/lh3/bwa/tree/master/bwakit ) or Omixon Target HLA (Omixon).

    Article Title: Poster Presentations
    Article Snippet: .. Libraries were prepared with the Illumina Trusight HLA v2 Sequencing Panel and then sequenced on the MiSeq system. .. Results were analyzed with Assign 2.1 software.

    Article Title: Analysis of CD8+ T cell response during the 2013–2016 Ebola epidemic in West Africa
    Article Snippet: .. Long-range PCR amplification and library preparation was performed using the Illumina TruSight HLA v2 Sequencing Panel, according to the manufacturer’s protocols (Illumina). .. Next, 50 ng of genomic DNA was used for PCR amplification for each of the HLA class I loci.

    Article Title: Bead-linked transposomes enable a normalization-free workflow for NGS library preparation
    Article Snippet: .. TruSight HLA library pools were prepared using the TruSight HLA v2 Sequencing Panel (Illumina, cat. no. 20000215): 1 ng of each amplicon was individually tagmented, 10 μl of each tagmented amplicon was pooled before cleanup with SPBs; the manufacturer’s recommended protocol was followed except at the Normalize HLA PCR Amplicons step, where the two amplicon pools were manually quantitated to maintain consistency with the Nextera DNA Flex protocol. .. The fragment size profiles and yields of the libraries were determined, then libraries were sequenced on a NextSeq and coverage determined when downsampled to 25,000 reads per amplicon.

    Article Title: Recurrent genetic HLA loss in AML relapsed after matched unrelated allogeneic hematopoietic cell transplantation
    Article Snippet: .. 11 HLA locus genotyping was performed using the long-range polymerase chain reaction TruSight HLA v2 Sequencing Panel (Illumina; HLA-A, -B, -DRB1/3/4/5, -DQA1, -DQB1, -DPA1, and -DPB1). .. Paired-end 2 × 150 base-pair sequencing was performed (Illumina MiSeq), and data analysis was performed using TruSight HLA Assign 2.1 software.

    Polymerase Chain Reaction:

    Article Title: Analysis of CD8+ T cell response during the 2013–2016 Ebola epidemic in West Africa
    Article Snippet: .. Long-range PCR amplification and library preparation was performed using the Illumina TruSight HLA v2 Sequencing Panel, according to the manufacturer’s protocols (Illumina). .. Next, 50 ng of genomic DNA was used for PCR amplification for each of the HLA class I loci.

    Article Title: Bead-linked transposomes enable a normalization-free workflow for NGS library preparation
    Article Snippet: .. TruSight HLA library pools were prepared using the TruSight HLA v2 Sequencing Panel (Illumina, cat. no. 20000215): 1 ng of each amplicon was individually tagmented, 10 μl of each tagmented amplicon was pooled before cleanup with SPBs; the manufacturer’s recommended protocol was followed except at the Normalize HLA PCR Amplicons step, where the two amplicon pools were manually quantitated to maintain consistency with the Nextera DNA Flex protocol. .. The fragment size profiles and yields of the libraries were determined, then libraries were sequenced on a NextSeq and coverage determined when downsampled to 25,000 reads per amplicon.

    Article Title: Recurrent genetic HLA loss in AML relapsed after matched unrelated allogeneic hematopoietic cell transplantation
    Article Snippet: .. 11 HLA locus genotyping was performed using the long-range polymerase chain reaction TruSight HLA v2 Sequencing Panel (Illumina; HLA-A, -B, -DRB1/3/4/5, -DQA1, -DQB1, -DPA1, and -DPB1). .. Paired-end 2 × 150 base-pair sequencing was performed (Illumina MiSeq), and data analysis was performed using TruSight HLA Assign 2.1 software.

    Software:

    Article Title: 17th IHIW component “Immunogenetics of Ageing” – New NGS data
    Article Snippet: .. The following loci: HLA-A, HLA-C, HLA-B, HLA-DRB1, HLA-DQA1, HLA-DQB1, HLA-DPA1 and HLA-DPB1 were typed by various NGS methods using commercially available kits: NGSgo and NGSengine software (GenDx, Utrecht, Netherlands); TruSight HLA v2 and Assign TruSight HLA software (Illumina, Inc., San Diego, CA); Holotype HLA and HLA Twin software (Omixon, Budapest, Hungary). .. Statistical analysis Python for Population Genomics (PyPop) software v.0.7.0 [ ] was used to calculate allele frequencies, 2-locus haplotype frequencies, standardized linkage disequilibrium measure D′, D′ chi-square, and the Ewens-Watterson’s homozygosity test of neutrality was applied to each locus in separate populations.

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  • 95
    Illumina Inc long range polymerase chain reaction trusight hla v2 sequencing panel
    Long Range Polymerase Chain Reaction Trusight Hla V2 Sequencing Panel, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 95/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/long range polymerase chain reaction trusight hla v2 sequencing panel/product/Illumina Inc
    Average 95 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    long range polymerase chain reaction trusight hla v2 sequencing panel - by Bioz Stars, 2020-09
    95/100 stars
      Buy from Supplier

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