Review



leptospira interrogans li serovar icterohaemorrhagiae strain rga  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    ATCC leptospira interrogans li serovar icterohaemorrhagiae strain rga
    Purification and characterization of <t>Leptospira</t> <t>interrogans</t> TIM (LiTIM). ( A ) 12% SDS-PAGE showing the final purified protein used for further characterisation. ( B ) Far-UV CD spectrum and ( C ) tryptophan intrinsic fluorescence spectrum of the purified protein confirm a well folded form in solution. ( D ) Crystals obtained for the apo form of LiTIM. The crystal in the lower panel was soaked with substrate to obtain the holo form of the protein. ( E ) Analytical gel filtration chramatography indicates that it is a dimer in the solution (PfTIM WT, known to be dimeric, was used as the control ). ( F ) LiTIM protein sequence (251 residues)-catalytic residues in red bold, residues that showed conformational change upon substrate binding in bold underline, and other periferal, conserved residues that interact with substrate are shown in underlined letters. ( G ) LC-ESI/MS of the purified LiTIM protein agrees with the expected mass.
    Leptospira Interrogans Li Serovar Icterohaemorrhagiae Strain Rga, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/leptospira interrogans li serovar icterohaemorrhagiae strain rga/product/ATCC
    Average 99 stars, based on 26 article reviews
    leptospira interrogans li serovar icterohaemorrhagiae strain rga - by Bioz Stars, 2026-02
    99/100 stars

    Images

    1) Product Images from "Leptospira interrogans triosephosphate isomerase: exploring the structural determinants of stability, high reaction rate and specificity"

    Article Title: Leptospira interrogans triosephosphate isomerase: exploring the structural determinants of stability, high reaction rate and specificity

    Journal: bioRxiv

    doi: 10.1101/2020.12.04.412312

    Purification and characterization of Leptospira interrogans TIM (LiTIM). ( A ) 12% SDS-PAGE showing the final purified protein used for further characterisation. ( B ) Far-UV CD spectrum and ( C ) tryptophan intrinsic fluorescence spectrum of the purified protein confirm a well folded form in solution. ( D ) Crystals obtained for the apo form of LiTIM. The crystal in the lower panel was soaked with substrate to obtain the holo form of the protein. ( E ) Analytical gel filtration chramatography indicates that it is a dimer in the solution (PfTIM WT, known to be dimeric, was used as the control ). ( F ) LiTIM protein sequence (251 residues)-catalytic residues in red bold, residues that showed conformational change upon substrate binding in bold underline, and other periferal, conserved residues that interact with substrate are shown in underlined letters. ( G ) LC-ESI/MS of the purified LiTIM protein agrees with the expected mass.
    Figure Legend Snippet: Purification and characterization of Leptospira interrogans TIM (LiTIM). ( A ) 12% SDS-PAGE showing the final purified protein used for further characterisation. ( B ) Far-UV CD spectrum and ( C ) tryptophan intrinsic fluorescence spectrum of the purified protein confirm a well folded form in solution. ( D ) Crystals obtained for the apo form of LiTIM. The crystal in the lower panel was soaked with substrate to obtain the holo form of the protein. ( E ) Analytical gel filtration chramatography indicates that it is a dimer in the solution (PfTIM WT, known to be dimeric, was used as the control ). ( F ) LiTIM protein sequence (251 residues)-catalytic residues in red bold, residues that showed conformational change upon substrate binding in bold underline, and other periferal, conserved residues that interact with substrate are shown in underlined letters. ( G ) LC-ESI/MS of the purified LiTIM protein agrees with the expected mass.

    Techniques Used: Purification, SDS Page, Fluorescence, Filtration, Control, Sequencing, Binding Assay


    Figure Legend Snippet:

    Techniques Used:



    Similar Products

    leptospira interrogans li serovar icterohaemorrhagiae strain rga  (ATCC)
    99
    ATCC leptospira interrogans li serovar icterohaemorrhagiae strain rga
    Purification and characterization of <t>Leptospira</t> <t>interrogans</t> TIM (LiTIM). ( A ) 12% SDS-PAGE showing the final purified protein used for further characterisation. ( B ) Far-UV CD spectrum and ( C ) tryptophan intrinsic fluorescence spectrum of the purified protein confirm a well folded form in solution. ( D ) Crystals obtained for the apo form of LiTIM. The crystal in the lower panel was soaked with substrate to obtain the holo form of the protein. ( E ) Analytical gel filtration chramatography indicates that it is a dimer in the solution (PfTIM WT, known to be dimeric, was used as the control ). ( F ) LiTIM protein sequence (251 residues)-catalytic residues in red bold, residues that showed conformational change upon substrate binding in bold underline, and other periferal, conserved residues that interact with substrate are shown in underlined letters. ( G ) LC-ESI/MS of the purified LiTIM protein agrees with the expected mass.
    Leptospira Interrogans Li Serovar Icterohaemorrhagiae Strain Rga, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/leptospira interrogans li serovar icterohaemorrhagiae strain rga/product/ATCC
    Average 99 stars, based on 1 article reviews
    leptospira interrogans li serovar icterohaemorrhagiae strain rga - by Bioz Stars, 2026-02
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Purification and characterization of Leptospira interrogans TIM (LiTIM). ( A ) 12% SDS-PAGE showing the final purified protein used for further characterisation. ( B ) Far-UV CD spectrum and ( C ) tryptophan intrinsic fluorescence spectrum of the purified protein confirm a well folded form in solution. ( D ) Crystals obtained for the apo form of LiTIM. The crystal in the lower panel was soaked with substrate to obtain the holo form of the protein. ( E ) Analytical gel filtration chramatography indicates that it is a dimer in the solution (PfTIM WT, known to be dimeric, was used as the control ). ( F ) LiTIM protein sequence (251 residues)-catalytic residues in red bold, residues that showed conformational change upon substrate binding in bold underline, and other periferal, conserved residues that interact with substrate are shown in underlined letters. ( G ) LC-ESI/MS of the purified LiTIM protein agrees with the expected mass.

    Journal: bioRxiv

    Article Title: Leptospira interrogans triosephosphate isomerase: exploring the structural determinants of stability, high reaction rate and specificity

    doi: 10.1101/2020.12.04.412312

    Figure Lengend Snippet: Purification and characterization of Leptospira interrogans TIM (LiTIM). ( A ) 12% SDS-PAGE showing the final purified protein used for further characterisation. ( B ) Far-UV CD spectrum and ( C ) tryptophan intrinsic fluorescence spectrum of the purified protein confirm a well folded form in solution. ( D ) Crystals obtained for the apo form of LiTIM. The crystal in the lower panel was soaked with substrate to obtain the holo form of the protein. ( E ) Analytical gel filtration chramatography indicates that it is a dimer in the solution (PfTIM WT, known to be dimeric, was used as the control ). ( F ) LiTIM protein sequence (251 residues)-catalytic residues in red bold, residues that showed conformational change upon substrate binding in bold underline, and other periferal, conserved residues that interact with substrate are shown in underlined letters. ( G ) LC-ESI/MS of the purified LiTIM protein agrees with the expected mass.

    Article Snippet: Triosephosphate isomerase gene ( tim gene ID: LEP2GSC113_RS0110880) was amplified from five days old Ellinghausen-McCullough-Johnson-Harris (EMJH) culture of Leptospira interrogans (Li) serovar Icterohaemorrhagiae strain RGA (Regional Medical Research Centre, Indian Council for Medical Research, Port Blair, India; ATCC: 23581.

    Techniques: Purification, SDS Page, Fluorescence, Filtration, Control, Sequencing, Binding Assay

    Journal: bioRxiv

    Article Title: Leptospira interrogans triosephosphate isomerase: exploring the structural determinants of stability, high reaction rate and specificity

    doi: 10.1101/2020.12.04.412312

    Figure Lengend Snippet:

    Article Snippet: Triosephosphate isomerase gene ( tim gene ID: LEP2GSC113_RS0110880) was amplified from five days old Ellinghausen-McCullough-Johnson-Harris (EMJH) culture of Leptospira interrogans (Li) serovar Icterohaemorrhagiae strain RGA (Regional Medical Research Centre, Indian Council for Medical Research, Port Blair, India; ATCC: 23581.

    Techniques: