long amp taq 2x master mix  (New England Biolabs)


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    Name:
    Taq 2X Master Mix
    Description:
    Taq 2X Master Mix 500 rxns
    Catalog Number:
    m0270l
    Price:
    143
    Size:
    500 rxns
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    PCR related Kits
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    New England Biolabs long amp taq 2x master mix
    Taq 2X Master Mix
    Taq 2X Master Mix 500 rxns
    https://www.bioz.com/result/long amp taq 2x master mix/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    long amp taq 2x master mix - by Bioz Stars, 2020-04
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    Related Articles

    Clone Assay:

    Article Title: Absolute Quantification of the Host-To-Parasite DNA Ratio in Theileria parva-Infected Lymphocyte Cell Lines
    Article Snippet: The reaction mixture of 20 μL contained the following: 10μL of Taq 2X Master Mix (New England BioLabs), 1μL of the 10μM forward and reverse primers, 0.6 μL of 100% DMSO, 1μL of the DNA sample solution, and 6.4μL of PCR grade water. .. In each case, the PCR product was purified using the QIAquick Gel Extraction Kit (Qiagen) and cloned into a TOPO TA vector (Invitrogen).

    Article Title: ­­­Silencing of retrotransposons by SETDB1 inhibits the interferon response in acute myeloid leukemia­­
    Article Snippet: PCR thermocycling used 2× Taq Mastermix (NEB) at 98°C for 30 s, 40 cycles at 98°C for 10 s, 54°C for 30 s, and 72°C for 30 s, followed by 72°C for 5 min and 4°C. .. Resultant PCR products were gel-isolated, TOPO cloned into pCR2.1 (ThermoFisher), and sequenced.

    Amplification:

    Article Title: Prevalence of K13-propeller gene polymorphisms among Plasmodium falciparum parasites isolated from adult symptomatic patients in northern Uganda
    Article Snippet: Paragraph title: PCR amplification and sequencing of P. falciparum isolates ... First round PCR was run in a 25 μl reaction volume: briefly, 5.5 μl of nuclease free water (QIAGEN, Maryland, USA) was added to 12.5 μl of Taq 2X Master Mix (New England BioLabs, Massachusetts, USA).

    Article Title: Absolute Quantification of the Host-To-Parasite DNA Ratio in Theileria parva-Infected Lymphocyte Cell Lines
    Article Snippet: The expected size of the PCR product was 2,089 bp for ama1 and 1,221 bp for hprt1 and the fragments were amplified by end point PCR. .. The reaction mixture of 20 μL contained the following: 10μL of Taq 2X Master Mix (New England BioLabs), 1μL of the 10μM forward and reverse primers, 0.6 μL of 100% DMSO, 1μL of the DNA sample solution, and 6.4μL of PCR grade water.

    Article Title: ­­­Silencing of retrotransposons by SETDB1 inhibits the interferon response in acute myeloid leukemia­­
    Article Snippet: MDA5 sgRNAs 1 and 4 targeted loci were amplified with the same primers: forward, 5′-CGTCATTGTCAGGCACAGAG-3′, and reverse, 5′-ACAGTTCCTCCTCCATGCAC-3′. .. PCR thermocycling used 2× Taq Mastermix (NEB) at 98°C for 30 s, 40 cycles at 98°C for 10 s, 54°C for 30 s, and 72°C for 30 s, followed by 72°C for 5 min and 4°C.

    Article Title: Prevalence of molecular markers of sulfadoxine–pyrimethamine and artemisinin resistance in Plasmodium falciparum from Pakistan
    Article Snippet: .. Amplification and sequencing of pfk13 propeller domain The gene encoding PfK13 was amplified using Taq2X mastermix (New England BioLabs Inc., USA) in a 25 µL reaction using 12.5 µL of Taq2X mastermix, 1 µL of each 10 µM primer, 5.5 µL nuclease free water and 5 µL template DNA. .. The PCR thermal cycling conditions for the first round of nested PCR were 94 °C for 5 min followed by 40 cycles of 30 s at 94 °C, 90 s at 54 °C, 90 s at 68 °C and final extension at 68 °C for 10 min. 2 µL of primary PCR product was used as template in the secondary PCR using the same cycling conditions and primer set used previously [ ].

    Article Title: Genotypic and Phenotypic Characterization of Clostridium perfringens Isolates from Darmbrand Cases in Post-World War II Germany
    Article Snippet: Each PCR mixture contained 5 μl of template DNA, 25 μl of Taq 2× Master Mix (New England BioLabs), 1 μl of forward primer, 1 μl of reverse primer (1 μM final concentration), and 20 μl of distilled water. .. Overlapping PCRs were performed under the following amplification conditions: 35 cycles of 94°C for 30 s, 54°C for 30 s, and 68°C for 1 min, followed by a final single extension of 10 min at 68°C.

    Article Title: Association between MTHFR 677C > T Polymorphism and Vitamin B12 Deficiency: A Case-control Study
    Article Snippet: .. The amplification was performed in 25 μL final volume by using Taq 2X Master Mix (New England BioLabs, USA) according to the manufacturer’s instructions. .. Briefly, 3 μL of DNA were added to 12.5 μL of 2X master mix with a final concentration of primers 0.2 μmol/L and completed to 25 μL by adding dd.H2 O. PCR conditions were as follows: initial denaturation at 95 °C for 5 minutes, 35 cycles of denaturation at 95 °C for 1 minute, annealing (65 °C for the c.677C > T and 72 °C for the 1298A > C) for 30 seconds and extension at 72 °C for 1 minute, followed by 3 minutes of final extension at 72 °C.

    Article Title: Association between rs2431697 T allele on 5q33.3 and systemic lupus erythematosus: case-control study and meta-analysis
    Article Snippet: A 260-bp DNA fragment around rs2431697 was amplified with the primer pair: sense primer: 5′-AGAGGGGGTGAAAGAAGGAA-3′ and antisense primer: 5′-TTCTCAGTGCCAATGTGAGG-3′. .. The reaction mixtures contained 10 ng genomic DNA, 10 pmol of each primer, and double-diluted Taq 2× Master Mix (New England Biolabs, UK) in a total 20-μl volume.

    Article Title: An efficient method for recombineering GAL4 and QF drivers
    Article Snippet: .. For the colony PCR reactions, 5 pmoles each of primers 7, 8, 9 and 10 (primer locations are indicated on ) were used with Taq 2X master mix (New England Biolabs- Cat. # M0270L) in 20 µl reactions and amplified for 30 cycles. .. Templates were derived from bacterial colonies touched with a pipet tip that had been spotted onto a separate bacterial plate for subsequent recovery of positives.

    Construct:

    Article Title: Absolute Quantification of the Host-To-Parasite DNA Ratio in Theileria parva-Infected Lymphocyte Cell Lines
    Article Snippet: Construction of the plasmid standards for the quantitative PCR (qPCR) assay We constructed a plasmid standard for a parasite locus, the apical membrane antigen 1 (ama1 ) gene of T . parva from the Muguga isolate grown in animal BV115, and another for a bovine locus, the hypoxanthine phosphoribosyltransferase 1 (hprt1 ) gene amplified from gDNA from semen of a B . taurus primigenius animal, provided by the USDA, Agricultural Research Service, in Beltsville, Maryland. .. The reaction mixture of 20 μL contained the following: 10μL of Taq 2X Master Mix (New England BioLabs), 1μL of the 10μM forward and reverse primers, 0.6 μL of 100% DMSO, 1μL of the DNA sample solution, and 6.4μL of PCR grade water.

    Article Title: Evaluation of parameters affecting switchgrass tissue culture: toward a consolidated procedure for Agrobacterium-mediated transformation of switchgrass (Panicum virgatum)
    Article Snippet: The PCR analysis was performed using Taq 2x master mix (NEB, Ipswich, MA) with the appropriate primers, and the PCR reaction consisted of standard PCR with 35-cycles denaturing and annealing carried out at a temperature of 98 and 55 °C, respectively. .. The primer sets for GUSPlus (Gus-F and Gus-R) were derived from the literature [ ], while other primer sets were designed for specific genes in each construct, which are all listed in Additional file : Table S2.

    Real-time Polymerase Chain Reaction:

    Article Title: Absolute Quantification of the Host-To-Parasite DNA Ratio in Theileria parva-Infected Lymphocyte Cell Lines
    Article Snippet: Paragraph title: Construction of the plasmid standards for the quantitative PCR (qPCR) assay ... The reaction mixture of 20 μL contained the following: 10μL of Taq 2X Master Mix (New England BioLabs), 1μL of the 10μM forward and reverse primers, 0.6 μL of 100% DMSO, 1μL of the DNA sample solution, and 6.4μL of PCR grade water.

    Western Blot:

    Article Title: Association between rs2431697 T allele on 5q33.3 and systemic lupus erythematosus: case-control study and meta-analysis
    Article Snippet: Genotyping Genomic DNA was extracted and purified from peripheral blood with DNA Extractor WB Kit (Wako Pure Chemical Industries, Ltd. Japan) according to the product description. rs2431697 polymorphism was determined based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. .. The reaction mixtures contained 10 ng genomic DNA, 10 pmol of each primer, and double-diluted Taq 2× Master Mix (New England Biolabs, UK) in a total 20-μl volume.

    Derivative Assay:

    Article Title: An efficient method for recombineering GAL4 and QF drivers
    Article Snippet: For the colony PCR reactions, 5 pmoles each of primers 7, 8, 9 and 10 (primer locations are indicated on ) were used with Taq 2X master mix (New England Biolabs- Cat. # M0270L) in 20 µl reactions and amplified for 30 cycles. .. Templates were derived from bacterial colonies touched with a pipet tip that had been spotted onto a separate bacterial plate for subsequent recovery of positives.

    Article Title: Evaluation of parameters affecting switchgrass tissue culture: toward a consolidated procedure for Agrobacterium-mediated transformation of switchgrass (Panicum virgatum)
    Article Snippet: The PCR analysis was performed using Taq 2x master mix (NEB, Ipswich, MA) with the appropriate primers, and the PCR reaction consisted of standard PCR with 35-cycles denaturing and annealing carried out at a temperature of 98 and 55 °C, respectively. .. The primer sets for GUSPlus (Gus-F and Gus-R) were derived from the literature [ ], while other primer sets were designed for specific genes in each construct, which are all listed in Additional file : Table S2.

    Countercurrent Chromatography:

    Article Title: Retinal Neuroprotective Effects of Flibanserin, an FDA-Approved Dual Serotonin Receptor Agonist-Antagonist
    Article Snippet: .. PCR Genotyping PCR analysis was performed using Quick-load Taq (Taq 2x Master Mix, New England Biolabs, Ipswich, MA) and the following primer sequences (Integrated DNA Technologies, San Diego, CA): 5-HT1A promoter (forward): 5’-CAG TCT CTA GAT CCC CTC CCT-3’; 5-HT1A coding sequence (reverse): 5’-GGG CGT CCT CTT GTT CAC GTA-3’; and tTA insert (reverse): 5’-AAG GGC AAA AGT GAG TAT GGT-3’ . .. A 25 μL PCR reaction was performed in a Veriti thermal cycler (Applied Biosystems, Coster City, CA).

    Generated:

    Article Title: ­­­Silencing of retrotransposons by SETDB1 inhibits the interferon response in acute myeloid leukemia­­
    Article Snippet: Indel analysis To identify the mutations generated with SETDB1 -, RIG-I –, or MDA5 -specific sgRNAs, cells were transduced or nucleofected with sgRNAs or gRNAs, respectively. .. PCR thermocycling used 2× Taq Mastermix (NEB) at 98°C for 30 s, 40 cycles at 98°C for 10 s, 54°C for 30 s, and 72°C for 30 s, followed by 72°C for 5 min and 4°C.

    Polymerase Chain Reaction:

    Article Title: Prevalence of K13-propeller gene polymorphisms among Plasmodium falciparum parasites isolated from adult symptomatic patients in northern Uganda
    Article Snippet: .. First round PCR was run in a 25 μl reaction volume: briefly, 5.5 μl of nuclease free water (QIAGEN, Maryland, USA) was added to 12.5 μl of Taq 2X Master Mix (New England BioLabs, Massachusetts, USA). .. We used Taq 2X Master Mix with the final working concentration of the components at 1X.

    Article Title: Absolute Quantification of the Host-To-Parasite DNA Ratio in Theileria parva-Infected Lymphocyte Cell Lines
    Article Snippet: .. The reaction mixture of 20 μL contained the following: 10μL of Taq 2X Master Mix (New England BioLabs), 1μL of the 10μM forward and reverse primers, 0.6 μL of 100% DMSO, 1μL of the DNA sample solution, and 6.4μL of PCR grade water. .. PCR was performed using a BIO-RAD DNA Engine Thermal Cycler under the following conditions: an initial denaturation at 95°C for 15 minutes, followed by 35 cycles of 30 seconds at 95°C, 30 seconds at 60°C, and 2 minutes at 70°C.

    Article Title: ­­­Silencing of retrotransposons by SETDB1 inhibits the interferon response in acute myeloid leukemia­­
    Article Snippet: .. PCR thermocycling used 2× Taq Mastermix (NEB) at 98°C for 30 s, 40 cycles at 98°C for 10 s, 54°C for 30 s, and 72°C for 30 s, followed by 72°C for 5 min and 4°C. .. Resultant PCR products were gel-isolated, TOPO cloned into pCR2.1 (ThermoFisher), and sequenced.

    Article Title: Prevalence of molecular markers of sulfadoxine–pyrimethamine and artemisinin resistance in Plasmodium falciparum from Pakistan
    Article Snippet: Amplification and sequencing of pfk13 propeller domain The gene encoding PfK13 was amplified using Taq2X mastermix (New England BioLabs Inc., USA) in a 25 µL reaction using 12.5 µL of Taq2X mastermix, 1 µL of each 10 µM primer, 5.5 µL nuclease free water and 5 µL template DNA. .. The PCR thermal cycling conditions for the first round of nested PCR were 94 °C for 5 min followed by 40 cycles of 30 s at 94 °C, 90 s at 54 °C, 90 s at 68 °C and final extension at 68 °C for 10 min. 2 µL of primary PCR product was used as template in the secondary PCR using the same cycling conditions and primer set used previously [ ].

    Article Title: Genotypic and Phenotypic Characterization of Clostridium perfringens Isolates from Darmbrand Cases in Post-World War II Germany
    Article Snippet: .. Each PCR mixture contained 5 μl of template DNA, 25 μl of Taq 2× Master Mix (New England BioLabs), 1 μl of forward primer, 1 μl of reverse primer (1 μM final concentration), and 20 μl of distilled water. .. Long-range PCRs were performed with a Techne thermocycler using the following PCR conditions for the amplifications: 95°C for 2 min and 35 cycles of 94°C for 30 s, 54°C for 30 s, and 65°C for 3 min, followed by a final single extension of 10 min at 65°C.

    Article Title: Association between MTHFR 677C > T Polymorphism and Vitamin B12 Deficiency: A Case-control Study
    Article Snippet: Paragraph title: PCR amplification of the MTHFR target sequence ... The amplification was performed in 25 μL final volume by using Taq 2X Master Mix (New England BioLabs, USA) according to the manufacturer’s instructions.

    Article Title: Detection of OPCML methylation, a possible epigenetic marker, from free serum circulating DNA to improve the diagnosis of early-stage ovarian epithelial cancer
    Article Snippet: .. The PCR reactions included 0.2 µM forward and reverse primers, 0.1 µg template, 12.5 µl Taq 2X Master mix (New England BioLabs, Inc., Ipswich, MA, USA) and nuclease-free water. .. PCR mix without template DNA was used as a negative control.

    Article Title: Association between rs2431697 T allele on 5q33.3 and systemic lupus erythematosus: case-control study and meta-analysis
    Article Snippet: Genotyping Genomic DNA was extracted and purified from peripheral blood with DNA Extractor WB Kit (Wako Pure Chemical Industries, Ltd. Japan) according to the product description. rs2431697 polymorphism was determined based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. .. The reaction mixtures contained 10 ng genomic DNA, 10 pmol of each primer, and double-diluted Taq 2× Master Mix (New England Biolabs, UK) in a total 20-μl volume.

    Article Title: An efficient method for recombineering GAL4 and QF drivers
    Article Snippet: .. For the colony PCR reactions, 5 pmoles each of primers 7, 8, 9 and 10 (primer locations are indicated on ) were used with Taq 2X master mix (New England Biolabs- Cat. # M0270L) in 20 µl reactions and amplified for 30 cycles. .. Templates were derived from bacterial colonies touched with a pipet tip that had been spotted onto a separate bacterial plate for subsequent recovery of positives.

    Article Title: Prevalence of K13-propeller gene polymorphisms among Plasmodium falciparum parasites isolated from adult symptomatic patients in northern Uganda
    Article Snippet: Paragraph title: PCR amplification and sequencing of P. falciparum isolates ... This was run in a 50 μl reaction volume; briefly, 19 μl of nuclease free water (QIAGEN, Maryland, USA) was added to 25 μl of Taq 2X Master Mix (New England BioLabs, Massachusetts, USA).

    Article Title: Retinal Neuroprotective Effects of Flibanserin, an FDA-Approved Dual Serotonin Receptor Agonist-Antagonist
    Article Snippet: .. PCR Genotyping PCR analysis was performed using Quick-load Taq (Taq 2x Master Mix, New England Biolabs, Ipswich, MA) and the following primer sequences (Integrated DNA Technologies, San Diego, CA): 5-HT1A promoter (forward): 5’-CAG TCT CTA GAT CCC CTC CCT-3’; 5-HT1A coding sequence (reverse): 5’-GGG CGT CCT CTT GTT CAC GTA-3’; and tTA insert (reverse): 5’-AAG GGC AAA AGT GAG TAT GGT-3’ . .. A 25 μL PCR reaction was performed in a Veriti thermal cycler (Applied Biosystems, Coster City, CA).

    Article Title: Evaluation of parameters affecting switchgrass tissue culture: toward a consolidated procedure for Agrobacterium-mediated transformation of switchgrass (Panicum virgatum)
    Article Snippet: .. The PCR analysis was performed using Taq 2x master mix (NEB, Ipswich, MA) with the appropriate primers, and the PCR reaction consisted of standard PCR with 35-cycles denaturing and annealing carried out at a temperature of 98 and 55 °C, respectively. .. The primer sets for GUSPlus (Gus-F and Gus-R) were derived from the literature [ ], while other primer sets were designed for specific genes in each construct, which are all listed in Additional file : Table S2.

    DNA Extraction:

    Article Title: Evaluation of parameters affecting switchgrass tissue culture: toward a consolidated procedure for Agrobacterium-mediated transformation of switchgrass (Panicum virgatum)
    Article Snippet: Paragraph title: Genomic DNA extraction and PCR analysis ... The PCR analysis was performed using Taq 2x master mix (NEB, Ipswich, MA) with the appropriate primers, and the PCR reaction consisted of standard PCR with 35-cycles denaturing and annealing carried out at a temperature of 98 and 55 °C, respectively.

    Methylation:

    Article Title: Detection of OPCML methylation, a possible epigenetic marker, from free serum circulating DNA to improve the diagnosis of early-stage ovarian epithelial cancer
    Article Snippet: Paragraph title: Methylation of DNA was detected using nested PCR ... The PCR reactions included 0.2 µM forward and reverse primers, 0.1 µg template, 12.5 µl Taq 2X Master mix (New England BioLabs, Inc., Ipswich, MA, USA) and nuclease-free water.

    Isolation:

    Article Title: ­­­Silencing of retrotransposons by SETDB1 inhibits the interferon response in acute myeloid leukemia­­
    Article Snippet: After 5–7 d of treatment, genomic DNA was isolated with a DNeasy blood and tissue kit. .. PCR thermocycling used 2× Taq Mastermix (NEB) at 98°C for 30 s, 40 cycles at 98°C for 10 s, 54°C for 30 s, and 72°C for 30 s, followed by 72°C for 5 min and 4°C.

    Purification:

    Article Title: Absolute Quantification of the Host-To-Parasite DNA Ratio in Theileria parva-Infected Lymphocyte Cell Lines
    Article Snippet: The reaction mixture of 20 μL contained the following: 10μL of Taq 2X Master Mix (New England BioLabs), 1μL of the 10μM forward and reverse primers, 0.6 μL of 100% DMSO, 1μL of the DNA sample solution, and 6.4μL of PCR grade water. .. In each case, the PCR product was purified using the QIAquick Gel Extraction Kit (Qiagen) and cloned into a TOPO TA vector (Invitrogen).

    Article Title: Prevalence of molecular markers of sulfadoxine–pyrimethamine and artemisinin resistance in Plasmodium falciparum from Pakistan
    Article Snippet: Amplification and sequencing of pfk13 propeller domain The gene encoding PfK13 was amplified using Taq2X mastermix (New England BioLabs Inc., USA) in a 25 µL reaction using 12.5 µL of Taq2X mastermix, 1 µL of each 10 µM primer, 5.5 µL nuclease free water and 5 µL template DNA. .. Amplification and sequencing of pfk13 propeller domain The gene encoding PfK13 was amplified using Taq2X mastermix (New England BioLabs Inc., USA) in a 25 µL reaction using 12.5 µL of Taq2X mastermix, 1 µL of each 10 µM primer, 5.5 µL nuclease free water and 5 µL template DNA.

    Article Title: Genotypic and Phenotypic Characterization of Clostridium perfringens Isolates from Darmbrand Cases in Post-World War II Germany
    Article Snippet: These analyses used the primers listed in and template DNA purified using the MasterPure Gram-positive DNA purification kit. .. Each PCR mixture contained 5 μl of template DNA, 25 μl of Taq 2× Master Mix (New England BioLabs), 1 μl of forward primer, 1 μl of reverse primer (1 μM final concentration), and 20 μl of distilled water.

    Article Title: Association between rs2431697 T allele on 5q33.3 and systemic lupus erythematosus: case-control study and meta-analysis
    Article Snippet: Genotyping Genomic DNA was extracted and purified from peripheral blood with DNA Extractor WB Kit (Wako Pure Chemical Industries, Ltd. Japan) according to the product description. rs2431697 polymorphism was determined based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. .. The reaction mixtures contained 10 ng genomic DNA, 10 pmol of each primer, and double-diluted Taq 2× Master Mix (New England Biolabs, UK) in a total 20-μl volume.

    Sequencing:

    Article Title: Prevalence of K13-propeller gene polymorphisms among Plasmodium falciparum parasites isolated from adult symptomatic patients in northern Uganda
    Article Snippet: Paragraph title: PCR amplification and sequencing of P. falciparum isolates ... First round PCR was run in a 25 μl reaction volume: briefly, 5.5 μl of nuclease free water (QIAGEN, Maryland, USA) was added to 12.5 μl of Taq 2X Master Mix (New England BioLabs, Massachusetts, USA).

    Article Title: Prevalence of molecular markers of sulfadoxine–pyrimethamine and artemisinin resistance in Plasmodium falciparum from Pakistan
    Article Snippet: .. Amplification and sequencing of pfk13 propeller domain The gene encoding PfK13 was amplified using Taq2X mastermix (New England BioLabs Inc., USA) in a 25 µL reaction using 12.5 µL of Taq2X mastermix, 1 µL of each 10 µM primer, 5.5 µL nuclease free water and 5 µL template DNA. .. The PCR thermal cycling conditions for the first round of nested PCR were 94 °C for 5 min followed by 40 cycles of 30 s at 94 °C, 90 s at 54 °C, 90 s at 68 °C and final extension at 68 °C for 10 min. 2 µL of primary PCR product was used as template in the secondary PCR using the same cycling conditions and primer set used previously [ ].

    Article Title: Association between MTHFR 677C > T Polymorphism and Vitamin B12 Deficiency: A Case-control Study
    Article Snippet: Paragraph title: PCR amplification of the MTHFR target sequence ... The amplification was performed in 25 μL final volume by using Taq 2X Master Mix (New England BioLabs, USA) according to the manufacturer’s instructions.

    Article Title: Retinal Neuroprotective Effects of Flibanserin, an FDA-Approved Dual Serotonin Receptor Agonist-Antagonist
    Article Snippet: .. PCR Genotyping PCR analysis was performed using Quick-load Taq (Taq 2x Master Mix, New England Biolabs, Ipswich, MA) and the following primer sequences (Integrated DNA Technologies, San Diego, CA): 5-HT1A promoter (forward): 5’-CAG TCT CTA GAT CCC CTC CCT-3’; 5-HT1A coding sequence (reverse): 5’-GGG CGT CCT CTT GTT CAC GTA-3’; and tTA insert (reverse): 5’-AAG GGC AAA AGT GAG TAT GGT-3’ . .. A 25 μL PCR reaction was performed in a Veriti thermal cycler (Applied Biosystems, Coster City, CA).

    Staining:

    Article Title: Genotypic and Phenotypic Characterization of Clostridium perfringens Isolates from Darmbrand Cases in Post-World War II Germany
    Article Snippet: Each PCR mixture contained 5 μl of template DNA, 25 μl of Taq 2× Master Mix (New England BioLabs), 1 μl of forward primer, 1 μl of reverse primer (1 μM final concentration), and 20 μl of distilled water. .. PCR products were separated on a 1.5% agarose gel and visualized by ethidium bromide staining.

    Article Title: Prevalence of K13-propeller gene polymorphisms among Plasmodium falciparum parasites isolated from adult symptomatic patients in northern Uganda
    Article Snippet: This was run in a 50 μl reaction volume; briefly, 19 μl of nuclease free water (QIAGEN, Maryland, USA) was added to 25 μl of Taq 2X Master Mix (New England BioLabs, Massachusetts, USA). .. All PCR products were analyzed using 1 % (w/v) Agarose gel (Fisher Scientific, USA), stained with 0.05 μg/mL of Ethidium Bromide (Sigma Aldrich, USA).

    Nested PCR:

    Article Title: Prevalence of K13-propeller gene polymorphisms among Plasmodium falciparum parasites isolated from adult symptomatic patients in northern Uganda
    Article Snippet: PCR amplification and sequencing of P. falciparum isolates For amplification of the K13 gene, we used nested PCR to amplify 849 bp fragment extending from nucleotides 1329-to-2178 (codons 445-to-727) within the entire K13-propeller domain. .. First round PCR was run in a 25 μl reaction volume: briefly, 5.5 μl of nuclease free water (QIAGEN, Maryland, USA) was added to 12.5 μl of Taq 2X Master Mix (New England BioLabs, Massachusetts, USA).

    Article Title: Prevalence of molecular markers of sulfadoxine–pyrimethamine and artemisinin resistance in Plasmodium falciparum from Pakistan
    Article Snippet: Amplification and sequencing of pfk13 propeller domain The gene encoding PfK13 was amplified using Taq2X mastermix (New England BioLabs Inc., USA) in a 25 µL reaction using 12.5 µL of Taq2X mastermix, 1 µL of each 10 µM primer, 5.5 µL nuclease free water and 5 µL template DNA. .. The PCR thermal cycling conditions for the first round of nested PCR were 94 °C for 5 min followed by 40 cycles of 30 s at 94 °C, 90 s at 54 °C, 90 s at 68 °C and final extension at 68 °C for 10 min. 2 µL of primary PCR product was used as template in the secondary PCR using the same cycling conditions and primer set used previously [ ].

    Article Title: Detection of OPCML methylation, a possible epigenetic marker, from free serum circulating DNA to improve the diagnosis of early-stage ovarian epithelial cancer
    Article Snippet: Paragraph title: Methylation of DNA was detected using nested PCR ... The PCR reactions included 0.2 µM forward and reverse primers, 0.1 µg template, 12.5 µl Taq 2X Master mix (New England BioLabs, Inc., Ipswich, MA, USA) and nuclease-free water.

    Plasmid Preparation:

    Article Title: Absolute Quantification of the Host-To-Parasite DNA Ratio in Theileria parva-Infected Lymphocyte Cell Lines
    Article Snippet: Paragraph title: Construction of the plasmid standards for the quantitative PCR (qPCR) assay ... The reaction mixture of 20 μL contained the following: 10μL of Taq 2X Master Mix (New England BioLabs), 1μL of the 10μM forward and reverse primers, 0.6 μL of 100% DMSO, 1μL of the DNA sample solution, and 6.4μL of PCR grade water.

    Negative Control:

    Article Title: Detection of OPCML methylation, a possible epigenetic marker, from free serum circulating DNA to improve the diagnosis of early-stage ovarian epithelial cancer
    Article Snippet: The PCR reactions included 0.2 µM forward and reverse primers, 0.1 µg template, 12.5 µl Taq 2X Master mix (New England BioLabs, Inc., Ipswich, MA, USA) and nuclease-free water. .. PCR mix without template DNA was used as a negative control.

    Selection:

    Article Title: Evaluation of parameters affecting switchgrass tissue culture: toward a consolidated procedure for Agrobacterium-mediated transformation of switchgrass (Panicum virgatum)
    Article Snippet: The PCR analysis was performed using Taq 2x master mix (NEB, Ipswich, MA) with the appropriate primers, and the PCR reaction consisted of standard PCR with 35-cycles denaturing and annealing carried out at a temperature of 98 and 55 °C, respectively. .. Selection efficiency was counted as the number of genomic PCR positive switchgrass transformants divided by the regenerated hygromycin B resistant switchgrass transformants.

    Agarose Gel Electrophoresis:

    Article Title: Genotypic and Phenotypic Characterization of Clostridium perfringens Isolates from Darmbrand Cases in Post-World War II Germany
    Article Snippet: Each PCR mixture contained 5 μl of template DNA, 25 μl of Taq 2× Master Mix (New England BioLabs), 1 μl of forward primer, 1 μl of reverse primer (1 μM final concentration), and 20 μl of distilled water. .. PCR products were separated on a 1.5% agarose gel and visualized by ethidium bromide staining.

    Article Title: Association between rs2431697 T allele on 5q33.3 and systemic lupus erythematosus: case-control study and meta-analysis
    Article Snippet: The reaction mixtures contained 10 ng genomic DNA, 10 pmol of each primer, and double-diluted Taq 2× Master Mix (New England Biolabs, UK) in a total 20-μl volume. .. The reaction conditions were as follows: denaturation at 94 °C for 5 min, followed by 35ccycles of denaturation at 94 °C for 30 s, annealing for 1 min at 57 °C and extension at 72 °C for 45 s, and a final extension at 72 °C for 5 min. PCR products were subsequently digested by Taq I (New England Biolabs, UK) at 65 °C for 2 h and separated on a 3 % agarose gel.

    Article Title: Prevalence of K13-propeller gene polymorphisms among Plasmodium falciparum parasites isolated from adult symptomatic patients in northern Uganda
    Article Snippet: This was run in a 50 μl reaction volume; briefly, 19 μl of nuclease free water (QIAGEN, Maryland, USA) was added to 25 μl of Taq 2X Master Mix (New England BioLabs, Massachusetts, USA). .. All PCR products were analyzed using 1 % (w/v) Agarose gel (Fisher Scientific, USA), stained with 0.05 μg/mL of Ethidium Bromide (Sigma Aldrich, USA).

    Transgenic Assay:

    Article Title: Evaluation of parameters affecting switchgrass tissue culture: toward a consolidated procedure for Agrobacterium-mediated transformation of switchgrass (Panicum virgatum)
    Article Snippet: Genomic DNA extraction and PCR analysis Approximately 50–100 mg of young leaves of WT or putative transgenic switchgrass plantlets (2-week-old) were collected into 2 ml safe-lock Eppendorf tubes and stored in liquid nitrogen for genomic DNA extraction. .. The PCR analysis was performed using Taq 2x master mix (NEB, Ipswich, MA) with the appropriate primers, and the PCR reaction consisted of standard PCR with 35-cycles denaturing and annealing carried out at a temperature of 98 and 55 °C, respectively.

    Concentration Assay:

    Article Title: Prevalence of K13-propeller gene polymorphisms among Plasmodium falciparum parasites isolated from adult symptomatic patients in northern Uganda
    Article Snippet: First round PCR was run in a 25 μl reaction volume: briefly, 5.5 μl of nuclease free water (QIAGEN, Maryland, USA) was added to 12.5 μl of Taq 2X Master Mix (New England BioLabs, Massachusetts, USA). .. We used Taq 2X Master Mix with the final working concentration of the components at 1X.

    Article Title: Genotypic and Phenotypic Characterization of Clostridium perfringens Isolates from Darmbrand Cases in Post-World War II Germany
    Article Snippet: .. Each PCR mixture contained 5 μl of template DNA, 25 μl of Taq 2× Master Mix (New England BioLabs), 1 μl of forward primer, 1 μl of reverse primer (1 μM final concentration), and 20 μl of distilled water. .. Long-range PCRs were performed with a Techne thermocycler using the following PCR conditions for the amplifications: 95°C for 2 min and 35 cycles of 94°C for 30 s, 54°C for 30 s, and 65°C for 3 min, followed by a final single extension of 10 min at 65°C.

    Article Title: Association between MTHFR 677C > T Polymorphism and Vitamin B12 Deficiency: A Case-control Study
    Article Snippet: The amplification was performed in 25 μL final volume by using Taq 2X Master Mix (New England BioLabs, USA) according to the manufacturer’s instructions. .. Briefly, 3 μL of DNA were added to 12.5 μL of 2X master mix with a final concentration of primers 0.2 μmol/L and completed to 25 μL by adding dd.H2 O. PCR conditions were as follows: initial denaturation at 95 °C for 5 minutes, 35 cycles of denaturation at 95 °C for 1 minute, annealing (65 °C for the c.677C > T and 72 °C for the 1298A > C) for 30 seconds and extension at 72 °C for 1 minute, followed by 3 minutes of final extension at 72 °C.

    Article Title: Prevalence of K13-propeller gene polymorphisms among Plasmodium falciparum parasites isolated from adult symptomatic patients in northern Uganda
    Article Snippet: The concentration of the components at 1X were: 10 mM Tris-HCl, 50 mM KCl, 1.5 mM MgCl2 , 0.2 mM dNTPs, 25 units/ml Taq DNA Polymerase, 5 % Glycerol, 0.08 % IGEPAL® CA-630, 0.05 % Tween®, pH 8.6@25 °C). .. This was run in a 50 μl reaction volume; briefly, 19 μl of nuclease free water (QIAGEN, Maryland, USA) was added to 25 μl of Taq 2X Master Mix (New England BioLabs, Massachusetts, USA).

    DNA Purification:

    Article Title: Genotypic and Phenotypic Characterization of Clostridium perfringens Isolates from Darmbrand Cases in Post-World War II Germany
    Article Snippet: These analyses used the primers listed in and template DNA purified using the MasterPure Gram-positive DNA purification kit. .. Each PCR mixture contained 5 μl of template DNA, 25 μl of Taq 2× Master Mix (New England BioLabs), 1 μl of forward primer, 1 μl of reverse primer (1 μM final concentration), and 20 μl of distilled water.

    Gel Extraction:

    Article Title: Absolute Quantification of the Host-To-Parasite DNA Ratio in Theileria parva-Infected Lymphocyte Cell Lines
    Article Snippet: The reaction mixture of 20 μL contained the following: 10μL of Taq 2X Master Mix (New England BioLabs), 1μL of the 10μM forward and reverse primers, 0.6 μL of 100% DMSO, 1μL of the DNA sample solution, and 6.4μL of PCR grade water. .. In each case, the PCR product was purified using the QIAquick Gel Extraction Kit (Qiagen) and cloned into a TOPO TA vector (Invitrogen).

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    New England Biolabs long amp tag 2x master mix kit
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    New England Biolabs long amp taq 2x master mix
    Long Amp Taq 2x Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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