Structured Review

Upstate Biotechnology Inc licl immune complex wash buffer
Licl Immune Complex Wash Buffer, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/licl immune complex wash buffer/product/Upstate Biotechnology Inc
Average 88 stars, based on 2 article reviews
Price from $9.99 to $1999.99
licl immune complex wash buffer - by Bioz Stars, 2020-07
88/100 stars

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Incubation:

Article Title: Role of Glucocorticoids and cAMP-Mediated Repression in Limiting Corticotropin-Releasing Hormone Transcription during Stress
Article Snippet: .. After overnight incubation, 65 μl of salmon-sperm DNA/protein A-agarose slurry was added and incubated for 1 h at 4°C under rotation, and DNA-protein complexes were separated by centrifugation at 100 × g for 3 min. Pellets were washed sequentially in low-salt immune-complex wash buffer, high-salt immune-complex wash buffer, and LiCl immune-complex wash buffer (Upstate Biotechnology) for 3-5 min, followed by two washes in Tris-EDTA buffer and elution twice with 250 μl of 1% SDS in 0.1 m NaHCO3 . .. Aliquots of eluate pools and input chromatin were incubated at 65°C for 4-18 h in 0.2 m NaCl to reverse cross-linking and then incubated at 45°C for 1 h in 40 μ m Tris-HCl, pH 6.5, containing 10 μ m EDTA and 20 μg of proteinase K to remove proteins.

Centrifugation:

Article Title: Role of Glucocorticoids and cAMP-Mediated Repression in Limiting Corticotropin-Releasing Hormone Transcription during Stress
Article Snippet: .. After overnight incubation, 65 μl of salmon-sperm DNA/protein A-agarose slurry was added and incubated for 1 h at 4°C under rotation, and DNA-protein complexes were separated by centrifugation at 100 × g for 3 min. Pellets were washed sequentially in low-salt immune-complex wash buffer, high-salt immune-complex wash buffer, and LiCl immune-complex wash buffer (Upstate Biotechnology) for 3-5 min, followed by two washes in Tris-EDTA buffer and elution twice with 250 μl of 1% SDS in 0.1 m NaHCO3 . .. Aliquots of eluate pools and input chromatin were incubated at 65°C for 4-18 h in 0.2 m NaCl to reverse cross-linking and then incubated at 45°C for 1 h in 40 μ m Tris-HCl, pH 6.5, containing 10 μ m EDTA and 20 μg of proteinase K to remove proteins.

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    Upstate Biotechnology Inc licl immune complex wash buffer
    Licl Immune Complex Wash Buffer, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/licl immune complex wash buffer/product/Upstate Biotechnology Inc
    Average 88 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    licl immune complex wash buffer - by Bioz Stars, 2020-07
    88/100 stars
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