lipid mediated cell transfection lipofectamine 3000  (Thermo Fisher)


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    Name:
    Lipofectamine 3000 Transfection Reagent
    Description:
    Lipofectamine 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results This reagent delivers superior transfection efficiency and improved cell viability for the widest range of hard to transfect and common cells e g HEK293 HeLa Successfully transfect the widest variety of biologically relevant cell types with a reagent that offers • Superior performance our highest efficiency reagent for difficult to transfect cells• Improved cell viability gentle on your cells with low toxicity• Versatile one reagent for DNA RNA and co transfection Efficiently Transfect Difficult to Transfect Cells Lipofectamine 3000 reagent is designed to efficiently transfect difficult to transfect cells yielding superior transfection performance across the broadest array of cell types Successfully transfected cell types include Fibroblast 3T3 COS 7 Myoblast C2C12 L6 CRL 1458 Hepatocyte HepG2 HuH7 Erythroleukemic cell K562 Breast carcinoma MCF7 Hs578T Prostate carcinoma LNCap Lung carcinoma A549 NCI H460 Osteosarcoma U 2 OS Saos 2 Colon carcinoma Caco2 SW480 Pancreatic carcinoma PANC 1 Skin melanoma SK MEL 28 View performance data for 60 cell lines plus comparison to Lipofectamine 2000 Transfect Common Cells Efficiently and Cost EffectivelyFor common cell types Lipofectamine 3000 reagent is more cost effective than other reagents due to its high concentration and low usage amount The 1 5 mL size provides up to 1500 transfection reactions in 24 well plates Achieve Superior Transfection Efficiency While Maintaining Low Toxicity Lipofectamine 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization A low toxicity lipid dose 0 75 µL for 24 well plates is suggested for applications requiring minimal disruption of the cells Enable High Transfection Efficiency in Novel Genome Editing ApplicationsLipofectamine 3000 reagent is suitable for novel genome editing applications It increases the likelihood of successful cleavage and recombination with TALENs or CRISPRs through highly efficient transfection ultimately maximizing the efficiency of genetic modifications and simplifying the downstream processes
    Catalog Number:
    l3000001
    Price:
    None
    Applications:
    Cell Culture|Plasmid Transfection|Transfection
    Category:
    Cell Culture Transfection Reagents
    Buy from Supplier


    Structured Review

    Thermo Fisher lipid mediated cell transfection lipofectamine 3000
    Lipofectamine 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results This reagent delivers superior transfection efficiency and improved cell viability for the widest range of hard to transfect and common cells e g HEK293 HeLa Successfully transfect the widest variety of biologically relevant cell types with a reagent that offers • Superior performance our highest efficiency reagent for difficult to transfect cells• Improved cell viability gentle on your cells with low toxicity• Versatile one reagent for DNA RNA and co transfection Efficiently Transfect Difficult to Transfect Cells Lipofectamine 3000 reagent is designed to efficiently transfect difficult to transfect cells yielding superior transfection performance across the broadest array of cell types Successfully transfected cell types include Fibroblast 3T3 COS 7 Myoblast C2C12 L6 CRL 1458 Hepatocyte HepG2 HuH7 Erythroleukemic cell K562 Breast carcinoma MCF7 Hs578T Prostate carcinoma LNCap Lung carcinoma A549 NCI H460 Osteosarcoma U 2 OS Saos 2 Colon carcinoma Caco2 SW480 Pancreatic carcinoma PANC 1 Skin melanoma SK MEL 28 View performance data for 60 cell lines plus comparison to Lipofectamine 2000 Transfect Common Cells Efficiently and Cost EffectivelyFor common cell types Lipofectamine 3000 reagent is more cost effective than other reagents due to its high concentration and low usage amount The 1 5 mL size provides up to 1500 transfection reactions in 24 well plates Achieve Superior Transfection Efficiency While Maintaining Low Toxicity Lipofectamine 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization A low toxicity lipid dose 0 75 µL for 24 well plates is suggested for applications requiring minimal disruption of the cells Enable High Transfection Efficiency in Novel Genome Editing ApplicationsLipofectamine 3000 reagent is suitable for novel genome editing applications It increases the likelihood of successful cleavage and recombination with TALENs or CRISPRs through highly efficient transfection ultimately maximizing the efficiency of genetic modifications and simplifying the downstream processes
    https://www.bioz.com/result/lipid mediated cell transfection lipofectamine 3000/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    lipid mediated cell transfection lipofectamine 3000 - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Transfection:

    Article Title: Spred2 Regulates High Fat Diet-Induced Adipose Tissue Inflammation, and Metabolic Abnormalities in Mice
    Article Snippet: .. For each transfection, 0.75 μL Lipofectamine 3000 (Life Technologies, Carlsbad, CA, USA) and 0.5 μg Spred2 expression plasmid (kindly provided by Dr. Masakiyo Sakaguchi, Okayama university, Japan) were added to 50 μL Opti-MEM (Life Technologies) and incubated for 10 min at room temperature before the mixtures were added to the cells. .. Cells that overexpressed Spred2 were grown in RPMI-1640 medium with 10% FBS for 24 h. Cells were then incubated overnight in serum-free RPMI-1640 medium, after which the cells were cultured with BSA-conjugated PA (500 μM) for 24 h.

    Article Title: Systematic Screening of Commonly Used Commercial Transfection Reagents towards Efficient Transfection of Single-Stranded Oligonucleotides
    Article Snippet: .. Transfection Reagents and SSO RNAiMAX transfection reagent (13778030, Invitrogen, Waltham, MA, USA), Lipofectamine 3000 (L3000001, Invitrogen), FuGENE transfection reagent (E2311, Promega, Sydney, Australia), Lipofectamine 2000 reagent (11668027, Invitrogen), Lipofectin reagent (18292011, Invitrogen) were used in this study. .. To evaluate the efficacy of transfection reagents to SSO, a 5′ end FAM labelled 24-mer scrambled ssDNA sequence (5′-FAM-CATCGATGGGAGCTCCGTGTCGTT-3′) was synthesized by Integrated DNA Technologies (IDT, Singapore).

    Article Title: Amino acid substitutions at sugar-recognizing codons confer ABO blood group system-related α1,3 Gal(NAc) transferases with differential enzymatic activity
    Article Snippet: .. DNA transfection experiments For immunocytochemistry experiments DNA transfection was performed using Lipofectamine 3000 reagents, following the manufacturer’s protocol (Thermo Fisher Scientific). .. In order to normalize transfection efficiency, a fixed amount of DNA from pEGFP construct expressing enhanced green fluorescent protein (GFP) was co-transfected.

    Article Title: CRISPR/Cas9-Mediated Genomic Deletion of the Beta-1, 4 N-acetylgalactosaminyltransferase 1 Gene in Murine P19 Embryonal Carcinoma Cells Results in Low Sensitivity to Botulinum Neurotoxin Type C
    Article Snippet: .. Generation of GalNAc-T-depleted P19 cells Undifferentiated P19 cells were transfected with the CRISPR/Cas9 vector and Lipofectamine 3000 (Life Technologies) according to the manufacturer’s instructions. .. Cells were collected by trypsinization at 48 h after transfection.

    Article Title: Cryo-EM structure of substrate-bound human telomerase holoenzyme
    Article Snippet: .. Genome engineering in HCT116 cells was performed using the manufacturer’s transfection protocol (Lipofectamine 3000, Thermo) with media exchange at 24 hours into selection with 200 μg/ml of G418 and 100 μg/ml of hygromycin. .. Cells were passaged continuously with selection media for > 3 months after selection for cells with the integrated TERT transgenes.

    Article Title: JIP3 Activates Kinesin-1 Motility to Promote Axon Elongation *
    Article Snippet: .. Cells were transfected using Lipofectamine 2000 (KLC-mCit, all FLAG-JIP3 and GFP-JIP3 constructs) or Lipofectamine 3000 (FLAG-KHC), both from Invitrogen, according to the manufacturer's instructions. .. Twenty-four hours after transfection, COS7 cells were lysed in ice-cold buffer (500 m m PIPES, 1 m m EGTA, 1 m m MgSO4 , 0.1% Triton X-100, Roche protease inhibitor tablet, pH 6.9), and supernatant was collected by centrifugation at 16,000 × g for 10 min at 4 °C.

    Immunocytochemistry:

    Article Title: Amino acid substitutions at sugar-recognizing codons confer ABO blood group system-related α1,3 Gal(NAc) transferases with differential enzymatic activity
    Article Snippet: .. DNA transfection experiments For immunocytochemistry experiments DNA transfection was performed using Lipofectamine 3000 reagents, following the manufacturer’s protocol (Thermo Fisher Scientific). .. In order to normalize transfection efficiency, a fixed amount of DNA from pEGFP construct expressing enhanced green fluorescent protein (GFP) was co-transfected.

    Construct:

    Article Title: JIP3 Activates Kinesin-1 Motility to Promote Axon Elongation *
    Article Snippet: .. Cells were transfected using Lipofectamine 2000 (KLC-mCit, all FLAG-JIP3 and GFP-JIP3 constructs) or Lipofectamine 3000 (FLAG-KHC), both from Invitrogen, according to the manufacturer's instructions. .. Twenty-four hours after transfection, COS7 cells were lysed in ice-cold buffer (500 m m PIPES, 1 m m EGTA, 1 m m MgSO4 , 0.1% Triton X-100, Roche protease inhibitor tablet, pH 6.9), and supernatant was collected by centrifugation at 16,000 × g for 10 min at 4 °C.

    Incubation:

    Article Title: Spred2 Regulates High Fat Diet-Induced Adipose Tissue Inflammation, and Metabolic Abnormalities in Mice
    Article Snippet: .. For each transfection, 0.75 μL Lipofectamine 3000 (Life Technologies, Carlsbad, CA, USA) and 0.5 μg Spred2 expression plasmid (kindly provided by Dr. Masakiyo Sakaguchi, Okayama university, Japan) were added to 50 μL Opti-MEM (Life Technologies) and incubated for 10 min at room temperature before the mixtures were added to the cells. .. Cells that overexpressed Spred2 were grown in RPMI-1640 medium with 10% FBS for 24 h. Cells were then incubated overnight in serum-free RPMI-1640 medium, after which the cells were cultured with BSA-conjugated PA (500 μM) for 24 h.

    Article Title: Ehrlichia chaffeensis TRP120 Effector Targets and Recruits Host Polycomb Group Proteins for Degradation To Promote Intracellular Infection
    Article Snippet: .. Briefly, specific siRNA (3 μl) and Lipofectamine 3000 reagent (7.5 μl) were added to Opti-MEM medium (250 μl) (Invitrogen), incubated for 5 min at room temperature, and then added to the cell suspension in a 6-well plate. .. A control siRNA consisting of a scrambled sequence was used as a negative control, and a fluorescently labeled siRNA (Alexa Fluor 488-conjugated siRNA) was used as a control for transfection monitoring and optimization.

    Selection:

    Article Title: Cryo-EM structure of substrate-bound human telomerase holoenzyme
    Article Snippet: .. Genome engineering in HCT116 cells was performed using the manufacturer’s transfection protocol (Lipofectamine 3000, Thermo) with media exchange at 24 hours into selection with 200 μg/ml of G418 and 100 μg/ml of hygromycin. .. Cells were passaged continuously with selection media for > 3 months after selection for cells with the integrated TERT transgenes.

    Expressing:

    Article Title: Spred2 Regulates High Fat Diet-Induced Adipose Tissue Inflammation, and Metabolic Abnormalities in Mice
    Article Snippet: .. For each transfection, 0.75 μL Lipofectamine 3000 (Life Technologies, Carlsbad, CA, USA) and 0.5 μg Spred2 expression plasmid (kindly provided by Dr. Masakiyo Sakaguchi, Okayama university, Japan) were added to 50 μL Opti-MEM (Life Technologies) and incubated for 10 min at room temperature before the mixtures were added to the cells. .. Cells that overexpressed Spred2 were grown in RPMI-1640 medium with 10% FBS for 24 h. Cells were then incubated overnight in serum-free RPMI-1640 medium, after which the cells were cultured with BSA-conjugated PA (500 μM) for 24 h.

    CRISPR:

    Article Title: CRISPR/Cas9-Mediated Genomic Deletion of the Beta-1, 4 N-acetylgalactosaminyltransferase 1 Gene in Murine P19 Embryonal Carcinoma Cells Results in Low Sensitivity to Botulinum Neurotoxin Type C
    Article Snippet: .. Generation of GalNAc-T-depleted P19 cells Undifferentiated P19 cells were transfected with the CRISPR/Cas9 vector and Lipofectamine 3000 (Life Technologies) according to the manufacturer’s instructions. .. Cells were collected by trypsinization at 48 h after transfection.

    Plasmid Preparation:

    Article Title: Spred2 Regulates High Fat Diet-Induced Adipose Tissue Inflammation, and Metabolic Abnormalities in Mice
    Article Snippet: .. For each transfection, 0.75 μL Lipofectamine 3000 (Life Technologies, Carlsbad, CA, USA) and 0.5 μg Spred2 expression plasmid (kindly provided by Dr. Masakiyo Sakaguchi, Okayama university, Japan) were added to 50 μL Opti-MEM (Life Technologies) and incubated for 10 min at room temperature before the mixtures were added to the cells. .. Cells that overexpressed Spred2 were grown in RPMI-1640 medium with 10% FBS for 24 h. Cells were then incubated overnight in serum-free RPMI-1640 medium, after which the cells were cultured with BSA-conjugated PA (500 μM) for 24 h.

    Article Title: CRISPR/Cas9-Mediated Genomic Deletion of the Beta-1, 4 N-acetylgalactosaminyltransferase 1 Gene in Murine P19 Embryonal Carcinoma Cells Results in Low Sensitivity to Botulinum Neurotoxin Type C
    Article Snippet: .. Generation of GalNAc-T-depleted P19 cells Undifferentiated P19 cells were transfected with the CRISPR/Cas9 vector and Lipofectamine 3000 (Life Technologies) according to the manufacturer’s instructions. .. Cells were collected by trypsinization at 48 h after transfection.

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  • 99
    Thermo Fisher lipid mediated cell transfection lipofectamine 3000
    Lipid Mediated Cell Transfection Lipofectamine 3000, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipid mediated cell transfection lipofectamine 3000/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    lipid mediated cell transfection lipofectamine 3000 - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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