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TaKaRa log phase xl1 blue e coli cells
Log Phase Xl1 Blue E Coli Cells, supplied by TaKaRa, used in various techniques. Bioz Stars score: 87/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/log phase xl1 blue e coli cells/product/TaKaRa
Average 87 stars, based on 3 article reviews
Price from $9.99 to $1999.99
log phase xl1 blue e coli cells - by Bioz Stars, 2020-04
87/100 stars

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Clone Assay:

Article Title: An Insight into the Sialome of the Black Fly, Simulium vittatum
Article Snippet: The packaged library was plated by infecting log-phase XL1- Blue Escherichia coli cells (Clontech). .. The percentage of recombinant clones was determined by blue-white selection screening on LB/MgSO4 plates containing X-gal/IPTG.

Article Title: An Insight into the Sialotranscriptome of Triatoma matogrossensis, a Kissing Bug Associated with Fogo Selvagem in South America
Article Snippet: The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech). .. The percentage of recombinant clones was determined by performing a blue-white selection screening on LB/MgSO4 plates containing X-gal/isopropyl β-d-1-thiogalactopyranoside (IPTG).

Article Title: An insight into the sialome of Simulium guianense (DIPTERA:SIMulIIDAE), the main vector of River Blindness Disease in Brazil
Article Snippet: The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech). .. The percentage of recombinant clones was determined by blue-white selection screening on LB/MgSO4 plates containing X-gal/IPTG.

Article Title: An insight into the salivary transcriptome and proteome of the soft tick and vector of epizootic bovine abortion, Ornithodoros coriaceus
Article Snippet: The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech). .. The percentage of recombinant clones was determined by performing a blue-white selection screening on LB/MgSO4 plates containing X-gal/IPTG.

Article Title: An Insight Into the Sialotranscriptome of Triatoma rubida (Hemiptera: Heteroptera)
Article Snippet: The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech). .. The percentage of recombinant clones was determined by blue–white selection screening on LB/MgSO4 plates containing X-gal/IPTG.

Article Title: An Insight into the Sialotranscriptome of the Cat Flea, Ctenocephalides felis
Article Snippet: The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech). .. The percentage of recombinant clones was determined by blue-white selection screening on LB/MgSO4 plates containing X-gal/IPTG.

Article Title: An insight into the sialome of the oriental rat flea, Xenopsylla cheopis (Rots)
Article Snippet: The packaged library was plated by infecting log phase XL1 Blue E. coli cells (Clontech, Palo Alto, CA). .. The percentage of recombinant clones was determined by performing a blue-white selection screening on LB/MgSO4 plates containing X-gal/IPTG.

Article Title: The sialotranscriptome of Antricola delacruzi female ticks is compatible with non-hematophagous behavior and an alternative source of food
Article Snippet: The packaged library was plated by infecting log phase XL1-Blue E. coli cells (Clontech, Palo Alto, CA). .. The percentage of recombinant clones was determined by performing a blue–white selection screening on LB/MgSO4 plates containing X-gal/IPTG.

Article Title: An Insight into the Sialotranscriptome of Simulium nigrimanum, a Black Fly Associated with Fogo Selvagem in South America
Article Snippet: The packaged library was plated by infecting log-phase XL1- Blue Escherichia coli cells (Clontech). .. The percentage of recombinant clones was determined by blue-white selection screening on LB/MgSO4 plates containing X-gal/IPTG.

Article Title: An insight into the sialotranscriptome of the brown dog tick, Rhipicephalus sanguineus
Article Snippet: The cDNA synthesis procedure resulted in the creation of Sfi I A and B restriction enzyme sites at the ends of the PCR products that are used for cloning into the phage vector. .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: Insight into the Salivary Transcriptome and Proteome of Dipetalogaster maxima
Article Snippet: The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech). .. The percentage of recombinant clones was determined by blue-white selection screening on LB/MgSO4 plates containing X-gal/IPTG.

Article Title: An insight into the sialome of the bed bug, Cimex lectularius
Article Snippet: The packaged library was plated by infecting log-phase XL1- Blue Escherichia coli cells (Clontech). .. The percentage of recombinant clones was determined by blue-white selection screening on LB/MgSO4 plates containing X-gal/IPTG.

Selection:

Article Title: An Insight into the Sialome of the Black Fly, Simulium vittatum
Article Snippet: The packaged library was plated by infecting log-phase XL1- Blue Escherichia coli cells (Clontech). .. The percentage of recombinant clones was determined by blue-white selection screening on LB/MgSO4 plates containing X-gal/IPTG.

Article Title: An Insight into the Sialotranscriptome of Triatoma matogrossensis, a Kissing Bug Associated with Fogo Selvagem in South America
Article Snippet: The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech). .. The percentage of recombinant clones was determined by performing a blue-white selection screening on LB/MgSO4 plates containing X-gal/isopropyl β-d-1-thiogalactopyranoside (IPTG).

Article Title: An insight into the sialome of Simulium guianense (DIPTERA:SIMulIIDAE), the main vector of River Blindness Disease in Brazil
Article Snippet: The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech). .. The percentage of recombinant clones was determined by blue-white selection screening on LB/MgSO4 plates containing X-gal/IPTG.

Article Title: An insight into the salivary transcriptome and proteome of the soft tick and vector of epizootic bovine abortion, Ornithodoros coriaceus
Article Snippet: The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech). .. The percentage of recombinant clones was determined by performing a blue-white selection screening on LB/MgSO4 plates containing X-gal/IPTG.

Article Title: An Insight Into the Sialotranscriptome of Triatoma rubida (Hemiptera: Heteroptera)
Article Snippet: The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech). .. The percentage of recombinant clones was determined by blue–white selection screening on LB/MgSO4 plates containing X-gal/IPTG.

Article Title: An insight into the sialotranscriptome of the seed-feeding bug, Oncopeltus fasciatus
Article Snippet: The packaged library was plated by infecting log-phase XL1-Blue E. coli cells (Clontech). .. The percentage of re-clones was determined by performing a blue-white selection screening on LB/MgSO4 plates containing X-gal/IPTG.

Article Title: An Insight into the Sialotranscriptome of the Cat Flea, Ctenocephalides felis
Article Snippet: The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech). .. The percentage of recombinant clones was determined by blue-white selection screening on LB/MgSO4 plates containing X-gal/IPTG.

Article Title: An insight into the sialome of the oriental rat flea, Xenopsylla cheopis (Rots)
Article Snippet: The packaged library was plated by infecting log phase XL1 Blue E. coli cells (Clontech, Palo Alto, CA). .. The percentage of recombinant clones was determined by performing a blue-white selection screening on LB/MgSO4 plates containing X-gal/IPTG.

Article Title: The sialotranscriptome of Antricola delacruzi female ticks is compatible with non-hematophagous behavior and an alternative source of food
Article Snippet: The packaged library was plated by infecting log phase XL1-Blue E. coli cells (Clontech, Palo Alto, CA). .. The percentage of recombinant clones was determined by performing a blue–white selection screening on LB/MgSO4 plates containing X-gal/IPTG.

Article Title: An Insight into the Sialotranscriptome of Simulium nigrimanum, a Black Fly Associated with Fogo Selvagem in South America
Article Snippet: The packaged library was plated by infecting log-phase XL1- Blue Escherichia coli cells (Clontech). .. The percentage of recombinant clones was determined by blue-white selection screening on LB/MgSO4 plates containing X-gal/IPTG.

Article Title: An insight into the sialotranscriptome of the brown dog tick, Rhipicephalus sanguineus
Article Snippet: The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech). .. The percentage of recombinant clones was determined by performing a blue-white selection screening on LB/MgSO4 plates containing X-gal/IPTG.

Article Title: Insight into the Salivary Transcriptome and Proteome of Dipetalogaster maxima
Article Snippet: The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech). .. The percentage of recombinant clones was determined by blue-white selection screening on LB/MgSO4 plates containing X-gal/IPTG.

Article Title: An insight into the sialome of the bed bug, Cimex lectularius
Article Snippet: The packaged library was plated by infecting log-phase XL1- Blue Escherichia coli cells (Clontech). .. The percentage of recombinant clones was determined by blue-white selection screening on LB/MgSO4 plates containing X-gal/IPTG.

Agarose Gel Electrophoresis:

Article Title: An Insight into the Sialome of the Black Fly, Simulium vittatum
Article Snippet: The profile of the fractions was checked on a 1.1% agarose gel, and fractions containing cDNAs of more than 400 bp were pooled and concentrated using a Microcon YM-100. .. The packaged library was plated by infecting log-phase XL1- Blue Escherichia coli cells (Clontech).

Article Title: An insight into the salivary transcriptome and proteome of the soft tick and vector of epizootic bovine abortion, Ornithodoros coriaceus
Article Snippet: The profile of the fractions was checked on a 1.1% agarose gel, and fractions containing cDNAs of more than 400 bp were pooled and concentrated using a Microcon YM-100. .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: An insight into the sialome of the oriental rat flea, Xenopsylla cheopis (Rots)
Article Snippet: The profile of the fractions was checked on a 1.1% agarose gel and fractions containing cDNAs of more than 400 bp were pooled and concentrated using a Microcon YM100. .. The packaged library was plated by infecting log phase XL1 Blue E. coli cells (Clontech, Palo Alto, CA).

Article Title: The sialotranscriptome of Antricola delacruzi female ticks is compatible with non-hematophagous behavior and an alternative source of food
Article Snippet: The profile of the fractions was checked on a 1.1% agarose gel and fractions containing cDNAs of more than 400 bp were pooled and concentrated using a Microcon YM-100. .. The packaged library was plated by infecting log phase XL1-Blue E. coli cells (Clontech, Palo Alto, CA).

Article Title: An Insight into the Sialotranscriptome of Simulium nigrimanum, a Black Fly Associated with Fogo Selvagem in South America
Article Snippet: The cleaned, double-stranded cDNA was then digested with SfiI at 50°C for 2 hours, followed by size fractionation on a ChromaSpin-400 column (Clontech) into small (S), medium (M), and large (L) transcripts based on their electrophoresis profile on a 1.1% agarose gel. .. The packaged library was plated by infecting log-phase XL1- Blue Escherichia coli cells (Clontech).

Article Title: An insight into the sialome of the bed bug, Cimex lectularius
Article Snippet: The profile of the fractions was checked on a 1.1% agarose gel, and fractions containing cDNAs of more than 400 bp were pooled and concentrated using a Microcon YM-100. .. The packaged library was plated by infecting log-phase XL1- Blue Escherichia coli cells (Clontech).

Ligation:

Article Title: An Insight into the Sialome of the Black Fly, Simulium vittatum
Article Snippet: The cDNA mixture was ligated into the λ TriplEx2 vector (Clontech), and the resulting ligation mixture was packaged using the GigaPack® III Plus packaging extract (Stratagene, La Jolla, CA) according to the manufacturer’s instructions. .. The packaged library was plated by infecting log-phase XL1- Blue Escherichia coli cells (Clontech).

Article Title: An Insight into the Sialotranscriptome of Triatoma matogrossensis, a Kissing Bug Associated with Fogo Selvagem in South America
Article Snippet: The concentrated cDNA was then ligated into a λ TriplEx2 vector (Clontech), and the resulting ligation mixture was packaged using GigaPack Gold III Plus packaging extract (Stratagene, La Jolla, CA) according to the manufacturer's instructions. .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: An insight into the sialome of Simulium guianense (DIPTERA:SIMulIIDAE), the main vector of River Blindness Disease in Brazil
Article Snippet: The concentrated cDNA mixture was ligated into the λ TriplEx2 vector, and the resulting ligation mixture was packaged using the GigaPack® III Plus packaging extract (Stratagene, La Jolla, CA) according to the manufacturer's instructions. .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: An insight into the salivary transcriptome and proteome of the soft tick and vector of epizootic bovine abortion, Ornithodoros coriaceus
Article Snippet: The cDNA mixture was ligated into the λ TriplEx2 vector (Clontech), and the resulting ligation mixture was packaged using the GigaPack® III Plus packaging extract (Stratagene, La Jolla, CA) according to the manufacturer’s instructions. .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: An Insight Into the Sialotranscriptome of Triatoma rubida (Hemiptera: Heteroptera)
Article Snippet: Concentrated cDNA was then ligated into a λ TriplEx2 vector (Clontech), and the resulting ligation mixture was packaged using GigaPack Gold III Plus packaging extract (Agilent Technologies, Santa Clara, CA) according to the manufacturer’s instructions. .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: An insight into the sialotranscriptome of the seed-feeding bug, Oncopeltus fasciatus
Article Snippet: The cDNAs were then ligated into a λ TriplEx2 vector (Clontech), and the resulting ligation mixture was packaged using GigaPack® III Plus packaging extract (Stratagene) according to the manufacturer's instructions. .. The packaged library was plated by infecting log-phase XL1-Blue E. coli cells (Clontech).

Article Title: Differential Expression Profiles in the Midgut of Triatoma infestans Infected with Trypanosoma cruzi
Article Snippet: The cDNA was precipitated and ligated into a λTriplEx2 vector (Clontech), and the ligation was packaged using GigaPack Gold III Plus packaging extract (Stratagene) according to the manufacturer's guidelines. .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech) for cDNA library amplification and titering unamplified and amplified libraries.

Article Title: An Insight into the Sialotranscriptome of the Cat Flea, Ctenocephalides felis
Article Snippet: The concentrated cDNA mixture was ligated into the λ TriplEx2 vector, and the resulting ligation mixture was packaged using the GigaPack® III Plus packaging extract (Stratagene, La Jolla, CA, USA) according to the manufacturer's instructions. .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: An insight into the sialome of the oriental rat flea, Xenopsylla cheopis (Rots)
Article Snippet: The cDNA mixture was ligated into the λ TriplEx2 vector (Clontech, Palo Alto, CA) and the resulting ligation mixture was packaged using the GigaPack® III Plus packaging extract (Stratagene, La Jolla, CA) according to the manufacturer's instructions. .. The packaged library was plated by infecting log phase XL1 Blue E. coli cells (Clontech, Palo Alto, CA).

Article Title: The sialotranscriptome of Antricola delacruzi female ticks is compatible with non-hematophagous behavior and an alternative source of food
Article Snippet: The cDNA mixture was ligated into the TriplEx2 vector (Clontech, Palo Alto, CA) and the resulting ligation mixture was packaged using the GigaPacks III Plus packaging extract (Stratagene, La Jolla, CA) according to the manufacturer’s instructions. .. The packaged library was plated by infecting log phase XL1-Blue E. coli cells (Clontech, Palo Alto, CA).

Article Title: An Insight into the Sialotranscriptome of Simulium nigrimanum, a Black Fly Associated with Fogo Selvagem in South America
Article Snippet: The concentrated cDNA mixture was ligated into the λ TriplEx2 vector (Clontech), and the resulting ligation mixture was packaged using the GigaPack III Plus packaging extract (Stratagene, La Jolla, CA) according to the manufacturer's instructions. .. The packaged library was plated by infecting log-phase XL1- Blue Escherichia coli cells (Clontech).

Article Title: An insight into the sialotranscriptome of the brown dog tick, Rhipicephalus sanguineus
Article Snippet: The cDNA mixture was ligated into the λ TriplEx2 vector (Clontech) and the resulting ligation mixture was packaged using the GigaPack® III Plus packaging extract (Stratagene, La Jolla, CA) according to the manufacturer's instructions. .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: Insight into the Salivary Transcriptome and Proteome of Dipetalogaster maxima
Article Snippet: Concentrated cDNA was then ligated into a λ TriplEx2 vector (Clontech), and the resulting ligation mixture was packaged using GigaPack® Gold III Plus packaging extract (Stratagene, La Jolla, CA, USA) according to the manufacturer’s instructions. .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: An insight into the sialome of the bed bug, Cimex lectularius
Article Snippet: The cDNA mixture was ligated into the λ TriplEx2 vector (Clontech), and the resulting ligation mixture was packaged using the GigaPack® III Plus packaging extract (Stratagene, La Jolla, CA) according to the manufacturer's instructions. .. The packaged library was plated by infecting log-phase XL1- Blue Escherichia coli cells (Clontech).

Isolation:

Article Title: An Insight into the Sialome of the Black Fly, Simulium vittatum
Article Snippet: Paragraph title: Salivary Gland Isolation and Library Construction ... The packaged library was plated by infecting log-phase XL1- Blue Escherichia coli cells (Clontech).

Article Title: An insight into the salivary transcriptome and proteome of the soft tick and vector of epizootic bovine abortion, Ornithodoros coriaceus
Article Snippet: Paragraph title: 2.3. Salivary gland isolation and library construction ... The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: An annotated catalogue of salivary gland transcripts in the adult female mosquito, Ædes ægypti*
Article Snippet: Paragraph title: SG isolation ... The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: An insight into the sialome of the oriental rat flea, Xenopsylla cheopis (Rots)
Article Snippet: Paragraph title: Salivary gland isolation and library construction ... The packaged library was plated by infecting log phase XL1 Blue E. coli cells (Clontech, Palo Alto, CA).

Article Title: The sialotranscriptome of Antricola delacruzi female ticks is compatible with non-hematophagous behavior and an alternative source of food
Article Snippet: Paragraph title: 2.2. Salivary gland isolation and library construction ... The packaged library was plated by infecting log phase XL1-Blue E. coli cells (Clontech, Palo Alto, CA).

Article Title: An insight into the sialome of the bed bug, Cimex lectularius
Article Snippet: Paragraph title: Salivary Gland Isolation and Library Construction ... The packaged library was plated by infecting log-phase XL1- Blue Escherichia coli cells (Clontech).

Synthesized:

Article Title: An Insight into the Sialome of the Black Fly, Simulium vittatum
Article Snippet: PCR conditions were as follows: 95°C for 20 sec; 24 cycles of 95°C for 5 sec., 68°C for 6 min. A small portion of the cDNA obtained by PCR was analyzed on a 1.1% agarose gel to check quality and range of cDNA synthesized. .. The packaged library was plated by infecting log-phase XL1- Blue Escherichia coli cells (Clontech).

Article Title: An insight into the sialome of Simulium guianense (DIPTERA:SIMulIIDAE), the main vector of River Blindness Disease in Brazil
Article Snippet: PCR conditions were as follows: 95°C for 1 min; 26 cycles of 95°C for 15 sec, 68°C for 6 min. A small portion of the cDNA obtained by PCR was analyzed on an E-Gel® 1.2% with SYBR Safe (Invitrogen) to check quality and range of cDNA synthesized. .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: An insight into the sialotranscriptome of the seed-feeding bug, Oncopeltus fasciatus
Article Snippet: The PCR conditions were: 95° C for 20 s; 24 cycles of 95° C for 5 s; 68° C for 6 min. A small portion of the cDNA was analyzed on a 1.1% agarose/EtBr (0.1 μg/ml) gel to check for the quality and the range of the cDNA synthesized. .. The packaged library was plated by infecting log-phase XL1-Blue E. coli cells (Clontech).

Article Title: An Insight into the Sialotranscriptome of the Cat Flea, Ctenocephalides felis
Article Snippet: PCR conditions were as follows: 95°C for 1 min; 22 cycles of 95°C for 15 sec, 68°C for 6 min. A small portion of the cDNA obtained by PCR was analyzed on an E-Gel® 1.2% agarose/EtBr (Invitrogen) to check quality and range of cDNA synthesized. .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: The sialotranscriptome of Antricola delacruzi female ticks is compatible with non-hematophagous behavior and an alternative source of food
Article Snippet: The PCR conditions were: 95 °C for 20 s; 24 cycles of 95 °C for 5 s, 68 °C for 6 min. A small portion of the cDNA obtained by PCR was analyzed on a 1.1% agarose gel to check for the quality and range of cDNA synthesized. .. The packaged library was plated by infecting log phase XL1-Blue E. coli cells (Clontech, Palo Alto, CA).

Article Title: An Insight into the Sialotranscriptome of Simulium nigrimanum, a Black Fly Associated with Fogo Selvagem in South America
Article Snippet: The PCR conditions were as follows: 95°C for 1 min; 24 cycles of 95°C for 10 sec, 68°C for 6 min. A small portion of the cDNA obtained by PCR was analyzed on a 1.1% agarose gel to check quality and range of cDNA synthesized. .. The packaged library was plated by infecting log-phase XL1- Blue Escherichia coli cells (Clontech).

Article Title: An insight into the sialome of the bed bug, Cimex lectularius
Article Snippet: PCR conditions were as follows: 95°C for 20 sec; 24 cycles of 95°C for 5 sec., 68°C for 6 min. A small portion of the cDNA obtained by PCR was analyzed on a 1.1% agarose gel to check quality and range of cDNA synthesized. .. The packaged library was plated by infecting log-phase XL1- Blue Escherichia coli cells (Clontech).

Polymerase Chain Reaction:

Article Title: An Insight into the Sialome of the Black Fly, Simulium vittatum
Article Snippet: PCR conditions were as follows: 95°C for 20 sec; 24 cycles of 95°C for 5 sec., 68°C for 6 min. A small portion of the cDNA obtained by PCR was analyzed on a 1.1% agarose gel to check quality and range of cDNA synthesized. .. The packaged library was plated by infecting log-phase XL1- Blue Escherichia coli cells (Clontech).

Article Title: An insight into the sialome of Simulium guianense (DIPTERA:SIMulIIDAE), the main vector of River Blindness Disease in Brazil
Article Snippet: PCR conditions were as follows: 95°C for 1 min; 26 cycles of 95°C for 15 sec, 68°C for 6 min. A small portion of the cDNA obtained by PCR was analyzed on an E-Gel® 1.2% with SYBR Safe (Invitrogen) to check quality and range of cDNA synthesized. .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: An insight into the salivary transcriptome and proteome of the soft tick and vector of epizootic bovine abortion, Ornithodoros coriaceus
Article Snippet: The PCR conditions were: 95°C for 20 sec; 24 cycles of 95°C for 5 sec, 68°C for 6 min. A small portion of the cDNA obtained by PCR was analysed on a 1.1% agarose gel to check for the quality and range of cDNA synthesised. .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: An insight into the sialotranscriptome of the seed-feeding bug, Oncopeltus fasciatus
Article Snippet: The PCR conditions were: 95° C for 20 s; 24 cycles of 95° C for 5 s; 68° C for 6 min. A small portion of the cDNA was analyzed on a 1.1% agarose/EtBr (0.1 μg/ml) gel to check for the quality and the range of the cDNA synthesized. .. The packaged library was plated by infecting log-phase XL1-Blue E. coli cells (Clontech).

Article Title: Differential Expression Profiles in the Midgut of Triatoma infestans Infected with Trypanosoma cruzi
Article Snippet: The PCR conditions were 95°C for 1 min, 19 cycles of 95°C for 15 s and 68°C for 6 min. A 5-µL sample was analyzed on a 1.1% agarose/EtBr (0.1 mg/mL) gel to check the quality and abundance of the cDNA. .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech) for cDNA library amplification and titering unamplified and amplified libraries.

Article Title: An Insight into the Sialotranscriptome of the Cat Flea, Ctenocephalides felis
Article Snippet: PCR conditions were as follows: 95°C for 1 min; 22 cycles of 95°C for 15 sec, 68°C for 6 min. A small portion of the cDNA obtained by PCR was analyzed on an E-Gel® 1.2% agarose/EtBr (Invitrogen) to check quality and range of cDNA synthesized. .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: An insight into the sialome of the oriental rat flea, Xenopsylla cheopis (Rots)
Article Snippet: The PCR conditions were: 95°C for 20 sec; 24 cycles of 95°C for 5 sec., 68°C for 6 min. A small portion of the cDNA obtained by PCR was analysed on a 1.1% agarose gel to check for the quality and range of cDNA synthesised. .. The packaged library was plated by infecting log phase XL1 Blue E. coli cells (Clontech, Palo Alto, CA).

Article Title: The sialotranscriptome of Antricola delacruzi female ticks is compatible with non-hematophagous behavior and an alternative source of food
Article Snippet: The PCR conditions were: 95 °C for 20 s; 24 cycles of 95 °C for 5 s, 68 °C for 6 min. A small portion of the cDNA obtained by PCR was analyzed on a 1.1% agarose gel to check for the quality and range of cDNA synthesized. .. The packaged library was plated by infecting log phase XL1-Blue E. coli cells (Clontech, Palo Alto, CA).

Article Title: An Insight into the Sialotranscriptome of Simulium nigrimanum, a Black Fly Associated with Fogo Selvagem in South America
Article Snippet: The PCR conditions were as follows: 95°C for 1 min; 24 cycles of 95°C for 10 sec, 68°C for 6 min. A small portion of the cDNA obtained by PCR was analyzed on a 1.1% agarose gel to check quality and range of cDNA synthesized. .. The packaged library was plated by infecting log-phase XL1- Blue Escherichia coli cells (Clontech).

Article Title: An insight into the sialotranscriptome of the brown dog tick, Rhipicephalus sanguineus
Article Snippet: A small portion of the cDNA obtained by PCR was analyzed on a 1.1% agarose gel with ethidium bromide (1.5 μg/mL). .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: An insight into the sialome of the bed bug, Cimex lectularius
Article Snippet: PCR conditions were as follows: 95°C for 20 sec; 24 cycles of 95°C for 5 sec., 68°C for 6 min. A small portion of the cDNA obtained by PCR was analyzed on a 1.1% agarose gel to check quality and range of cDNA synthesized. .. The packaged library was plated by infecting log-phase XL1- Blue Escherichia coli cells (Clontech).

Size-exclusion Chromatography:

Article Title: An Insight into the Sialome of the Black Fly, Simulium vittatum
Article Snippet: PCR conditions were as follows: 95°C for 20 sec; 24 cycles of 95°C for 5 sec., 68°C for 6 min. A small portion of the cDNA obtained by PCR was analyzed on a 1.1% agarose gel to check quality and range of cDNA synthesized. .. The packaged library was plated by infecting log-phase XL1- Blue Escherichia coli cells (Clontech).

Article Title: An insight into the sialome of Simulium guianense (DIPTERA:SIMulIIDAE), the main vector of River Blindness Disease in Brazil
Article Snippet: PCR conditions were as follows: 95°C for 1 min; 26 cycles of 95°C for 15 sec, 68°C for 6 min. A small portion of the cDNA obtained by PCR was analyzed on an E-Gel® 1.2% with SYBR Safe (Invitrogen) to check quality and range of cDNA synthesized. .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: An insight into the salivary transcriptome and proteome of the soft tick and vector of epizootic bovine abortion, Ornithodoros coriaceus
Article Snippet: The PCR conditions were: 95°C for 20 sec; 24 cycles of 95°C for 5 sec, 68°C for 6 min. A small portion of the cDNA obtained by PCR was analysed on a 1.1% agarose gel to check for the quality and range of cDNA synthesised. .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: An Insight into the Sialotranscriptome of the Cat Flea, Ctenocephalides felis
Article Snippet: PCR conditions were as follows: 95°C for 1 min; 22 cycles of 95°C for 15 sec, 68°C for 6 min. A small portion of the cDNA obtained by PCR was analyzed on an E-Gel® 1.2% agarose/EtBr (Invitrogen) to check quality and range of cDNA synthesized. .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: An insight into the sialome of the oriental rat flea, Xenopsylla cheopis (Rots)
Article Snippet: The PCR conditions were: 95°C for 20 sec; 24 cycles of 95°C for 5 sec., 68°C for 6 min. A small portion of the cDNA obtained by PCR was analysed on a 1.1% agarose gel to check for the quality and range of cDNA synthesised. .. The packaged library was plated by infecting log phase XL1 Blue E. coli cells (Clontech, Palo Alto, CA).

Article Title: An Insight into the Sialotranscriptome of Simulium nigrimanum, a Black Fly Associated with Fogo Selvagem in South America
Article Snippet: The PCR conditions were as follows: 95°C for 1 min; 24 cycles of 95°C for 10 sec, 68°C for 6 min. A small portion of the cDNA obtained by PCR was analyzed on a 1.1% agarose gel to check quality and range of cDNA synthesized. .. The packaged library was plated by infecting log-phase XL1- Blue Escherichia coli cells (Clontech).

Article Title: An insight into the sialome of the bed bug, Cimex lectularius
Article Snippet: PCR conditions were as follows: 95°C for 20 sec; 24 cycles of 95°C for 5 sec., 68°C for 6 min. A small portion of the cDNA obtained by PCR was analyzed on a 1.1% agarose gel to check quality and range of cDNA synthesized. .. The packaged library was plated by infecting log-phase XL1- Blue Escherichia coli cells (Clontech).

Purification:

Article Title: An Insight Into the Sialotranscriptome of Triatoma rubida (Hemiptera: Heteroptera)
Article Snippet: The purified double-stranded cDNA was then digested with Sfi I restriction enzyme at 50°C for 2 h followed by size fractionation on a Chroma-Spin–400 drip column (Clontech). .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Electrophoresis:

Article Title: An insight into the sialome of Simulium guianense (DIPTERA:SIMulIIDAE), the main vector of River Blindness Disease in Brazil
Article Snippet: The cleaned, double-stranded cDNA was then digested with SfiI at 50°C for 2 h, followed by size fractionation on a ChromaSpin-400 column (Clontech) into small (S), medium (M), and large (L) transcripts based on their electrophoresis profile on an E-Gel® 1.2% with SYBR Safe. .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: An Insight into the Sialotranscriptome of the Cat Flea, Ctenocephalides felis
Article Snippet: The cleaned double-stranded cDNA was then digested with SfiI restriction enzyme at 50°C for 2 h, followed by size fractionation on a ChromaSpin–400 drip column (Clontech) into small (S), medium (M), and large (L) transcripts based on their electrophoresis profile on an E-Gel® 1.2%agarose/EtBr. .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: An Insight into the Sialotranscriptome of Simulium nigrimanum, a Black Fly Associated with Fogo Selvagem in South America
Article Snippet: The cleaned, double-stranded cDNA was then digested with SfiI at 50°C for 2 hours, followed by size fractionation on a ChromaSpin-400 column (Clontech) into small (S), medium (M), and large (L) transcripts based on their electrophoresis profile on a 1.1% agarose gel. .. The packaged library was plated by infecting log-phase XL1- Blue Escherichia coli cells (Clontech).

Sequencing:

Article Title: An Insight into the Sialome of the Black Fly, Simulium vittatum
Article Snippet: The packaged library was plated by infecting log-phase XL1- Blue Escherichia coli cells (Clontech). .. Recombinants were also determined by PCR, using vector primers (5/ λ TriplEx2 sequencing primer and 3/ λ TriplEx2 sequencing) flanking the inserted cDNA, with subsequent visualization of the products on a 1.1% agarose/EtBr gel.

Article Title: An insight into the salivary transcriptome and proteome of the soft tick and vector of epizootic bovine abortion, Ornithodoros coriaceus
Article Snippet: The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech). .. Recombinants were also determined by PCR, using vector primers (5′ λ TriplEx2 and 3′ λ TriplEx2 sequencing primers) flanking the inserted cDNA and visualising the products on a 1.1% agarose/EtBr gel.

Article Title: An insight into the sialotranscriptome of the seed-feeding bug, Oncopeltus fasciatus
Article Snippet: The packaged library was plated by infecting log-phase XL1-Blue E. coli cells (Clontech). .. Recombinants were also determined by PCR, using vector primers (5′ λ TriplEx2 and 3′ λ TriplEx2 sequencing primers) flanking the inserted cDNA and visualizing the products on a 1.1% agarose/EtBr gel.

Article Title: An annotated catalogue of salivary gland transcripts in the adult female mosquito, Ædes ægypti*
Article Snippet: The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech). .. Recombinants were also determined by PCR, using vector primers (5' λ TriplEx2 and 3' λ TriplEx2 sequencing primers) flanking the inserted cDNA and visualizing the products on a 1.1% agarose/EtBr gel.

Article Title: An insight into the sialome of the oriental rat flea, Xenopsylla cheopis (Rots)
Article Snippet: The packaged library was plated by infecting log phase XL1 Blue E. coli cells (Clontech, Palo Alto, CA). .. Recombinants were also determined by PCR, using vector primers (5' λ TriplEx2 Sequencing Primer and 3' λ TriplEx2 Sequencing) flanking the inserted cDNA and visualising the products on a 1.1% agarose/EtBr gel.

Article Title: The sialotranscriptome of Antricola delacruzi female ticks is compatible with non-hematophagous behavior and an alternative source of food
Article Snippet: The packaged library was plated by infecting log phase XL1-Blue E. coli cells (Clontech, Palo Alto, CA). .. Recombinants were also determined by PCR, using vector primers (50 μl TriplEx2 Sequencing Primer and 30 μl TriplEx2 Sequencing) flanking the inserted cDNA and visualizing the products on a 1.1% agarose/EtBr gel.

Article Title: An insight into the sialotranscriptome of the brown dog tick, Rhipicephalus sanguineus
Article Snippet: Paragraph title: cDNA library construction and sequencing ... The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: An insight into the sialome of the bed bug, Cimex lectularius
Article Snippet: The packaged library was plated by infecting log-phase XL1- Blue Escherichia coli cells (Clontech). .. Recombinants were also determined by PCR, using vector primers (5′ λ TriplEx2 sequencing primer and 3′ λ TriplEx2 sequencing) flanking the inserted cDNA, with subsequent visualization of the products on a 1.1% agarose/EtBr gel.

Amplification:

Article Title: Differential Expression Profiles in the Midgut of Triatoma infestans Infected with Trypanosoma cruzi
Article Snippet: .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech) for cDNA library amplification and titering unamplified and amplified libraries. .. Sequencing of T. infestans cDNA libraries The E. coli BM 25.8 strain was used to inoculate 2 mL of LB broth medium at 31°C with shaking at 180 rpm until the OD600 reached 1.3.

Fractionation:

Article Title: An Insight into the Sialome of the Black Fly, Simulium vittatum
Article Snippet: The cleaned, double-stranded cDNA was then digested with SfiI at 50°C for 2 hours, followed by size fractionation on a ChromaSpin– 400 column (Clontech). .. The packaged library was plated by infecting log-phase XL1- Blue Escherichia coli cells (Clontech).

Article Title: An Insight into the Sialotranscriptome of Triatoma matogrossensis, a Kissing Bug Associated with Fogo Selvagem in South America
Article Snippet: The clean double-stranded cDNA was then digested with SfiI restriction enzyme at 50°C for 2 hr followed by size fractionation on a ChromaSpin-400 drip column (Clontech). .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: An insight into the sialome of Simulium guianense (DIPTERA:SIMulIIDAE), the main vector of River Blindness Disease in Brazil
Article Snippet: The cleaned, double-stranded cDNA was then digested with SfiI at 50°C for 2 h, followed by size fractionation on a ChromaSpin-400 column (Clontech) into small (S), medium (M), and large (L) transcripts based on their electrophoresis profile on an E-Gel® 1.2% with SYBR Safe. .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: An insight into the salivary transcriptome and proteome of the soft tick and vector of epizootic bovine abortion, Ornithodoros coriaceus
Article Snippet: The cleaned, double-stranded cDNA was then digested with SfiI at 50°C for 2 h, followed by size fractionation on a ChromaSpin–400 column (Clontech). .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: An Insight Into the Sialotranscriptome of Triatoma rubida (Hemiptera: Heteroptera)
Article Snippet: The purified double-stranded cDNA was then digested with Sfi I restriction enzyme at 50°C for 2 h followed by size fractionation on a Chroma-Spin–400 drip column (Clontech). .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: An insight into the sialotranscriptome of the seed-feeding bug, Oncopeltus fasciatus
Article Snippet: The clean, double-stranded cDNA was then digested with SfiI restriction enzyme at 50° C for 2 h, followed by size fractionation on a ChromaSpin–400 drip column (Clontech). .. The packaged library was plated by infecting log-phase XL1-Blue E. coli cells (Clontech).

Article Title: An Insight into the Sialotranscriptome of the Cat Flea, Ctenocephalides felis
Article Snippet: The cleaned double-stranded cDNA was then digested with SfiI restriction enzyme at 50°C for 2 h, followed by size fractionation on a ChromaSpin–400 drip column (Clontech) into small (S), medium (M), and large (L) transcripts based on their electrophoresis profile on an E-Gel® 1.2%agarose/EtBr. .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: An insight into the sialome of the oriental rat flea, Xenopsylla cheopis (Rots)
Article Snippet: The cleaned, doublestranded cDNA was then digested with SfiI at 50°C for 2 hrs, followed by size fractionation on a ChromaSpin-400 column (Clontech, Palo Alto, CA). .. The packaged library was plated by infecting log phase XL1 Blue E. coli cells (Clontech, Palo Alto, CA).

Article Title: The sialotranscriptome of Antricola delacruzi female ticks is compatible with non-hematophagous behavior and an alternative source of food
Article Snippet: The cleaned, double-stranded cDNA was then digested with SfiI at 50 °C for 2 h, followed by size fractionation on a ChromaSpin-400 column (Clontech, Palo Alto, CA). .. The packaged library was plated by infecting log phase XL1-Blue E. coli cells (Clontech, Palo Alto, CA).

Article Title: An Insight into the Sialotranscriptome of Simulium nigrimanum, a Black Fly Associated with Fogo Selvagem in South America
Article Snippet: The cleaned, double-stranded cDNA was then digested with SfiI at 50°C for 2 hours, followed by size fractionation on a ChromaSpin-400 column (Clontech) into small (S), medium (M), and large (L) transcripts based on their electrophoresis profile on a 1.1% agarose gel. .. The packaged library was plated by infecting log-phase XL1- Blue Escherichia coli cells (Clontech).

Article Title: An insight into the sialotranscriptome of the brown dog tick, Rhipicephalus sanguineus
Article Snippet: The cleaned, double-stranded cDNA was then digested with Sfi I at 50°C for 2 h, followed by size fractionation on a ChromaSpin-1000 column (Clontech). .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: Insight into the Salivary Transcriptome and Proteome of Dipetalogaster maxima
Article Snippet: The clean double-stranded cDNA was then digested with Sfi I restriction enzyme at 50°C for 2 h followed by size fractionation on a ChromaSpin–400 drip column (Clontech). .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: An insight into the sialome of the bed bug, Cimex lectularius
Article Snippet: The cleaned, double-stranded cDNA was then digested with SfiI at 50°C for 2 hours, followed by size fractionation on a ChromaSpin– 400 column (Clontech). .. The packaged library was plated by infecting log-phase XL1- Blue Escherichia coli cells (Clontech).

cDNA Library Assay:

Article Title: An Insight into the Sialotranscriptome of Triatoma matogrossensis, a Kissing Bug Associated with Fogo Selvagem in South America
Article Snippet: Paragraph title: Triatoma matogrossensis and SG cDNA library construction. ... The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: An Insight Into the Sialotranscriptome of Triatoma rubida (Hemiptera: Heteroptera)
Article Snippet: Paragraph title: T. rubida and Salivary Glands cDNA Library Construction ... The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: Differential Expression Profiles in the Midgut of Triatoma infestans Infected with Trypanosoma cruzi
Article Snippet: .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech) for cDNA library amplification and titering unamplified and amplified libraries. .. Sequencing of T. infestans cDNA libraries The E. coli BM 25.8 strain was used to inoculate 2 mL of LB broth medium at 31°C with shaking at 180 rpm until the OD600 reached 1.3.

Article Title: An insight into the sialotranscriptome of the brown dog tick, Rhipicephalus sanguineus
Article Snippet: Paragraph title: cDNA library construction and sequencing ... The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: Insight into the Salivary Transcriptome and Proteome of Dipetalogaster maxima
Article Snippet: Paragraph title: Dm and SG cDNA Library Construction ... The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Recombinant:

Article Title: An Insight into the Sialome of the Black Fly, Simulium vittatum
Article Snippet: The packaged library was plated by infecting log-phase XL1- Blue Escherichia coli cells (Clontech). .. The percentage of recombinant clones was determined by blue-white selection screening on LB/MgSO4 plates containing X-gal/IPTG.

Article Title: An Insight into the Sialotranscriptome of Triatoma matogrossensis, a Kissing Bug Associated with Fogo Selvagem in South America
Article Snippet: The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech). .. The percentage of recombinant clones was determined by performing a blue-white selection screening on LB/MgSO4 plates containing X-gal/isopropyl β-d-1-thiogalactopyranoside (IPTG).

Article Title: An insight into the sialome of Simulium guianense (DIPTERA:SIMulIIDAE), the main vector of River Blindness Disease in Brazil
Article Snippet: The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech). .. The percentage of recombinant clones was determined by blue-white selection screening on LB/MgSO4 plates containing X-gal/IPTG.

Article Title: An insight into the salivary transcriptome and proteome of the soft tick and vector of epizootic bovine abortion, Ornithodoros coriaceus
Article Snippet: The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech). .. The percentage of recombinant clones was determined by performing a blue-white selection screening on LB/MgSO4 plates containing X-gal/IPTG.

Article Title: An Insight Into the Sialotranscriptome of Triatoma rubida (Hemiptera: Heteroptera)
Article Snippet: The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech). .. The percentage of recombinant clones was determined by blue–white selection screening on LB/MgSO4 plates containing X-gal/IPTG.

Article Title: An Insight into the Sialotranscriptome of the Cat Flea, Ctenocephalides felis
Article Snippet: The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech). .. The percentage of recombinant clones was determined by blue-white selection screening on LB/MgSO4 plates containing X-gal/IPTG.

Article Title: An insight into the sialome of the oriental rat flea, Xenopsylla cheopis (Rots)
Article Snippet: The packaged library was plated by infecting log phase XL1 Blue E. coli cells (Clontech, Palo Alto, CA). .. The percentage of recombinant clones was determined by performing a blue-white selection screening on LB/MgSO4 plates containing X-gal/IPTG.

Article Title: The sialotranscriptome of Antricola delacruzi female ticks is compatible with non-hematophagous behavior and an alternative source of food
Article Snippet: The packaged library was plated by infecting log phase XL1-Blue E. coli cells (Clontech, Palo Alto, CA). .. The percentage of recombinant clones was determined by performing a blue–white selection screening on LB/MgSO4 plates containing X-gal/IPTG.

Article Title: An Insight into the Sialotranscriptome of Simulium nigrimanum, a Black Fly Associated with Fogo Selvagem in South America
Article Snippet: The packaged library was plated by infecting log-phase XL1- Blue Escherichia coli cells (Clontech). .. The percentage of recombinant clones was determined by blue-white selection screening on LB/MgSO4 plates containing X-gal/IPTG.

Article Title: An insight into the sialotranscriptome of the brown dog tick, Rhipicephalus sanguineus
Article Snippet: The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech). .. The percentage of recombinant clones was determined by performing a blue-white selection screening on LB/MgSO4 plates containing X-gal/IPTG.

Article Title: Insight into the Salivary Transcriptome and Proteome of Dipetalogaster maxima
Article Snippet: The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech). .. The percentage of recombinant clones was determined by blue-white selection screening on LB/MgSO4 plates containing X-gal/IPTG.

Article Title: An insight into the sialome of the bed bug, Cimex lectularius
Article Snippet: The packaged library was plated by infecting log-phase XL1- Blue Escherichia coli cells (Clontech). .. The percentage of recombinant clones was determined by blue-white selection screening on LB/MgSO4 plates containing X-gal/IPTG.

Plasmid Preparation:

Article Title: An Insight into the Sialome of the Black Fly, Simulium vittatum
Article Snippet: The cDNA mixture was ligated into the λ TriplEx2 vector (Clontech), and the resulting ligation mixture was packaged using the GigaPack® III Plus packaging extract (Stratagene, La Jolla, CA) according to the manufacturer’s instructions. .. The packaged library was plated by infecting log-phase XL1- Blue Escherichia coli cells (Clontech).

Article Title: An Insight into the Sialotranscriptome of Triatoma matogrossensis, a Kissing Bug Associated with Fogo Selvagem in South America
Article Snippet: The concentrated cDNA was then ligated into a λ TriplEx2 vector (Clontech), and the resulting ligation mixture was packaged using GigaPack Gold III Plus packaging extract (Stratagene, La Jolla, CA) according to the manufacturer's instructions. .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: An insight into the sialome of Simulium guianense (DIPTERA:SIMulIIDAE), the main vector of River Blindness Disease in Brazil
Article Snippet: The concentrated cDNA mixture was ligated into the λ TriplEx2 vector, and the resulting ligation mixture was packaged using the GigaPack® III Plus packaging extract (Stratagene, La Jolla, CA) according to the manufacturer's instructions. .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: An insight into the salivary transcriptome and proteome of the soft tick and vector of epizootic bovine abortion, Ornithodoros coriaceus
Article Snippet: The cDNA mixture was ligated into the λ TriplEx2 vector (Clontech), and the resulting ligation mixture was packaged using the GigaPack® III Plus packaging extract (Stratagene, La Jolla, CA) according to the manufacturer’s instructions. .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: An Insight Into the Sialotranscriptome of Triatoma rubida (Hemiptera: Heteroptera)
Article Snippet: Concentrated cDNA was then ligated into a λ TriplEx2 vector (Clontech), and the resulting ligation mixture was packaged using GigaPack Gold III Plus packaging extract (Agilent Technologies, Santa Clara, CA) according to the manufacturer’s instructions. .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: An insight into the sialotranscriptome of the seed-feeding bug, Oncopeltus fasciatus
Article Snippet: The cDNAs were then ligated into a λ TriplEx2 vector (Clontech), and the resulting ligation mixture was packaged using GigaPack® III Plus packaging extract (Stratagene) according to the manufacturer's instructions. .. The packaged library was plated by infecting log-phase XL1-Blue E. coli cells (Clontech).

Article Title: Differential Expression Profiles in the Midgut of Triatoma infestans Infected with Trypanosoma cruzi
Article Snippet: The cDNA was precipitated and ligated into a λTriplEx2 vector (Clontech), and the ligation was packaged using GigaPack Gold III Plus packaging extract (Stratagene) according to the manufacturer's guidelines. .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech) for cDNA library amplification and titering unamplified and amplified libraries.

Article Title: An Insight into the Sialotranscriptome of the Cat Flea, Ctenocephalides felis
Article Snippet: The concentrated cDNA mixture was ligated into the λ TriplEx2 vector, and the resulting ligation mixture was packaged using the GigaPack® III Plus packaging extract (Stratagene, La Jolla, CA, USA) according to the manufacturer's instructions. .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: An insight into the sialome of the oriental rat flea, Xenopsylla cheopis (Rots)
Article Snippet: The cDNA mixture was ligated into the λ TriplEx2 vector (Clontech, Palo Alto, CA) and the resulting ligation mixture was packaged using the GigaPack® III Plus packaging extract (Stratagene, La Jolla, CA) according to the manufacturer's instructions. .. The packaged library was plated by infecting log phase XL1 Blue E. coli cells (Clontech, Palo Alto, CA).

Article Title: The sialotranscriptome of Antricola delacruzi female ticks is compatible with non-hematophagous behavior and an alternative source of food
Article Snippet: The cDNA mixture was ligated into the TriplEx2 vector (Clontech, Palo Alto, CA) and the resulting ligation mixture was packaged using the GigaPacks III Plus packaging extract (Stratagene, La Jolla, CA) according to the manufacturer’s instructions. .. The packaged library was plated by infecting log phase XL1-Blue E. coli cells (Clontech, Palo Alto, CA).

Article Title: An Insight into the Sialotranscriptome of Simulium nigrimanum, a Black Fly Associated with Fogo Selvagem in South America
Article Snippet: The concentrated cDNA mixture was ligated into the λ TriplEx2 vector (Clontech), and the resulting ligation mixture was packaged using the GigaPack III Plus packaging extract (Stratagene, La Jolla, CA) according to the manufacturer's instructions. .. The packaged library was plated by infecting log-phase XL1- Blue Escherichia coli cells (Clontech).

Article Title: An insight into the sialotranscriptome of the brown dog tick, Rhipicephalus sanguineus
Article Snippet: The cDNA mixture was ligated into the λ TriplEx2 vector (Clontech) and the resulting ligation mixture was packaged using the GigaPack® III Plus packaging extract (Stratagene, La Jolla, CA) according to the manufacturer's instructions. .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: Insight into the Salivary Transcriptome and Proteome of Dipetalogaster maxima
Article Snippet: Concentrated cDNA was then ligated into a λ TriplEx2 vector (Clontech), and the resulting ligation mixture was packaged using GigaPack® Gold III Plus packaging extract (Stratagene, La Jolla, CA, USA) according to the manufacturer’s instructions. .. The packaged library was plated by infecting log-phase XL1-Blue Escherichia coli cells (Clontech).

Article Title: An insight into the sialome of the bed bug, Cimex lectularius
Article Snippet: The cDNA mixture was ligated into the λ TriplEx2 vector (Clontech), and the resulting ligation mixture was packaged using the GigaPack® III Plus packaging extract (Stratagene, La Jolla, CA) according to the manufacturer's instructions. .. The packaged library was plated by infecting log-phase XL1- Blue Escherichia coli cells (Clontech).

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    TaKaRa log phase xl1 blue e coli cells
    Log Phase Xl1 Blue E Coli Cells, supplied by TaKaRa, used in various techniques. Bioz Stars score: 87/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/log phase xl1 blue e coli cells/product/TaKaRa
    Average 87 stars, based on 16 article reviews
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