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Waters Corporation lc msms analysis
Lc Msms Analysis, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lc msms analysis/product/Waters Corporation
Average 88 stars, based on 1 article reviews
Price from $9.99 to $1999.99
lc msms analysis - by Bioz Stars, 2020-07
88/100 stars

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Sample Prep:

Article Title: Presence of Emerging Per- and Polyfluoroalkyl Substances (PFASs) in River and Drinking Water near a Fluorochemical Production Plant in the Netherlands
Article Snippet: .. The water sample preparation and LC-MSMS analysis is based on previous studies., Briefly, a volume of 250 mL water was spiked with internal standards and loaded onto a WAX SPE cartridge (Waters; 3 mL, 60 mg) preconditioned with 4 mL methanol and 4 mL water. ..

Mass Spectrometry:

Article Title: Next-Generation Sequencing and Quantitative Proteomics of Hutchinson-Gilford progeria syndrome-derived cells point to a role of nucleotide metabolism in premature aging
Article Snippet: .. LC-MSMS analysis The 10 dried-down fractions were analyzed in a nano-Acquity liquid chromatograph (Waters) coupled to a LTQ-Orbitrap Velos (Thermo Scientific) mass spectrometer. .. Tryptic labeled peptides were resuspended in 2% ACN/1% formic acid (FA) solution and an aliquot (corresponding to 500 ng) was injected for their chromatographic separation.

Article Title: Prediction of Drug Permeability Using In Vitro Blood–Brain Barrier Models with Human Induced Pluripotent Stem Cell-Derived Brain Microvascular Endothelial Cells
Article Snippet: .. Drugs at a concentration of 10 μM ( and ) diluted in HBSS were added to apical wells for A-to-B samples or basolateral wells for B-to-A samples; the cells were then incubated at 37°C with agitation and 50-μL samples from the basolateral wells (A-to-B samples) and apical wells (B-to-A samples) were collected at 60 and 80 min. Drug concentrations were measured by LC/MS analysis (LC: Prominence, MS: LCMS-2010EV; Shimazu Co., Japan), and LC/MSMS analysis (LC: Acquity; Waters Co., MA; MS: TSQ QUANTIVA; Thermo Fisher Scientific, Inc.) was used for digoxin measurement. .. For the data analyses, Papp (apparent permeability coefficients; cm/sec), and efflux ratios were calculated using the following formulas: \documentclass{aastex}\usepackage{amsbsy}\usepackage{amsfonts}\usepackage{amssymb}\usepackage{bm}\usepackage{mathrsfs}\usepackage{pifont}\usepackage{stmaryrd}\usepackage{textcomp}\usepackage{portland, xspace}\usepackage{amsmath, amsxtra}\usepackage{upgreek}\pagestyle{empty}\DeclareMathSizes{10}{9}{7}{6}\begin{document} \begin{align*} {\rm Papp}{ \rm{ }} \left(\hbox{cm / sec} \right) \; = \;\hbox{dQ / dT} \; \times \;{\rm A} \; \times \;{\rm C}0 \end{align*} \end{document} \documentclass{aastex}\usepackage{amsbsy}\usepackage{amsfonts}\usepackage{amssymb}\usepackage{bm}\usepackage{mathrsfs}\usepackage{pifont}\usepackage{stmaryrd}\usepackage{textcomp}\usepackage{portland, xspace}\usepackage{amsmath, amsxtra}\usepackage{upgreek}\pagestyle{empty}\DeclareMathSizes{10}{9}{7}{6}\begin{document} \begin{align*} {\rm Efflux}{ \rm{ }} \ {\rm ratio} \; = \;{\rm Papp}{ \rm{ }} \left( {{\rm B} - {\rm to} - {\rm A}} \right) / {\rm Papp}{ \rm{ }} \left( {{\rm A} - {\rm to} - {\rm B}} \right) \end{align*} \end{document} where dQ/dT is the amount of drug transported per unit time; A is the membrane surface area; and C0 is the donor concentration at time 0.

Concentration Assay:

Article Title: Prediction of Drug Permeability Using In Vitro Blood–Brain Barrier Models with Human Induced Pluripotent Stem Cell-Derived Brain Microvascular Endothelial Cells
Article Snippet: .. Drugs at a concentration of 10 μM ( and ) diluted in HBSS were added to apical wells for A-to-B samples or basolateral wells for B-to-A samples; the cells were then incubated at 37°C with agitation and 50-μL samples from the basolateral wells (A-to-B samples) and apical wells (B-to-A samples) were collected at 60 and 80 min. Drug concentrations were measured by LC/MS analysis (LC: Prominence, MS: LCMS-2010EV; Shimazu Co., Japan), and LC/MSMS analysis (LC: Acquity; Waters Co., MA; MS: TSQ QUANTIVA; Thermo Fisher Scientific, Inc.) was used for digoxin measurement. .. For the data analyses, Papp (apparent permeability coefficients; cm/sec), and efflux ratios were calculated using the following formulas: \documentclass{aastex}\usepackage{amsbsy}\usepackage{amsfonts}\usepackage{amssymb}\usepackage{bm}\usepackage{mathrsfs}\usepackage{pifont}\usepackage{stmaryrd}\usepackage{textcomp}\usepackage{portland, xspace}\usepackage{amsmath, amsxtra}\usepackage{upgreek}\pagestyle{empty}\DeclareMathSizes{10}{9}{7}{6}\begin{document} \begin{align*} {\rm Papp}{ \rm{ }} \left(\hbox{cm / sec} \right) \; = \;\hbox{dQ / dT} \; \times \;{\rm A} \; \times \;{\rm C}0 \end{align*} \end{document} \documentclass{aastex}\usepackage{amsbsy}\usepackage{amsfonts}\usepackage{amssymb}\usepackage{bm}\usepackage{mathrsfs}\usepackage{pifont}\usepackage{stmaryrd}\usepackage{textcomp}\usepackage{portland, xspace}\usepackage{amsmath, amsxtra}\usepackage{upgreek}\pagestyle{empty}\DeclareMathSizes{10}{9}{7}{6}\begin{document} \begin{align*} {\rm Efflux}{ \rm{ }} \ {\rm ratio} \; = \;{\rm Papp}{ \rm{ }} \left( {{\rm B} - {\rm to} - {\rm A}} \right) / {\rm Papp}{ \rm{ }} \left( {{\rm A} - {\rm to} - {\rm B}} \right) \end{align*} \end{document} where dQ/dT is the amount of drug transported per unit time; A is the membrane surface area; and C0 is the donor concentration at time 0.

Incubation:

Article Title: Prediction of Drug Permeability Using In Vitro Blood–Brain Barrier Models with Human Induced Pluripotent Stem Cell-Derived Brain Microvascular Endothelial Cells
Article Snippet: .. Drugs at a concentration of 10 μM ( and ) diluted in HBSS were added to apical wells for A-to-B samples or basolateral wells for B-to-A samples; the cells were then incubated at 37°C with agitation and 50-μL samples from the basolateral wells (A-to-B samples) and apical wells (B-to-A samples) were collected at 60 and 80 min. Drug concentrations were measured by LC/MS analysis (LC: Prominence, MS: LCMS-2010EV; Shimazu Co., Japan), and LC/MSMS analysis (LC: Acquity; Waters Co., MA; MS: TSQ QUANTIVA; Thermo Fisher Scientific, Inc.) was used for digoxin measurement. .. For the data analyses, Papp (apparent permeability coefficients; cm/sec), and efflux ratios were calculated using the following formulas: \documentclass{aastex}\usepackage{amsbsy}\usepackage{amsfonts}\usepackage{amssymb}\usepackage{bm}\usepackage{mathrsfs}\usepackage{pifont}\usepackage{stmaryrd}\usepackage{textcomp}\usepackage{portland, xspace}\usepackage{amsmath, amsxtra}\usepackage{upgreek}\pagestyle{empty}\DeclareMathSizes{10}{9}{7}{6}\begin{document} \begin{align*} {\rm Papp}{ \rm{ }} \left(\hbox{cm / sec} \right) \; = \;\hbox{dQ / dT} \; \times \;{\rm A} \; \times \;{\rm C}0 \end{align*} \end{document} \documentclass{aastex}\usepackage{amsbsy}\usepackage{amsfonts}\usepackage{amssymb}\usepackage{bm}\usepackage{mathrsfs}\usepackage{pifont}\usepackage{stmaryrd}\usepackage{textcomp}\usepackage{portland, xspace}\usepackage{amsmath, amsxtra}\usepackage{upgreek}\pagestyle{empty}\DeclareMathSizes{10}{9}{7}{6}\begin{document} \begin{align*} {\rm Efflux}{ \rm{ }} \ {\rm ratio} \; = \;{\rm Papp}{ \rm{ }} \left( {{\rm B} - {\rm to} - {\rm A}} \right) / {\rm Papp}{ \rm{ }} \left( {{\rm A} - {\rm to} - {\rm B}} \right) \end{align*} \end{document} where dQ/dT is the amount of drug transported per unit time; A is the membrane surface area; and C0 is the donor concentration at time 0.

Liquid Chromatography with Mass Spectroscopy:

Article Title: Prediction of Drug Permeability Using In Vitro Blood–Brain Barrier Models with Human Induced Pluripotent Stem Cell-Derived Brain Microvascular Endothelial Cells
Article Snippet: .. Drugs at a concentration of 10 μM ( and ) diluted in HBSS were added to apical wells for A-to-B samples or basolateral wells for B-to-A samples; the cells were then incubated at 37°C with agitation and 50-μL samples from the basolateral wells (A-to-B samples) and apical wells (B-to-A samples) were collected at 60 and 80 min. Drug concentrations were measured by LC/MS analysis (LC: Prominence, MS: LCMS-2010EV; Shimazu Co., Japan), and LC/MSMS analysis (LC: Acquity; Waters Co., MA; MS: TSQ QUANTIVA; Thermo Fisher Scientific, Inc.) was used for digoxin measurement. .. For the data analyses, Papp (apparent permeability coefficients; cm/sec), and efflux ratios were calculated using the following formulas: \documentclass{aastex}\usepackage{amsbsy}\usepackage{amsfonts}\usepackage{amssymb}\usepackage{bm}\usepackage{mathrsfs}\usepackage{pifont}\usepackage{stmaryrd}\usepackage{textcomp}\usepackage{portland, xspace}\usepackage{amsmath, amsxtra}\usepackage{upgreek}\pagestyle{empty}\DeclareMathSizes{10}{9}{7}{6}\begin{document} \begin{align*} {\rm Papp}{ \rm{ }} \left(\hbox{cm / sec} \right) \; = \;\hbox{dQ / dT} \; \times \;{\rm A} \; \times \;{\rm C}0 \end{align*} \end{document} \documentclass{aastex}\usepackage{amsbsy}\usepackage{amsfonts}\usepackage{amssymb}\usepackage{bm}\usepackage{mathrsfs}\usepackage{pifont}\usepackage{stmaryrd}\usepackage{textcomp}\usepackage{portland, xspace}\usepackage{amsmath, amsxtra}\usepackage{upgreek}\pagestyle{empty}\DeclareMathSizes{10}{9}{7}{6}\begin{document} \begin{align*} {\rm Efflux}{ \rm{ }} \ {\rm ratio} \; = \;{\rm Papp}{ \rm{ }} \left( {{\rm B} - {\rm to} - {\rm A}} \right) / {\rm Papp}{ \rm{ }} \left( {{\rm A} - {\rm to} - {\rm B}} \right) \end{align*} \end{document} where dQ/dT is the amount of drug transported per unit time; A is the membrane surface area; and C0 is the donor concentration at time 0.

Recombinant:

Article Title: Vv-AMP1, a ripening induced peptide from Vitis vinifera shows strong antifungal activity
Article Snippet: .. Forty five μg recombinant Vv-AMP1 peptide was digested with the ProteoExtracttm all-in-one trypsin digest kit (Calbiochem, La Jolla, CA, USA) and subjected to LC/MSMS analysis on a Waters API Q-TOF Ultima instrument. ..

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    Waters Corporation lc msms analysis
    Lc Msms Analysis, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lc msms analysis/product/Waters Corporation
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lc msms analysis - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    92
    Waters Corporation acquity h class ultra performance lc system
    Acquity H Class Ultra Performance Lc System, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acquity h class ultra performance lc system/product/Waters Corporation
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    acquity h class ultra performance lc system - by Bioz Stars, 2020-07
    92/100 stars
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