Structured Review

Applichem leupeptin
Inhibitory analysis of L1 proteolysis. HeLa (A) or HaCaT (B) cells were infected with HPV16 PsV in the presence or absence of the following inhibitors: NH 4 Cl (20 mM), bafilomycin A1 (10 nM), E-64 (100 μM), <t>leupeptin</t> (100 μM), CA-074 or
Leupeptin, supplied by Applichem, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/leupeptin/product/Applichem
Average 93 stars, based on 10 article reviews
Price from $9.99 to $1999.99
leupeptin - by Bioz Stars, 2020-07
93/100 stars

Images

1) Product Images from "Kallikrein-8 Proteolytically Processes Human Papillomaviruses in the Extracellular Space To Facilitate Entry into Host Cells"

Article Title: Kallikrein-8 Proteolytically Processes Human Papillomaviruses in the Extracellular Space To Facilitate Entry into Host Cells

Journal: Journal of Virology

doi: 10.1128/JVI.00234-15

Inhibitory analysis of L1 proteolysis. HeLa (A) or HaCaT (B) cells were infected with HPV16 PsV in the presence or absence of the following inhibitors: NH 4 Cl (20 mM), bafilomycin A1 (10 nM), E-64 (100 μM), leupeptin (100 μM), CA-074 or
Figure Legend Snippet: Inhibitory analysis of L1 proteolysis. HeLa (A) or HaCaT (B) cells were infected with HPV16 PsV in the presence or absence of the following inhibitors: NH 4 Cl (20 mM), bafilomycin A1 (10 nM), E-64 (100 μM), leupeptin (100 μM), CA-074 or

Techniques Used: Infection

Related Articles

Lysis:

Article Title: Heparan Sulfate Induces Necroptosis in Murine Cardiomyocytes: A Medical-In silico Approach Combining In vitro Experiments and Machine Learning
Article Snippet: .. Cardiomyocytes were washed with PBS and lysed using Triton lysis buffer [300 mM NaCl (Roth, Karlsruhe, Germany), 20 mM TRIS (pH 7.4) (Merck, MA, USA), 1% Triton-X100 (Sigma-Aldrich), 200 mM PMSF (Roth), 1 mM DTT (Gerbu, Heidelberg, Germany), 2 mg/ml leupeptin (AppliChem), and 1 mg/ml pepstatin (AppliChem)] ( , ). .. The supernatant was transferred to a new tube, and the protein concentrations were determined by the Bradford method (Roti-Quant, Roth).

Article Title: Heparan Sulfate Induces Necroptosis in Murine Cardiomyocytes: A Medical-In silico Approach Combining In vitro Experiments and Machine Learning
Article Snippet: .. Western Blot Analysis Cardiomyocytes were washed with PBS and lysed using Triton lysis buffer [300 mM NaCl (Roth, Karlsruhe, Germany), 20 mM TRIS (pH 7.4) (Merck, MA, USA), 1% Triton-X100 (Sigma-Aldrich), 200 mM PMSF (Roth), 1 mM DTT (Gerbu, Heidelberg, Germany), 2 mg/ml leupeptin (AppliChem), and 1 mg/ml pepstatin (AppliChem)] ( , ). .. The supernatant was transferred to a new tube, and the protein concentrations were determined by the Bradford method (Roti-Quant, Roth).

Protein Extraction:

Article Title: Intracellular Staphylococcus aureus eludes selective autophagy by activating a host cell kinase
Article Snippet: .. For protein extraction cells were lysed in TPNE buffer (1% Triton X-100, 2 mM EDTA [Carl Roth, 8043], 1 mM PMSF [Sigma, P7626] and 1 µg/mL of leupeptin [Applichem, A2183], aprotinin [Applichem, A2132], chymostatin [Applichem, A2144] and pepstatin [Applichem, A2205] each, in PBS adjusted to 300 mM NaCl [Carl Roth, 3957.1]). .. Protein concentration was measured via Pierce BCA protein assay kit (Thermo Fisher Scientific, 23225).

Protease Inhibitor:

Article Title: Basic domain of telomere guardian TRF2 reduces D-loop unwinding whereas Rap1 restores it
Article Snippet: .. The pellet was dissolved in buffer containing 50 mM sodium phosphate, pH 8.0, 500 mM NaCl, 10 mM imidazole, 0.5% Tween-20, 10% glycerol, 5 μM Leupeptin, 4 μM Pepstatin A (Applichem) along with protease inhibitor cocktail cOmplete tablets EDTA-free (Roche). .. The cell suspension was sonicated for 3 min of process time with 1 s pulse and 2 s of cooling on ice (Misonix).

Western Blot:

Article Title: Heparan Sulfate Induces Necroptosis in Murine Cardiomyocytes: A Medical-In silico Approach Combining In vitro Experiments and Machine Learning
Article Snippet: .. Western Blot Analysis Cardiomyocytes were washed with PBS and lysed using Triton lysis buffer [300 mM NaCl (Roth, Karlsruhe, Germany), 20 mM TRIS (pH 7.4) (Merck, MA, USA), 1% Triton-X100 (Sigma-Aldrich), 200 mM PMSF (Roth), 1 mM DTT (Gerbu, Heidelberg, Germany), 2 mg/ml leupeptin (AppliChem), and 1 mg/ml pepstatin (AppliChem)] ( , ). .. The supernatant was transferred to a new tube, and the protein concentrations were determined by the Bradford method (Roti-Quant, Roth).

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  • 93
    Applichem leupeptin
    Inhibitory analysis of L1 proteolysis. HeLa (A) or HaCaT (B) cells were infected with HPV16 PsV in the presence or absence of the following inhibitors: NH 4 Cl (20 mM), bafilomycin A1 (10 nM), E-64 (100 μM), <t>leupeptin</t> (100 μM), CA-074 or
    Leupeptin, supplied by Applichem, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/leupeptin/product/Applichem
    Average 93 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    leupeptin - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    90
    Applichem leupeptine
    Inhibitory analysis of L1 proteolysis. HeLa (A) or HaCaT (B) cells were infected with HPV16 PsV in the presence or absence of the following inhibitors: NH 4 Cl (20 mM), bafilomycin A1 (10 nM), E-64 (100 μM), <t>leupeptin</t> (100 μM), CA-074 or
    Leupeptine, supplied by Applichem, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/leupeptine/product/Applichem
    Average 90 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    leupeptine - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    Image Search Results


    Inhibitory analysis of L1 proteolysis. HeLa (A) or HaCaT (B) cells were infected with HPV16 PsV in the presence or absence of the following inhibitors: NH 4 Cl (20 mM), bafilomycin A1 (10 nM), E-64 (100 μM), leupeptin (100 μM), CA-074 or

    Journal: Journal of Virology

    Article Title: Kallikrein-8 Proteolytically Processes Human Papillomaviruses in the Extracellular Space To Facilitate Entry into Host Cells

    doi: 10.1128/JVI.00234-15

    Figure Lengend Snippet: Inhibitory analysis of L1 proteolysis. HeLa (A) or HaCaT (B) cells were infected with HPV16 PsV in the presence or absence of the following inhibitors: NH 4 Cl (20 mM), bafilomycin A1 (10 nM), E-64 (100 μM), leupeptin (100 μM), CA-074 or

    Article Snippet: AEBSF [4-(2-aminoethyl)benzenesulfonyl fluoride], leupeptin, ZnCl2 , and ZnSO4 were from Applichem.

    Techniques: Infection