Structured Review

SCIEX proteinpilot
Comparison of lists of protein identifications (IDs) derived from in-solution versus in-gel digestion using BioVenn Software [ 51 ]. Proteins were identified by searching a composite database of Bos taurus, ovis aries and Capra hircus using <t>ProteinPilot™</t> Software. Only 17 protein IDs were exclusive to in-solution workflow compared to 182 protein IDs exclusive to in-gel workflow
Proteinpilot, supplied by SCIEX, used in various techniques. Bioz Stars score: 99/100, based on 380 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/proteinpilot/product/SCIEX
Average 99 stars, based on 380 article reviews
Price from $9.99 to $1999.99
proteinpilot - by Bioz Stars, 2020-10
99/100 stars

Images

1) Product Images from "Characterisation of the circulating acellular proteome of healthy sheep using LC-MS/MS-based proteomics analysis of serum"

Article Title: Characterisation of the circulating acellular proteome of healthy sheep using LC-MS/MS-based proteomics analysis of serum

Journal: Proteome Science

doi: 10.1186/s12953-017-0119-z

Comparison of lists of protein identifications (IDs) derived from in-solution versus in-gel digestion using BioVenn Software [ 51 ]. Proteins were identified by searching a composite database of Bos taurus, ovis aries and Capra hircus using ProteinPilot™ Software. Only 17 protein IDs were exclusive to in-solution workflow compared to 182 protein IDs exclusive to in-gel workflow
Figure Legend Snippet: Comparison of lists of protein identifications (IDs) derived from in-solution versus in-gel digestion using BioVenn Software [ 51 ]. Proteins were identified by searching a composite database of Bos taurus, ovis aries and Capra hircus using ProteinPilot™ Software. Only 17 protein IDs were exclusive to in-solution workflow compared to 182 protein IDs exclusive to in-gel workflow

Techniques Used: Derivative Assay, Software

2) Product Images from "Identification of chemoresistant factors by protein expression analysis with iTRAQ for head and neck carcinoma"

Article Title: Identification of chemoresistant factors by protein expression analysis with iTRAQ for head and neck carcinoma

Journal: British Journal of Cancer

doi: 10.1038/bjc.2014.395

Flowchart of iTRAQ proteomics approach. Samples were treated according to the iTRAQ protocol, including the blocking of cysteines. They were then labelled with iTRAQ tags and analysed by the nano-LC and Triple TOF5600 system. Protein expression was confirmed by western blotting.
Figure Legend Snippet: Flowchart of iTRAQ proteomics approach. Samples were treated according to the iTRAQ protocol, including the blocking of cysteines. They were then labelled with iTRAQ tags and analysed by the nano-LC and Triple TOF5600 system. Protein expression was confirmed by western blotting.

Techniques Used: Blocking Assay, Expressing, Western Blot

Related Articles

Labeling:

Article Title: Quantitative Proteomics Reveals the Temperature-Dependent Proteins Encoded by a Series of Cluster Genes in Thermoanaerobacter Tengcongensis *
Article Snippet: .. For the sake of high quality in quantitative analysis, especially for the T. tengcongensis peptides labeled with iTRAQ, three software programs, Isobar , IsobariQ , and ProteinPilot (AB Sciex, Foster City, CA), were carefully evaluated against the same original MS/MS data. .. Based on the software analysis, the CVs distribution of all the quantified proteins and the quantitative results derived from duplicated injections were compared in parallel.

other:

Article Title: S-Trap eliminates cell culture media polymeric surfactants for effective proteomic analysis of mammalian cell bioreactor supernatants
Article Snippet: The following parameters were used to search in ProteinPilot: Sample type: identification; cysteine alkylation: acrylamide; digestion: trypsin; instrument: TripleTOF 5600; ID focus: biological modifications; search effort: thorough; FDR analysis: 1% global.

Mass Spectrometry:

Article Title: Quantitative Proteomics by SWATH-MS Reveals Altered Expression of Nucleic Acid Binding and Regulatory Proteins in HIV-1-Infected Macrophages
Article Snippet: .. Generating the MDM Reference Spectral Library All DDA mass spectrometry files were searched in unison using ProteinPilot software v. 4.2 (AB Sciex) with the Paragon algorithm. ..

Article Title: Identification of chemoresistant factors by protein expression analysis with iTRAQ for head and neck carcinoma
Article Snippet: .. Protein identification and quantification MS and MS/MS data searches were carried out using ProteinPilot software (4.0.8085; AB Sciex) based on the workflow with a spectrum selector and reporter ion quantifier. ..

Tandem Mass Spectroscopy:

Article Title: Quantitative Proteomics Reveals the Temperature-Dependent Proteins Encoded by a Series of Cluster Genes in Thermoanaerobacter Tengcongensis *
Article Snippet: .. For the sake of high quality in quantitative analysis, especially for the T. tengcongensis peptides labeled with iTRAQ, three software programs, Isobar , IsobariQ , and ProteinPilot (AB Sciex, Foster City, CA), were carefully evaluated against the same original MS/MS data. .. Based on the software analysis, the CVs distribution of all the quantified proteins and the quantitative results derived from duplicated injections were compared in parallel.

Article Title: Identification of chemoresistant factors by protein expression analysis with iTRAQ for head and neck carcinoma
Article Snippet: .. Protein identification and quantification MS and MS/MS data searches were carried out using ProteinPilot software (4.0.8085; AB Sciex) based on the workflow with a spectrum selector and reporter ion quantifier. ..

Modification:

Article Title: Data on assessment of safety and tear proteome change in response to orthokeratology lens – Insight from integrating clinical data and next generation proteomics
Article Snippet: .. The data were searched against Homo Sapiens Uniprot database [ ] and protein identification (ID) was acquired using ProteinPilot (v5.0.1, Sciex) software with Paragon algorithms with the following settings: Trypsin as the enzyme, cysteine alkylation using iodoacetamide (IAA), thorough search effort and biological modification. ..

Software:

Article Title: Characterisation of the circulating acellular proteome of healthy sheep using LC-MS/MS-based proteomics analysis of serum
Article Snippet: .. Proteins were identified using ProteinPilot™ (SCIEX) and Mascot (Matrix Science) software based on a minimum of two unmodified highly scoring unique peptides per protein at a false discovery rate (FDR) of 1% software by searching a subset of the Universal Protein Resource Knowledgebase (UniProtKB) database ( http://www.uniprot.org ). ..

Article Title: Quantitative Proteomics Reveals the Temperature-Dependent Proteins Encoded by a Series of Cluster Genes in Thermoanaerobacter Tengcongensis *
Article Snippet: .. For the sake of high quality in quantitative analysis, especially for the T. tengcongensis peptides labeled with iTRAQ, three software programs, Isobar , IsobariQ , and ProteinPilot (AB Sciex, Foster City, CA), were carefully evaluated against the same original MS/MS data. .. Based on the software analysis, the CVs distribution of all the quantified proteins and the quantitative results derived from duplicated injections were compared in parallel.

Article Title: Data on assessment of safety and tear proteome change in response to orthokeratology lens – Insight from integrating clinical data and next generation proteomics
Article Snippet: .. The data were searched against Homo Sapiens Uniprot database [ ] and protein identification (ID) was acquired using ProteinPilot (v5.0.1, Sciex) software with Paragon algorithms with the following settings: Trypsin as the enzyme, cysteine alkylation using iodoacetamide (IAA), thorough search effort and biological modification. ..

Article Title: Quantitative Proteomics by SWATH-MS Reveals Altered Expression of Nucleic Acid Binding and Regulatory Proteins in HIV-1-Infected Macrophages
Article Snippet: .. Generating the MDM Reference Spectral Library All DDA mass spectrometry files were searched in unison using ProteinPilot software v. 4.2 (AB Sciex) with the Paragon algorithm. ..

Article Title: Quantitative Targeted Proteomics for Membrane Transporter Proteins: Method and Application
Article Snippet: .. Many vendors also have their own processing software such as ProteinPilot of AB Sciex (Fig. ), ProteinLynx of Waters, and Proteome Discoverer of Thermo Scientific. .. As exemplified by the ProteinPilot of AB Sciex, the software offers better working interfaces and data visualization and can be used by personal preference (Fig. ).

Article Title: Identification of chemoresistant factors by protein expression analysis with iTRAQ for head and neck carcinoma
Article Snippet: .. Protein identification and quantification MS and MS/MS data searches were carried out using ProteinPilot software (4.0.8085; AB Sciex) based on the workflow with a spectrum selector and reporter ion quantifier. ..

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    SCIEX liquid chromatography electrospray ionization tandem mass spectrometry
    Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry, supplied by SCIEX, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/liquid chromatography electrospray ionization tandem mass spectrometry/product/SCIEX
    Average 90 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    liquid chromatography electrospray ionization tandem mass spectrometry - by Bioz Stars, 2020-10
    90/100 stars
      Buy from Supplier

    Image Search Results