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Stratagene log phase e coli xl1 blue cells
Log Phase E Coli Xl1 Blue Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/log phase e coli xl1 blue cells/product/Stratagene
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
log phase e coli xl1 blue cells - by Bioz Stars, 2020-04
86/100 stars

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Clone Assay:

Article Title: Differential gene expression in abdomens of the malaria vector mosquito, Anopheles gambiae, after sugar feeding, blood feeding and Plasmodium berghei infection
Article Snippet: These cDNAs were directionally cloned into Sfi I digested TripIEx2 (Clontech), and packaged using Gigapack III Gold Packaging Extract (Stratagene) according to the protocol provided. .. Packaged recombinant phages were incubated with log phase E. coli XL1-Blue cells (Stratagene), plated and library titers determined.

Article Title: Comparative Analysis of the Global Transcriptome of Anopheles funestus from Mali, West Africa
Article Snippet: First-strand cDNA synthesis was carried out at 42°C for 1 h using Superscript II Reverse Transcriptase (Life Science Technology, MD) with a modified oligo (dT) primer, CDS III (3′) containing a Sfi IB restriction site, and an additional primer (SMART III) that adds an Sfi IA restriction site at the 5′ end of the cDNA for directional cloning. .. Ligation mixtures were packaged using Gigapack III Gold Packaging Extract (Stratagene, La Jolla, CA) and incubated with log phase E. coli XL1-Blue cells (Stratagene).

Amplification:

Article Title: Differential gene expression in abdomens of the malaria vector mosquito, Anopheles gambiae, after sugar feeding, blood feeding and Plasmodium berghei infection
Article Snippet: Following proteinase K digestion and phenol:chloroform extraction, the amplified cDNAs were digested with 10 μl Sfi I (20 U/μl) at 50°C for 2 h and size fractionated using CHROMA SPIN-400 columns (Clontech). .. Packaged recombinant phages were incubated with log phase E. coli XL1-Blue cells (Stratagene), plated and library titers determined.

Article Title: Comparative Analysis of the Global Transcriptome of Anopheles funestus from Mali, West Africa
Article Snippet: Following proteinase K digestion and phenol:chloroform extraction, the amplified cDNAs were digested with Sfi I at 50°C for 2 h and size fractionated using CHROMA SPIN-400 columns (Clontech). .. Ligation mixtures were packaged using Gigapack III Gold Packaging Extract (Stratagene, La Jolla, CA) and incubated with log phase E. coli XL1-Blue cells (Stratagene).

Agarose Gel Electrophoresis:

Article Title: Comparative Analysis of the Global Transcriptome of Anopheles funestus from Mali, West Africa
Article Snippet: Fractions containing cDNAs longer than 500 bp, as judged by 1% agarose gel electrophoresis, were pooled, ethanol precipitated, and ligated into λTripIEx2 (Clontech). .. Ligation mixtures were packaged using Gigapack III Gold Packaging Extract (Stratagene, La Jolla, CA) and incubated with log phase E. coli XL1-Blue cells (Stratagene).

Ligation:

Article Title: Comparative Analysis of the Global Transcriptome of Anopheles funestus from Mali, West Africa
Article Snippet: .. Ligation mixtures were packaged using Gigapack III Gold Packaging Extract (Stratagene, La Jolla, CA) and incubated with log phase E. coli XL1-Blue cells (Stratagene). .. Unamplified library titer was estimated at 1.4×106 independent clones.

Isolation:

Article Title: Differential gene expression in abdomens of the malaria vector mosquito, Anopheles gambiae, after sugar feeding, blood feeding and Plasmodium berghei infection
Article Snippet: Packaged recombinant phages were incubated with log phase E. coli XL1-Blue cells (Stratagene), plated and library titers determined. .. White plaques were isolated and recombinant phages eluted overnight in 100 l SM buffer (0.1 M NaCl, 0.01 M MgSO4 .7H2 O, 0.05 M Tris-HCl (pH 7.5), 0.01% (w/v) gelatin).

Article Title: Comparative Analysis of the Global Transcriptome of Anopheles funestus from Mali, West Africa
Article Snippet: From total RNA isolated with Trizol (Molecular Research Center, Inc), mRNA was extracted using the PolyATract mRNA Isolation System (Promega) and converted to cDNA based on the SMART cDNA library construction kit (Clontech, Palo Alto, CA). .. Ligation mixtures were packaged using Gigapack III Gold Packaging Extract (Stratagene, La Jolla, CA) and incubated with log phase E. coli XL1-Blue cells (Stratagene).

IA:

Article Title: Comparative Analysis of the Global Transcriptome of Anopheles funestus from Mali, West Africa
Article Snippet: First-strand cDNA synthesis was carried out at 42°C for 1 h using Superscript II Reverse Transcriptase (Life Science Technology, MD) with a modified oligo (dT) primer, CDS III (3′) containing a Sfi IB restriction site, and an additional primer (SMART III) that adds an Sfi IA restriction site at the 5′ end of the cDNA for directional cloning. .. Ligation mixtures were packaged using Gigapack III Gold Packaging Extract (Stratagene, La Jolla, CA) and incubated with log phase E. coli XL1-Blue cells (Stratagene).

Incubation:

Article Title: Differential gene expression in abdomens of the malaria vector mosquito, Anopheles gambiae, after sugar feeding, blood feeding and Plasmodium berghei infection
Article Snippet: .. Packaged recombinant phages were incubated with log phase E. coli XL1-Blue cells (Stratagene), plated and library titers determined. ..

Article Title: Comparative Analysis of the Global Transcriptome of Anopheles funestus from Mali, West Africa
Article Snippet: .. Ligation mixtures were packaged using Gigapack III Gold Packaging Extract (Stratagene, La Jolla, CA) and incubated with log phase E. coli XL1-Blue cells (Stratagene). .. Unamplified library titer was estimated at 1.4×106 independent clones.

Polymerase Chain Reaction:

Article Title: Differential gene expression in abdomens of the malaria vector mosquito, Anopheles gambiae, after sugar feeding, blood feeding and Plasmodium berghei infection
Article Snippet: They appeared as homogeneous smears ranging from 100 bp to 5 kb. mRNA aliquots not used for cDNA synthesis and cloning were also subjected to PCR amplification using Taq Polymerase and visualized on the same gel. .. Packaged recombinant phages were incubated with log phase E. coli XL1-Blue cells (Stratagene), plated and library titers determined.

Article Title: Comparative Analysis of the Global Transcriptome of Anopheles funestus from Mali, West Africa
Article Snippet: Second-strand synthesis was conducted in the presence of both primers using Advantage 2 Polymerase Mix (Clontech), under the following PCR conditions: 95°C for 20 s, followed by 22 cycles of 95°C for 5 s and 68°C for 6 min, concluding at 72°C for 10 min. .. Ligation mixtures were packaged using Gigapack III Gold Packaging Extract (Stratagene, La Jolla, CA) and incubated with log phase E. coli XL1-Blue cells (Stratagene).

cDNA Library Assay:

Article Title: Differential gene expression in abdomens of the malaria vector mosquito, Anopheles gambiae, after sugar feeding, blood feeding and Plasmodium berghei infection
Article Snippet: Paragraph title: cDNA library construction ... Packaged recombinant phages were incubated with log phase E. coli XL1-Blue cells (Stratagene), plated and library titers determined.

Article Title: Comparative Analysis of the Global Transcriptome of Anopheles funestus from Mali, West Africa
Article Snippet: Paragraph title: cDNA Library Construction ... Ligation mixtures were packaged using Gigapack III Gold Packaging Extract (Stratagene, La Jolla, CA) and incubated with log phase E. coli XL1-Blue cells (Stratagene).

Modification:

Article Title: Comparative Analysis of the Global Transcriptome of Anopheles funestus from Mali, West Africa
Article Snippet: First-strand cDNA synthesis was carried out at 42°C for 1 h using Superscript II Reverse Transcriptase (Life Science Technology, MD) with a modified oligo (dT) primer, CDS III (3′) containing a Sfi IB restriction site, and an additional primer (SMART III) that adds an Sfi IA restriction site at the 5′ end of the cDNA for directional cloning. .. Ligation mixtures were packaged using Gigapack III Gold Packaging Extract (Stratagene, La Jolla, CA) and incubated with log phase E. coli XL1-Blue cells (Stratagene).

Recombinant:

Article Title: Differential gene expression in abdomens of the malaria vector mosquito, Anopheles gambiae, after sugar feeding, blood feeding and Plasmodium berghei infection
Article Snippet: .. Packaged recombinant phages were incubated with log phase E. coli XL1-Blue cells (Stratagene), plated and library titers determined. ..

Plasmid Preparation:

Article Title: Differential gene expression in abdomens of the malaria vector mosquito, Anopheles gambiae, after sugar feeding, blood feeding and Plasmodium berghei infection
Article Snippet: Packaged recombinant phages were incubated with log phase E. coli XL1-Blue cells (Stratagene), plated and library titers determined. .. The inserts were amplified via PCR using 5' and 3' vector specific primers; 5' LD Amplimer Primer, 5'-CTCGGGAAGCGCGCCATTGTGTTGG-3' and 3' LD Amplimer Primer, 5'-ATACGACTCACTATAGGGCGAATTGGC-3' (Invitrogen).

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    Stratagene log phase xl1 blue e coli cells
    Log Phase Xl1 Blue E Coli Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/log phase xl1 blue e coli cells/product/Stratagene
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    log phase xl1 blue e coli cells - by Bioz Stars, 2020-04
    93/100 stars
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