Structured Review

ICN Biomedicals leupeptin
Leupeptin, supplied by ICN Biomedicals, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/leupeptin/product/ICN Biomedicals
Average 92 stars, based on 11 article reviews
Price from $9.99 to $1999.99
leupeptin - by Bioz Stars, 2020-07
92/100 stars

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Related Articles

Western Blot:

Article Title: Crizotinib Exhibits Antitumor Activity by Targeting ALK Signaling not c-MET in Pancreatic Cancer
Article Snippet: .. Western blotting Total cellular proteins were extracted with a lysis buffer containing 1% Igepal CA-630, 20 mM Tris-HCl (pH 8.0), 137 μM NaCl, 10% glycerol, and 2 μM EDTA, as well as the following protease and phophatase inhibitor: aprotinin (10 mg/ml), leupeptin (10 mg/ml; ICN Biomedicals, Irvine, California), phenylmethylsulfonyl fluoride (1.72 μM), NaF (100 μM), NaVO3 (500 μM), and Na4 P2 O7 (500 mg/mL; Sigma-Aldrich). .. The proteins were separated by a sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE), which were then transferred onto nitrocellulose membranes.

Article Title: P-cadherin counteracts myosin II-B function: implications in melanoma progression
Article Snippet: .. Polyacrylamide gel electrophoresis (PAGE), Western blotting and immunoprecipitation experiments Cells were lysed using PBS containing 1% Triton X-100, 1% NP40 (Sigma, MO, US) and the following protease inhibitors: Leupeptin (10 μg/mL), aprotinin (10 μg/mL) (ICN Biomedicals, CA, US), phenylmethylsulfonyl fluoride (1.72 mM), NaF (100 μM), NaVO3 (500 μM), and Na4P2O7 (500 μg/mL) (Sigma, MO, US). .. After preparing lysates, protein concentration was measured using Rc-Dc protein assay kit (Bio-Rad Laboratories S.A.-N.V, Belgium).

Lysis:

Article Title: Crizotinib Exhibits Antitumor Activity by Targeting ALK Signaling not c-MET in Pancreatic Cancer
Article Snippet: .. Western blotting Total cellular proteins were extracted with a lysis buffer containing 1% Igepal CA-630, 20 mM Tris-HCl (pH 8.0), 137 μM NaCl, 10% glycerol, and 2 μM EDTA, as well as the following protease and phophatase inhibitor: aprotinin (10 mg/ml), leupeptin (10 mg/ml; ICN Biomedicals, Irvine, California), phenylmethylsulfonyl fluoride (1.72 μM), NaF (100 μM), NaVO3 (500 μM), and Na4 P2 O7 (500 mg/mL; Sigma-Aldrich). .. The proteins were separated by a sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE), which were then transferred onto nitrocellulose membranes.

other:

Article Title: Immunomodulatory impact of the A2A adenosine receptor on the profile of chemokines produced by neutrophils
Article Snippet: Leupeptin and aprotinin were obtained from ICN Biomedicals (Irvin, CA).

Article Title: Inhibition of Rho-associated kinase blocks agonist-induced Ca2+ sensitization of myosin phosphorylation and force in guinea-pig ileum
Article Snippet: Leupeptin, pepstatin A and DTT were from ICN Biomedicals, Inc. (Aurora, OH, USA).

Polyacrylamide Gel Electrophoresis:

Article Title: P-cadherin counteracts myosin II-B function: implications in melanoma progression
Article Snippet: .. Polyacrylamide gel electrophoresis (PAGE), Western blotting and immunoprecipitation experiments Cells were lysed using PBS containing 1% Triton X-100, 1% NP40 (Sigma, MO, US) and the following protease inhibitors: Leupeptin (10 μg/mL), aprotinin (10 μg/mL) (ICN Biomedicals, CA, US), phenylmethylsulfonyl fluoride (1.72 mM), NaF (100 μM), NaVO3 (500 μM), and Na4P2O7 (500 μg/mL) (Sigma, MO, US). .. After preparing lysates, protein concentration was measured using Rc-Dc protein assay kit (Bio-Rad Laboratories S.A.-N.V, Belgium).

Immunoprecipitation:

Article Title: P-cadherin counteracts myosin II-B function: implications in melanoma progression
Article Snippet: .. Polyacrylamide gel electrophoresis (PAGE), Western blotting and immunoprecipitation experiments Cells were lysed using PBS containing 1% Triton X-100, 1% NP40 (Sigma, MO, US) and the following protease inhibitors: Leupeptin (10 μg/mL), aprotinin (10 μg/mL) (ICN Biomedicals, CA, US), phenylmethylsulfonyl fluoride (1.72 mM), NaF (100 μM), NaVO3 (500 μM), and Na4P2O7 (500 μg/mL) (Sigma, MO, US). .. After preparing lysates, protein concentration was measured using Rc-Dc protein assay kit (Bio-Rad Laboratories S.A.-N.V, Belgium).

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    ICN Biomedicals leupeptin
    Leupeptin, supplied by ICN Biomedicals, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/leupeptin/product/ICN Biomedicals
    Average 92 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    leupeptin - by Bioz Stars, 2020-07
    92/100 stars
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