lmw dna ladder  (New England Biolabs)


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    Name:
    Low Molecular Weight DNA Ladder
    Description:
    Low Molecular Weight DNA Ladder 500 gel lanes
    Catalog Number:
    n3233l
    Price:
    270
    Category:
    DNA Ladders
    Size:
    500 gel lanes
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    Structured Review

    New England Biolabs lmw dna ladder
    Low Molecular Weight DNA Ladder
    Low Molecular Weight DNA Ladder 500 gel lanes
    https://www.bioz.com/result/lmw dna ladder/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lmw dna ladder - by Bioz Stars, 2021-03
    94/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Discovery and profiling of small RNAs responsive to stress conditions in the plant pathogen Pectobacterium atrosepticum
    Article Snippet: Thermal cycling conditions were: 95 °C for 2 min; 30 cycles of 95 °C for 30 sec, 57 °C for 30 sec, 72 °C for 60 sec, and the final extension at 72 °C for 5 min. .. The PCR products were analysed on 1.5 % agarose gel including 100 bp DNA molecular weight ladder (NEB, UK). .. Differential expression analysis of sRNAs Artemis genome browser was used to create features of the discovered sRNAs on the P. atrosepticum reference genome and to make read counts for reads aligning to each strand under each growth condition.

    Agarose Gel Electrophoresis:

    Article Title: Discovery and profiling of small RNAs responsive to stress conditions in the plant pathogen Pectobacterium atrosepticum
    Article Snippet: Thermal cycling conditions were: 95 °C for 2 min; 30 cycles of 95 °C for 30 sec, 57 °C for 30 sec, 72 °C for 60 sec, and the final extension at 72 °C for 5 min. .. The PCR products were analysed on 1.5 % agarose gel including 100 bp DNA molecular weight ladder (NEB, UK). .. Differential expression analysis of sRNAs Artemis genome browser was used to create features of the discovered sRNAs on the P. atrosepticum reference genome and to make read counts for reads aligning to each strand under each growth condition.

    Molecular Weight:

    Article Title: Discovery and profiling of small RNAs responsive to stress conditions in the plant pathogen Pectobacterium atrosepticum
    Article Snippet: Thermal cycling conditions were: 95 °C for 2 min; 30 cycles of 95 °C for 30 sec, 57 °C for 30 sec, 72 °C for 60 sec, and the final extension at 72 °C for 5 min. .. The PCR products were analysed on 1.5 % agarose gel including 100 bp DNA molecular weight ladder (NEB, UK). .. Differential expression analysis of sRNAs Artemis genome browser was used to create features of the discovered sRNAs on the P. atrosepticum reference genome and to make read counts for reads aligning to each strand under each growth condition.

    Article Title: Detection of the pediocin gene pedA in strains from human faeces by real-time PCR and characterization of Pediococcus acidilactici UVA1
    Article Snippet: PCR products were separated on a 2 % agarose gel in 1 × TAE buffer by electrophoresis at 90 V for 2 h 30. .. The low molecular weight DNA ladder and Tridye 100 bp DNA-ladder (New England BioLabs, Ipswich, MA, USA) were used as size standards. ..

    Article Title: RIPiT-Seq: A high-throughput approach for footprinting RNA:protein complexes
    Article Snippet: To estimate the size-range and molar amounts of the purified RNA, we also 5′-end label oligonucleotide of known size and concentration and run them on the same gel. .. In separate, but otherwise identical reactions, also label 0.1 pmole of a synthetic RNA oligonucleotide of known length, 1 µl of low molecular weight ssDNA ladder and 1 µl of NEB 100 bp DNA ladder. ..

    Article Title: Effects of recombinant human canstatin protein in the treatment of pancreatic cancer
    Article Snippet: E. coli BL21 and plasmid expression vector pET-22b (+) were purchased from the College of Life Sciences of Fudan University. pUCm-T vector was purchased from Shenergy Biocolor. .. Bam HI, Hin dIII, T4 DNA ligase and DNA molecular weight marker were supplied by New England Biolabs. .. Protein marker was purchased from Shanghai Sangon Co. Trizol was provided by Jingmei Biotech.

    Article Title: Nisin H Is a New Nisin Variant Produced by the Gut-Derived Strain Streptococcus hyointestinalis DPC6484
    Article Snippet: .. Molecular fingerprinting of S. hyointestinalis isolates was performed by pulsed-field gel electrophoresis (PFGE) as described by Simpson et al. (2002) ( ) using SmaI restriction endonucleases and DNA molecular weight markers (9.42 to 242.50 kb; New England BioLabs, Beverly, MA). .. DNA fragments were resolved with a CHEF (contour-clamped homogeneous electric field) DRIII pulsed-field system (Bio-Rad Laboratories) at 6 V/cm for 18 h with a 1- to 30-s linear ramp time to resolve bands.

    Article Title: Switching the activity of Cas12a using guide RNA strand displacement circuits
    Article Snippet: .. For both purification methods, the purified RNA was quantified by running it on an 8 M urea denaturing TBE 12% polyacrylamide (29:1 acrylamide/bis-acrylamide) gel at 120 V for 45 min and compared with an RNA ladder of known concentration (RiboRuler Low Range RNA Ladder, ThermoFisher) to determine its concentration and with a DNA ladder (Low Molecular Weight DNA Ladder, New England Biolabs) to determine its length. .. Cutting assays for gRNAs AsCas12a (containing nuclear localization sequences and C-terminal His tags) was ordered from IDT with a concentration of around 64 μM.

    Article Title: Double Stranded Sperm DNA Breaks, Measured by Comet Assay, Are Associated with Unexplained Recurrent Miscarriage in Couples without a Female Factor
    Article Snippet: A quarter slice of each plug was cut off and placed on 1% gel and resolved by electrophoresis on a contour-clamped homogeneous electric field apparatus (Bio-Rad CHEF DRIII system) in TBE 0.5 X (Tris-borate 50 mM, EDTA 0.1 mM) at a 120° angle, 14°C, 4 V/cm and with the following pulses: 6.7 tp 33.7 seconds for 27.1 hours. .. DNA molecular weight markers consisting of Lambda ladder PFG marker and Low Range PFG Marker (New England Biolabs; Ipswich, MA, USA) were included in each electrophoretic run. .. Gels were stained with ethidium bromide and visualised and photographed under ultraviolet light using the GelDoc System (BioRad; Hercules, CA, USA).

    Article Title: Circularization restores signal recognition particle RNA functionality in Thermoproteus
    Article Snippet: The T. tenax RNA-Seq data is available at Gene Expression Omnibus (GSE72127, [ http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE72127 ]). .. Northern blot Fractionation of 1–4 µg T. tenax total RNA was performed by electrophoretic separation on 8% denaturing-polyacrylamide gels next to an RNA and DNA marker (low molecular weight DNA ladder, NEB). .. Additionally, to verify the circularization of SRP RNA, 15 ng of full-length SRP RNA in vitro transcript and circularized SRP RNA served as a running control.

    Marker:

    Article Title: Effects of recombinant human canstatin protein in the treatment of pancreatic cancer
    Article Snippet: E. coli BL21 and plasmid expression vector pET-22b (+) were purchased from the College of Life Sciences of Fudan University. pUCm-T vector was purchased from Shenergy Biocolor. .. Bam HI, Hin dIII, T4 DNA ligase and DNA molecular weight marker were supplied by New England Biolabs. .. Protein marker was purchased from Shanghai Sangon Co. Trizol was provided by Jingmei Biotech.

    Article Title: Double Stranded Sperm DNA Breaks, Measured by Comet Assay, Are Associated with Unexplained Recurrent Miscarriage in Couples without a Female Factor
    Article Snippet: A quarter slice of each plug was cut off and placed on 1% gel and resolved by electrophoresis on a contour-clamped homogeneous electric field apparatus (Bio-Rad CHEF DRIII system) in TBE 0.5 X (Tris-borate 50 mM, EDTA 0.1 mM) at a 120° angle, 14°C, 4 V/cm and with the following pulses: 6.7 tp 33.7 seconds for 27.1 hours. .. DNA molecular weight markers consisting of Lambda ladder PFG marker and Low Range PFG Marker (New England Biolabs; Ipswich, MA, USA) were included in each electrophoretic run. .. Gels were stained with ethidium bromide and visualised and photographed under ultraviolet light using the GelDoc System (BioRad; Hercules, CA, USA).

    Article Title: Circularization restores signal recognition particle RNA functionality in Thermoproteus
    Article Snippet: The T. tenax RNA-Seq data is available at Gene Expression Omnibus (GSE72127, [ http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE72127 ]). .. Northern blot Fractionation of 1–4 µg T. tenax total RNA was performed by electrophoretic separation on 8% denaturing-polyacrylamide gels next to an RNA and DNA marker (low molecular weight DNA ladder, NEB). .. Additionally, to verify the circularization of SRP RNA, 15 ng of full-length SRP RNA in vitro transcript and circularized SRP RNA served as a running control.

    Pulsed-Field Gel:

    Article Title: Nisin H Is a New Nisin Variant Produced by the Gut-Derived Strain Streptococcus hyointestinalis DPC6484
    Article Snippet: .. Molecular fingerprinting of S. hyointestinalis isolates was performed by pulsed-field gel electrophoresis (PFGE) as described by Simpson et al. (2002) ( ) using SmaI restriction endonucleases and DNA molecular weight markers (9.42 to 242.50 kb; New England BioLabs, Beverly, MA). .. DNA fragments were resolved with a CHEF (contour-clamped homogeneous electric field) DRIII pulsed-field system (Bio-Rad Laboratories) at 6 V/cm for 18 h with a 1- to 30-s linear ramp time to resolve bands.

    Purification:

    Article Title: Switching the activity of Cas12a using guide RNA strand displacement circuits
    Article Snippet: .. For both purification methods, the purified RNA was quantified by running it on an 8 M urea denaturing TBE 12% polyacrylamide (29:1 acrylamide/bis-acrylamide) gel at 120 V for 45 min and compared with an RNA ladder of known concentration (RiboRuler Low Range RNA Ladder, ThermoFisher) to determine its concentration and with a DNA ladder (Low Molecular Weight DNA Ladder, New England Biolabs) to determine its length. .. Cutting assays for gRNAs AsCas12a (containing nuclear localization sequences and C-terminal His tags) was ordered from IDT with a concentration of around 64 μM.

    Concentration Assay:

    Article Title: Switching the activity of Cas12a using guide RNA strand displacement circuits
    Article Snippet: .. For both purification methods, the purified RNA was quantified by running it on an 8 M urea denaturing TBE 12% polyacrylamide (29:1 acrylamide/bis-acrylamide) gel at 120 V for 45 min and compared with an RNA ladder of known concentration (RiboRuler Low Range RNA Ladder, ThermoFisher) to determine its concentration and with a DNA ladder (Low Molecular Weight DNA Ladder, New England Biolabs) to determine its length. .. Cutting assays for gRNAs AsCas12a (containing nuclear localization sequences and C-terminal His tags) was ordered from IDT with a concentration of around 64 μM.

    Northern Blot:

    Article Title: Circularization restores signal recognition particle RNA functionality in Thermoproteus
    Article Snippet: The T. tenax RNA-Seq data is available at Gene Expression Omnibus (GSE72127, [ http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE72127 ]). .. Northern blot Fractionation of 1–4 µg T. tenax total RNA was performed by electrophoretic separation on 8% denaturing-polyacrylamide gels next to an RNA and DNA marker (low molecular weight DNA ladder, NEB). .. Additionally, to verify the circularization of SRP RNA, 15 ng of full-length SRP RNA in vitro transcript and circularized SRP RNA served as a running control.

    Fractionation:

    Article Title: Circularization restores signal recognition particle RNA functionality in Thermoproteus
    Article Snippet: The T. tenax RNA-Seq data is available at Gene Expression Omnibus (GSE72127, [ http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE72127 ]). .. Northern blot Fractionation of 1–4 µg T. tenax total RNA was performed by electrophoretic separation on 8% denaturing-polyacrylamide gels next to an RNA and DNA marker (low molecular weight DNA ladder, NEB). .. Additionally, to verify the circularization of SRP RNA, 15 ng of full-length SRP RNA in vitro transcript and circularized SRP RNA served as a running control.

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