liberase blendzyme 4 solution  (Roche)


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    Roche liberase blendzyme 4 solution
    Liberase Blendzyme 4 Solution, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/liberase blendzyme 4 solution/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    liberase blendzyme 4 solution - by Bioz Stars, 2021-04
    86/100 stars

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    Article Title: Cancer initiating-cells are enriched in the CA9 positive fraction of primary cervix cancer xenografts
    Article Snippet: Enzymatic dissociation of pooled OCICx or ME180 xenografts was performed with a solution of 1X Media 199 (Invitrogen) with a mix of Liberase Blendzymes 2 & 4 (Roche) or Liberase TH (Roche) and DNAse I (Sigma).

    Article Title: SSEA-5, an antibody defining a novel surface glycan on human pluripotent stem cells and its application to remove teratoma-forming cells as part of a surface antibody panel
    Article Snippet: To dissociate ovarian cancers and hESC-derived teratomas, tumors were minced and then placed in a solution of Liberase Blendzymes 2 and 4 (Roche) in Media 199 (Invitrogen).

    Article Title: Sex differences in repolarization and slow delayed rectifier potassium current and their regulation by sympathetic stimulation in rabbits
    Article Snippet: The heart was then perfused with a MEM solution containing Liberase Blendzyme 4 (Roche Applied Science).

    Article Title: Nitroxyl enhances myocyte Ca2+ transients by exclusively targeting SR Ca2+-cycling
    Article Snippet: Perfusion was then switched to the same solution, but now containing Liberase Blendzyme 4 (Roche Diagnostics, Indianapolis, IN).

    Article Title: Protein Kinase C? Is Required for Autophagy in Response to Stress in the Endoplasmic Reticulum *Protein Kinase C? Is Required for Autophagy in Response to Stress in the Endoplasmic Reticulum * S⃞
    Article Snippet: The liver mass was permitted to settle to replace the trypsin solution with 0.05 mg/ml Liberase Blendzyme 4 solution (Roche Applied Science).

    Article Title: Dedifferentiation and Proliferation of Mammalian Cardiomyocytes
    Article Snippet: Briefly, mouse hearts were perfused similarly as for the rat myocyte protocol, with Ca2+ -free Tyrode's solution for 2 min, followed by digestion for 6–8 min in Ca2+ -free Tyrode's solution containing 0.15 Wünsch unit/mL of collagenase made from Liberase Blendzyme 4, followed by washing in KB solution for 5 min.

    Modification:

    Article Title: Akt2 ablation prolongs life span and improves myocardial contractile function with adaptive cardiac remodeling: role of Sirt1‐mediated autophagy regulation
    Article Snippet: Isolation of mouse cardiomyocytes Hearts were removed following anesthesia (ketamine 80 mg kg−1 and xylazine 12 mg kg−1 i.p.) and were mounted onto a temperature‐controlled (37 °C) Langendorff system. .. Following perfusion with a modified Tyrode solution (Ca2+ free) for 2 min, hearts were digested with a Ca2+ ‐free KHB buffer containing liberase blendzyme 4 (Hoffmann‐La Roche Inc., Indianapolis, IN, USA) for 20 min. .. The modified Tyrode solution (pH 7.4) contained the following (in mm ): NaCl 135, KCl 4.0, MgCl2 1.0, HEPES 10, NaH2 PO4 0.33, glucose 10, butanedione monoxime 10, and the solution was gassed with 5% CO2 –95% O2 .

    Article Title: Mitochondrial ALDH2 protects against lipopolysaccharide-induced myocardial contractile dysfunction by suppression of ER stress and autophagy.
    Article Snippet: 2.2: Isolation of cardiomyocytes and in vitro drug treatment: After ketamine sedation, hearts were rapidly removed and mounted onto a temperature-controlled (37°C) Langendorff system. .. The hearts were digested for 10–15 min with a modified Tyrode solution containing Liberase Blendzyme 4 (Hoffmann-La Roche Inc., Indianapolis, IN). .. The modified Tyrode solution (pH 7.4) contained the following (in mM): NaCl 135, KCl 4.0, MgCl2 1.0, HEPES ACCEPTED MANUSCRIPT AC C EP TE D M AN U SC R IP T 7 10, NaH2PO4 0.33, glucose 10, and butanedione monoxime 10, and the solution was gassed with 5% CO2-95% O2.

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    Roche liberase blendzyme 4
    Liberase Blendzyme 4, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/liberase blendzyme 4/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    liberase blendzyme 4 - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Roche liberase blendzyme 4 solution
    Liberase Blendzyme 4 Solution, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/liberase blendzyme 4 solution/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    liberase blendzyme 4 solution - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Roche collagenase solution
    Collagenase Solution, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagenase solution/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    collagenase solution - by Bioz Stars, 2021-04
    86/100 stars
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    86
    Roche liberase blendzyme 3
    Liberase Blendzyme 3, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/liberase blendzyme 3/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    liberase blendzyme 3 - by Bioz Stars, 2021-04
    86/100 stars
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