lipid mediated dna transfection  (Thermo Fisher)


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    Structured Review

    Thermo Fisher lipid mediated dna transfection
    Lipid Mediated Dna Transfection, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipid mediated dna transfection/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lipid mediated dna transfection - by Bioz Stars, 2020-09
    88/100 stars

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    Related Articles

    Transfection:

    Article Title: Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) Downregulates the Expression of Protumor Factors Cyclooxygenase-2 and Inducible Nitric Oxide Synthase in a GM-CSF Receptor-Independent Manner in Cervical Cancer Cells
    Article Snippet: .. To establish overexpression of GM-CSF in the cervical cancer cell lines, the hGM-CSF-expressing plasmid pcDNA3.1(−)/GM-CSF (hereafter referred to as pGM-CSF) and the vector pcDNA3.1(−) were purchased from Invitrogen (San Diego, CA, USA) and lipid-mediated DNA transfection (Lipofectamine 2000; Invitrogen) was performed. .. To silence the expression of hGM-CSF, the cancer cell lines were seeded in 6-well tissue culture plates (105 cells/mL) and transfected using the Lipofectamine 2000 Reagent to introduce 5 nM of GM-CSF siRNA (number sc39391; Santa Cruz Biotech) or scrambled control siRNA (number sc-37007, Santa Cruz Biotech).

    Over Expression:

    Article Title: Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) Downregulates the Expression of Protumor Factors Cyclooxygenase-2 and Inducible Nitric Oxide Synthase in a GM-CSF Receptor-Independent Manner in Cervical Cancer Cells
    Article Snippet: .. To establish overexpression of GM-CSF in the cervical cancer cell lines, the hGM-CSF-expressing plasmid pcDNA3.1(−)/GM-CSF (hereafter referred to as pGM-CSF) and the vector pcDNA3.1(−) were purchased from Invitrogen (San Diego, CA, USA) and lipid-mediated DNA transfection (Lipofectamine 2000; Invitrogen) was performed. .. To silence the expression of hGM-CSF, the cancer cell lines were seeded in 6-well tissue culture plates (105 cells/mL) and transfected using the Lipofectamine 2000 Reagent to introduce 5 nM of GM-CSF siRNA (number sc39391; Santa Cruz Biotech) or scrambled control siRNA (number sc-37007, Santa Cruz Biotech).

    Plasmid Preparation:

    Article Title: Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) Downregulates the Expression of Protumor Factors Cyclooxygenase-2 and Inducible Nitric Oxide Synthase in a GM-CSF Receptor-Independent Manner in Cervical Cancer Cells
    Article Snippet: .. To establish overexpression of GM-CSF in the cervical cancer cell lines, the hGM-CSF-expressing plasmid pcDNA3.1(−)/GM-CSF (hereafter referred to as pGM-CSF) and the vector pcDNA3.1(−) were purchased from Invitrogen (San Diego, CA, USA) and lipid-mediated DNA transfection (Lipofectamine 2000; Invitrogen) was performed. .. To silence the expression of hGM-CSF, the cancer cell lines were seeded in 6-well tissue culture plates (105 cells/mL) and transfected using the Lipofectamine 2000 Reagent to introduce 5 nM of GM-CSF siRNA (number sc39391; Santa Cruz Biotech) or scrambled control siRNA (number sc-37007, Santa Cruz Biotech).

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    Thermo Fisher lipofectamine 3000 transfection reagent
    Lipofectamine 3000 Transfection Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 292 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipofectamine 3000 transfection reagent/product/Thermo Fisher
    Average 99 stars, based on 292 article reviews
    Price from $9.99 to $1999.99
    lipofectamine 3000 transfection reagent - by Bioz Stars, 2020-09
    99/100 stars
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