Structured Review

Merck & Co long range pcr kit
Long Range Pcr Kit, supplied by Merck & Co, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/long range pcr kit/product/Merck & Co
Average 91 stars, based on 2 article reviews
Price from $9.99 to $1999.99
long range pcr kit - by Bioz Stars, 2020-07
91/100 stars

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Nested PCR:

Article Title: The PTAP sequence duplication in HIV-1 subtype C Gag p6 in drug-naive subjects of India and South Africa
Article Snippet: .. Amplification of Gag and the DNA sequencing The full-length gag was amplified from the cDNA using a nested-PCR strategy and a commercial long-range PCR kit (XT-20 PCR system, Merck Genie, India) on the I-Cycler (Bio-Rad, California, USA). .. Furthermore, from a select subset of the participants, Gag p6, LTR, Protease, RT, RNaseH, Integrase and Env regions were also amplified.

Article Title: PTAP motif duplication in the p6 Gag protein confers a replication advantage on HIV-1 subtype C
Article Snippet: .. The full-length gag or p6 gag sequence was amplified from the plasma RNA or genomic DNA samples (using 200–300 ng of DNA as a template) using a nested-PCR strategy and a commercial long-range PCR kit (XT-20 PCR system, Merck Genie, India) on the I-Cycler (Bio-Rad). .. Two different nested-PCR strategies were used to amplify full-length gag for the purpose of sequence determination by the conventional method and a third strategy that involves the amplification of a larger gag region along with the flanking sequences for the purpose of NGS.

Polymerase Chain Reaction:

Article Title: The PTAP sequence duplication in HIV-1 subtype C Gag p6 in drug-naive subjects of India and South Africa
Article Snippet: .. Amplification of Gag and the DNA sequencing The full-length gag was amplified from the cDNA using a nested-PCR strategy and a commercial long-range PCR kit (XT-20 PCR system, Merck Genie, India) on the I-Cycler (Bio-Rad, California, USA). .. Furthermore, from a select subset of the participants, Gag p6, LTR, Protease, RT, RNaseH, Integrase and Env regions were also amplified.

Article Title: PTAP motif duplication in the p6 Gag protein confers a replication advantage on HIV-1 subtype C
Article Snippet: .. The full-length gag or p6 gag sequence was amplified from the plasma RNA or genomic DNA samples (using 200–300 ng of DNA as a template) using a nested-PCR strategy and a commercial long-range PCR kit (XT-20 PCR system, Merck Genie, India) on the I-Cycler (Bio-Rad). .. Two different nested-PCR strategies were used to amplify full-length gag for the purpose of sequence determination by the conventional method and a third strategy that involves the amplification of a larger gag region along with the flanking sequences for the purpose of NGS.

Amplification:

Article Title: The PTAP sequence duplication in HIV-1 subtype C Gag p6 in drug-naive subjects of India and South Africa
Article Snippet: .. Amplification of Gag and the DNA sequencing The full-length gag was amplified from the cDNA using a nested-PCR strategy and a commercial long-range PCR kit (XT-20 PCR system, Merck Genie, India) on the I-Cycler (Bio-Rad, California, USA). .. Furthermore, from a select subset of the participants, Gag p6, LTR, Protease, RT, RNaseH, Integrase and Env regions were also amplified.

Article Title: PTAP motif duplication in the p6 Gag protein confers a replication advantage on HIV-1 subtype C
Article Snippet: .. The full-length gag or p6 gag sequence was amplified from the plasma RNA or genomic DNA samples (using 200–300 ng of DNA as a template) using a nested-PCR strategy and a commercial long-range PCR kit (XT-20 PCR system, Merck Genie, India) on the I-Cycler (Bio-Rad). .. Two different nested-PCR strategies were used to amplify full-length gag for the purpose of sequence determination by the conventional method and a third strategy that involves the amplification of a larger gag region along with the flanking sequences for the purpose of NGS.

Sequencing:

Article Title: PTAP motif duplication in the p6 Gag protein confers a replication advantage on HIV-1 subtype C
Article Snippet: .. The full-length gag or p6 gag sequence was amplified from the plasma RNA or genomic DNA samples (using 200–300 ng of DNA as a template) using a nested-PCR strategy and a commercial long-range PCR kit (XT-20 PCR system, Merck Genie, India) on the I-Cycler (Bio-Rad). .. Two different nested-PCR strategies were used to amplify full-length gag for the purpose of sequence determination by the conventional method and a third strategy that involves the amplification of a larger gag region along with the flanking sequences for the purpose of NGS.

DNA Sequencing:

Article Title: The PTAP sequence duplication in HIV-1 subtype C Gag p6 in drug-naive subjects of India and South Africa
Article Snippet: .. Amplification of Gag and the DNA sequencing The full-length gag was amplified from the cDNA using a nested-PCR strategy and a commercial long-range PCR kit (XT-20 PCR system, Merck Genie, India) on the I-Cycler (Bio-Rad, California, USA). .. Furthermore, from a select subset of the participants, Gag p6, LTR, Protease, RT, RNaseH, Integrase and Env regions were also amplified.

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    Merck & Co long range pcr kit
    Long Range Pcr Kit, supplied by Merck & Co, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/long range pcr kit/product/Merck & Co
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    long range pcr kit - by Bioz Stars, 2020-07
    91/100 stars
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