Structured Review

Santa Cruz Biotechnology lipid based transfection agent
Lipid Based Transfection Agent, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lipid based transfection agent/product/Santa Cruz Biotechnology
Average 93 stars, based on 3 article reviews
Price from $9.99 to $1999.99
lipid based transfection agent - by Bioz Stars, 2020-07
93/100 stars

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Related Articles

Transfection:

Article Title: Mesenchymal stromal cell potency to treat acute kidney injury increased by ultrasound‐activated interferon‐γ/interleukin‐10 axis. Mesenchymal stromal cell potency to treat acute kidney injury increased by ultrasound‐activated interferon‐γ/interleukin‐10 axis
Article Snippet: .. MSCs were plated at a density of 5.6 × 104 MSC/cm2 24 hours before siRNA treatment. siRNA was added to a lipid‐based transfection agent (Santa Cruz) and allowed to incubate at room temperature for 20 minutes before being diluted in siRNA transfection medium according to the manufacturer's protocol (Santa Cruz). siRNA was added to cells at 150 pg/106 MSC for 6 hours. siRNA‐containing medium was then diluted 1:2 with complete medium and cells were incubated another 24 hours. .. Cultures were then aspirated and MSCs were either prepared for animal infusions (see above) or incubated in cell lysis buffer (Cell Signaling Technology, Danvers, MA) containing a protease inhibitor cocktail (Santa Cruz) for IL‐10 expression analysis by western blot (see below).

Incubation:

Article Title: Mesenchymal stromal cell potency to treat acute kidney injury increased by ultrasound‐activated interferon‐γ/interleukin‐10 axis. Mesenchymal stromal cell potency to treat acute kidney injury increased by ultrasound‐activated interferon‐γ/interleukin‐10 axis
Article Snippet: .. MSCs were plated at a density of 5.6 × 104 MSC/cm2 24 hours before siRNA treatment. siRNA was added to a lipid‐based transfection agent (Santa Cruz) and allowed to incubate at room temperature for 20 minutes before being diluted in siRNA transfection medium according to the manufacturer's protocol (Santa Cruz). siRNA was added to cells at 150 pg/106 MSC for 6 hours. siRNA‐containing medium was then diluted 1:2 with complete medium and cells were incubated another 24 hours. .. Cultures were then aspirated and MSCs were either prepared for animal infusions (see above) or incubated in cell lysis buffer (Cell Signaling Technology, Danvers, MA) containing a protease inhibitor cocktail (Santa Cruz) for IL‐10 expression analysis by western blot (see below).

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    Santa Cruz Biotechnology lipid based transfection agent
    Lipid Based Transfection Agent, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipid based transfection agent/product/Santa Cruz Biotechnology
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    lipid based transfection agent - by Bioz Stars, 2020-07
    93/100 stars
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