Structured Review

Leica Microsystems las af 6 2 software
Las Af 6 2 Software, supplied by Leica Microsystems, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/las af 6 2 software/product/Leica Microsystems
Average 92 stars, based on 5 article reviews
Price from $9.99 to $1999.99
las af 6 2 software - by Bioz Stars, 2020-04
92/100 stars

Images

Related Articles

Diagnostic Assay:

Article Title: Multiple myeloma–derived MMP-13 mediates osteoclast fusogenesis and osteolytic disease
Article Snippet: .. The number of OCLs per well, the number of nuclei per OCL, and OCL size were quantified using an Olympus IX70 microscope equipped with a 20/0.40 numeric aperture objective lens and SPOT software (Diagnostic Instruments) or a Leica DMI 6000B fluorescence microscope equipped with an HCX PL S-APO 10×/NA 0.3 objective lens, coupled with a Leica DFC365 FX digital camera and operated by LAS AF 6.2 software (Leica Microsystems). .. Images of resorption lacunae were captured using an OMAX 40X-2000X Digital Microscope with ScopeImage 9.0 software (BioImager) or Magnafire 4.1 software (Optronics).

Fluorescence:

Article Title: Highly efficient generation of airway and lung epithelial cells from human pluripotent stem cells
Article Snippet: .. Samples were visualized and imaged using motorized Leica DMI 6000B fluorescence microscope coupled with Leica DFC365 FX digital camera and operated by LAS AF 6.2 software (Leica Microsystems GmbH, Wetzlar, Germany). ..

Article Title: Conditional PDK1 ablation promotes epidermal and T cell-mediated dysfunctions leading to inflammatory skin disease
Article Snippet: .. Images were obtained using LAS AF 6.2 software on a motorized Leica DMI 6000B fluorescence microscope (Leica Microsystems GmbH, Wetzlar, Germany). .. T cell transfer and bone marrow reconstitution T cells were purified from spleen and peripheral lymph nodes of PDK1-CKO and -CHET mice by anti CD3ε microbeads (Miltenyi Biotec).

Article Title: Short-term high-fat feeding induces islet macrophage infiltration and β-cell replication independently of insulin resistance in mice
Article Snippet: .. Images were recorded using LAS AF 6.2 software on a motorized Leica DMI 600B fluorescence microscope (Leica Microsystems, Wetzlar, Germany). ..

Article Title: The in vitro generation of lung and airway progenitor cells from human pluripotent stem cells
Article Snippet: .. Mini Microcentrifuge Motorized Leica DMI 6000B fluorescence microscope coupled with Leica DFC365 FX and DFC450 digital cameras and operated by LAS AF 6.2 software (Leica Microsystems GmbH, Wetzlar, Germany) Tabletop centrifuge (Eppendorf, Centrifuge 5810 R) Water bath set at 37°C (Fisher Scientific, IsoTemp 210) 1.7 mL Posi-Click Microcentrifuge tubes (Denville Scientific, cat. no. C2170) 6-well Ultra-Low Attachment plate (Corning, cat. no. 3471) 6-well tissue culture-treated plate (Falcon, cat. no. 353046) 24-well tissue culture-treated plate (Falcon, cat. no. 353047) 48-well tissue culture-treated plate (Falcon, cat. no. 353078) 10 cm2 tissue culture-treated plates (Falcon, cat. no. 353003) 15 mL polypropylene conical centrifuge tube (Santa Cruz, cat. no. sc-200249) 50 mL polypropylene conical centrifuge tube (Santa Cruz, cat. no. sc-200252) Cell passaging tool (Life Technologies, cat. no. 23181-010) Cell Scraper (Sarstedt, cat. no. 83.1830) CryoTube vials (Nunc, cat. no. 375418) P1000 Barrier Pipette Tips (Denville Scientific, cat. no. P1126) P200 Barrier Pipette Tips (Denville Scientific, cat. no. P1122) P20 Barrier Pipette Tips (Denville Scientific, cat. no. 1121) P10 Barrier Pipette Tips (Denville Scientific, cat. no. P1096-FR) Serological Pipette – 2 mL, individually wrapped (Santa Cruz, cat. no. sc-200276) Serological Pipette – 5 mL, individually wrapped (Santa Cruz, cat. no. sc-200279) Serological Pipette – 10 mL, individually wrapped (Santa Cruz, cat. no. sc-200281) Serological Pipette – 25 mL, individually wrapped (Santa Cruz, cat. no. sc-200283) Serological Pipette – 50 mL, individually wrapped (Santa Cruz, cat. no. sc-213234) Sterile Filter Systems, disposable (0.22 uM, PES) (Corning, cat. no. 431097) Steriflip-GP Filter, 50ml, 0.22μm (EMD Millipore, cat. no. EW-29969-24) ..

Article Title: Multiple myeloma–derived MMP-13 mediates osteoclast fusogenesis and osteolytic disease
Article Snippet: .. The number of OCLs per well, the number of nuclei per OCL, and OCL size were quantified using an Olympus IX70 microscope equipped with a 20/0.40 numeric aperture objective lens and SPOT software (Diagnostic Instruments) or a Leica DMI 6000B fluorescence microscope equipped with an HCX PL S-APO 10×/NA 0.3 objective lens, coupled with a Leica DFC365 FX digital camera and operated by LAS AF 6.2 software (Leica Microsystems). .. Images of resorption lacunae were captured using an OMAX 40X-2000X Digital Microscope with ScopeImage 9.0 software (BioImager) or Magnafire 4.1 software (Optronics).

Passaging:

Article Title: The in vitro generation of lung and airway progenitor cells from human pluripotent stem cells
Article Snippet: .. Mini Microcentrifuge Motorized Leica DMI 6000B fluorescence microscope coupled with Leica DFC365 FX and DFC450 digital cameras and operated by LAS AF 6.2 software (Leica Microsystems GmbH, Wetzlar, Germany) Tabletop centrifuge (Eppendorf, Centrifuge 5810 R) Water bath set at 37°C (Fisher Scientific, IsoTemp 210) 1.7 mL Posi-Click Microcentrifuge tubes (Denville Scientific, cat. no. C2170) 6-well Ultra-Low Attachment plate (Corning, cat. no. 3471) 6-well tissue culture-treated plate (Falcon, cat. no. 353046) 24-well tissue culture-treated plate (Falcon, cat. no. 353047) 48-well tissue culture-treated plate (Falcon, cat. no. 353078) 10 cm2 tissue culture-treated plates (Falcon, cat. no. 353003) 15 mL polypropylene conical centrifuge tube (Santa Cruz, cat. no. sc-200249) 50 mL polypropylene conical centrifuge tube (Santa Cruz, cat. no. sc-200252) Cell passaging tool (Life Technologies, cat. no. 23181-010) Cell Scraper (Sarstedt, cat. no. 83.1830) CryoTube vials (Nunc, cat. no. 375418) P1000 Barrier Pipette Tips (Denville Scientific, cat. no. P1126) P200 Barrier Pipette Tips (Denville Scientific, cat. no. P1122) P20 Barrier Pipette Tips (Denville Scientific, cat. no. 1121) P10 Barrier Pipette Tips (Denville Scientific, cat. no. P1096-FR) Serological Pipette – 2 mL, individually wrapped (Santa Cruz, cat. no. sc-200276) Serological Pipette – 5 mL, individually wrapped (Santa Cruz, cat. no. sc-200279) Serological Pipette – 10 mL, individually wrapped (Santa Cruz, cat. no. sc-200281) Serological Pipette – 25 mL, individually wrapped (Santa Cruz, cat. no. sc-200283) Serological Pipette – 50 mL, individually wrapped (Santa Cruz, cat. no. sc-213234) Sterile Filter Systems, disposable (0.22 uM, PES) (Corning, cat. no. 431097) Steriflip-GP Filter, 50ml, 0.22μm (EMD Millipore, cat. no. EW-29969-24) ..

Isolation:

Article Title: Multiple myeloma–derived MMP-13 mediates osteoclast fusogenesis and osteolytic disease
Article Snippet: For human cell studies, BM mononuclear cells (1 × 105 cells/well) isolated from patients with MM were seeded in 96-well plates in α-MEM supplemented with 20% horse serum, 10 ng/ml human M-CSF (hM-CSF), and 50 ng/ml soluble human RANKL (hRANKL) (both from R & D Systems) in the absence or presence of rh–pro–MMP-13. .. The number of OCLs per well, the number of nuclei per OCL, and OCL size were quantified using an Olympus IX70 microscope equipped with a 20/0.40 numeric aperture objective lens and SPOT software (Diagnostic Instruments) or a Leica DMI 6000B fluorescence microscope equipped with an HCX PL S-APO 10×/NA 0.3 objective lens, coupled with a Leica DFC365 FX digital camera and operated by LAS AF 6.2 software (Leica Microsystems).

Transferring:

Article Title: The in vitro generation of lung and airway progenitor cells from human pluripotent stem cells
Article Snippet: .. Mini Microcentrifuge Motorized Leica DMI 6000B fluorescence microscope coupled with Leica DFC365 FX and DFC450 digital cameras and operated by LAS AF 6.2 software (Leica Microsystems GmbH, Wetzlar, Germany) Tabletop centrifuge (Eppendorf, Centrifuge 5810 R) Water bath set at 37°C (Fisher Scientific, IsoTemp 210) 1.7 mL Posi-Click Microcentrifuge tubes (Denville Scientific, cat. no. C2170) 6-well Ultra-Low Attachment plate (Corning, cat. no. 3471) 6-well tissue culture-treated plate (Falcon, cat. no. 353046) 24-well tissue culture-treated plate (Falcon, cat. no. 353047) 48-well tissue culture-treated plate (Falcon, cat. no. 353078) 10 cm2 tissue culture-treated plates (Falcon, cat. no. 353003) 15 mL polypropylene conical centrifuge tube (Santa Cruz, cat. no. sc-200249) 50 mL polypropylene conical centrifuge tube (Santa Cruz, cat. no. sc-200252) Cell passaging tool (Life Technologies, cat. no. 23181-010) Cell Scraper (Sarstedt, cat. no. 83.1830) CryoTube vials (Nunc, cat. no. 375418) P1000 Barrier Pipette Tips (Denville Scientific, cat. no. P1126) P200 Barrier Pipette Tips (Denville Scientific, cat. no. P1122) P20 Barrier Pipette Tips (Denville Scientific, cat. no. 1121) P10 Barrier Pipette Tips (Denville Scientific, cat. no. P1096-FR) Serological Pipette – 2 mL, individually wrapped (Santa Cruz, cat. no. sc-200276) Serological Pipette – 5 mL, individually wrapped (Santa Cruz, cat. no. sc-200279) Serological Pipette – 10 mL, individually wrapped (Santa Cruz, cat. no. sc-200281) Serological Pipette – 25 mL, individually wrapped (Santa Cruz, cat. no. sc-200283) Serological Pipette – 50 mL, individually wrapped (Santa Cruz, cat. no. sc-213234) Sterile Filter Systems, disposable (0.22 uM, PES) (Corning, cat. no. 431097) Steriflip-GP Filter, 50ml, 0.22μm (EMD Millipore, cat. no. EW-29969-24) ..

Microscopy:

Article Title: Highly efficient generation of airway and lung epithelial cells from human pluripotent stem cells
Article Snippet: .. Samples were visualized and imaged using motorized Leica DMI 6000B fluorescence microscope coupled with Leica DFC365 FX digital camera and operated by LAS AF 6.2 software (Leica Microsystems GmbH, Wetzlar, Germany). ..

Article Title: Conditional PDK1 ablation promotes epidermal and T cell-mediated dysfunctions leading to inflammatory skin disease
Article Snippet: .. Images were obtained using LAS AF 6.2 software on a motorized Leica DMI 6000B fluorescence microscope (Leica Microsystems GmbH, Wetzlar, Germany). .. T cell transfer and bone marrow reconstitution T cells were purified from spleen and peripheral lymph nodes of PDK1-CKO and -CHET mice by anti CD3ε microbeads (Miltenyi Biotec).

Article Title: Short-term high-fat feeding induces islet macrophage infiltration and β-cell replication independently of insulin resistance in mice
Article Snippet: .. Images were recorded using LAS AF 6.2 software on a motorized Leica DMI 600B fluorescence microscope (Leica Microsystems, Wetzlar, Germany). ..

Article Title: The in vitro generation of lung and airway progenitor cells from human pluripotent stem cells
Article Snippet: .. Mini Microcentrifuge Motorized Leica DMI 6000B fluorescence microscope coupled with Leica DFC365 FX and DFC450 digital cameras and operated by LAS AF 6.2 software (Leica Microsystems GmbH, Wetzlar, Germany) Tabletop centrifuge (Eppendorf, Centrifuge 5810 R) Water bath set at 37°C (Fisher Scientific, IsoTemp 210) 1.7 mL Posi-Click Microcentrifuge tubes (Denville Scientific, cat. no. C2170) 6-well Ultra-Low Attachment plate (Corning, cat. no. 3471) 6-well tissue culture-treated plate (Falcon, cat. no. 353046) 24-well tissue culture-treated plate (Falcon, cat. no. 353047) 48-well tissue culture-treated plate (Falcon, cat. no. 353078) 10 cm2 tissue culture-treated plates (Falcon, cat. no. 353003) 15 mL polypropylene conical centrifuge tube (Santa Cruz, cat. no. sc-200249) 50 mL polypropylene conical centrifuge tube (Santa Cruz, cat. no. sc-200252) Cell passaging tool (Life Technologies, cat. no. 23181-010) Cell Scraper (Sarstedt, cat. no. 83.1830) CryoTube vials (Nunc, cat. no. 375418) P1000 Barrier Pipette Tips (Denville Scientific, cat. no. P1126) P200 Barrier Pipette Tips (Denville Scientific, cat. no. P1122) P20 Barrier Pipette Tips (Denville Scientific, cat. no. 1121) P10 Barrier Pipette Tips (Denville Scientific, cat. no. P1096-FR) Serological Pipette – 2 mL, individually wrapped (Santa Cruz, cat. no. sc-200276) Serological Pipette – 5 mL, individually wrapped (Santa Cruz, cat. no. sc-200279) Serological Pipette – 10 mL, individually wrapped (Santa Cruz, cat. no. sc-200281) Serological Pipette – 25 mL, individually wrapped (Santa Cruz, cat. no. sc-200283) Serological Pipette – 50 mL, individually wrapped (Santa Cruz, cat. no. sc-213234) Sterile Filter Systems, disposable (0.22 uM, PES) (Corning, cat. no. 431097) Steriflip-GP Filter, 50ml, 0.22μm (EMD Millipore, cat. no. EW-29969-24) ..

Article Title: Multiple myeloma–derived MMP-13 mediates osteoclast fusogenesis and osteolytic disease
Article Snippet: .. The number of OCLs per well, the number of nuclei per OCL, and OCL size were quantified using an Olympus IX70 microscope equipped with a 20/0.40 numeric aperture objective lens and SPOT software (Diagnostic Instruments) or a Leica DMI 6000B fluorescence microscope equipped with an HCX PL S-APO 10×/NA 0.3 objective lens, coupled with a Leica DFC365 FX digital camera and operated by LAS AF 6.2 software (Leica Microsystems). .. Images of resorption lacunae were captured using an OMAX 40X-2000X Digital Microscope with ScopeImage 9.0 software (BioImager) or Magnafire 4.1 software (Optronics).

Purification:

Article Title: Multiple myeloma–derived MMP-13 mediates osteoclast fusogenesis and osteolytic disease
Article Snippet: To investigate MMP-13–induced ERK1/2 signaling in pre-OCLs, CD11b+ cells were purified by CD11b microbeads (Miltenyi Biotec) and cultured for 3 days with 10 ng/ml M-CSF and 50 ng/ml RANKL. .. The number of OCLs per well, the number of nuclei per OCL, and OCL size were quantified using an Olympus IX70 microscope equipped with a 20/0.40 numeric aperture objective lens and SPOT software (Diagnostic Instruments) or a Leica DMI 6000B fluorescence microscope equipped with an HCX PL S-APO 10×/NA 0.3 objective lens, coupled with a Leica DFC365 FX digital camera and operated by LAS AF 6.2 software (Leica Microsystems).

Mutagenesis:

Article Title: Multiple myeloma–derived MMP-13 mediates osteoclast fusogenesis and osteolytic disease
Article Snippet: After 4 hours of serum-free starvation, MMP-13 WT or the E223A mutant (200 ng/ml) was added for the durations indicated in prior to collection of lysates for Western blot analysis. .. The number of OCLs per well, the number of nuclei per OCL, and OCL size were quantified using an Olympus IX70 microscope equipped with a 20/0.40 numeric aperture objective lens and SPOT software (Diagnostic Instruments) or a Leica DMI 6000B fluorescence microscope equipped with an HCX PL S-APO 10×/NA 0.3 objective lens, coupled with a Leica DFC365 FX digital camera and operated by LAS AF 6.2 software (Leica Microsystems).

Incubation:

Article Title: Highly efficient generation of airway and lung epithelial cells from human pluripotent stem cells
Article Snippet: The cultures were then incubated with the corresponding secondary antibodies at 1:300 dilutions in staining/wash buffer at room temperature for 2hr, washed twice for 10 min and incubated with DAPI for 5 min at room temperature. .. Samples were visualized and imaged using motorized Leica DMI 6000B fluorescence microscope coupled with Leica DFC365 FX digital camera and operated by LAS AF 6.2 software (Leica Microsystems GmbH, Wetzlar, Germany).

Cell Culture:

Article Title: Multiple myeloma–derived MMP-13 mediates osteoclast fusogenesis and osteolytic disease
Article Snippet: To investigate MMP-13–induced ERK1/2 signaling in pre-OCLs, CD11b+ cells were purified by CD11b microbeads (Miltenyi Biotec) and cultured for 3 days with 10 ng/ml M-CSF and 50 ng/ml RANKL. .. The number of OCLs per well, the number of nuclei per OCL, and OCL size were quantified using an Olympus IX70 microscope equipped with a 20/0.40 numeric aperture objective lens and SPOT software (Diagnostic Instruments) or a Leica DMI 6000B fluorescence microscope equipped with an HCX PL S-APO 10×/NA 0.3 objective lens, coupled with a Leica DFC365 FX digital camera and operated by LAS AF 6.2 software (Leica Microsystems).

Staining:

Article Title: Highly efficient generation of airway and lung epithelial cells from human pluripotent stem cells
Article Snippet: Paragraph title: Immunofluorescence Staining ... Samples were visualized and imaged using motorized Leica DMI 6000B fluorescence microscope coupled with Leica DFC365 FX digital camera and operated by LAS AF 6.2 software (Leica Microsystems GmbH, Wetzlar, Germany).

Article Title: Conditional PDK1 ablation promotes epidermal and T cell-mediated dysfunctions leading to inflammatory skin disease
Article Snippet: Skin cryostat sections (5mm) were stained with antibodies specific for YFP (life technologies, Grand Island, NY), OX40 (BD Biosciences), filaggrin (Santacruz, Dallas, TX), involucrin (Covance, Princeton, NJ), loricrin, cytokeratin14, cytokeratin15, cytokeratin10 (Genetex, Irvine, CA) Ki67, CD4 (eBioscience), cleaved caspase 3 (Cellsignaling), and TSLP (R & D Systems, Minneapolis, MN), then stained with fluorescently-coupled secondary anti-rabbit or -rat IgG (Cellsignaling Technologies, Danvers, MA), or streptavidin-conjugated Cy5 (Biolegend). .. Images were obtained using LAS AF 6.2 software on a motorized Leica DMI 6000B fluorescence microscope (Leica Microsystems GmbH, Wetzlar, Germany).

Article Title: Short-term high-fat feeding induces islet macrophage infiltration and β-cell replication independently of insulin resistance in mice
Article Snippet: Paragraph title: Immunofluorescence staining of pancreatic tissue sections. ... Images were recorded using LAS AF 6.2 software on a motorized Leica DMI 600B fluorescence microscope (Leica Microsystems, Wetzlar, Germany).

Article Title: Multiple myeloma–derived MMP-13 mediates osteoclast fusogenesis and osteolytic disease
Article Snippet: Half-media changes were carried out every 3 days and OCL formation assessed by staining with mAb 23c6 (CD51/61), which was provided by Michael Horton (Rayne Institute, Bone and Mineral Center, London, United Kingdom) using a VECTASTAIN ABC-AP Kit (Vector Laboratories) ( ). .. The number of OCLs per well, the number of nuclei per OCL, and OCL size were quantified using an Olympus IX70 microscope equipped with a 20/0.40 numeric aperture objective lens and SPOT software (Diagnostic Instruments) or a Leica DMI 6000B fluorescence microscope equipped with an HCX PL S-APO 10×/NA 0.3 objective lens, coupled with a Leica DFC365 FX digital camera and operated by LAS AF 6.2 software (Leica Microsystems).

Marker:

Article Title: Short-term high-fat feeding induces islet macrophage infiltration and β-cell replication independently of insulin resistance in mice
Article Snippet: They were stained with primary antibodies specific for insulin (Dako), the MΦ marker F4/80 (Invitrogen), BrdU (BD Pharmingin), inducible nitric oxide synthase (iNOS, Abcam), stromal cell-derived factor 1 (SDF1, Abcam), and/or 8-oxo-2′-deoxyguanosine (8-OHdG, Santa Cruz Biotechnology,). .. Images were recorded using LAS AF 6.2 software on a motorized Leica DMI 600B fluorescence microscope (Leica Microsystems, Wetzlar, Germany).

Western Blot:

Article Title: Multiple myeloma–derived MMP-13 mediates osteoclast fusogenesis and osteolytic disease
Article Snippet: After 4 hours of serum-free starvation, MMP-13 WT or the E223A mutant (200 ng/ml) was added for the durations indicated in prior to collection of lysates for Western blot analysis. .. The number of OCLs per well, the number of nuclei per OCL, and OCL size were quantified using an Olympus IX70 microscope equipped with a 20/0.40 numeric aperture objective lens and SPOT software (Diagnostic Instruments) or a Leica DMI 6000B fluorescence microscope equipped with an HCX PL S-APO 10×/NA 0.3 objective lens, coupled with a Leica DFC365 FX digital camera and operated by LAS AF 6.2 software (Leica Microsystems).

Immunofluorescence:

Article Title: Highly efficient generation of airway and lung epithelial cells from human pluripotent stem cells
Article Snippet: Paragraph title: Immunofluorescence Staining ... Samples were visualized and imaged using motorized Leica DMI 6000B fluorescence microscope coupled with Leica DFC365 FX digital camera and operated by LAS AF 6.2 software (Leica Microsystems GmbH, Wetzlar, Germany).

Article Title: Conditional PDK1 ablation promotes epidermal and T cell-mediated dysfunctions leading to inflammatory skin disease
Article Snippet: Paragraph title: Immunofluorescence ... Images were obtained using LAS AF 6.2 software on a motorized Leica DMI 6000B fluorescence microscope (Leica Microsystems GmbH, Wetzlar, Germany).

Article Title: Short-term high-fat feeding induces islet macrophage infiltration and β-cell replication independently of insulin resistance in mice
Article Snippet: Paragraph title: Immunofluorescence staining of pancreatic tissue sections. ... Images were recorded using LAS AF 6.2 software on a motorized Leica DMI 600B fluorescence microscope (Leica Microsystems, Wetzlar, Germany).

Blocking Assay:

Article Title: Highly efficient generation of airway and lung epithelial cells from human pluripotent stem cells
Article Snippet: After blocking, all the triple, double or single staining (except “d25 Nkx2.1 (mouse)/p63(rabbit)/FOXA2(goat)” and “Tuj1/TTF-1/FOXA2”) were performed by incubating primary antibody(ies), according to the dilution factors indicated in in staining/wash buffer (5% fetal donkey serum in PBS) at 4°C overnight (minimum 12hr), followed by 3×10min wash. .. Samples were visualized and imaged using motorized Leica DMI 6000B fluorescence microscope coupled with Leica DFC365 FX digital camera and operated by LAS AF 6.2 software (Leica Microsystems GmbH, Wetzlar, Germany).

Software:

Article Title: Highly efficient generation of airway and lung epithelial cells from human pluripotent stem cells
Article Snippet: .. Samples were visualized and imaged using motorized Leica DMI 6000B fluorescence microscope coupled with Leica DFC365 FX digital camera and operated by LAS AF 6.2 software (Leica Microsystems GmbH, Wetzlar, Germany). ..

Article Title: Conditional PDK1 ablation promotes epidermal and T cell-mediated dysfunctions leading to inflammatory skin disease
Article Snippet: .. Images were obtained using LAS AF 6.2 software on a motorized Leica DMI 6000B fluorescence microscope (Leica Microsystems GmbH, Wetzlar, Germany). .. T cell transfer and bone marrow reconstitution T cells were purified from spleen and peripheral lymph nodes of PDK1-CKO and -CHET mice by anti CD3ε microbeads (Miltenyi Biotec).

Article Title: Short-term high-fat feeding induces islet macrophage infiltration and β-cell replication independently of insulin resistance in mice
Article Snippet: .. Images were recorded using LAS AF 6.2 software on a motorized Leica DMI 600B fluorescence microscope (Leica Microsystems, Wetzlar, Germany). ..

Article Title: The in vitro generation of lung and airway progenitor cells from human pluripotent stem cells
Article Snippet: .. Mini Microcentrifuge Motorized Leica DMI 6000B fluorescence microscope coupled with Leica DFC365 FX and DFC450 digital cameras and operated by LAS AF 6.2 software (Leica Microsystems GmbH, Wetzlar, Germany) Tabletop centrifuge (Eppendorf, Centrifuge 5810 R) Water bath set at 37°C (Fisher Scientific, IsoTemp 210) 1.7 mL Posi-Click Microcentrifuge tubes (Denville Scientific, cat. no. C2170) 6-well Ultra-Low Attachment plate (Corning, cat. no. 3471) 6-well tissue culture-treated plate (Falcon, cat. no. 353046) 24-well tissue culture-treated plate (Falcon, cat. no. 353047) 48-well tissue culture-treated plate (Falcon, cat. no. 353078) 10 cm2 tissue culture-treated plates (Falcon, cat. no. 353003) 15 mL polypropylene conical centrifuge tube (Santa Cruz, cat. no. sc-200249) 50 mL polypropylene conical centrifuge tube (Santa Cruz, cat. no. sc-200252) Cell passaging tool (Life Technologies, cat. no. 23181-010) Cell Scraper (Sarstedt, cat. no. 83.1830) CryoTube vials (Nunc, cat. no. 375418) P1000 Barrier Pipette Tips (Denville Scientific, cat. no. P1126) P200 Barrier Pipette Tips (Denville Scientific, cat. no. P1122) P20 Barrier Pipette Tips (Denville Scientific, cat. no. 1121) P10 Barrier Pipette Tips (Denville Scientific, cat. no. P1096-FR) Serological Pipette – 2 mL, individually wrapped (Santa Cruz, cat. no. sc-200276) Serological Pipette – 5 mL, individually wrapped (Santa Cruz, cat. no. sc-200279) Serological Pipette – 10 mL, individually wrapped (Santa Cruz, cat. no. sc-200281) Serological Pipette – 25 mL, individually wrapped (Santa Cruz, cat. no. sc-200283) Serological Pipette – 50 mL, individually wrapped (Santa Cruz, cat. no. sc-213234) Sterile Filter Systems, disposable (0.22 uM, PES) (Corning, cat. no. 431097) Steriflip-GP Filter, 50ml, 0.22μm (EMD Millipore, cat. no. EW-29969-24) ..

Article Title: Multiple myeloma–derived MMP-13 mediates osteoclast fusogenesis and osteolytic disease
Article Snippet: .. The number of OCLs per well, the number of nuclei per OCL, and OCL size were quantified using an Olympus IX70 microscope equipped with a 20/0.40 numeric aperture objective lens and SPOT software (Diagnostic Instruments) or a Leica DMI 6000B fluorescence microscope equipped with an HCX PL S-APO 10×/NA 0.3 objective lens, coupled with a Leica DFC365 FX digital camera and operated by LAS AF 6.2 software (Leica Microsystems). .. Images of resorption lacunae were captured using an OMAX 40X-2000X Digital Microscope with ScopeImage 9.0 software (BioImager) or Magnafire 4.1 software (Optronics).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Leica Microsystems leica las af software version 2 6 07266
    Leica Las Af Software Version 2 6 07266, supplied by Leica Microsystems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/leica las af software version 2 6 07266/product/Leica Microsystems
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    leica las af software version 2 6 07266 - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    94
    Leica Microsystems leica software tools line profile intensity sort roi
    Leica Software Tools Line Profile Intensity Sort Roi, supplied by Leica Microsystems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/leica software tools line profile intensity sort roi/product/Leica Microsystems
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    leica software tools line profile intensity sort roi - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    92
    Leica Microsystems v 2 3 6 software
    V 2 3 6 Software, supplied by Leica Microsystems, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/v 2 3 6 software/product/Leica Microsystems
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    v 2 3 6 software - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    94
    Leica Microsystems las af 3 0 dye separation software module
    Las Af 3 0 Dye Separation Software Module, supplied by Leica Microsystems, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/las af 3 0 dye separation software module/product/Leica Microsystems
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    las af 3 0 dye separation software module - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    Image Search Results