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Illumina Inc locus specific oligos
Locus Specific Oligos, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/locus specific oligos/product/Illumina Inc
Average 94 stars, based on 3 article reviews
Price from $9.99 to $1999.99
locus specific oligos - by Bioz Stars, 2020-04
94/100 stars

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Related Articles

Clone Assay:

Article Title: Construction of genetic linkage map and identification of a novel major locus for resistance to pine wood nematode in Japanese black pine (Pinus thunbergii)
Article Snippet: The 768 candidate genes and SNPs supplied on each platform were selected by gene expression profiling between resistant and susceptible clones (Hirao et al. unpublished), and special attention was paid to select only one SNP per differentially expressed transcript. .. For Illumina’s GoldenGate platform, the 768 selected SNPs were designed with the custom oligonucleotide pooled assay (OPA; Illumina Inc., San Diego, CA) containing the allele-specific and locus-specific oligos for use in the Illumina GoldenGate assay.

Multiplex Assay:

Article Title: Genetic variation in APOJ, LPL, and TNFRSF10B affects plasma fatty acid distribution in Alaskan Eskimos 1 affects plasma fatty acid distribution in Alaskan Eskimos 1 2 affects plasma fatty acid distribution in Alaskan Eskimos 1 2 3
Article Snippet: Single nucleotide polymorphisms (SNPs) were typed by using the multiplex VeraCode technology from Illumina according to the manufacturer's protocol (Illumina, San Diego, CA). .. Genomic DNA (250 ng) was activated chemically with biotin and then hybridized to a pool of locus-specific oligos (OPA, Oligo Pool All; Illumina).

Amplification:

Article Title: Allele-specific gene expression patterns in primary leukemic cells reveal regulation of gene expression by CpG site methylation
Article Snippet: A whole-genome amplified (WGA) DNA sample, where methylated C-residues are not replicated and the CpG sites remain unmethylated was used as a negative control for the bisulfite treatment and subsequent genotyping procedure. .. The allele- and locus-specific oligonucleotides were hybridized to the immobilized DNA, the allele-specific primers were extended with dNTPs and the extension products were ligated to the locus-specific oligonucleotides according to protocols from Illumina.

Sample Prep:

Article Title: Construction of genetic linkage map and identification of a novel major locus for resistance to pine wood nematode in Japanese black pine (Pinus thunbergii)
Article Snippet: Using a TruSeq RNA Sample Prep kit ver.2 (Illumina), cDNA synthesis from an RNA sample from each organ, nebulization, adaptor ligation (including index tagging for individual recognition), bridge PCR and paired-end sequencing were performed on the Illumina HiSeq 2500 platform. .. For Illumina’s GoldenGate platform, the 768 selected SNPs were designed with the custom oligonucleotide pooled assay (OPA; Illumina Inc., San Diego, CA) containing the allele-specific and locus-specific oligos for use in the Illumina GoldenGate assay.

Positive Control:

Article Title: Allele-specific gene expression patterns in primary leukemic cells reveal regulation of gene expression by CpG site methylation
Article Snippet: A DNA sample treated with SssI methyltransferase to methylate all CpG sites was used as a positive control for the methylation assay. .. The allele- and locus-specific oligonucleotides were hybridized to the immobilized DNA, the allele-specific primers were extended with dNTPs and the extension products were ligated to the locus-specific oligonucleotides according to protocols from Illumina.

Fluorescence:

Article Title: Application of a New Method for GWAS in a Related Case/Control Sample with Known Pedigree Structure: Identification of New Loci for Nephrolithiasis
Article Snippet: Genomic DNA (250 ng) was activated chemically with biotin and then hybridized to a pool of locus-specific oligos (OPA, OligoPool All; Illumina). .. Raw data, consisting of intensities of fluorescence, were then imported into the analysis software GenomeStudio and the automatic allele calling was done using GeneCall threshold of 0.25.

Article Title: Allele-specific gene expression patterns in primary leukemic cells reveal regulation of gene expression by CpG site methylation
Article Snippet: The allele- and locus-specific oligonucleotides were hybridized to the immobilized DNA, the allele-specific primers were extended with dNTPs and the extension products were ligated to the locus-specific oligonucleotides according to protocols from Illumina. .. The fluorescence signals were measured from the BeadArrays using an Illumina BeadStation GX scanner.

Ligation:

Article Title: Construction of genetic linkage map and identification of a novel major locus for resistance to pine wood nematode in Japanese black pine (Pinus thunbergii)
Article Snippet: Using a TruSeq RNA Sample Prep kit ver.2 (Illumina), cDNA synthesis from an RNA sample from each organ, nebulization, adaptor ligation (including index tagging for individual recognition), bridge PCR and paired-end sequencing were performed on the Illumina HiSeq 2500 platform. .. For Illumina’s GoldenGate platform, the 768 selected SNPs were designed with the custom oligonucleotide pooled assay (OPA; Illumina Inc., San Diego, CA) containing the allele-specific and locus-specific oligos for use in the Illumina GoldenGate assay.

Article Title: Allele-specific gene expression patterns in primary leukemic cells reveal regulation of gene expression by CpG site methylation
Article Snippet: The allele- and locus-specific oligonucleotides were hybridized to the immobilized DNA, the allele-specific primers were extended with dNTPs and the extension products were ligated to the locus-specific oligonucleotides according to protocols from Illumina. .. The DNA templates created by primer extension and ligation were amplified by PCR with fluorescently labeled universal primers complementary to sequences in the allele- and locus-specific oligonucleotides.

Methylation:

Article Title: Allele-specific gene expression patterns in primary leukemic cells reveal regulation of gene expression by CpG site methylation
Article Snippet: A DNA sample treated with SssI methyltransferase to methylate all CpG sites was used as a positive control for the methylation assay. .. The allele- and locus-specific oligonucleotides were hybridized to the immobilized DNA, the allele-specific primers were extended with dNTPs and the extension products were ligated to the locus-specific oligonucleotides according to protocols from Illumina.

Generated:

Article Title: High-Throughput SNP Discovery and Genotyping for Constructing a Saturated Linkage Map of Chickpea (Cicer arietinum L.)
Article Snippet: .. Thus, SNPs having the highest ADT scores were selected for designing the custom Oligo Pool All (CpOPA) containing the allele-specific and locus-specific oligos for use in the Illumina GoldenGate assay and the first chickpea 768-OPA was generated and designated CpOPA-I. ..

Labeling:

Article Title: Allele-specific gene expression patterns in primary leukemic cells reveal regulation of gene expression by CpG site methylation
Article Snippet: The allele- and locus-specific oligonucleotides were hybridized to the immobilized DNA, the allele-specific primers were extended with dNTPs and the extension products were ligated to the locus-specific oligonucleotides according to protocols from Illumina. .. The DNA templates created by primer extension and ligation were amplified by PCR with fluorescently labeled universal primers complementary to sequences in the allele- and locus-specific oligonucleotides.

Goldengate Assay:

Article Title: Application of a New Method for GWAS in a Related Case/Control Sample with Known Pedigree Structure: Identification of New Loci for Nephrolithiasis
Article Snippet: SNPs were typed by using the VeraCode GoldenGate Genotyping Assay from Illumina according to the manufacturer's protocol (Illumina, San Diego, CA). .. Genomic DNA (250 ng) was activated chemically with biotin and then hybridized to a pool of locus-specific oligos (OPA, OligoPool All; Illumina).

Article Title: High-Throughput SNP Discovery and Genotyping for Constructing a Saturated Linkage Map of Chickpea (Cicer arietinum L.)
Article Snippet: .. Thus, SNPs having the highest ADT scores were selected for designing the custom Oligo Pool All (CpOPA) containing the allele-specific and locus-specific oligos for use in the Illumina GoldenGate assay and the first chickpea 768-OPA was generated and designated CpOPA-I. ..

Article Title: Construction of genetic linkage map and identification of a novel major locus for resistance to pine wood nematode in Japanese black pine (Pinus thunbergii)
Article Snippet: .. For Illumina’s GoldenGate platform, the 768 selected SNPs were designed with the custom oligonucleotide pooled assay (OPA; Illumina Inc., San Diego, CA) containing the allele-specific and locus-specific oligos for use in the Illumina GoldenGate assay. .. Genotyping of the SNP markers was carried out using the 768-OPA and Illumina’s BeadArray Express Reader according to the standard manufacturer’s protocol.

Article Title: Allele-specific gene expression patterns in primary leukemic cells reveal regulation of gene expression by CpG site methylation
Article Snippet: The GoldenGate assay uses two allele-specific oligonucleotides and two locus-specific oligonucleotides for each CpG site. .. The allele- and locus-specific oligonucleotides were hybridized to the immobilized DNA, the allele-specific primers were extended with dNTPs and the extension products were ligated to the locus-specific oligonucleotides according to protocols from Illumina.

Genotyping Assay:

Article Title: High-Throughput SNP Discovery and Genotyping for Constructing a Saturated Linkage Map of Chickpea (Cicer arietinum L.)
Article Snippet: This score serves as a reliability metric for testing whether or not a particular SNP will convert into a successful working GoldenGate assay where SNPs with the scores of 0.6 or higher possess a high probability of converting into a successful genotyping assay. .. Thus, SNPs having the highest ADT scores were selected for designing the custom Oligo Pool All (CpOPA) containing the allele-specific and locus-specific oligos for use in the Illumina GoldenGate assay and the first chickpea 768-OPA was generated and designated CpOPA-I.

Bridge PCR:

Article Title: Construction of genetic linkage map and identification of a novel major locus for resistance to pine wood nematode in Japanese black pine (Pinus thunbergii)
Article Snippet: Using a TruSeq RNA Sample Prep kit ver.2 (Illumina), cDNA synthesis from an RNA sample from each organ, nebulization, adaptor ligation (including index tagging for individual recognition), bridge PCR and paired-end sequencing were performed on the Illumina HiSeq 2500 platform. .. For Illumina’s GoldenGate platform, the 768 selected SNPs were designed with the custom oligonucleotide pooled assay (OPA; Illumina Inc., San Diego, CA) containing the allele-specific and locus-specific oligos for use in the Illumina GoldenGate assay.

Polymerase Chain Reaction:

Article Title: Application of a New Method for GWAS in a Related Case/Control Sample with Known Pedigree Structure: Identification of New Loci for Nephrolithiasis
Article Snippet: Genomic DNA (250 ng) was activated chemically with biotin and then hybridized to a pool of locus-specific oligos (OPA, OligoPool All; Illumina). .. After removal of nonspecific unbound oligos, a PCR reaction was performed by using fluorescent-labeled primers (Cy3 and Cy5).

Article Title: Genetic variation in APOJ, LPL, and TNFRSF10B affects plasma fatty acid distribution in Alaskan Eskimos 1 affects plasma fatty acid distribution in Alaskan Eskimos 1 2 affects plasma fatty acid distribution in Alaskan Eskimos 1 2 3
Article Snippet: Genomic DNA (250 ng) was activated chemically with biotin and then hybridized to a pool of locus-specific oligos (OPA, Oligo Pool All; Illumina). .. After removal of nonspecific unbound oligos, a PCR reaction was performed by using fluorescent-labeled primers (Cy3 and Cy5).

Article Title: Allele-specific gene expression patterns in primary leukemic cells reveal regulation of gene expression by CpG site methylation
Article Snippet: The allele- and locus-specific oligonucleotides were hybridized to the immobilized DNA, the allele-specific primers were extended with dNTPs and the extension products were ligated to the locus-specific oligonucleotides according to protocols from Illumina. .. The DNA templates created by primer extension and ligation were amplified by PCR with fluorescently labeled universal primers complementary to sequences in the allele- and locus-specific oligonucleotides.

Negative Control:

Article Title: Allele-specific gene expression patterns in primary leukemic cells reveal regulation of gene expression by CpG site methylation
Article Snippet: A whole-genome amplified (WGA) DNA sample, where methylated C-residues are not replicated and the CpG sites remain unmethylated was used as a negative control for the bisulfite treatment and subsequent genotyping procedure. .. The allele- and locus-specific oligonucleotides were hybridized to the immobilized DNA, the allele-specific primers were extended with dNTPs and the extension products were ligated to the locus-specific oligonucleotides according to protocols from Illumina.

Expressing:

Article Title: Construction of genetic linkage map and identification of a novel major locus for resistance to pine wood nematode in Japanese black pine (Pinus thunbergii)
Article Snippet: The 768 candidate genes and SNPs supplied on each platform were selected by gene expression profiling between resistant and susceptible clones (Hirao et al. unpublished), and special attention was paid to select only one SNP per differentially expressed transcript. .. For Illumina’s GoldenGate platform, the 768 selected SNPs were designed with the custom oligonucleotide pooled assay (OPA; Illumina Inc., San Diego, CA) containing the allele-specific and locus-specific oligos for use in the Illumina GoldenGate assay.

Sequencing:

Article Title: High-Throughput SNP Discovery and Genotyping for Constructing a Saturated Linkage Map of Chickpea (Cicer arietinum L.)
Article Snippet: For developing the GGGT assay, the identified SNPs along with the 60 bp sequence flanking it on either side were submitted to Illumina for processing by Illumina's Array Design Tool (ADT) in order to obtain a designability rank score for each SNP ranging from 0 to 1. .. Thus, SNPs having the highest ADT scores were selected for designing the custom Oligo Pool All (CpOPA) containing the allele-specific and locus-specific oligos for use in the Illumina GoldenGate assay and the first chickpea 768-OPA was generated and designated CpOPA-I.

Article Title: Construction of genetic linkage map and identification of a novel major locus for resistance to pine wood nematode in Japanese black pine (Pinus thunbergii)
Article Snippet: Using a TruSeq RNA Sample Prep kit ver.2 (Illumina), cDNA synthesis from an RNA sample from each organ, nebulization, adaptor ligation (including index tagging for individual recognition), bridge PCR and paired-end sequencing were performed on the Illumina HiSeq 2500 platform. .. For Illumina’s GoldenGate platform, the 768 selected SNPs were designed with the custom oligonucleotide pooled assay (OPA; Illumina Inc., San Diego, CA) containing the allele-specific and locus-specific oligos for use in the Illumina GoldenGate assay.

Whole Genome Amplification:

Article Title: Allele-specific gene expression patterns in primary leukemic cells reveal regulation of gene expression by CpG site methylation
Article Snippet: A whole-genome amplified (WGA) DNA sample, where methylated C-residues are not replicated and the CpG sites remain unmethylated was used as a negative control for the bisulfite treatment and subsequent genotyping procedure. .. The allele- and locus-specific oligonucleotides were hybridized to the immobilized DNA, the allele-specific primers were extended with dNTPs and the extension products were ligated to the locus-specific oligonucleotides according to protocols from Illumina.

DNA Methylation Assay:

Article Title: Allele-specific gene expression patterns in primary leukemic cells reveal regulation of gene expression by CpG site methylation
Article Snippet: Paragraph title: Analysis of DNA methylation ... The allele- and locus-specific oligonucleotides were hybridized to the immobilized DNA, the allele-specific primers were extended with dNTPs and the extension products were ligated to the locus-specific oligonucleotides according to protocols from Illumina.

Software:

Article Title: Application of a New Method for GWAS in a Related Case/Control Sample with Known Pedigree Structure: Identification of New Loci for Nephrolithiasis
Article Snippet: Genomic DNA (250 ng) was activated chemically with biotin and then hybridized to a pool of locus-specific oligos (OPA, OligoPool All; Illumina). .. PCR products were cleaned and denatured, and single-stranded fluorescent-labeled DNAs were hybridized to VeraCode beads, which were scanned on a BeadXpress reader by using Illumina VeraScan V1.1 software.

Article Title: High-Throughput SNP Discovery and Genotyping for Constructing a Saturated Linkage Map of Chickpea (Cicer arietinum L.)
Article Snippet: Thus, SNPs having the highest ADT scores were selected for designing the custom Oligo Pool All (CpOPA) containing the allele-specific and locus-specific oligos for use in the Illumina GoldenGate assay and the first chickpea 768-OPA was generated and designated CpOPA-I. .. The automatic allele calling for each locus was inferred with the GenomeStudio Software V2011.1 (Illumina).

Article Title: Construction of genetic linkage map and identification of a novel major locus for resistance to pine wood nematode in Japanese black pine (Pinus thunbergii)
Article Snippet: Then, the reads of each library were mapped to reference sequences by BWA [ ] and SNPs were identified using SAMtools software [ ] with default settings. .. For Illumina’s GoldenGate platform, the 768 selected SNPs were designed with the custom oligonucleotide pooled assay (OPA; Illumina Inc., San Diego, CA) containing the allele-specific and locus-specific oligos for use in the Illumina GoldenGate assay.

Article Title: Genetic variation in APOJ, LPL, and TNFRSF10B affects plasma fatty acid distribution in Alaskan Eskimos 1 affects plasma fatty acid distribution in Alaskan Eskimos 1 2 affects plasma fatty acid distribution in Alaskan Eskimos 1 2 3
Article Snippet: Genomic DNA (250 ng) was activated chemically with biotin and then hybridized to a pool of locus-specific oligos (OPA, Oligo Pool All; Illumina). .. All steps through the PCR were performed on a Tecan Freedom EVO 150cm liquid handler (Tecan, Männedorf, Switzerland) with Illumina GTS Robot Control software (Illumina).

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    Illumina Inc locus specific oligos
    Locus Specific Oligos, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/locus specific oligos/product/Illumina Inc
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    locus specific oligos - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

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