ligation mix  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    New England Biolabs ligation mix
    Ligation Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ligation mix/product/New England Biolabs
    Average 99 stars, based on 70 article reviews
    Price from $9.99 to $1999.99
    ligation mix - by Bioz Stars, 2020-02
    99/100 stars

    Images

    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: Clinical and molecular study of a pediatric patient with sodium taurocholate cotransporting polypeptide deficiency
    Article Snippet: Following quality-control measurements, the DNA was randomly fragmented into sections of 200–300 base pairs by using a ultrasonicator (Covaris, Inc., Woburn, MA, USA), then fragments of the desired length were purified and separated using gel electrophoresis in a 2% agarose gel, purified with a QIAquick PCR Purification Kit (QIAGEN, Suzhou, Jiangsu, China), added into End Repair Mix (New England Biolabs, Inc., Ipswich, MA, USA), and incubated at 20°C for 30 min. .. The end-repaired DNA was purified with a QIAquick PCR Purification Kit (QIAGEN), then added to an A-Tailing Mix (New England Biolabs, Inc.), and incubated at 37°C for 30 min. Then, a ligation reaction was conducted by incubating the purified Adenylate 3′ Ends DNA, Adapter and Ligation Mix (New England Biolabs, Inc.) at 20°C for 15 min. Adapter-ligated DNA was selected by running a 2% agarose gel and purified with a QIAquick Gel Extraction kit (QIAGEN).

    Amplification:

    Article Title: Clinical and molecular study of a pediatric patient with sodium taurocholate cotransporting polypeptide deficiency
    Article Snippet: The end-repaired DNA was purified with a QIAquick PCR Purification Kit (QIAGEN), then added to an A-Tailing Mix (New England Biolabs, Inc.), and incubated at 37°C for 30 min. Then, a ligation reaction was conducted by incubating the purified Adenylate 3′ Ends DNA, Adapter and Ligation Mix (New England Biolabs, Inc.) at 20°C for 15 min. Adapter-ligated DNA was selected by running a 2% agarose gel and purified with a QIAquick Gel Extraction kit (QIAGEN). .. Following that, several rounds of PCR amplification were performed using a PCR Primer Cocktail and PCR Master Mix (New England Biolabs, Inc.) to enrich the Adapter-ligated DNA fragments.

    Agarose Gel Electrophoresis:

    Article Title: Clinical and molecular study of a pediatric patient with sodium taurocholate cotransporting polypeptide deficiency
    Article Snippet: .. The end-repaired DNA was purified with a QIAquick PCR Purification Kit (QIAGEN), then added to an A-Tailing Mix (New England Biolabs, Inc.), and incubated at 37°C for 30 min. Then, a ligation reaction was conducted by incubating the purified Adenylate 3′ Ends DNA, Adapter and Ligation Mix (New England Biolabs, Inc.) at 20°C for 15 min. Adapter-ligated DNA was selected by running a 2% agarose gel and purified with a QIAquick Gel Extraction kit (QIAGEN). .. Following that, several rounds of PCR amplification were performed using a PCR Primer Cocktail and PCR Master Mix (New England Biolabs, Inc.) to enrich the Adapter-ligated DNA fragments.

    Ligation:

    Article Title: Clinical and molecular study of a pediatric patient with sodium taurocholate cotransporting polypeptide deficiency
    Article Snippet: .. The end-repaired DNA was purified with a QIAquick PCR Purification Kit (QIAGEN), then added to an A-Tailing Mix (New England Biolabs, Inc.), and incubated at 37°C for 30 min. Then, a ligation reaction was conducted by incubating the purified Adenylate 3′ Ends DNA, Adapter and Ligation Mix (New England Biolabs, Inc.) at 20°C for 15 min. Adapter-ligated DNA was selected by running a 2% agarose gel and purified with a QIAquick Gel Extraction kit (QIAGEN). .. Following that, several rounds of PCR amplification were performed using a PCR Primer Cocktail and PCR Master Mix (New England Biolabs, Inc.) to enrich the Adapter-ligated DNA fragments.

    Next-Generation Sequencing:

    Article Title: Clinical and molecular study of a pediatric patient with sodium taurocholate cotransporting polypeptide deficiency
    Article Snippet: Whole genome sequencing To eliminate the possibility of other genetic causes resulting in hypercholanemia in the patient, whole genome sequencing was performed using next generation sequencing (NGS). .. The end-repaired DNA was purified with a QIAquick PCR Purification Kit (QIAGEN), then added to an A-Tailing Mix (New England Biolabs, Inc.), and incubated at 37°C for 30 min. Then, a ligation reaction was conducted by incubating the purified Adenylate 3′ Ends DNA, Adapter and Ligation Mix (New England Biolabs, Inc.) at 20°C for 15 min. Adapter-ligated DNA was selected by running a 2% agarose gel and purified with a QIAquick Gel Extraction kit (QIAGEN).

    Spectrophotometry:

    Article Title: Clinical and molecular study of a pediatric patient with sodium taurocholate cotransporting polypeptide deficiency
    Article Snippet: For quality-control, DNA concentrations were tested with a Nanodrop Spectrophotometer (Thermo Fisher Scientific, Inc.). .. The end-repaired DNA was purified with a QIAquick PCR Purification Kit (QIAGEN), then added to an A-Tailing Mix (New England Biolabs, Inc.), and incubated at 37°C for 30 min. Then, a ligation reaction was conducted by incubating the purified Adenylate 3′ Ends DNA, Adapter and Ligation Mix (New England Biolabs, Inc.) at 20°C for 15 min. Adapter-ligated DNA was selected by running a 2% agarose gel and purified with a QIAquick Gel Extraction kit (QIAGEN).

    Purification:

    Article Title: Clinical and molecular study of a pediatric patient with sodium taurocholate cotransporting polypeptide deficiency
    Article Snippet: .. The end-repaired DNA was purified with a QIAquick PCR Purification Kit (QIAGEN), then added to an A-Tailing Mix (New England Biolabs, Inc.), and incubated at 37°C for 30 min. Then, a ligation reaction was conducted by incubating the purified Adenylate 3′ Ends DNA, Adapter and Ligation Mix (New England Biolabs, Inc.) at 20°C for 15 min. Adapter-ligated DNA was selected by running a 2% agarose gel and purified with a QIAquick Gel Extraction kit (QIAGEN). .. Following that, several rounds of PCR amplification were performed using a PCR Primer Cocktail and PCR Master Mix (New England Biolabs, Inc.) to enrich the Adapter-ligated DNA fragments.

    Polymerase Chain Reaction:

    Article Title: Clinical and molecular study of a pediatric patient with sodium taurocholate cotransporting polypeptide deficiency
    Article Snippet: .. The end-repaired DNA was purified with a QIAquick PCR Purification Kit (QIAGEN), then added to an A-Tailing Mix (New England Biolabs, Inc.), and incubated at 37°C for 30 min. Then, a ligation reaction was conducted by incubating the purified Adenylate 3′ Ends DNA, Adapter and Ligation Mix (New England Biolabs, Inc.) at 20°C for 15 min. Adapter-ligated DNA was selected by running a 2% agarose gel and purified with a QIAquick Gel Extraction kit (QIAGEN). .. Following that, several rounds of PCR amplification were performed using a PCR Primer Cocktail and PCR Master Mix (New England Biolabs, Inc.) to enrich the Adapter-ligated DNA fragments.

    Incubation:

    Article Title: Clinical and molecular study of a pediatric patient with sodium taurocholate cotransporting polypeptide deficiency
    Article Snippet: .. The end-repaired DNA was purified with a QIAquick PCR Purification Kit (QIAGEN), then added to an A-Tailing Mix (New England Biolabs, Inc.), and incubated at 37°C for 30 min. Then, a ligation reaction was conducted by incubating the purified Adenylate 3′ Ends DNA, Adapter and Ligation Mix (New England Biolabs, Inc.) at 20°C for 15 min. Adapter-ligated DNA was selected by running a 2% agarose gel and purified with a QIAquick Gel Extraction kit (QIAGEN). .. Following that, several rounds of PCR amplification were performed using a PCR Primer Cocktail and PCR Master Mix (New England Biolabs, Inc.) to enrich the Adapter-ligated DNA fragments.

    Sequencing:

    Article Title: Clinical and molecular study of a pediatric patient with sodium taurocholate cotransporting polypeptide deficiency
    Article Snippet: Paragraph title: Whole genome sequencing ... The end-repaired DNA was purified with a QIAquick PCR Purification Kit (QIAGEN), then added to an A-Tailing Mix (New England Biolabs, Inc.), and incubated at 37°C for 30 min. Then, a ligation reaction was conducted by incubating the purified Adenylate 3′ Ends DNA, Adapter and Ligation Mix (New England Biolabs, Inc.) at 20°C for 15 min. Adapter-ligated DNA was selected by running a 2% agarose gel and purified with a QIAquick Gel Extraction kit (QIAGEN).

    Gel Extraction:

    Article Title: Clinical and molecular study of a pediatric patient with sodium taurocholate cotransporting polypeptide deficiency
    Article Snippet: .. The end-repaired DNA was purified with a QIAquick PCR Purification Kit (QIAGEN), then added to an A-Tailing Mix (New England Biolabs, Inc.), and incubated at 37°C for 30 min. Then, a ligation reaction was conducted by incubating the purified Adenylate 3′ Ends DNA, Adapter and Ligation Mix (New England Biolabs, Inc.) at 20°C for 15 min. Adapter-ligated DNA was selected by running a 2% agarose gel and purified with a QIAquick Gel Extraction kit (QIAGEN). .. Following that, several rounds of PCR amplification were performed using a PCR Primer Cocktail and PCR Master Mix (New England Biolabs, Inc.) to enrich the Adapter-ligated DNA fragments.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 89
    New England Biolabs ligation reaction mixture b
    Ligation Reaction Mixture B, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 89/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ligation reaction mixture b/product/New England Biolabs
    Average 89 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    ligation reaction mixture b - by Bioz Stars, 2020-02
    89/100 stars
      Buy from Supplier

    99
    New England Biolabs ligation master mix
    Ligation Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ligation master mix/product/New England Biolabs
    Average 99 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    ligation master mix - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    Image Search Results