Structured Review

Bio Basic Canada leupeptin
Leupeptin, supplied by Bio Basic Canada, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/leupeptin/product/Bio Basic Canada
Average 90 stars, based on 1 article reviews
Price from $9.99 to $1999.99
leupeptin - by Bioz Stars, 2020-07
90/100 stars

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Modification:

Article Title: The ErbB2ΔEx16 splice variant is a major oncogenic driver in breast cancer that promotes a pro-metastatic tumor microenvironment
Article Snippet: .. Immunoblot analysis Flash frozen tumor tissue was disrupted by mortar and pestle in liquid nitrogen, and lysed in a modified Hepes lysis buffer (50mM Hepes pH 7.5 (Bioshop HEP001), 150mM NaCl (Biobasic SB0476), 10% Glycerol (GB0232), 0.5% NP-40 (Biobasic NDB0385), 1mM EDTA pH 8.0 (Biobasic EB0185), 10mM NaF (Bioshop SFL001), 1mM PMSF (Bioshop PMS123), 10mM β Glycerophosphate (Bioshop GYP001), 1mM Na3 VO4 (Bioshop SOV664), 10μg/ml Aprotinin (Biobasic AD0153), 10μg/ml Leupeptin (Biobasic LDJ691). .. Lysates were quantified by Bradford assay, and reduced in SDS loading buffer with β-mercaptoethanol (Sigma M6250).

Lysis:

Article Title: The ErbB2ΔEx16 splice variant is a major oncogenic driver in breast cancer that promotes a pro-metastatic tumor microenvironment
Article Snippet: .. Immunoblot analysis Flash frozen tumor tissue was disrupted by mortar and pestle in liquid nitrogen, and lysed in a modified Hepes lysis buffer (50mM Hepes pH 7.5 (Bioshop HEP001), 150mM NaCl (Biobasic SB0476), 10% Glycerol (GB0232), 0.5% NP-40 (Biobasic NDB0385), 1mM EDTA pH 8.0 (Biobasic EB0185), 10mM NaF (Bioshop SFL001), 1mM PMSF (Bioshop PMS123), 10mM β Glycerophosphate (Bioshop GYP001), 1mM Na3 VO4 (Bioshop SOV664), 10μg/ml Aprotinin (Biobasic AD0153), 10μg/ml Leupeptin (Biobasic LDJ691). .. Lysates were quantified by Bradford assay, and reduced in SDS loading buffer with β-mercaptoethanol (Sigma M6250).

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    Bio Basic Canada leupeptin
    Leupeptin, supplied by Bio Basic Canada, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/leupeptin/product/Bio Basic Canada
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    leupeptin - by Bioz Stars, 2020-07
    90/100 stars
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