long range phusion pcr  (Thermo Fisher)


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    Structured Review

    Thermo Fisher long range phusion pcr
    Long Range Phusion Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/long range phusion pcr/product/Thermo Fisher
    Average 91 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    long range phusion pcr - by Bioz Stars, 2020-07
    91/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Novel “Superspreader” Bacteriophages Promote Horizontal Gene Transfer by Transformation
    Article Snippet: .. Long-range Phusion PCR (Thermo Scientific) was combined with the TapeStation platform (Agilent Technologies) to amplify and quantify intact plasmid pBAD24 extracted from phage lysates (see ). .. In contrast to the Southern blot assay and similar techniques, TapeStati on offers automated sample processing and accurate digital quantification.

    Article Title: Novel “Superspreader” Bacteriophages Promote Horizontal Gene Transfer by Transformation
    Article Snippet: .. We further explored this issue by combining long-range Phusion PCR (Thermo Fisher) with the TapeStation platform (Agilent Technologies) to amplify and quantify nearly the entire pBAD24 plasmid (4,480 of 4,542 bp or 98.6%), a product far too large to assess by ddPCR or by conventional quantitative PCR. .. In contrast to the ddPCR results but consistent with our initial transformation screening , SUSP2 lysates of MG/pBAD24 yielded approximately 10-fold greater band intensity (i.e., whole-plasmid copies) than did corresponding T4 lysates (see ).

    Article Title: Novel “Superspreader” Bacteriophages Promote Horizontal Gene Transfer by Transformation
    Article Snippet: .. DNA was extracted from SUSP2 and T4 lysates of ~108 E. coli MG1655 cells bearing ampicillin resistance plasmid pBAD24, amplified by long-range Phusion PCR, and quantified via TapeStation analysis. .. Relative band intensity represents the relative abundance of intact, whole plasmid pBAD24 (~4.5 kb) copies in SUSP2 lysates (lane C1) and T4 lysates (lane D1) of approximately 108 E. coli MG1655 cells bearing pBAD24.

    Real-time Polymerase Chain Reaction:

    Article Title: Novel “Superspreader” Bacteriophages Promote Horizontal Gene Transfer by Transformation
    Article Snippet: .. We further explored this issue by combining long-range Phusion PCR (Thermo Fisher) with the TapeStation platform (Agilent Technologies) to amplify and quantify nearly the entire pBAD24 plasmid (4,480 of 4,542 bp or 98.6%), a product far too large to assess by ddPCR or by conventional quantitative PCR. .. In contrast to the ddPCR results but consistent with our initial transformation screening , SUSP2 lysates of MG/pBAD24 yielded approximately 10-fold greater band intensity (i.e., whole-plasmid copies) than did corresponding T4 lysates (see ).

    Amplification:

    Article Title: Novel “Superspreader” Bacteriophages Promote Horizontal Gene Transfer by Transformation
    Article Snippet: .. DNA was extracted from SUSP2 and T4 lysates of ~108 E. coli MG1655 cells bearing ampicillin resistance plasmid pBAD24, amplified by long-range Phusion PCR, and quantified via TapeStation analysis. .. Relative band intensity represents the relative abundance of intact, whole plasmid pBAD24 (~4.5 kb) copies in SUSP2 lysates (lane C1) and T4 lysates (lane D1) of approximately 108 E. coli MG1655 cells bearing pBAD24.

    Plasmid Preparation:

    Article Title: Novel “Superspreader” Bacteriophages Promote Horizontal Gene Transfer by Transformation
    Article Snippet: .. Long-range Phusion PCR (Thermo Scientific) was combined with the TapeStation platform (Agilent Technologies) to amplify and quantify intact plasmid pBAD24 extracted from phage lysates (see ). .. In contrast to the Southern blot assay and similar techniques, TapeStati on offers automated sample processing and accurate digital quantification.

    Article Title: Novel “Superspreader” Bacteriophages Promote Horizontal Gene Transfer by Transformation
    Article Snippet: .. We further explored this issue by combining long-range Phusion PCR (Thermo Fisher) with the TapeStation platform (Agilent Technologies) to amplify and quantify nearly the entire pBAD24 plasmid (4,480 of 4,542 bp or 98.6%), a product far too large to assess by ddPCR or by conventional quantitative PCR. .. In contrast to the ddPCR results but consistent with our initial transformation screening , SUSP2 lysates of MG/pBAD24 yielded approximately 10-fold greater band intensity (i.e., whole-plasmid copies) than did corresponding T4 lysates (see ).

    Article Title: Novel “Superspreader” Bacteriophages Promote Horizontal Gene Transfer by Transformation
    Article Snippet: .. DNA was extracted from SUSP2 and T4 lysates of ~108 E. coli MG1655 cells bearing ampicillin resistance plasmid pBAD24, amplified by long-range Phusion PCR, and quantified via TapeStation analysis. .. Relative band intensity represents the relative abundance of intact, whole plasmid pBAD24 (~4.5 kb) copies in SUSP2 lysates (lane C1) and T4 lysates (lane D1) of approximately 108 E. coli MG1655 cells bearing pBAD24.

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    Thermo Fisher long range phusion pcr
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