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Santa Cruz Biotechnology lipid based transfection agent
Lipid Based Transfection Agent, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lipid based transfection agent/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
lipid based transfection agent - by Bioz Stars, 2021-07
86/100 stars

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Transfection:

Article Title: HIV is inactivated after transepithelial migration via adult oral epithelial cells but not fetal epithelial cells
Article Snippet: To determine the functional role of innate proteins in HIV inactivation, expression of HBD1, HBD2, HBD3 and SLPI in polarized adult oral epithelial cells were inhibited using an siRNA approach. .. Polarized tongue cells were treated with lipid-based transfection reagents containing specific siRNAs (Santa Cruz Biotechnology Inc) against the mRNAs of each protein, as described by the manufacturer. .. As controls, unrelated (scrambled) siRNAs were used (Santa Cruz Biotechnology Inc).

Article Title: Mesenchymal stromal cell potency to treat acute kidney injury increased by ultrasound‐activated interferon‐γ/interleukin‐10 axis. Mesenchymal stromal cell potency to treat acute kidney injury increased by ultrasound‐activated interferon‐γ/interleukin‐10 axis
Article Snippet: IL‐10 expression was knocked down in MSC using multiple human‐IL‐10‐specific siRNA sequences (Cat. No. sc‐39634; Santa Cruz Biotechnology, Santa Cruz, CA). .. MSCs were plated at a density of 5.6 × 104 MSC/cm2 24 hours before siRNA treatment. siRNA was added to a lipid‐based transfection agent (Santa Cruz) and allowed to incubate at room temperature for 20 minutes before being diluted in siRNA transfection medium according to the manufacturer's protocol (Santa Cruz). siRNA was added to cells at 150 pg/106 MSC for 6 hours. siRNA‐containing medium was then diluted 1:2 with complete medium and cells were incubated another 24 hours. .. Cultures were then aspirated and MSCs were either prepared for animal infusions (see above) or incubated in cell lysis buffer (Cell Signaling Technology, Danvers, MA) containing a protease inhibitor cocktail (Santa Cruz) for IL‐10 expression analysis by western blot (see below).

Article Title: Involvement of PKC‐ι in glioma proliferation
Article Snippet: Sequence specific RNA 19–25 nucleotides in length were synthesized by Santa Cruz Biotechnology against PKC‐ι. .. PKC‐ι siRNA were transfected into glioma cells (U‐138MG and T98G) using lipid based siRNA transfection reagent (Santa Cruz Biotechnology). .. Sequence‐specific PKC siRNA were as follows: PKC‐ι siRNA is a pooled sequence that consists of three combined RNA sequences – mRNA location.

Incubation:

Article Title: Mesenchymal stromal cell potency to treat acute kidney injury increased by ultrasound‐activated interferon‐γ/interleukin‐10 axis. Mesenchymal stromal cell potency to treat acute kidney injury increased by ultrasound‐activated interferon‐γ/interleukin‐10 axis
Article Snippet: IL‐10 expression was knocked down in MSC using multiple human‐IL‐10‐specific siRNA sequences (Cat. No. sc‐39634; Santa Cruz Biotechnology, Santa Cruz, CA). .. MSCs were plated at a density of 5.6 × 104 MSC/cm2 24 hours before siRNA treatment. siRNA was added to a lipid‐based transfection agent (Santa Cruz) and allowed to incubate at room temperature for 20 minutes before being diluted in siRNA transfection medium according to the manufacturer's protocol (Santa Cruz). siRNA was added to cells at 150 pg/106 MSC for 6 hours. siRNA‐containing medium was then diluted 1:2 with complete medium and cells were incubated another 24 hours. .. Cultures were then aspirated and MSCs were either prepared for animal infusions (see above) or incubated in cell lysis buffer (Cell Signaling Technology, Danvers, MA) containing a protease inhibitor cocktail (Santa Cruz) for IL‐10 expression analysis by western blot (see below).

Expressing:

Article Title: Mesenchymal stromal cell potency to treat acute kidney injury increased by ultrasound‐activated interferon‐γ/interleukin‐10 axis. Mesenchymal stromal cell potency to treat acute kidney injury increased by ultrasound‐activated interferon‐γ/interleukin‐10 axis
Article Snippet: Groups of mice that were not treated with MSC did receive injections of sodium nitroprusside and saline as controls. .. 2.4 IL‐10 knockdown in MSC IL‐10 expression was knocked down in MSC using multiple human‐IL‐10‐specific siRNA sequences (Cat. No. sc‐39634; Santa Cruz Biotechnology, Santa Cruz, CA). .. MSCs were plated at a density of 5.6 × 104 MSC/cm2 24 hours before siRNA treatment. siRNA was added to a lipid‐based transfection agent (Santa Cruz) and allowed to incubate at room temperature for 20 minutes before being diluted in siRNA transfection medium according to the manufacturer's protocol (Santa Cruz). siRNA was added to cells at 150 pg/106 MSC for 6 hours. siRNA‐containing medium was then diluted 1:2 with complete medium and cells were incubated another 24 hours.

Article Title: Mesenchymal stromal cell potency to treat acute kidney injury increased by ultrasound‐activated interferon‐γ/interleukin‐10 axis. Mesenchymal stromal cell potency to treat acute kidney injury increased by ultrasound‐activated interferon‐γ/interleukin‐10 axis
Article Snippet: Groups of mice that were not treated with MSC did receive injections of sodium nitroprusside and saline as controls. .. IL‐10 expression was knocked down in MSC using multiple human‐IL‐10‐specific siRNA sequences (Cat. No. sc‐39634; Santa Cruz Biotechnology, Santa Cruz, CA). .. MSCs were plated at a density of 5.6 × 104 MSC/cm2 24 hours before siRNA treatment. siRNA was added to a lipid‐based transfection agent (Santa Cruz) and allowed to incubate at room temperature for 20 minutes before being diluted in siRNA transfection medium according to the manufacturer's protocol (Santa Cruz). siRNA was added to cells at 150 pg/106 MSC for 6 hours. siRNA‐containing medium was then diluted 1:2 with complete medium and cells were incubated another 24 hours.

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    Santa Cruz Biotechnology lipid based transfection agent
    Lipid Based Transfection Agent, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipid based transfection agent/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lipid based transfection agent - by Bioz Stars, 2021-07
    86/100 stars
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