liquid chromatography mass spectrometry lc ms grade acetonitrile acn  (Millipore)


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    Liquid Chromatography
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    With the advent of new interfacing technology the benefits of LC MS liquid chromatography with mass spectrometry are being realized by the growth in applications of this technique in both the chemical and life sciences Topics covered include Limitations of the component techniques when used in isolation and how a combination of the two allows these to be overcome Descriptions of the various approaches including thermospray electrospray and atmospheric pressure chemical ionization which are used to interface the two techniques along with the advantages and disadvantages of each system Illustrative examples of the applications of LC MS including the molecular weight and structure determinations of both biopolymers and low molecular compounds
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    z702080
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    Millipore liquid chromatography mass spectrometry lc ms grade acetonitrile acn
    Liquid Chromatography
    With the advent of new interfacing technology the benefits of LC MS liquid chromatography with mass spectrometry are being realized by the growth in applications of this technique in both the chemical and life sciences Topics covered include Limitations of the component techniques when used in isolation and how a combination of the two allows these to be overcome Descriptions of the various approaches including thermospray electrospray and atmospheric pressure chemical ionization which are used to interface the two techniques along with the advantages and disadvantages of each system Illustrative examples of the applications of LC MS including the molecular weight and structure determinations of both biopolymers and low molecular compounds
    https://www.bioz.com/result/liquid chromatography mass spectrometry lc ms grade acetonitrile acn/product/Millipore
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    High Performance Liquid Chromatography:

    Article Title: Time-series lipidomic analysis of the oleaginous green microalga species Ettlia oleoabundans under nutrient stress
    Article Snippet: .. Liquid chromatography - mass spectrometry (LC–MS) grade methanol and high-performance liquid chromatography (HPLC) grade isopropanol were purchased from Millipore Sigma (Billerica, MA). .. Formic acid, ammonium formate, and ammonium hydroxide were purchased from Sigma-Aldrich (St. Louis, MO).

    Article Title: Biosynthesis of Diterpenoids in Tripterygium Adventitious Root Cultures 1 Adventitious Root Cultures 1 [OPEN]
    Article Snippet: .. Acetone was of HPLC grade (Fisher Scientific), ethyl acetate was ACS quality (Avantor Performance Materials), ethanol was Omnisolv (EMD Serono), and acetonitrile and water were liquid chromatography-mass spectrometry grade (Sigma-Aldrich). .. CDCl3 was obtained from Cambridge Isotope Laboratories.

    Indirect Immunoperoxidase Assay:

    Article Title: Neonatal Screening for Congenital Adrenal Hyperplasia in Turkey: A Pilot Study with 38,935 Infants
    Article Snippet: Reagent, Instruments, and Analytical Conditions for Liquid Chromatography-tandem Mass Spectrometry The steroid standards for F, 17-OHP, 21-S, 11-S, 4AS and deuterated steroid standards for d4-F, d8-17-OHP, d3-testosterone were purchased from Sigma-Aldrich (MO, USA). .. Acetonitrile (ACN), methanol, ethanol, isopropyl alcohol (IPA), formic acid and LC-MS grade water were purchased from Merck (Darmstadt, Germany).

    Mass Spectrometry:

    Article Title: Neonatal Screening for Congenital Adrenal Hyperplasia in Turkey: A Pilot Study with 38,935 Infants
    Article Snippet: .. Reagent, Instruments, and Analytical Conditions for Liquid Chromatography-tandem Mass Spectrometry The steroid standards for F, 17-OHP, 21-S, 11-S, 4AS and deuterated steroid standards for d4-F, d8-17-OHP, d3-testosterone were purchased from Sigma-Aldrich (MO, USA). .. Acetonitrile (ACN), methanol, ethanol, isopropyl alcohol (IPA), formic acid and LC-MS grade water were purchased from Merck (Darmstadt, Germany).

    Article Title: Dietary Leucine Supplement Ameliorates Hepatic Steatosis and Diabetic Nephropathy in db/db Mice
    Article Snippet: .. Liquid Chromatography/Time-of-Flight Mass Spectrometry (LC/TOF MS)–Based Metabolomics Analysis of Urine Samples Urine was normalized with water to contain a creatinine concentration of 50 ug/mL and was filtered with 0.2 μm filter (millipore) to remove particles. .. The clear diluent was analyzed using LC/TOFMS (Waters Corp.).

    Article Title: Multi-modal mass spectrometric imaging of small molecules reveals distinct spatio-molecular signatures in differentially metastatic breast tumor models
    Article Snippet: After the sections reached room temperature, different sample preparation procedures were applied depending on the MS imaging techniques used in this study. .. For Matrix Enhanced (ME)-SIMS and MALDI-MS, the tissues were covered with 2,5-Dihydroxybenzoic acid (DHB) 30mg/mL in 50% acetonitrile/0.1% aqueous trifluoroacetic acid using air driven thin liquid chromatography sprayer (Sigma).

    Article Title: Response of Porphyromonas gingivalis to Heme Limitation in Continuous Culture ▿ to Heme Limitation in Continuous Culture ▿ †
    Article Snippet: .. Chemicals used were ultra-high purity, except for MS work, where liquid chromatography (LC)-MS-grade reagents were used (Sigma). .. P. gingivalis W50 was grown in continuous culture in a Bioflo 110 fermentor/bioreactor (New Brunswick Scientific) with a 400-ml working volume.

    Article Title: Time-series lipidomic analysis of the oleaginous green microalga species Ettlia oleoabundans under nutrient stress
    Article Snippet: .. Liquid chromatography - mass spectrometry (LC–MS) grade methanol and high-performance liquid chromatography (HPLC) grade isopropanol were purchased from Millipore Sigma (Billerica, MA). .. Formic acid, ammonium formate, and ammonium hydroxide were purchased from Sigma-Aldrich (St. Louis, MO).

    Article Title: Real-time analysis of the binding of fluorescent VEGF165a to VEGFR2 in living cells: Effect of receptor tyrosine kinase inhibitors and fate of internalized agonist-receptor complexes
    Article Snippet: .. 2.8 Liquid chromatography–tandem mass spectrometry (LC–MS/MS) analysis VEGF165 a-TMR and VEGF165 a purified in the same manner were suspended in 50 mM ammonium bicarbonate (0.1 mg/ml; Sigma-Aldrich, USA) and reduced with 25 mM TCEP at 50 °C for 1 h. Following reduction, the appropriate protease (LysC, GluC, or trypsin/LysC from Promega Corporation, USA) were added to the sample (1:20 enzyme/substrate ratio) and subjected to proteolysis for 3 h at 37 °C with shaking at 400 rpm (Eppendorf Thermomixer). .. Following this step, the reaction was quenched by the addition of formic acid (1% final concentration; Sigma-Aldrich, USA) and subjected to LC-MS/MS analysis on a Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Fisher Scientific, USA) operating with an MS resolution of 70,000.

    Article Title: Inactivation of Chloramphenicol and Florfenicol by a Novel Chloramphenicol Hydrolase
    Article Snippet: .. The Cm hydrolysate isolated was subjected to liquid chromatography (LC)-MS analysis, and the MS of the Cm hydrolysate was apparently identical to that of p -nitrophenylserinol (Sigma-Aldrich, St. Louis, MO), which was at m/z 213.2 [M+H]+ ; its dehydrated product was at m/z 195.2 [M+H-H2 O]+ ( ). .. In addition, 1 H-NMR analysis demonstrated that the chemical shift of the Cm hydrolysate and p -nitrophenylserinol peaks were identical ( and ).

    Sample Prep:

    Article Title: Urinary Metabolite Profiling Combined with Computational Analysis Predicts Interstitial Cystitis-Associated Candidate Biomarkers
    Article Snippet: .. All reagents for sample preparation for NMR and liquid chromatography–mass spectrometry (LC–MS) analysis as well as tyramine and 2-oxoglutarate were obtained from Sigma-Aldrich. ..

    Article Title: Multi-modal mass spectrometric imaging of small molecules reveals distinct spatio-molecular signatures in differentially metastatic breast tumor models
    Article Snippet: Paragraph title: Sample preparation ... For Matrix Enhanced (ME)-SIMS and MALDI-MS, the tissues were covered with 2,5-Dihydroxybenzoic acid (DHB) 30mg/mL in 50% acetonitrile/0.1% aqueous trifluoroacetic acid using air driven thin liquid chromatography sprayer (Sigma).

    Proton NMR:

    Article Title: Inactivation of Chloramphenicol and Florfenicol by a Novel Chloramphenicol Hydrolase
    Article Snippet: The Cm hydrolysate isolated was subjected to liquid chromatography (LC)-MS analysis, and the MS of the Cm hydrolysate was apparently identical to that of p -nitrophenylserinol (Sigma-Aldrich, St. Louis, MO), which was at m/z 213.2 [M+H]+ ; its dehydrated product was at m/z 195.2 [M+H-H2 O]+ ( ). .. In addition, 1 H-NMR analysis demonstrated that the chemical shift of the Cm hydrolysate and p -nitrophenylserinol peaks were identical ( and ).

    Chromatography:

    Article Title: Urinary Metabolite Profiling Combined with Computational Analysis Predicts Interstitial Cystitis-Associated Candidate Biomarkers
    Article Snippet: .. All reagents for sample preparation for NMR and liquid chromatography–mass spectrometry (LC–MS) analysis as well as tyramine and 2-oxoglutarate were obtained from Sigma-Aldrich. ..

    Article Title: Neonatal Screening for Congenital Adrenal Hyperplasia in Turkey: A Pilot Study with 38,935 Infants
    Article Snippet: .. Reagent, Instruments, and Analytical Conditions for Liquid Chromatography-tandem Mass Spectrometry The steroid standards for F, 17-OHP, 21-S, 11-S, 4AS and deuterated steroid standards for d4-F, d8-17-OHP, d3-testosterone were purchased from Sigma-Aldrich (MO, USA). .. Acetonitrile (ACN), methanol, ethanol, isopropyl alcohol (IPA), formic acid and LC-MS grade water were purchased from Merck (Darmstadt, Germany).

    Article Title: Dietary Leucine Supplement Ameliorates Hepatic Steatosis and Diabetic Nephropathy in db/db Mice
    Article Snippet: .. Liquid Chromatography/Time-of-Flight Mass Spectrometry (LC/TOF MS)–Based Metabolomics Analysis of Urine Samples Urine was normalized with water to contain a creatinine concentration of 50 ug/mL and was filtered with 0.2 μm filter (millipore) to remove particles. .. The clear diluent was analyzed using LC/TOFMS (Waters Corp.).

    Article Title: Biosynthetically-Intriguing Chlorinated Lipophilic Metabolites from Geographically Distant Tropical Marine Cyanobacteria
    Article Snippet: A second NP-SPE cartridge chromatography, employing a stepwise gradient of 100% hexanes to 100% EtOAc, a fraction (D1F) eluting with 5% EtOAc in hexanes gave compound 1 (34.2 mg, 1.5% of extract). .. Based on preliminary 1 H NMR and LCMS analyses indicating that the extracts were virtually identical, the crude extracts were combined and fractionated using vacuum liquid chromatography (VLC) on Silica gel (Type H, 10–40 µM, Sigma-Aldrich) with a stepwise gradient of hexanes/EtOAc and EtOAc/MeOH to give nine fractions (A–I).

    Article Title: Transfer and Metabolism of the Xenoestrogen Zearalenone in Human Perfused Placenta
    Article Snippet: .. Chemicals and Reagents Liquid chromatography–mass spectrometry (LC-MS)-grade acetonitrile (ACN) and methanol (MeOH) were obtained from Sigma-Aldrich, and water ( H 2 O , LC-MS grade) was purchased from VWR International GmbH. .. Ammonium fluoride (LC-MS grade) was obtained from Honeywell Fluka.

    Article Title: Real-time analysis of the binding of fluorescent VEGF165a to VEGFR2 in living cells: Effect of receptor tyrosine kinase inhibitors and fate of internalized agonist-receptor complexes
    Article Snippet: .. 2.8 Liquid chromatography–tandem mass spectrometry (LC–MS/MS) analysis VEGF165 a-TMR and VEGF165 a purified in the same manner were suspended in 50 mM ammonium bicarbonate (0.1 mg/ml; Sigma-Aldrich, USA) and reduced with 25 mM TCEP at 50 °C for 1 h. Following reduction, the appropriate protease (LysC, GluC, or trypsin/LysC from Promega Corporation, USA) were added to the sample (1:20 enzyme/substrate ratio) and subjected to proteolysis for 3 h at 37 °C with shaking at 400 rpm (Eppendorf Thermomixer). .. Following this step, the reaction was quenched by the addition of formic acid (1% final concentration; Sigma-Aldrich, USA) and subjected to LC-MS/MS analysis on a Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Fisher Scientific, USA) operating with an MS resolution of 70,000.

    Article Title: Biosynthesis of Diterpenoids in Tripterygium Adventitious Root Cultures 1 Adventitious Root Cultures 1 [OPEN]
    Article Snippet: .. Acetone was of HPLC grade (Fisher Scientific), ethyl acetate was ACS quality (Avantor Performance Materials), ethanol was Omnisolv (EMD Serono), and acetonitrile and water were liquid chromatography-mass spectrometry grade (Sigma-Aldrich). .. CDCl3 was obtained from Cambridge Isotope Laboratories.

    Article Title: Surface Enhanced Raman Spectroscopy for In-Field Detection of Pesticides: A Test on Dimethoate Residues in Water and on Olive Leaves
    Article Snippet: .. Water used for preparation of the different solutions was LC-MS chromatography grade, obtained from Merck/Sigma-Aldrich. ..

    Column Chromatography:

    Article Title: Biosynthetically-Intriguing Chlorinated Lipophilic Metabolites from Geographically Distant Tropical Marine Cyanobacteria
    Article Snippet: Based on preliminary 1 H NMR and LCMS analyses indicating that the extracts were virtually identical, the crude extracts were combined and fractionated using vacuum liquid chromatography (VLC) on Silica gel (Type H, 10–40 µM, Sigma-Aldrich) with a stepwise gradient of hexanes/EtOAc and EtOAc/MeOH to give nine fractions (A–I). .. In the first round of NP-SPE column chromatography, the majority of the material (127 mg) eluted under the loading condition of 30% EtOAc in hexanes.

    Isolation:

    Article Title: Biosynthetically-Intriguing Chlorinated Lipophilic Metabolites from Geographically Distant Tropical Marine Cyanobacteria
    Article Snippet: Paragraph title: Extraction and Isolation ... Based on preliminary 1 H NMR and LCMS analyses indicating that the extracts were virtually identical, the crude extracts were combined and fractionated using vacuum liquid chromatography (VLC) on Silica gel (Type H, 10–40 µM, Sigma-Aldrich) with a stepwise gradient of hexanes/EtOAc and EtOAc/MeOH to give nine fractions (A–I).

    Article Title: Inactivation of Chloramphenicol and Florfenicol by a Novel Chloramphenicol Hydrolase
    Article Snippet: .. The Cm hydrolysate isolated was subjected to liquid chromatography (LC)-MS analysis, and the MS of the Cm hydrolysate was apparently identical to that of p -nitrophenylserinol (Sigma-Aldrich, St. Louis, MO), which was at m/z 213.2 [M+H]+ ; its dehydrated product was at m/z 195.2 [M+H-H2 O]+ ( ). .. In addition, 1 H-NMR analysis demonstrated that the chemical shift of the Cm hydrolysate and p -nitrophenylserinol peaks were identical ( and ).

    Synthesized:

    Article Title: Transfer and Metabolism of the Xenoestrogen Zearalenone in Human Perfused Placenta
    Article Snippet: Chemicals and Reagents Liquid chromatography–mass spectrometry (LC-MS)-grade acetonitrile (ACN) and methanol (MeOH) were obtained from Sigma-Aldrich, and water ( H 2 O , LC-MS grade) was purchased from VWR International GmbH. .. ZEN, α -ZEL , β -ZEL , α -ZAL , β -ZAL , and C 18 13 -ZEN were obtained from either Romerlabs or Sigma-Aldrich, whereas ZEN-14-GlcA, α -ZEL-GlcA , and β -ZEL-GlcA were synthesized at the Technical University of Vienna and kindly provided by Dr. Mikula ( , ).

    Article Title: Biosynthesis of Diterpenoids in Tripterygium Adventitious Root Cultures 1 Adventitious Root Cultures 1 [OPEN]
    Article Snippet: Dehydroabietic acid was synthesized according to a literature protocol ( ). .. Acetone was of HPLC grade (Fisher Scientific), ethyl acetate was ACS quality (Avantor Performance Materials), ethanol was Omnisolv (EMD Serono), and acetonitrile and water were liquid chromatography-mass spectrometry grade (Sigma-Aldrich).

    Incubation:

    Article Title: Multi-modal mass spectrometric imaging of small molecules reveals distinct spatio-molecular signatures in differentially metastatic breast tumor models
    Article Snippet: For Matrix Enhanced (ME)-SIMS and MALDI-MS, the tissues were covered with 2,5-Dihydroxybenzoic acid (DHB) 30mg/mL in 50% acetonitrile/0.1% aqueous trifluoroacetic acid using air driven thin liquid chromatography sprayer (Sigma). .. The parameters for each cycle were 1.5 s spray time, 0.3 s incubation time and 0.7 s drying time.

    Article Title: Inactivation of Chloramphenicol and Florfenicol by a Novel Chloramphenicol Hydrolase
    Article Snippet: Large volumes of Cm (50 mg) were incubated with EstDL136, and the Cm hydrolysate (a newly produced compound detected on TLC; Rf = 0.23) was detected mainly in the butanol extract of the reaction mixture, wherein 14.2 mg of the hydrolysate was isolated further by preparative TLC. .. The Cm hydrolysate isolated was subjected to liquid chromatography (LC)-MS analysis, and the MS of the Cm hydrolysate was apparently identical to that of p -nitrophenylserinol (Sigma-Aldrich, St. Louis, MO), which was at m/z 213.2 [M+H]+ ; its dehydrated product was at m/z 195.2 [M+H-H2 O]+ ( ).

    Cell Culture:

    Article Title: Urinary Metabolite Profiling Combined with Computational Analysis Predicts Interstitial Cystitis-Associated Candidate Biomarkers
    Article Snippet: Reagents Cell culture medium and heat-inactivated and dialyzed fetal bovine serum were purchased from Invitrogen (Carlsbad, CA). .. All reagents for sample preparation for NMR and liquid chromatography–mass spectrometry (LC–MS) analysis as well as tyramine and 2-oxoglutarate were obtained from Sigma-Aldrich.

    Purification:

    Article Title: Real-time analysis of the binding of fluorescent VEGF165a to VEGFR2 in living cells: Effect of receptor tyrosine kinase inhibitors and fate of internalized agonist-receptor complexes
    Article Snippet: .. 2.8 Liquid chromatography–tandem mass spectrometry (LC–MS/MS) analysis VEGF165 a-TMR and VEGF165 a purified in the same manner were suspended in 50 mM ammonium bicarbonate (0.1 mg/ml; Sigma-Aldrich, USA) and reduced with 25 mM TCEP at 50 °C for 1 h. Following reduction, the appropriate protease (LysC, GluC, or trypsin/LysC from Promega Corporation, USA) were added to the sample (1:20 enzyme/substrate ratio) and subjected to proteolysis for 3 h at 37 °C with shaking at 400 rpm (Eppendorf Thermomixer). .. Following this step, the reaction was quenched by the addition of formic acid (1% final concentration; Sigma-Aldrich, USA) and subjected to LC-MS/MS analysis on a Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Fisher Scientific, USA) operating with an MS resolution of 70,000.

    Article Title: Surface Enhanced Raman Spectroscopy for In-Field Detection of Pesticides: A Test on Dimethoate Residues in Water and on Olive Leaves
    Article Snippet: DMT (analytical standard quality) was purchased from Merck/Sigma-Aldrich (Darmstat, D.) and was used without further purification. .. Water used for preparation of the different solutions was LC-MS chromatography grade, obtained from Merck/Sigma-Aldrich.

    Produced:

    Article Title: Inactivation of Chloramphenicol and Florfenicol by a Novel Chloramphenicol Hydrolase
    Article Snippet: Large volumes of Cm (50 mg) were incubated with EstDL136, and the Cm hydrolysate (a newly produced compound detected on TLC; Rf = 0.23) was detected mainly in the butanol extract of the reaction mixture, wherein 14.2 mg of the hydrolysate was isolated further by preparative TLC. .. The Cm hydrolysate isolated was subjected to liquid chromatography (LC)-MS analysis, and the MS of the Cm hydrolysate was apparently identical to that of p -nitrophenylserinol (Sigma-Aldrich, St. Louis, MO), which was at m/z 213.2 [M+H]+ ; its dehydrated product was at m/z 195.2 [M+H-H2 O]+ ( ).

    Concentration Assay:

    Article Title: Dietary Leucine Supplement Ameliorates Hepatic Steatosis and Diabetic Nephropathy in db/db Mice
    Article Snippet: .. Liquid Chromatography/Time-of-Flight Mass Spectrometry (LC/TOF MS)–Based Metabolomics Analysis of Urine Samples Urine was normalized with water to contain a creatinine concentration of 50 ug/mL and was filtered with 0.2 μm filter (millipore) to remove particles. .. The clear diluent was analyzed using LC/TOFMS (Waters Corp.).

    Article Title: Real-time analysis of the binding of fluorescent VEGF165a to VEGFR2 in living cells: Effect of receptor tyrosine kinase inhibitors and fate of internalized agonist-receptor complexes
    Article Snippet: 2.8 Liquid chromatography–tandem mass spectrometry (LC–MS/MS) analysis VEGF165 a-TMR and VEGF165 a purified in the same manner were suspended in 50 mM ammonium bicarbonate (0.1 mg/ml; Sigma-Aldrich, USA) and reduced with 25 mM TCEP at 50 °C for 1 h. Following reduction, the appropriate protease (LysC, GluC, or trypsin/LysC from Promega Corporation, USA) were added to the sample (1:20 enzyme/substrate ratio) and subjected to proteolysis for 3 h at 37 °C with shaking at 400 rpm (Eppendorf Thermomixer). .. Following this step, the reaction was quenched by the addition of formic acid (1% final concentration; Sigma-Aldrich, USA) and subjected to LC-MS/MS analysis on a Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Fisher Scientific, USA) operating with an MS resolution of 70,000.

    Generated:

    Article Title: Inactivation of Chloramphenicol and Florfenicol by a Novel Chloramphenicol Hydrolase
    Article Snippet: The Cm hydrolysate isolated was subjected to liquid chromatography (LC)-MS analysis, and the MS of the Cm hydrolysate was apparently identical to that of p -nitrophenylserinol (Sigma-Aldrich, St. Louis, MO), which was at m/z 213.2 [M+H]+ ; its dehydrated product was at m/z 195.2 [M+H-H2 O]+ ( ). .. These results with LC-MS and NMR strongly suggested that the structures of Cm hydrolysate and p -nitrophenylserinol are identical; therefore, we concluded that the hydrolysate of Cm is p -nitrophenylserinol, which is generated by hydrolyzing the amide linkage of Cm.

    Thin Layer Chromatography:

    Article Title: Inactivation of Chloramphenicol and Florfenicol by a Novel Chloramphenicol Hydrolase
    Article Snippet: Large volumes of Cm (50 mg) were incubated with EstDL136, and the Cm hydrolysate (a newly produced compound detected on TLC; Rf = 0.23) was detected mainly in the butanol extract of the reaction mixture, wherein 14.2 mg of the hydrolysate was isolated further by preparative TLC. .. The Cm hydrolysate isolated was subjected to liquid chromatography (LC)-MS analysis, and the MS of the Cm hydrolysate was apparently identical to that of p -nitrophenylserinol (Sigma-Aldrich, St. Louis, MO), which was at m/z 213.2 [M+H]+ ; its dehydrated product was at m/z 195.2 [M+H-H2 O]+ ( ).

    Nuclear Magnetic Resonance:

    Article Title: Urinary Metabolite Profiling Combined with Computational Analysis Predicts Interstitial Cystitis-Associated Candidate Biomarkers
    Article Snippet: .. All reagents for sample preparation for NMR and liquid chromatography–mass spectrometry (LC–MS) analysis as well as tyramine and 2-oxoglutarate were obtained from Sigma-Aldrich. ..

    Article Title: Biosynthetically-Intriguing Chlorinated Lipophilic Metabolites from Geographically Distant Tropical Marine Cyanobacteria
    Article Snippet: .. Based on preliminary 1 H NMR and LCMS analyses indicating that the extracts were virtually identical, the crude extracts were combined and fractionated using vacuum liquid chromatography (VLC) on Silica gel (Type H, 10–40 µM, Sigma-Aldrich) with a stepwise gradient of hexanes/EtOAc and EtOAc/MeOH to give nine fractions (A–I). .. Fraction D, eluted with 40% EtOAc in hexanes (159 mg) was subjected to two iterations of normal-phase chromatography using Si Varian Bond Elut Sep-Paks.

    Article Title: Inactivation of Chloramphenicol and Florfenicol by a Novel Chloramphenicol Hydrolase
    Article Snippet: The Cm hydrolysate isolated was subjected to liquid chromatography (LC)-MS analysis, and the MS of the Cm hydrolysate was apparently identical to that of p -nitrophenylserinol (Sigma-Aldrich, St. Louis, MO), which was at m/z 213.2 [M+H]+ ; its dehydrated product was at m/z 195.2 [M+H-H2 O]+ ( ). .. These results with LC-MS and NMR strongly suggested that the structures of Cm hydrolysate and p -nitrophenylserinol are identical; therefore, we concluded that the hydrolysate of Cm is p -nitrophenylserinol, which is generated by hydrolyzing the amide linkage of Cm.

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Urinary Metabolite Profiling Combined with Computational Analysis Predicts Interstitial Cystitis-Associated Candidate Biomarkers
    Article Snippet: .. All reagents for sample preparation for NMR and liquid chromatography–mass spectrometry (LC–MS) analysis as well as tyramine and 2-oxoglutarate were obtained from Sigma-Aldrich. ..

    Article Title: Neonatal Screening for Congenital Adrenal Hyperplasia in Turkey: A Pilot Study with 38,935 Infants
    Article Snippet: Reagent, Instruments, and Analytical Conditions for Liquid Chromatography-tandem Mass Spectrometry The steroid standards for F, 17-OHP, 21-S, 11-S, 4AS and deuterated steroid standards for d4-F, d8-17-OHP, d3-testosterone were purchased from Sigma-Aldrich (MO, USA). .. Acetonitrile (ACN), methanol, ethanol, isopropyl alcohol (IPA), formic acid and LC-MS grade water were purchased from Merck (Darmstadt, Germany).

    Article Title: Biosynthetically-Intriguing Chlorinated Lipophilic Metabolites from Geographically Distant Tropical Marine Cyanobacteria
    Article Snippet: .. Based on preliminary 1 H NMR and LCMS analyses indicating that the extracts were virtually identical, the crude extracts were combined and fractionated using vacuum liquid chromatography (VLC) on Silica gel (Type H, 10–40 µM, Sigma-Aldrich) with a stepwise gradient of hexanes/EtOAc and EtOAc/MeOH to give nine fractions (A–I). .. Fraction D, eluted with 40% EtOAc in hexanes (159 mg) was subjected to two iterations of normal-phase chromatography using Si Varian Bond Elut Sep-Paks.

    Article Title: Transfer and Metabolism of the Xenoestrogen Zearalenone in Human Perfused Placenta
    Article Snippet: .. Chemicals and Reagents Liquid chromatography–mass spectrometry (LC-MS)-grade acetonitrile (ACN) and methanol (MeOH) were obtained from Sigma-Aldrich, and water ( H 2 O , LC-MS grade) was purchased from VWR International GmbH. .. Ammonium fluoride (LC-MS grade) was obtained from Honeywell Fluka.

    Article Title: Time-series lipidomic analysis of the oleaginous green microalga species Ettlia oleoabundans under nutrient stress
    Article Snippet: .. Liquid chromatography - mass spectrometry (LC–MS) grade methanol and high-performance liquid chromatography (HPLC) grade isopropanol were purchased from Millipore Sigma (Billerica, MA). .. Formic acid, ammonium formate, and ammonium hydroxide were purchased from Sigma-Aldrich (St. Louis, MO).

    Article Title: Inactivation of Chloramphenicol and Florfenicol by a Novel Chloramphenicol Hydrolase
    Article Snippet: .. The Cm hydrolysate isolated was subjected to liquid chromatography (LC)-MS analysis, and the MS of the Cm hydrolysate was apparently identical to that of p -nitrophenylserinol (Sigma-Aldrich, St. Louis, MO), which was at m/z 213.2 [M+H]+ ; its dehydrated product was at m/z 195.2 [M+H-H2 O]+ ( ). .. In addition, 1 H-NMR analysis demonstrated that the chemical shift of the Cm hydrolysate and p -nitrophenylserinol peaks were identical ( and ).

    Article Title: Surface Enhanced Raman Spectroscopy for In-Field Detection of Pesticides: A Test on Dimethoate Residues in Water and on Olive Leaves
    Article Snippet: .. Water used for preparation of the different solutions was LC-MS chromatography grade, obtained from Merck/Sigma-Aldrich. ..

    Software:

    Article Title: Real-time analysis of the binding of fluorescent VEGF165a to VEGFR2 in living cells: Effect of receptor tyrosine kinase inhibitors and fate of internalized agonist-receptor complexes
    Article Snippet: 2.8 Liquid chromatography–tandem mass spectrometry (LC–MS/MS) analysis VEGF165 a-TMR and VEGF165 a purified in the same manner were suspended in 50 mM ammonium bicarbonate (0.1 mg/ml; Sigma-Aldrich, USA) and reduced with 25 mM TCEP at 50 °C for 1 h. Following reduction, the appropriate protease (LysC, GluC, or trypsin/LysC from Promega Corporation, USA) were added to the sample (1:20 enzyme/substrate ratio) and subjected to proteolysis for 3 h at 37 °C with shaking at 400 rpm (Eppendorf Thermomixer). .. MS-product component of the Protein Prospector software (University of California, USA) was used to calculate the mono-isotopic masses of the modified and unmodified peptides at different charge states (Z = 2–5 were considered; ).

    Liquid Chromatography:

    Article Title: Multi-modal mass spectrometric imaging of small molecules reveals distinct spatio-molecular signatures in differentially metastatic breast tumor models
    Article Snippet: .. For Matrix Enhanced (ME)-SIMS and MALDI-MS, the tissues were covered with 2,5-Dihydroxybenzoic acid (DHB) 30mg/mL in 50% acetonitrile/0.1% aqueous trifluoroacetic acid using air driven thin liquid chromatography sprayer (Sigma). ..

    Article Title: Response of Porphyromonas gingivalis to Heme Limitation in Continuous Culture ▿ to Heme Limitation in Continuous Culture ▿ †
    Article Snippet: .. Chemicals used were ultra-high purity, except for MS work, where liquid chromatography (LC)-MS-grade reagents were used (Sigma). .. P. gingivalis W50 was grown in continuous culture in a Bioflo 110 fermentor/bioreactor (New Brunswick Scientific) with a 400-ml working volume.

    Article Title: Biosynthetically-Intriguing Chlorinated Lipophilic Metabolites from Geographically Distant Tropical Marine Cyanobacteria
    Article Snippet: .. Based on preliminary 1 H NMR and LCMS analyses indicating that the extracts were virtually identical, the crude extracts were combined and fractionated using vacuum liquid chromatography (VLC) on Silica gel (Type H, 10–40 µM, Sigma-Aldrich) with a stepwise gradient of hexanes/EtOAc and EtOAc/MeOH to give nine fractions (A–I). .. Fraction D, eluted with 40% EtOAc in hexanes (159 mg) was subjected to two iterations of normal-phase chromatography using Si Varian Bond Elut Sep-Paks.

    Article Title: Time-series lipidomic analysis of the oleaginous green microalga species Ettlia oleoabundans under nutrient stress
    Article Snippet: .. Liquid chromatography - mass spectrometry (LC–MS) grade methanol and high-performance liquid chromatography (HPLC) grade isopropanol were purchased from Millipore Sigma (Billerica, MA). .. Formic acid, ammonium formate, and ammonium hydroxide were purchased from Sigma-Aldrich (St. Louis, MO).

    Article Title: Inactivation of Chloramphenicol and Florfenicol by a Novel Chloramphenicol Hydrolase
    Article Snippet: .. The Cm hydrolysate isolated was subjected to liquid chromatography (LC)-MS analysis, and the MS of the Cm hydrolysate was apparently identical to that of p -nitrophenylserinol (Sigma-Aldrich, St. Louis, MO), which was at m/z 213.2 [M+H]+ ; its dehydrated product was at m/z 195.2 [M+H-H2 O]+ ( ). .. In addition, 1 H-NMR analysis demonstrated that the chemical shift of the Cm hydrolysate and p -nitrophenylserinol peaks were identical ( and ).

    Imaging:

    Article Title: Multi-modal mass spectrometric imaging of small molecules reveals distinct spatio-molecular signatures in differentially metastatic breast tumor models
    Article Snippet: After the sections reached room temperature, different sample preparation procedures were applied depending on the MS imaging techniques used in this study. .. For Matrix Enhanced (ME)-SIMS and MALDI-MS, the tissues were covered with 2,5-Dihydroxybenzoic acid (DHB) 30mg/mL in 50% acetonitrile/0.1% aqueous trifluoroacetic acid using air driven thin liquid chromatography sprayer (Sigma).

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