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Cell Signaling Technology Inc leupeptin
Leupeptin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 133 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/leupeptin/product/Cell Signaling Technology Inc
Average 94 stars, based on 133 article reviews
Price from $9.99 to $1999.99
leupeptin - by Bioz Stars, 2020-07
94/100 stars

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Radio Immunoprecipitation:

Article Title: Encapsulated Mulberry Fruit Extract Alleviates Changes in an Animal Model of Menopause with Metabolic Syndrome
Article Snippet: .. Western Blotting Analysis Adipose tissue was homogenized and lysed in RIPA (radioimmunoprecipitation assay) buffer (1 : 5, w /v ) (Cell Signaling Technology, USA) containing 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM Na2 EDTA, 1 mM EGTA, 1% NP-40, 1% sodium deoxycholate, 2.5 mM sodium pyrophosphate, 1 mM beta-glycerophosphate, 1 mM Na3 VO4 , 1 μ g/ml leupeptin, and 1 mM phenylmethanesulfonyl fluoride (PMSF) (Cell Signaling Technology, USA). .. The tissue homogenate supernatant of the middle layer of fat samples was isolated after the 12,000 × g centrifugation at 4°C for 10 minutes.

Protease Inhibitor:

Article Title: Human Head and Neck Squamous Cell Carcinoma–Associated Semaphorin 4D Induces Expansion of Myeloid-Derived Suppressor Cells
Article Snippet: .. Cells were harvested in cell lysis buffer (20mM Tris-HCl [pH 7.5], 150 mM NaCl, 1 mM Na2 EDTA, 1 mM EGTA, 1% Triton X-100, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, 1 mM Na3 VO4 , 1 μg/ml leupeptin) (catalog no. 9803) (Cell Signaling Technology, Danvers, MA) with the addition of 1× protease inhibitor cOmplete, Mini, EDTA-free (catalog no. 11836170001) (Roche Diagnostics, Indianapolis, IN). .. Whole-cell lysate was separated using SDS-PAGE.

Cell Culture:

Article Title: Protective effect of Korean Red Ginseng against FK506-induced damage in LLC-PK1 cells
Article Snippet: .. 2.4 Western blotting analysis LLC-PK1 cells cultured in wells of six-well plates were treated with 10 μg/mL KRG or 50 μg/mL KRG (for 24 h) and lysed with RIPA buffer [20mM Tris-HCl (pH 7.5), 150mM NaCl, 1mM Na2 EDTA, 1mM EGTA, 1% NP-40, 1% sodium deoxycholate, 2.5mM sodium pyrophosphate, 1mM beta-glycerophosphate, 1mM Na3 VO4 , 1 μg/mL leupeptin; Cell Signaling Technology, Beverly, MA, USA] supplemented with 1mM phenylmethylsulfonyl fluoride (PMSF) immediately before use. .. Protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Pittsburgh, PA, USA).

Lysis:

Article Title: Human Head and Neck Squamous Cell Carcinoma–Associated Semaphorin 4D Induces Expansion of Myeloid-Derived Suppressor Cells
Article Snippet: .. Cells were harvested in cell lysis buffer (20mM Tris-HCl [pH 7.5], 150 mM NaCl, 1 mM Na2 EDTA, 1 mM EGTA, 1% Triton X-100, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, 1 mM Na3 VO4 , 1 μg/ml leupeptin) (catalog no. 9803) (Cell Signaling Technology, Danvers, MA) with the addition of 1× protease inhibitor cOmplete, Mini, EDTA-free (catalog no. 11836170001) (Roche Diagnostics, Indianapolis, IN). .. Whole-cell lysate was separated using SDS-PAGE.

Article Title: ?-Arrestin 1 Inhibits the GTPase-Activating Protein Function of ARHGAP21, Promoting Activation of RhoA following Angiotensin II Type 1A Receptor Stimulation ▿
Article Snippet: .. Following stimulation, cells were lysed in 1× ice-cold Mg2+ lysis buffer (125 mM HEPES [pH 7.5], 750 mM NaCl, 5% Igepal CA-630, 50 mM MgCl2 , 5 mM EDTA, 10% glycerol, 10 μg/ml aprotonin, and 10 μg/ml leupeptin) (Upstate Cell Signaling, United Kingdom). .. Lysed cells were then incubated on ice for ∼10 min, after which they were scraped into prechilled 1.5-ml microcentrifuge tubes.

Article Title: Prostate cancer cell malignancy via modulation of HIF-1α pathway with isoflurane and propofol alone and in combination
Article Snippet: .. Western blotting Control or treated PC3 cells described above were washed with ice-cold PBS buffer and lysed on ice by physical disruption using a cell scraper in 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM Na2 EDTA, 2 mM EGTA, 1% Triton, 2.5 mM Na4 P2 O7 , 1 mM beta-glycerophosphate, 1 mM Na3 VO4 , 1 μ g ml−1 leupeptin, 1 mM phenyl methane sulphonyl fluoride and 1 mM dithiothreitol cell lysis buffer (Cell Signalling). ..

Article Title: Bioactive Cembranoids from the Soft Coral Genus Sinularia sp. in Borneo
Article Snippet: .. The cells were lysed in a lysis buffer (50 mM Tris–HCl (pH 7.5), 150 mM NaCl, 1% Nonidet P-40, 2 mM EDTA, 1 mM EGTA, 1 mM NaVO3 , 10 mM NaF, 1 mM dithiothreitol, 1 mM phenylmethylsulfonyl fluoride, 25 μg/mL aprotinin, and 25 μg/mL leupeptin) and kept on ice for 30 min. Antibodies against Bax, Bcl-2, caspase-9, and β-actin were purchased from Cell Signaling Technology (Bedford, MA, USA). .. Cell lysates were washed by centrifugation, and protein concentrations were determined using the BCATM protein assay kit (Thermo Fisher Scientific, Waltham, MA, USA) Aliquots of the lysates (30 μg of protein) were separated on a 12% SDS–polyacrylamide gel and transferred onto a polyvinylidene fluoride membrane (BIO-RAD) with a glycine transfer buffer (192 mM glycine, 25 mM Tris–HCl (pH 8.8), and 20% methanol ( v/v )).

Modification:

Article Title: Local acting Sticky-trap inhibits vascular endothelial growth factor dependent pathological angiogenesis in the eye
Article Snippet: .. Cells were then lysed in modified RIPA (mRIPA) buffer (50 mM Tris, pH 7.4, 150 mM NaCl, 1% NP-40, 0.25% sodium deoxycolate, 1 mM EDTA, 1 mM sodium orthovanadate, 10 mM β-glycerophosphate and protease inhibitors [1 mM phenylmethanesulfonyl fluoride (PMSF), 20 μg/ml leupeptin, and 20 μg/ml aprotinin]) and evaluated by immunoblot analysis using the following antibodies at a dilution of 1:1000: rabbit anti-VEGFR2 (55B11) (Cell Signaling, Danvers, MA, USA), rabbit anit-phospho-VEGFR2 Y1175 (Cell Signaling) and mouse anti-GAPDH (EMD Millipore, Billerica, MA, USA). .. Flow cytometry analysis For flow cytometry analysis, 1 × 106 cells were plated per well (9.6 cm2 /well) in a 6-well plate and cultured with or without doxycycline for 48 h, trypsinized and suspended into PBS containing 1% v/v of 7-AAD (BD Pharmingen, cat. # 559925) for detection of apoptotic cells.

Western Blot:

Article Title: Protective effect of Korean Red Ginseng against FK506-induced damage in LLC-PK1 cells
Article Snippet: .. 2.4 Western blotting analysis LLC-PK1 cells cultured in wells of six-well plates were treated with 10 μg/mL KRG or 50 μg/mL KRG (for 24 h) and lysed with RIPA buffer [20mM Tris-HCl (pH 7.5), 150mM NaCl, 1mM Na2 EDTA, 1mM EGTA, 1% NP-40, 1% sodium deoxycholate, 2.5mM sodium pyrophosphate, 1mM beta-glycerophosphate, 1mM Na3 VO4 , 1 μg/mL leupeptin; Cell Signaling Technology, Beverly, MA, USA] supplemented with 1mM phenylmethylsulfonyl fluoride (PMSF) immediately before use. .. Protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Pittsburgh, PA, USA).

Article Title: Encapsulated Mulberry Fruit Extract Alleviates Changes in an Animal Model of Menopause with Metabolic Syndrome
Article Snippet: .. Western Blotting Analysis Adipose tissue was homogenized and lysed in RIPA (radioimmunoprecipitation assay) buffer (1 : 5, w /v ) (Cell Signaling Technology, USA) containing 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM Na2 EDTA, 1 mM EGTA, 1% NP-40, 1% sodium deoxycholate, 2.5 mM sodium pyrophosphate, 1 mM beta-glycerophosphate, 1 mM Na3 VO4 , 1 μ g/ml leupeptin, and 1 mM phenylmethanesulfonyl fluoride (PMSF) (Cell Signaling Technology, USA). .. The tissue homogenate supernatant of the middle layer of fat samples was isolated after the 12,000 × g centrifugation at 4°C for 10 minutes.

Article Title: Prostate cancer cell malignancy via modulation of HIF-1α pathway with isoflurane and propofol alone and in combination
Article Snippet: .. Western blotting Control or treated PC3 cells described above were washed with ice-cold PBS buffer and lysed on ice by physical disruption using a cell scraper in 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM Na2 EDTA, 2 mM EGTA, 1% Triton, 2.5 mM Na4 P2 O7 , 1 mM beta-glycerophosphate, 1 mM Na3 VO4 , 1 μ g ml−1 leupeptin, 1 mM phenyl methane sulphonyl fluoride and 1 mM dithiothreitol cell lysis buffer (Cell Signalling). ..

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    Cell Signaling Technology Inc leupeptin
    Leupeptin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 133 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/leupeptin/product/Cell Signaling Technology Inc
    Average 94 stars, based on 133 article reviews
    Price from $9.99 to $1999.99
    leupeptin - by Bioz Stars, 2020-07
    94/100 stars
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