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Carl Zeiss light sheet fluorescence microscope imaging
3D rendering of an oblate ellipsoidal tumor. The 3D rendering, created using images taken with a <t>light</t> <t>sheet</t> <t>fluorescence</t> <t>microscope,</t> is rotated about the vertical axis in this sequence of images. Blue: Hoechst-stained nuclei; Red: E-cadherin. Scale bar is 90 µm.
Light Sheet Fluorescence Microscope Imaging, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/light sheet fluorescence microscope imaging/product/Carl Zeiss
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
light sheet fluorescence microscope imaging - by Bioz Stars, 2022-09
86/100 stars

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1) Product Images from "Elastic Free Energy Drives the Shape of Prevascular Solid Tumors"

Article Title: Elastic Free Energy Drives the Shape of Prevascular Solid Tumors

Journal: PLoS ONE

doi: 10.1371/journal.pone.0103245

3D rendering of an oblate ellipsoidal tumor. The 3D rendering, created using images taken with a light sheet fluorescence microscope, is rotated about the vertical axis in this sequence of images. Blue: Hoechst-stained nuclei; Red: E-cadherin. Scale bar is 90 µm.
Figure Legend Snippet: 3D rendering of an oblate ellipsoidal tumor. The 3D rendering, created using images taken with a light sheet fluorescence microscope, is rotated about the vertical axis in this sequence of images. Blue: Hoechst-stained nuclei; Red: E-cadherin. Scale bar is 90 µm.

Techniques Used: Fluorescence, Microscopy, Sequencing, Staining

The various projections of oblate ellipsoidal tumors. a. Projections (maximum intensity) of two oblate ellipsoidal grown in 0.5% agarose gel and imaged with a light sheet fluorescence microscope from four different directions. b . Rotational sequences of the 3D renderings. The 0° orientation is marked with an asterisk in a . Complete sequences are available as Movies S3 S4 . Fluorescence signal is from Hoechst-stained nuclei. Scale bars are all 50 µm.
Figure Legend Snippet: The various projections of oblate ellipsoidal tumors. a. Projections (maximum intensity) of two oblate ellipsoidal grown in 0.5% agarose gel and imaged with a light sheet fluorescence microscope from four different directions. b . Rotational sequences of the 3D renderings. The 0° orientation is marked with an asterisk in a . Complete sequences are available as Movies S3 S4 . Fluorescence signal is from Hoechst-stained nuclei. Scale bars are all 50 µm.

Techniques Used: Agarose Gel Electrophoresis, Fluorescence, Microscopy, Staining

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    Carl Zeiss light sheet fluorescence microscope imaging
    3D rendering of an oblate ellipsoidal tumor. The 3D rendering, created using images taken with a <t>light</t> <t>sheet</t> <t>fluorescence</t> <t>microscope,</t> is rotated about the vertical axis in this sequence of images. Blue: Hoechst-stained nuclei; Red: E-cadherin. Scale bar is 90 µm.
    Light Sheet Fluorescence Microscope Imaging, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/light sheet fluorescence microscope imaging/product/Carl Zeiss
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    light sheet fluorescence microscope imaging - by Bioz Stars, 2022-09
    90/100 stars
      Buy from Supplier

    90
    Carl Zeiss z1 light sheet fluorescence microscopy lsfm
    Overview of experimental pipeline for Octopus vulgaris embryos. RNA in situ hybridization chain reaction version 3.0 (RNA-ISH) and immunohistochemistry (IHC) are followed by fructose-glycerol clearing and imaging with <t>Light</t> <t>Sheet</t> <t>Fluorescence</t> <t>Microscopy</t> <t>(LSFM).</t> The final images (3D images and Z-stack planes) as well as videos are acquired, processed and analyzed with ZEN (black edition) and ARIVIS VISION4D v.3.1.4 software. For developmental stage XV embryo (its size is approximately 1,25 mm x 0,88 mm), RNA-ISH Clearing Imaging Image Analysis takes approximately 7 days whereas, RNA-ISH IHC Clearing Imaging Image Analysis takes around 9 days. (This figure is designed using a resource from freepik.com ).
    Z1 Light Sheet Fluorescence Microscopy Lsfm, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/z1 light sheet fluorescence microscopy lsfm/product/Carl Zeiss
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    z1 light sheet fluorescence microscopy lsfm - by Bioz Stars, 2022-09
    90/100 stars
      Buy from Supplier

    88
    Carl Zeiss light sheet fluorescence microscope
    Localisation of CD133 and CD44 stem cell markers within recurrent gliomaspheres. ( A , B ) <t>Light-sheet</t> <t>fluorescence</t> microscopy was used to assess the localisation of CD133 and CD44 stem cell biomarkers in GTCC-9 ( A ) and GTCC-10 ( B ) gliomaspheres; representative images are shown here; scale bar = 50 μm ( A ); scale bar = 100 μm ( B ); steps between optical slices indicated in µm in ( A , B ); n = 3 independent experiments performed.
    Light Sheet Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/light sheet fluorescence microscope/product/Carl Zeiss
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    light sheet fluorescence microscope - by Bioz Stars, 2022-09
    88/100 stars
      Buy from Supplier

    Image Search Results


    3D rendering of an oblate ellipsoidal tumor. The 3D rendering, created using images taken with a light sheet fluorescence microscope, is rotated about the vertical axis in this sequence of images. Blue: Hoechst-stained nuclei; Red: E-cadherin. Scale bar is 90 µm.

    Journal: PLoS ONE

    Article Title: Elastic Free Energy Drives the Shape of Prevascular Solid Tumors

    doi: 10.1371/journal.pone.0103245

    Figure Lengend Snippet: 3D rendering of an oblate ellipsoidal tumor. The 3D rendering, created using images taken with a light sheet fluorescence microscope, is rotated about the vertical axis in this sequence of images. Blue: Hoechst-stained nuclei; Red: E-cadherin. Scale bar is 90 µm.

    Article Snippet: Light sheet fluorescence microscope imaging (for ) was performed on a Zeiss Lightsheet Z.1 microscope with the help of Dr. C. Schwindling at the Zeiss Microscopy Labs in Munich, Germany and (for ) on a custom-built light sheet fluorescence microscope in the lab of Prof. H. Schneckenburger with the assistance of S. Schickinger at the University of Aalen, Germany .

    Techniques: Fluorescence, Microscopy, Sequencing, Staining

    The various projections of oblate ellipsoidal tumors. a. Projections (maximum intensity) of two oblate ellipsoidal grown in 0.5% agarose gel and imaged with a light sheet fluorescence microscope from four different directions. b . Rotational sequences of the 3D renderings. The 0° orientation is marked with an asterisk in a . Complete sequences are available as Movies S3 S4 . Fluorescence signal is from Hoechst-stained nuclei. Scale bars are all 50 µm.

    Journal: PLoS ONE

    Article Title: Elastic Free Energy Drives the Shape of Prevascular Solid Tumors

    doi: 10.1371/journal.pone.0103245

    Figure Lengend Snippet: The various projections of oblate ellipsoidal tumors. a. Projections (maximum intensity) of two oblate ellipsoidal grown in 0.5% agarose gel and imaged with a light sheet fluorescence microscope from four different directions. b . Rotational sequences of the 3D renderings. The 0° orientation is marked with an asterisk in a . Complete sequences are available as Movies S3 S4 . Fluorescence signal is from Hoechst-stained nuclei. Scale bars are all 50 µm.

    Article Snippet: Light sheet fluorescence microscope imaging (for ) was performed on a Zeiss Lightsheet Z.1 microscope with the help of Dr. C. Schwindling at the Zeiss Microscopy Labs in Munich, Germany and (for ) on a custom-built light sheet fluorescence microscope in the lab of Prof. H. Schneckenburger with the assistance of S. Schickinger at the University of Aalen, Germany .

    Techniques: Agarose Gel Electrophoresis, Fluorescence, Microscopy, Staining

    Overview of experimental pipeline for Octopus vulgaris embryos. RNA in situ hybridization chain reaction version 3.0 (RNA-ISH) and immunohistochemistry (IHC) are followed by fructose-glycerol clearing and imaging with Light Sheet Fluorescence Microscopy (LSFM). The final images (3D images and Z-stack planes) as well as videos are acquired, processed and analyzed with ZEN (black edition) and ARIVIS VISION4D v.3.1.4 software. For developmental stage XV embryo (its size is approximately 1,25 mm x 0,88 mm), RNA-ISH Clearing Imaging Image Analysis takes approximately 7 days whereas, RNA-ISH IHC Clearing Imaging Image Analysis takes around 9 days. (This figure is designed using a resource from freepik.com ).

    Journal: Frontiers in Physiology

    Article Title: Optimization of Whole Mount RNA Multiplexed in situ Hybridization Chain Reaction With Immunohistochemistry, Clearing and Imaging to Visualize Octopus Embryonic Neurogenesis

    doi: 10.3389/fphys.2022.882413

    Figure Lengend Snippet: Overview of experimental pipeline for Octopus vulgaris embryos. RNA in situ hybridization chain reaction version 3.0 (RNA-ISH) and immunohistochemistry (IHC) are followed by fructose-glycerol clearing and imaging with Light Sheet Fluorescence Microscopy (LSFM). The final images (3D images and Z-stack planes) as well as videos are acquired, processed and analyzed with ZEN (black edition) and ARIVIS VISION4D v.3.1.4 software. For developmental stage XV embryo (its size is approximately 1,25 mm x 0,88 mm), RNA-ISH Clearing Imaging Image Analysis takes approximately 7 days whereas, RNA-ISH IHC Clearing Imaging Image Analysis takes around 9 days. (This figure is designed using a resource from freepik.com ).

    Article Snippet: Imaging was done using Zeiss Z1 Light sheet fluorescence microscopy (LSFM) (Carl Zeiss AG, Germany).

    Techniques: RNA In Situ Hybridization, In Situ Hybridization, Immunohistochemistry, Imaging, Fluorescence, Microscopy, Software

    Whole Mount HCR v3.0 followed by fructose-glycerol clearing on an Octopus vulgaris embryo (developmental stage XV) imaged with LSFM. Top panel illustrates the merged 3D view from the posterior side of the embryo, and bottom panel shows a single plane of a coronal section. (A) Overview image showing the expression of Ov-elav and Ov-apolpp on a Stage XV embryo in 3D. Note that only high-level expression is retained on the merged view. DAPI (in grey) is used for nuclear labelling. (B–D) 3 individual channels from (A) . (E) Overview image showing the expression of Ov-elav and Ov-apolpp on a coronal section of Stage XV embryo. (F–H) 3 individual channels from (E) . Abbreviations: ar, arm; D, dorsal; ey, eye; fu, funnel; gg, gastric ganglion; LL, lateral lip; ma, mantle; n, neuropil; OL, optic lobe; SEM, supraesophageal mass; sg, stellate ganglion;SUB, subesophageal mass; V, ventral; y, yolk.

    Journal: Frontiers in Physiology

    Article Title: Optimization of Whole Mount RNA Multiplexed in situ Hybridization Chain Reaction With Immunohistochemistry, Clearing and Imaging to Visualize Octopus Embryonic Neurogenesis

    doi: 10.3389/fphys.2022.882413

    Figure Lengend Snippet: Whole Mount HCR v3.0 followed by fructose-glycerol clearing on an Octopus vulgaris embryo (developmental stage XV) imaged with LSFM. Top panel illustrates the merged 3D view from the posterior side of the embryo, and bottom panel shows a single plane of a coronal section. (A) Overview image showing the expression of Ov-elav and Ov-apolpp on a Stage XV embryo in 3D. Note that only high-level expression is retained on the merged view. DAPI (in grey) is used for nuclear labelling. (B–D) 3 individual channels from (A) . (E) Overview image showing the expression of Ov-elav and Ov-apolpp on a coronal section of Stage XV embryo. (F–H) 3 individual channels from (E) . Abbreviations: ar, arm; D, dorsal; ey, eye; fu, funnel; gg, gastric ganglion; LL, lateral lip; ma, mantle; n, neuropil; OL, optic lobe; SEM, supraesophageal mass; sg, stellate ganglion;SUB, subesophageal mass; V, ventral; y, yolk.

    Article Snippet: Imaging was done using Zeiss Z1 Light sheet fluorescence microscopy (LSFM) (Carl Zeiss AG, Germany).

    Techniques: Expressing

    Whole Mount multiplexed HCR v3.0-IHC followed by fructose-glycerol clearing on an Octopus vulgaris embryo (developmental stage XV) imaged with LSFM to visualize neurogenesis. (A) Overview image showing the expression of Ov-ascl1 and Ov-neuroD and presence of mitotic cells (PH3+) on a Stage XV embryo in 3D view. DAPI (in grey) is used for nuclear labelling. (B) Image illustrating mitotic PH3+ cells with DAPI which is an indication of successful IHC after HCR. (C) Multiplexed HCR Image of Ov-ascl1 and Ov-neuroD with DAPI. (D–G) Separate channels from (A) . (H) Overlay of Ov-ascl1 and Ov-neuroD show mutually exclusive expression. Yellow line indicates the transition zone area. (I) Overview image showing the expression of Ov-ascl1 and Ov-neuroD and presence of mitotic cells (PH3+) on a coronal section of Stage XV embryo. (J–M) 4 individual channels from (I) . Abbreviations: ar, arm; D, dorsal; ey, eye; fu, funnel; LL, lateral lip; ma, mantle; OL, optic lobe; SUB, subesophageal mass; V: ventral; y, yolk.

    Journal: Frontiers in Physiology

    Article Title: Optimization of Whole Mount RNA Multiplexed in situ Hybridization Chain Reaction With Immunohistochemistry, Clearing and Imaging to Visualize Octopus Embryonic Neurogenesis

    doi: 10.3389/fphys.2022.882413

    Figure Lengend Snippet: Whole Mount multiplexed HCR v3.0-IHC followed by fructose-glycerol clearing on an Octopus vulgaris embryo (developmental stage XV) imaged with LSFM to visualize neurogenesis. (A) Overview image showing the expression of Ov-ascl1 and Ov-neuroD and presence of mitotic cells (PH3+) on a Stage XV embryo in 3D view. DAPI (in grey) is used for nuclear labelling. (B) Image illustrating mitotic PH3+ cells with DAPI which is an indication of successful IHC after HCR. (C) Multiplexed HCR Image of Ov-ascl1 and Ov-neuroD with DAPI. (D–G) Separate channels from (A) . (H) Overlay of Ov-ascl1 and Ov-neuroD show mutually exclusive expression. Yellow line indicates the transition zone area. (I) Overview image showing the expression of Ov-ascl1 and Ov-neuroD and presence of mitotic cells (PH3+) on a coronal section of Stage XV embryo. (J–M) 4 individual channels from (I) . Abbreviations: ar, arm; D, dorsal; ey, eye; fu, funnel; LL, lateral lip; ma, mantle; OL, optic lobe; SUB, subesophageal mass; V: ventral; y, yolk.

    Article Snippet: Imaging was done using Zeiss Z1 Light sheet fluorescence microscopy (LSFM) (Carl Zeiss AG, Germany).

    Techniques: Immunohistochemistry, Expressing

    Localisation of CD133 and CD44 stem cell markers within recurrent gliomaspheres. ( A , B ) Light-sheet fluorescence microscopy was used to assess the localisation of CD133 and CD44 stem cell biomarkers in GTCC-9 ( A ) and GTCC-10 ( B ) gliomaspheres; representative images are shown here; scale bar = 50 μm ( A ); scale bar = 100 μm ( B ); steps between optical slices indicated in µm in ( A , B ); n = 3 independent experiments performed.

    Journal: Cells

    Article Title: Transcriptional CDK Inhibitors CYC065 and THZ1 Induce Apoptosis in Glioma Stem Cells Derived from Recurrent GBM

    doi: 10.3390/cells10051182

    Figure Lengend Snippet: Localisation of CD133 and CD44 stem cell markers within recurrent gliomaspheres. ( A , B ) Light-sheet fluorescence microscopy was used to assess the localisation of CD133 and CD44 stem cell biomarkers in GTCC-9 ( A ) and GTCC-10 ( B ) gliomaspheres; representative images are shown here; scale bar = 50 μm ( A ); scale bar = 100 μm ( B ); steps between optical slices indicated in µm in ( A , B ); n = 3 independent experiments performed.

    Article Snippet: For imaging the capillary was mounted in the microscope sample holder and the chamber of the Light Sheet Fluorescence Microscope (Lightsheet Z1, Carl Zeiss, Germany).

    Techniques: Fluorescence, Microscopy

    PAFhy-3D imaging for other diseases. ( A ) Reconstructed 3D image of whole colonic polyp obtained by light-sheet fluorescence microscopy. An optical slice image derived from this 3D model is also shown. This polyp was subjected to generation of H E- and PAS-stained glass slides after PAFhy-3D imaging and subsequent phosphate-buffered saline washes. H E- and PAS-stained histopathological images show that the polyp consists of tubular adenoma. Scale bar, 1 mm (3D model) or 200 μm (optical slice, H E, PAS). ( B ) PAFhy-3D imaging of a human colonic polyp by light-sheet fluorescence microscopy combined with vascular structure staining by a fluorescence-conjugated lectin. A colonic polyp was cleared with CUBIC reagents and stained with FAM hydrazide, propidium iodide (PI), and DyLight 649-conjugated Lycopersicon esculentum (Tomato) lectin and then imaged by light-sheet fluorescence microscopy. Only this 3D image underwent imaging processing with a normalized filer. 36 The images of individual channels (Ch-PAFhy with an excitation wavelength at 488 nm, Ch-PI at 592 nm, and Ch-Tomato lectin-Dy649 at 642 nm) were independently downscaled into 1/15.4 (from 2048 × 2048 to 133 × 133 pixel images). These images were subjected to image processing with a normalized filer. Subsequently, a mask image (pixels of > 200 signal intensity were regarded as positive) was generated from the Ch-PAFhy image. This mask image was applied to images of Ch-PI and Ch-Tomato lectin-DyLight 649. Scale bar, 1 mm. ( C ) Reconstructed 3D image of lung tissue with pulmonary aspergillosis. H E- and PAS-stained images from glass slides generated from the same lesion and optical-slice images of orthographic projection derived from the 3D model are also shown. Scale bar, 100 μm.

    Journal: Cellular and Molecular Gastroenterology and Hepatology

    Article Title: A Novel Three-Dimensional Imaging System Based on Polysaccharide Staining for Accurate Histopathological Diagnosis of Inflammatory Bowel Diseases

    doi: 10.1016/j.jcmgh.2022.07.001

    Figure Lengend Snippet: PAFhy-3D imaging for other diseases. ( A ) Reconstructed 3D image of whole colonic polyp obtained by light-sheet fluorescence microscopy. An optical slice image derived from this 3D model is also shown. This polyp was subjected to generation of H E- and PAS-stained glass slides after PAFhy-3D imaging and subsequent phosphate-buffered saline washes. H E- and PAS-stained histopathological images show that the polyp consists of tubular adenoma. Scale bar, 1 mm (3D model) or 200 μm (optical slice, H E, PAS). ( B ) PAFhy-3D imaging of a human colonic polyp by light-sheet fluorescence microscopy combined with vascular structure staining by a fluorescence-conjugated lectin. A colonic polyp was cleared with CUBIC reagents and stained with FAM hydrazide, propidium iodide (PI), and DyLight 649-conjugated Lycopersicon esculentum (Tomato) lectin and then imaged by light-sheet fluorescence microscopy. Only this 3D image underwent imaging processing with a normalized filer. 36 The images of individual channels (Ch-PAFhy with an excitation wavelength at 488 nm, Ch-PI at 592 nm, and Ch-Tomato lectin-Dy649 at 642 nm) were independently downscaled into 1/15.4 (from 2048 × 2048 to 133 × 133 pixel images). These images were subjected to image processing with a normalized filer. Subsequently, a mask image (pixels of > 200 signal intensity were regarded as positive) was generated from the Ch-PAFhy image. This mask image was applied to images of Ch-PI and Ch-Tomato lectin-DyLight 649. Scale bar, 1 mm. ( C ) Reconstructed 3D image of lung tissue with pulmonary aspergillosis. H E- and PAS-stained images from glass slides generated from the same lesion and optical-slice images of orthographic projection derived from the 3D model are also shown. Scale bar, 100 μm.

    Article Snippet: A 3D image of a colonic polyp shown in A was acquired using a light-sheet fluorescence microscope (Lightsheet 7; Carl Zeiss).

    Techniques: Imaging, Fluorescence, Microscopy, Derivative Assay, Staining, Generated