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Thermo Fisher lmg194
Lmg194, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lmg194/product/Thermo Fisher
Average 84 stars, based on 1 article reviews
Price from $9.99 to $1999.99
lmg194 - by Bioz Stars, 2020-02
84/100 stars

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Clone Assay:

Article Title: Bcl-2-mediated alterations in endoplasmic reticulum Ca2+ analyzed with an improved genetically encoded fluorescent sensor
Article Snippet: The constructs were cloned between the Bam HI/ Eco RI sites in pRSETB or pBAD for protein purification and pcDNA3 for expression in mammalian cells. .. For protein purification, cameleons were expressed in either JM109 (Stratagene) or LMG194 (Invitrogen) and grown over-night at 25°C.

Article Title: Purification and Characterization of PBP4a, a New Low-Molecular-Weight Penicillin-Binding Protein from Bacillus subtilis
Article Snippet: .. The cloning and expression experiments were performed with E. coli TG1 (Amersham Pharmacia Biotech, Roosendaal, The Netherlands), JM109, JM110 (Stratagene, Amsterdam Zuidoost, The Netherlands), Top 10, and LMG194 (Invitrogen, Groningen, The Netherlands) using pMK4 , a shuttle Bacillus-E. coli plasmid, or the pBAD/Myc-HisA (Invitrogen) vectors. .. The ampicillin resistance gene of the last plasmid was replaced by the kanamycin resistance gene using the following strategy.

Article Title: A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales
Article Snippet: Protein characterization in vitro For expression in bacteria, the miRFPs were cloned into pBAD/His-B vector (Life Technologies/Invitrogen). .. LMG194 or TOP10 host cells (Invitrogen) were used for protein expression.

Article Title: Cloning, expression, and characterization of a peptidoglycan hydrolase from the Burkholderia pseudomallei phage ST79
Article Snippet: Escherichia coli BL21 (DE3) was used as the host for cloning and protein expression processes (Thermo Fisher Scientific, Waltham, MA, USA). .. Eighteen Gram-negative bacteria, five of which were E. coli host strains; Top10, LMG194 (Invitrogen, CA, USA), DH5α, BL21 (DE3) and XL1-Blue (Thermo Fisher Scientific, Waltham, MA, USA), two B. pseudomallei isolates, P37 and G1; two Burkholderia mallei isolates, EY2233 and EY2237; Burkholderia thailandensis UE5 (kindly provided by MORU, Mahidol University, Thailand), Klebsiella pneumoniae , Vibrio parahaemolyticus , Pseudomonas vasculitis , P. aeruginosa , Acinetobacter baumannii , Salmonella gr.

Article Title: Vectors for ligation-independent construction of lacZ gene fusions and cloning of PCR products using a nicking endonuclease
Article Snippet: .. Promoters were cloned from E. coli K-12 strains, including LMG194 (F− mcrA , Δ[ mrr - hsdRMS-mcrBC ] Φ80 lacZ ΔM15, Δ lacX74 , recA1 , araD139 , Δ( ara-leu )7697, galU , galK , rpsL , endA1 , nupG ) (obtained from Invitrogen, Inc., Carlsbad, CA), or derivatives of Y. pestis strain CO92 ( ) deleted for either the pgm locus ( pgm ) ( ) or pCD1, the large virulence plasmid required for pathogenesis (Lcr− ) , enabling manipulation under BSL-2 laboratory conditions. .. E. coli NEB 5-alpha ( fhuA2 Δ (argF-lacZ)U169 phoA glnV44 Φ 80 Δ (lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 ) was obtained from New England Biolabs (NEB) (Ipswich, MA) and used as a host for all cloning experiments.

Article Title: Reconstruction of an Acetogenic 2,3-Butanediol Pathway Involving a Novel NADPH-Dependent Primary-Secondary Alcohol Dehydrogenase
Article Snippet: C. ragsdalei P11 (ATCC BAA-622), C. beijerinckii NCIMB 8052 (ATCC 51743), and C. acetobutylicum (ATCC 824) were from the American Type Culture Collection (ATCC), Manassas, VA. E. coli strains DH5α and LMG194 were from Invitrogen (Carlsbad, CA). .. E. coli strain JW1375 was from the Coli Genetic Stock Center (CGSC), New Haven, CT. Cloning was carried out in E. coli strain XL1-Blue MRF′ (Stratagene, La Jolla, CA).

Recombinant:

Article Title: Purification and Characterization of PBP4a, a New Low-Molecular-Weight Penicillin-Binding Protein from Bacillus subtilis
Article Snippet: Paragraph title: Recombinant DNA techniques, bacterial strains, plasmids, and growth conditions. ... The cloning and expression experiments were performed with E. coli TG1 (Amersham Pharmacia Biotech, Roosendaal, The Netherlands), JM109, JM110 (Stratagene, Amsterdam Zuidoost, The Netherlands), Top 10, and LMG194 (Invitrogen, Groningen, The Netherlands) using pMK4 , a shuttle Bacillus-E. coli plasmid, or the pBAD/Myc-HisA (Invitrogen) vectors.

Agarose Gel Electrophoresis:

Article Title: Purification and Characterization of PBP4a, a New Low-Molecular-Weight Penicillin-Binding Protein from Bacillus subtilis
Article Snippet: The cloning and expression experiments were performed with E. coli TG1 (Amersham Pharmacia Biotech, Roosendaal, The Netherlands), JM109, JM110 (Stratagene, Amsterdam Zuidoost, The Netherlands), Top 10, and LMG194 (Invitrogen, Groningen, The Netherlands) using pMK4 , a shuttle Bacillus-E. coli plasmid, or the pBAD/Myc-HisA (Invitrogen) vectors. .. The 3.1-kb fragment was purified from an agarose gel with the help of the Geneclean spin kit (Bio 101, Vista, Calif.), blunt-ended by filling with the Klenow DNA polymerase, and ligated to the 1.3-kb kanamycin resistance fragment isolated from the pYZ4 plasmid ( ) by an Nla IV digestion, followed by the filling of the recessed 3′ ends.

In Vitro:

Article Title: A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales
Article Snippet: Paragraph title: Protein characterization in vitro ... LMG194 or TOP10 host cells (Invitrogen) were used for protein expression.

Acetylene Reduction Assay:

Article Title: Vectors for ligation-independent construction of lacZ gene fusions and cloning of PCR products using a nicking endonuclease
Article Snippet: .. Promoters were cloned from E. coli K-12 strains, including LMG194 (F− mcrA , Δ[ mrr - hsdRMS-mcrBC ] Φ80 lacZ ΔM15, Δ lacX74 , recA1 , araD139 , Δ( ara-leu )7697, galU , galK , rpsL , endA1 , nupG ) (obtained from Invitrogen, Inc., Carlsbad, CA), or derivatives of Y. pestis strain CO92 ( ) deleted for either the pgm locus ( pgm ) ( ) or pCD1, the large virulence plasmid required for pathogenesis (Lcr− ) , enabling manipulation under BSL-2 laboratory conditions. .. E. coli NEB 5-alpha ( fhuA2 Δ (argF-lacZ)U169 phoA glnV44 Φ 80 Δ (lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 ) was obtained from New England Biolabs (NEB) (Ipswich, MA) and used as a host for all cloning experiments.

Mutagenesis:

Article Title: Bcl-2-mediated alterations in endoplasmic reticulum Ca2+ analyzed with an improved genetically encoded fluorescent sensor
Article Snippet: The mutant peptide and calmodulins (CaMs) were cloned between a truncated enhanced cyan fluorescent protein (CFP) and citrine fluorescent protein , as described ( ). .. For protein purification, cameleons were expressed in either JM109 (Stratagene) or LMG194 (Invitrogen) and grown over-night at 25°C.

Isolation:

Article Title: Purification and Characterization of PBP4a, a New Low-Molecular-Weight Penicillin-Binding Protein from Bacillus subtilis
Article Snippet: The cloning and expression experiments were performed with E. coli TG1 (Amersham Pharmacia Biotech, Roosendaal, The Netherlands), JM109, JM110 (Stratagene, Amsterdam Zuidoost, The Netherlands), Top 10, and LMG194 (Invitrogen, Groningen, The Netherlands) using pMK4 , a shuttle Bacillus-E. coli plasmid, or the pBAD/Myc-HisA (Invitrogen) vectors. .. The 3.1-kb fragment was purified from an agarose gel with the help of the Geneclean spin kit (Bio 101, Vista, Calif.), blunt-ended by filling with the Klenow DNA polymerase, and ligated to the 1.3-kb kanamycin resistance fragment isolated from the pYZ4 plasmid ( ) by an Nla IV digestion, followed by the filling of the recessed 3′ ends.

Article Title: Cloning, expression, and characterization of a peptidoglycan hydrolase from the Burkholderia pseudomallei phage ST79
Article Snippet: The B. pseudomallei lytic phage ST79, isolated from soil in the northeast of Thailand, was used as a source of the peptidase M15A for cloning (Yordpratum et al. ). .. Eighteen Gram-negative bacteria, five of which were E. coli host strains; Top10, LMG194 (Invitrogen, CA, USA), DH5α, BL21 (DE3) and XL1-Blue (Thermo Fisher Scientific, Waltham, MA, USA), two B. pseudomallei isolates, P37 and G1; two Burkholderia mallei isolates, EY2233 and EY2237; Burkholderia thailandensis UE5 (kindly provided by MORU, Mahidol University, Thailand), Klebsiella pneumoniae , Vibrio parahaemolyticus , Pseudomonas vasculitis , P. aeruginosa , Acinetobacter baumannii , Salmonella gr.

Protein Extraction:

Article Title: Bcl-2-mediated alterations in endoplasmic reticulum Ca2+ analyzed with an improved genetically encoded fluorescent sensor
Article Snippet: For protein purification, cameleons were expressed in either JM109 (Stratagene) or LMG194 (Invitrogen) and grown over-night at 25°C. .. Protein was extracted with Bacterial Protein Extraction Reagent (Pierce), purified via an N-terminal 6×Histag by using Ni-NTA agarose, and buffer-exchanged into 10 mM 4-morpholinepropanesulfonic acid/100 mM NaCl, pH 7.4, by using Amicon Centricon-30 columns (Millipore).

Construct:

Article Title: Bcl-2-mediated alterations in endoplasmic reticulum Ca2+ analyzed with an improved genetically encoded fluorescent sensor
Article Snippet: The constructs were cloned between the Bam HI/ Eco RI sites in pRSETB or pBAD for protein purification and pcDNA3 for expression in mammalian cells. .. For protein purification, cameleons were expressed in either JM109 (Stratagene) or LMG194 (Invitrogen) and grown over-night at 25°C.

Purification:

Article Title: Bcl-2-mediated alterations in endoplasmic reticulum Ca2+ analyzed with an improved genetically encoded fluorescent sensor
Article Snippet: For protein purification, cameleons were expressed in either JM109 (Stratagene) or LMG194 (Invitrogen) and grown over-night at 25°C. .. Protein was extracted with Bacterial Protein Extraction Reagent (Pierce), purified via an N-terminal 6×Histag by using Ni-NTA agarose, and buffer-exchanged into 10 mM 4-morpholinepropanesulfonic acid/100 mM NaCl, pH 7.4, by using Amicon Centricon-30 columns (Millipore).

Article Title: Bright monomeric near-infrared fluorescent proteins as tags and biosensors for multiscale imaging
Article Snippet: In addition to LMG194, TOP10 bacterial cells (Life Technologies/Invitrogen) bearing the pWA23h plasmid were used as a host. .. Proteins were purified with Ni-NTA agarose (Qiagen).

Article Title: Purification and Characterization of PBP4a, a New Low-Molecular-Weight Penicillin-Binding Protein from Bacillus subtilis
Article Snippet: The cloning and expression experiments were performed with E. coli TG1 (Amersham Pharmacia Biotech, Roosendaal, The Netherlands), JM109, JM110 (Stratagene, Amsterdam Zuidoost, The Netherlands), Top 10, and LMG194 (Invitrogen, Groningen, The Netherlands) using pMK4 , a shuttle Bacillus-E. coli plasmid, or the pBAD/Myc-HisA (Invitrogen) vectors. .. The 3.1-kb fragment was purified from an agarose gel with the help of the Geneclean spin kit (Bio 101, Vista, Calif.), blunt-ended by filling with the Klenow DNA polymerase, and ligated to the 1.3-kb kanamycin resistance fragment isolated from the pYZ4 plasmid ( ) by an Nla IV digestion, followed by the filling of the recessed 3′ ends.

Protein Purification:

Article Title: Bcl-2-mediated alterations in endoplasmic reticulum Ca2+ analyzed with an improved genetically encoded fluorescent sensor
Article Snippet: .. For protein purification, cameleons were expressed in either JM109 (Stratagene) or LMG194 (Invitrogen) and grown over-night at 25°C. .. Constructs in pBAD/LMG194 were induced with 0.2% arabinose.

Incubation:

Article Title: A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales
Article Snippet: LMG194 or TOP10 host cells (Invitrogen) were used for protein expression. .. Bacterial cells were incubated overnight at 37 °C in RM minimal medium with ampicillin and kanamycin.

Infection:

Article Title: Inorganic pyrophosphatase in uncultivable hemotrophic mycoplasmas: identification and properties of the enzyme from Mycoplasma suis
Article Snippet: Bacterial strains and isolates, plasmids, and experimental porcine sera M. suis cells were obtained from experimentally infected pigs as previously described [ , ]. .. E. coli K12 strains were Top10 and LMG194 (Invitrogen, Basel, Switzerland).

Cell Culture:

Article Title: Vectors for ligation-independent construction of lacZ gene fusions and cloning of PCR products using a nicking endonuclease
Article Snippet: Promoters were cloned from E. coli K-12 strains, including LMG194 (F− mcrA , Δ[ mrr - hsdRMS-mcrBC ] Φ80 lacZ ΔM15, Δ lacX74 , recA1 , araD139 , Δ( ara-leu )7697, galU , galK , rpsL , endA1 , nupG ) (obtained from Invitrogen, Inc., Carlsbad, CA), or derivatives of Y. pestis strain CO92 ( ) deleted for either the pgm locus ( pgm ) ( ) or pCD1, the large virulence plasmid required for pathogenesis (Lcr− ) , enabling manipulation under BSL-2 laboratory conditions. .. Y. pestis mutants were grown on Tryptic Soy Blood Agar Base #[0–9][a-zA-Z]2 (TSB) from Difco (obtained through Thermo Scientific, Waltham, MA), while E. coli was cultured using LB medium ( ).

Expressing:

Article Title: Inorganic pyrophosphatase in uncultivable hemotrophic mycoplasmas: identification and properties of the enzyme from Mycoplasma suis
Article Snippet: E. coli K12 strains were Top10 and LMG194 (Invitrogen, Basel, Switzerland). .. For DNA manipulation and protein expression the plasmids pUC19 (Roche-Diagnostics, Rotkreuz, Switzerland) and pBadMyc His (C-terminal His- and Myc -tag, Invitrogen) were used.

Article Title: Bcl-2-mediated alterations in endoplasmic reticulum Ca2+ analyzed with an improved genetically encoded fluorescent sensor
Article Snippet: The constructs were cloned between the Bam HI/ Eco RI sites in pRSETB or pBAD for protein purification and pcDNA3 for expression in mammalian cells. .. For protein purification, cameleons were expressed in either JM109 (Stratagene) or LMG194 (Invitrogen) and grown over-night at 25°C.

Article Title: Bright monomeric near-infrared fluorescent proteins as tags and biosensors for multiscale imaging
Article Snippet: Paragraph title: Protein expression and characterization ... In addition to LMG194, TOP10 bacterial cells (Life Technologies/Invitrogen) bearing the pWA23h plasmid were used as a host.

Article Title: Purification and Characterization of PBP4a, a New Low-Molecular-Weight Penicillin-Binding Protein from Bacillus subtilis
Article Snippet: .. The cloning and expression experiments were performed with E. coli TG1 (Amersham Pharmacia Biotech, Roosendaal, The Netherlands), JM109, JM110 (Stratagene, Amsterdam Zuidoost, The Netherlands), Top 10, and LMG194 (Invitrogen, Groningen, The Netherlands) using pMK4 , a shuttle Bacillus-E. coli plasmid, or the pBAD/Myc-HisA (Invitrogen) vectors. .. The ampicillin resistance gene of the last plasmid was replaced by the kanamycin resistance gene using the following strategy.

Article Title: A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales
Article Snippet: .. LMG194 or TOP10 host cells (Invitrogen) were used for protein expression. .. A pWA23h plasmid encoding HO from Bradyrhizobium ORS278 (hmuO) under the rhamnose promoter was co-transformed with a pBAD/His-B plasmid encoding a FP.

Article Title: Cloning, expression, and characterization of a peptidoglycan hydrolase from the Burkholderia pseudomallei phage ST79
Article Snippet: Escherichia coli BL21 (DE3) was used as the host for cloning and protein expression processes (Thermo Fisher Scientific, Waltham, MA, USA). .. Eighteen Gram-negative bacteria, five of which were E. coli host strains; Top10, LMG194 (Invitrogen, CA, USA), DH5α, BL21 (DE3) and XL1-Blue (Thermo Fisher Scientific, Waltham, MA, USA), two B. pseudomallei isolates, P37 and G1; two Burkholderia mallei isolates, EY2233 and EY2237; Burkholderia thailandensis UE5 (kindly provided by MORU, Mahidol University, Thailand), Klebsiella pneumoniae , Vibrio parahaemolyticus , Pseudomonas vasculitis , P. aeruginosa , Acinetobacter baumannii , Salmonella gr.

Sequencing:

Article Title: Bcl-2-mediated alterations in endoplasmic reticulum Ca2+ analyzed with an improved genetically encoded fluorescent sensor
Article Snippet: To generate an ER-targeted cameleon, the calreticulin signal sequence MLLPVLLLGLLGAAAD was added 5′ to CFP, and an ER retention sequence, KDEL, was added to the 3′ end of citrine. .. For protein purification, cameleons were expressed in either JM109 (Stratagene) or LMG194 (Invitrogen) and grown over-night at 25°C.

Over Expression:

Article Title: Functional Domain Analysis of the Cell Division Inhibitor EzrA
Article Snippet: .. We used E. coli strain AG1111 and TOP10 (Invitrogen) for plasmid construction, strain BB101 and LMG194 (Invitrogen) for protein overexpression. .. Vent DNA polymerase (NEB) was used in PCRs.

Chick Chorioallantoic Membrane Assay:

Article Title: Vectors for ligation-independent construction of lacZ gene fusions and cloning of PCR products using a nicking endonuclease
Article Snippet: Promoters were cloned from E. coli K-12 strains, including LMG194 (F− mcrA , Δ[ mrr - hsdRMS-mcrBC ] Φ80 lacZ ΔM15, Δ lacX74 , recA1 , araD139 , Δ( ara-leu )7697, galU , galK , rpsL , endA1 , nupG ) (obtained from Invitrogen, Inc., Carlsbad, CA), or derivatives of Y. pestis strain CO92 ( ) deleted for either the pgm locus ( pgm ) ( ) or pCD1, the large virulence plasmid required for pathogenesis (Lcr− ) , enabling manipulation under BSL-2 laboratory conditions. .. When appropriate, media were supplemented with antibiotics including ampicillin (Amp, 100 g/ml), chloramphenicol (Cam, 20 g/ml), or kanamycin (Kan, 30 g/ml) (Sigma-Aldrich, St. Louis, MO).

Plasmid Preparation:

Article Title: Bright monomeric near-infrared fluorescent proteins as tags and biosensors for multiscale imaging
Article Snippet: .. In addition to LMG194, TOP10 bacterial cells (Life Technologies/Invitrogen) bearing the pWA23h plasmid were used as a host. .. Proteins were purified with Ni-NTA agarose (Qiagen).

Article Title: Purification and Characterization of PBP4a, a New Low-Molecular-Weight Penicillin-Binding Protein from Bacillus subtilis
Article Snippet: .. The cloning and expression experiments were performed with E. coli TG1 (Amersham Pharmacia Biotech, Roosendaal, The Netherlands), JM109, JM110 (Stratagene, Amsterdam Zuidoost, The Netherlands), Top 10, and LMG194 (Invitrogen, Groningen, The Netherlands) using pMK4 , a shuttle Bacillus-E. coli plasmid, or the pBAD/Myc-HisA (Invitrogen) vectors. .. The ampicillin resistance gene of the last plasmid was replaced by the kanamycin resistance gene using the following strategy.

Article Title: A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales
Article Snippet: Protein characterization in vitro For expression in bacteria, the miRFPs were cloned into pBAD/His-B vector (Life Technologies/Invitrogen). .. LMG194 or TOP10 host cells (Invitrogen) were used for protein expression.

Article Title: Vectors for ligation-independent construction of lacZ gene fusions and cloning of PCR products using a nicking endonuclease
Article Snippet: .. Promoters were cloned from E. coli K-12 strains, including LMG194 (F− mcrA , Δ[ mrr - hsdRMS-mcrBC ] Φ80 lacZ ΔM15, Δ lacX74 , recA1 , araD139 , Δ( ara-leu )7697, galU , galK , rpsL , endA1 , nupG ) (obtained from Invitrogen, Inc., Carlsbad, CA), or derivatives of Y. pestis strain CO92 ( ) deleted for either the pgm locus ( pgm ) ( ) or pCD1, the large virulence plasmid required for pathogenesis (Lcr− ) , enabling manipulation under BSL-2 laboratory conditions. .. E. coli NEB 5-alpha ( fhuA2 Δ (argF-lacZ)U169 phoA glnV44 Φ 80 Δ (lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 ) was obtained from New England Biolabs (NEB) (Ipswich, MA) and used as a host for all cloning experiments.

Article Title: Functional Domain Analysis of the Cell Division Inhibitor EzrA
Article Snippet: .. We used E. coli strain AG1111 and TOP10 (Invitrogen) for plasmid construction, strain BB101 and LMG194 (Invitrogen) for protein overexpression. .. Vent DNA polymerase (NEB) was used in PCRs.

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    Thermo Fisher bacteria preparation mirfp670 expressing lmg194
    Bacteria Preparation Mirfp670 Expressing Lmg194, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bacteria preparation mirfp670 expressing lmg194/product/Thermo Fisher
    Average 83 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bacteria preparation mirfp670 expressing lmg194 - by Bioz Stars, 2020-02
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    93
    Thermo Fisher lmg194 e coli cells
    Lmg194 E Coli Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lmg194 e coli cells/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lmg194 e coli cells - by Bioz Stars, 2020-02
    93/100 stars
      Buy from Supplier

    83
    Thermo Fisher mirfp670 expressing lmg194
    Mirfp670 Expressing Lmg194, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mirfp670 expressing lmg194/product/Thermo Fisher
    Average 83 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mirfp670 expressing lmg194 - by Bioz Stars, 2020-02
    83/100 stars
      Buy from Supplier

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