lipid based transfection reagent fugene 6 hd  (Promega)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    FuGENE 6 Transfection Reagent
    Description:
    Proven dependable performance Best for everyday use with common cell lines
    Catalog Number:
    e2691
    Price:
    None
    Category:
    Cell Biology Reporter Assays Transfection Transfection Reagents
    Buy from Supplier


    Structured Review

    Promega lipid based transfection reagent fugene 6 hd
    Proven dependable performance Best for everyday use with common cell lines
    https://www.bioz.com/result/lipid based transfection reagent fugene 6 hd/product/Promega
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lipid based transfection reagent fugene 6 hd - by Bioz Stars, 2020-08
    99/100 stars

    Images

    Related Articles

    Transfection:

    Article Title: Enhancing microparticle internalization by nonphagocytic cells through the use of noncovalently conjugated polyethyleneimine
    Article Snippet: .. PEI, LF2000, and FuGENE 6 cytotoxicity assay The cytotoxicity of PEI 25 KDa (Sigma-Aldrich, St Louis, MO) used at three different concentrations (0.05, 0.10, and 0.15 mM), of LF2000 (Life Technologies), and of FuGENE 6 (Promega Corporation, Fitchburg, WI), used at the concentrations recommended by the manufacturers, was evaluated after the transfection procedure by assessing two different parameters: the percentage of cells that remained attached to the dish (normalized to the control group), and the viability of the attached cells. ..

    Article Title: An improved method for increasing the efficiency of gene transfection and transduction
    Article Snippet: .. Transfection reagents lepofectamine 3000 and 2000 reagents were obtained from ThermoFisher, while FuGENE 6 was acquired from Promega (Madison, WI, USA). .. HEK293 and human umbilical vein endothelial cells (HUVECs) were obtained from ATCC (American Type Culture Collection, Manassas, Virginia, USA).

    Article Title: CRISPR/Cas9 engineering of a KIM-1 reporter human proximal tubule cell line
    Article Snippet: .. Cells were transfected with the pX330-U6-Chimeric_BB-CBh-hSpCas9 vector containing the gRNA and the GFP-T2A-Luc-pA-EF1α-RFP-T2A-Puro-MCS targeting vector with homology domains the following day using FuGENE 6 according to the manufacturer’s instructions (Promega, Madison, WI). .. RFP-positive clones were selected in HK-2 CM supplemented with 1–2 μg/mL puromycin.

    Article Title: Fetal vs adult mesenchymal stem cells achieve greater gene expression, but less osteoinduction
    Article Snippet: .. While we achieved around 35% transfection efficiency of pcDNA3-eGFP ratio, we found PEI to be relatively nontoxic in contrast to Lipofectamine and FuGENE 6. .. In conclusion, PEI offers an alternative to viral vector transduction and advantages over other chemical transfection reagents, especially when funding to support the use of viral vectors is not available.

    Article Title: Ghrelin Alleviates Angiotensin II-Induced H9c2 Apoptosis: Impact of the miR-208 Family
    Article Snippet: .. The FuGENE transfection reagent was purchased from Promega (Madison, WI, USA). .. PrimeScript™ RT reagent Kit and SYBR Premix Ex Taq™ (Tli RNaseH Plus) were provided by Takara (Kusatsu, Shiga, Japan).

    Article Title: Plasmid-based shRNA lentiviral particle production for RNAi applications
    Article Snippet: .. A master mix containing 16.5 μL of Opti-Mem (Life Technologies), 0.3 μL or 0.6 μL of FuGENE 6 and HD transfection reagent (Promega) diluted to 0.75 μL, and 1 μL of Lentiviral Packaging Mix was dispensed into an intermediate 96-well polypropylene storage plate (Corning 3357) containing 0.1 μg of expression plasmid and incubated for 15 minutes to promote plasmid-transfection reagent complex formation. ..

    Cytometry:

    Article Title: Immunoproteasome Subunits Are Required for CD8+ T Cell Function and Host Resistance to Brucella abortus Infection in Mice
    Article Snippet: .. A total of 5 × 105 cells/well of BMDCs from TKO and wild-type mice were plated into 96-well plates with 50 μg of OVA (Sigma, St. Louis, MO) and 1.25 μl of Fugene (Promega, Madison, WI) in 0.2 ml of DMEM–20 mM HEPES (pH 7.4) for 2 h at 37°C and used for analysis by flow cytometry. .. In the first step, cells were blocked with anti-mouse CD16/CD32 MAbs (Fc-Block), and the cells were then stained for CD11c, MHC-I–SIINFEKL, and MHC-II surface markers.

    Mouse Assay:

    Article Title: Immunoproteasome Subunits Are Required for CD8+ T Cell Function and Host Resistance to Brucella abortus Infection in Mice
    Article Snippet: .. A total of 5 × 105 cells/well of BMDCs from TKO and wild-type mice were plated into 96-well plates with 50 μg of OVA (Sigma, St. Louis, MO) and 1.25 μl of Fugene (Promega, Madison, WI) in 0.2 ml of DMEM–20 mM HEPES (pH 7.4) for 2 h at 37°C and used for analysis by flow cytometry. .. In the first step, cells were blocked with anti-mouse CD16/CD32 MAbs (Fc-Block), and the cells were then stained for CD11c, MHC-I–SIINFEKL, and MHC-II surface markers.

    Incubation:

    Article Title: Plasmid-based shRNA lentiviral particle production for RNAi applications
    Article Snippet: .. A master mix containing 16.5 μL of Opti-Mem (Life Technologies), 0.3 μL or 0.6 μL of FuGENE 6 and HD transfection reagent (Promega) diluted to 0.75 μL, and 1 μL of Lentiviral Packaging Mix was dispensed into an intermediate 96-well polypropylene storage plate (Corning 3357) containing 0.1 μg of expression plasmid and incubated for 15 minutes to promote plasmid-transfection reagent complex formation. ..

    Expressing:

    Article Title: Plasmid-based shRNA lentiviral particle production for RNAi applications
    Article Snippet: .. A master mix containing 16.5 μL of Opti-Mem (Life Technologies), 0.3 μL or 0.6 μL of FuGENE 6 and HD transfection reagent (Promega) diluted to 0.75 μL, and 1 μL of Lentiviral Packaging Mix was dispensed into an intermediate 96-well polypropylene storage plate (Corning 3357) containing 0.1 μg of expression plasmid and incubated for 15 minutes to promote plasmid-transfection reagent complex formation. ..

    Cytotoxicity Assay:

    Article Title: Enhancing microparticle internalization by nonphagocytic cells through the use of noncovalently conjugated polyethyleneimine
    Article Snippet: .. PEI, LF2000, and FuGENE 6 cytotoxicity assay The cytotoxicity of PEI 25 KDa (Sigma-Aldrich, St Louis, MO) used at three different concentrations (0.05, 0.10, and 0.15 mM), of LF2000 (Life Technologies), and of FuGENE 6 (Promega Corporation, Fitchburg, WI), used at the concentrations recommended by the manufacturers, was evaluated after the transfection procedure by assessing two different parameters: the percentage of cells that remained attached to the dish (normalized to the control group), and the viability of the attached cells. ..

    Plasmid Preparation:

    Article Title: CRISPR/Cas9 engineering of a KIM-1 reporter human proximal tubule cell line
    Article Snippet: .. Cells were transfected with the pX330-U6-Chimeric_BB-CBh-hSpCas9 vector containing the gRNA and the GFP-T2A-Luc-pA-EF1α-RFP-T2A-Puro-MCS targeting vector with homology domains the following day using FuGENE 6 according to the manufacturer’s instructions (Promega, Madison, WI). .. RFP-positive clones were selected in HK-2 CM supplemented with 1–2 μg/mL puromycin.

    Article Title: Plasmid-based shRNA lentiviral particle production for RNAi applications
    Article Snippet: .. A master mix containing 16.5 μL of Opti-Mem (Life Technologies), 0.3 μL or 0.6 μL of FuGENE 6 and HD transfection reagent (Promega) diluted to 0.75 μL, and 1 μL of Lentiviral Packaging Mix was dispensed into an intermediate 96-well polypropylene storage plate (Corning 3357) containing 0.1 μg of expression plasmid and incubated for 15 minutes to promote plasmid-transfection reagent complex formation. ..

    Flow Cytometry:

    Article Title: Immunoproteasome Subunits Are Required for CD8+ T Cell Function and Host Resistance to Brucella abortus Infection in Mice
    Article Snippet: .. A total of 5 × 105 cells/well of BMDCs from TKO and wild-type mice were plated into 96-well plates with 50 μg of OVA (Sigma, St. Louis, MO) and 1.25 μl of Fugene (Promega, Madison, WI) in 0.2 ml of DMEM–20 mM HEPES (pH 7.4) for 2 h at 37°C and used for analysis by flow cytometry. .. In the first step, cells were blocked with anti-mouse CD16/CD32 MAbs (Fc-Block), and the cells were then stained for CD11c, MHC-I–SIINFEKL, and MHC-II surface markers.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Promega lipid based transfection reagent fugene 6 hd
    Lipid Based Transfection Reagent Fugene 6 Hd, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipid based transfection reagent fugene 6 hd/product/Promega
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lipid based transfection reagent fugene 6 hd - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Promega lipid based transfection reagent fugene hd
    Lipid Based Transfection Reagent Fugene Hd, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipid based transfection reagent fugene hd/product/Promega
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lipid based transfection reagent fugene hd - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results