liquid chromatography mass spectrometry lc ms grade formic acid  (Thermo Fisher)


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    Name:
    Pierce Formic Acid LC MS Grade
    Description:
    Thermo Scientific Pierce Formic Acid is high purity solvent supplied in bottles or ampules as a convenient contamination free alternative for preparing elution solvents for HPLC separations of protein and peptides This product is packaged in amber glass pre scored nitrogen flushed ampules to protect formic acid from light moisture and contamination Features of Formic Acid • Greater than 99 pure formic acid consistent LC baselines no potential interference introduced in LC or MS applications and no signal suppression in the mass spectrometer • Convenient format ampule packaging simplifies the preparation of gradient and isocratic mobile phases containing 0 1 v v formic acid in water or acetonitrile the contents of a single vial in a final volume of 1 L of solvent yields a mobile phase of the most common formic acid concentration General properties of formic acid • Molecular formula HCOOH CH2O2 • Molecular weight 46 02 • Density 1 22 g mL • CAS number 64 18 6 • Refractive index 1 3701 1 3721 20°C • Flash point 69°C • Freezing point 8°C Specifications of Thermo Scientific Pierce Formic Acid • Visual Clear liquid free of particulate matter • Identity IR Must show only peaks characteristic for the compound • Purity 99 • Refractive index 1 3701 to 1 3721 20°C 589 nm Pierce Formic Acid is sealed in amber glass ampules under a dry nitrogen atmosphere A pre measured aliquot of acid greatly simplifies preparation of liter quantities of mobile phases at the standard 0 1 formic acid concentration The quality of this formic acid coupled with either glass ampule bottle packaging provides reliability and convenience that adds value to both the chromatographic and MS results Pierce Formic Acid is specially purified and tested to the highest specifications to ensure the integrity of your data maximize sensitivity in your assay and to prolong the life of your equipment Formic acid is a common component of reverse phase mobile phases that provide protons for LC MS analysis The presence of a low concentration of formic acid in the mobile phase is also known to improve the peak shapes of the resulting separation Unlike trifluoroacetic acid TFA formic acid is not an ion pairing agent and it does not suppress MS ionization of polypeptides when used as a mobile phase component For added safety when opening glass ampules consider using our Ampule Breakers which are disposable safety devices that protect the fingers when breaking open a glass ampule
    Catalog Number:
    28905
    Price:
    None
    Applications:
    Calibration Solutions, Standards and Solvents for Mass Spectrometry|Protein Biology|Protein Mass Spectrometry Analysis
    Category:
    Lab Reagents and Chemicals
    Buy from Supplier


    Structured Review

    Thermo Fisher liquid chromatography mass spectrometry lc ms grade formic acid
    Thermo Scientific Pierce Formic Acid is high purity solvent supplied in bottles or ampules as a convenient contamination free alternative for preparing elution solvents for HPLC separations of protein and peptides This product is packaged in amber glass pre scored nitrogen flushed ampules to protect formic acid from light moisture and contamination Features of Formic Acid • Greater than 99 pure formic acid consistent LC baselines no potential interference introduced in LC or MS applications and no signal suppression in the mass spectrometer • Convenient format ampule packaging simplifies the preparation of gradient and isocratic mobile phases containing 0 1 v v formic acid in water or acetonitrile the contents of a single vial in a final volume of 1 L of solvent yields a mobile phase of the most common formic acid concentration General properties of formic acid • Molecular formula HCOOH CH2O2 • Molecular weight 46 02 • Density 1 22 g mL • CAS number 64 18 6 • Refractive index 1 3701 1 3721 20°C • Flash point 69°C • Freezing point 8°C Specifications of Thermo Scientific Pierce Formic Acid • Visual Clear liquid free of particulate matter • Identity IR Must show only peaks characteristic for the compound • Purity 99 • Refractive index 1 3701 to 1 3721 20°C 589 nm Pierce Formic Acid is sealed in amber glass ampules under a dry nitrogen atmosphere A pre measured aliquot of acid greatly simplifies preparation of liter quantities of mobile phases at the standard 0 1 formic acid concentration The quality of this formic acid coupled with either glass ampule bottle packaging provides reliability and convenience that adds value to both the chromatographic and MS results Pierce Formic Acid is specially purified and tested to the highest specifications to ensure the integrity of your data maximize sensitivity in your assay and to prolong the life of your equipment Formic acid is a common component of reverse phase mobile phases that provide protons for LC MS analysis The presence of a low concentration of formic acid in the mobile phase is also known to improve the peak shapes of the resulting separation Unlike trifluoroacetic acid TFA formic acid is not an ion pairing agent and it does not suppress MS ionization of polypeptides when used as a mobile phase component For added safety when opening glass ampules consider using our Ampule Breakers which are disposable safety devices that protect the fingers when breaking open a glass ampule
    https://www.bioz.com/result/liquid chromatography mass spectrometry lc ms grade formic acid/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    liquid chromatography mass spectrometry lc ms grade formic acid - by Bioz Stars, 2020-12
    99/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Subcellular localization of the J-protein Sis1 regulates the heat shock response
    Article Snippet: .. HPLC for mass spectrometry All samples were resuspended in Burdick & Jackson HPLC-grade water containing 0.2% formic acid (Fluka), 0.1% TFA (Pierce), and 0.002% Zwittergent 3-16 (Calbiochem), a sulfobetaine detergent that contributes the following distinct peaks at the end of chromatograms: MH+ at 392, and in-source dimer [2M + H+] at 783, and some minor impurities of Zwittergent 3-12 seen as MH+ at 336. .. The peptide samples were loaded to a 0.25 μl C8 OptiPak trapping cartridge custom-packed with Michrom Magic (Optimize Technologies) C8, washed, then switched in-line with a 20 cm by 75 μm C18 packed spray tip nano column packed with Michrom Magic C18AQ, for a 2-step gradient.

    Bicinchoninic Acid Protein Assay:

    Article Title: Effect of iron oxide nanoparticles on the oxidation and secondary structure of growth hormone
    Article Snippet: .. Ammonium bicarbonate, dithiothreitol (DTT), iodoacetamide (IAA), LC/MS-grade water (0.1% v/v formic acid), acetonitrile (0.1% v/v formic acid), monobasic sodium dihydrogen phosphate, sulfuric acid, hydrogen fluoride, dibasic sodium hydrogen phosphate, and the Pierce BCA protein assay kit (Product number: 23227) were purchased from Fisher Scientific (Waltham, MA). .. Trypsin/Lys-C (mass spectrometry grade) was purchased from Promega (Madison, Wl).

    Mass Spectrometry:

    Article Title: Subcellular localization of the J-protein Sis1 regulates the heat shock response
    Article Snippet: .. HPLC for mass spectrometry All samples were resuspended in Burdick & Jackson HPLC-grade water containing 0.2% formic acid (Fluka), 0.1% TFA (Pierce), and 0.002% Zwittergent 3-16 (Calbiochem), a sulfobetaine detergent that contributes the following distinct peaks at the end of chromatograms: MH+ at 392, and in-source dimer [2M + H+] at 783, and some minor impurities of Zwittergent 3-12 seen as MH+ at 336. .. The peptide samples were loaded to a 0.25 μl C8 OptiPak trapping cartridge custom-packed with Michrom Magic (Optimize Technologies) C8, washed, then switched in-line with a 20 cm by 75 μm C18 packed spray tip nano column packed with Michrom Magic C18AQ, for a 2-step gradient.

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Impact of Tryptophan Oxidation in Complementarity-Determining Regions of Two Monoclonal Antibodies on Structure-Function Characterized by Hydrogen-Deuterium Exchange Mass Spectrometry and Surface Plasmon Resonance
    Article Snippet: .. 8 M Guanidine-HCl, premium grade TCEP-HCl, and LC-MS grade formic acid were purchased from Thermo Scientific Pierce (Grand Island, NY). ..

    Article Title: Enrichment of plasma membrane proteins using nanoparticle pellicles: comparison between silica and higher density nanoparticles
    Article Snippet: .. Optima LC/MS grade acetonitrile, formic acid, trifluoroacetic acid, Pierce® C18 spin columns were purchased from Fisher Scientific (Pittsburgh PA). .. Pierce® C18 spin columns were purchased from Fisher Scientific.

    Article Title: Synthesis of site-specific antibody-drug conjugates by ADP-ribosyl cyclases
    Article Snippet: .. The 5-min method used a flow rate (0.2 ml/min) of a gradient of buffer A consisting of 0.1% formic acid in water (water LC-MS #9831-02, JT Baker; formic acid LC-MS #85178, Thermo Fisher Scientific) and buffer B, acetonitrile (acetonitrile UHPLC/MS #A956, Thermo Fisher Scientific). ..

    Article Title: RawTools: Rapid and Dynamic Interrogation of Orbitrap Data Files for Mass Spectrometer System Management
    Article Snippet: .. Eluted peptides were concentrated in a SpeedVac centrifuge (Thermo Scientific) and subsequently reconstituted in 1% formic acid (Thermo Scientific, LC-MS grade, CAT#85178) with 1% dimethylsulfoxide (Sigma, CAT#D4540) in water. .. Mass spectrometry data acquisition Analysis of peptides was carried out on an Orbitrap Velos MS platform (Thermo Scientific).

    Article Title: RIPK2 Stabilizes c-Myc and is an Actionable Target for Inhibiting Prostate Cancer Metastasis
    Article Snippet: .. TRIzol Reagent (#15596026), 660nm Protein Assay Reagent (#22660), SuperSignal West Pico PLUS (#34577) and Femto (#34096) chemiluminescent substrates, dithiothreitol (DTT) (#20290), MS-grade trypsin (90059), LC-MS grade formic acid (#85178), 2-cm Acclaim PepMap 100 trap columns (#164564), and 50-cm EASY-Spray columns (ES803) were from Thermo Scientific. .. Autoradiography films were from Thomas Scientific.

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  • 99
    Thermo Fisher formic acid
    Formic Acid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/formic acid/product/Thermo Fisher
    Average 99 stars, based on 19 article reviews
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    Thermo Fisher solvent b
    Solvent B, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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