novablue e coli amplification strain  (Millipore)


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    Millipore novablue e coli amplification strain
    Novablue E Coli Amplification Strain, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/novablue e coli amplification strain/product/Millipore
    Average 79 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    novablue e coli amplification strain - by Bioz Stars, 2020-01
    79/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Immobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success
    Article Snippet: Paragraph title: High-throughput cloning procedure ... The annealing reaction was stopped by the addition of 1 µl 25 mM EDTA followed by a heat-shock transformation (10 min on ice, followed by 45 s at 315 K and then ice for 30 min) into NovaBlue E. coli amplification strain (EMD Biosciences, Gibbstown, New Jersey, USA).

    Amplification:

    Article Title: Immobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success
    Article Snippet: .. The annealing reaction was stopped by the addition of 1 µl 25 mM EDTA followed by a heat-shock transformation (10 min on ice, followed by 45 s at 315 K and then ice for 30 min) into NovaBlue E. coli amplification strain (EMD Biosciences, Gibbstown, New Jersey, USA). .. The LIC plasmid constructs were purified from the amplification host using a 96-well Turbo Miniprep kit (Qiagen, Valencia, California, USA).

    Agarose Gel Electrophoresis:

    Article Title: Immobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success
    Article Snippet: After PCR amplification, the entire 50 µl PCR reaction was run for 1.25 h at 150 V on a 1% TAE agarose gel with EtBr. .. The annealing reaction was stopped by the addition of 1 µl 25 mM EDTA followed by a heat-shock transformation (10 min on ice, followed by 45 s at 315 K and then ice for 30 min) into NovaBlue E. coli amplification strain (EMD Biosciences, Gibbstown, New Jersey, USA).

    High Throughput Screening Assay:

    Article Title: Immobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success
    Article Snippet: Paragraph title: High-throughput cloning procedure ... The annealing reaction was stopped by the addition of 1 µl 25 mM EDTA followed by a heat-shock transformation (10 min on ice, followed by 45 s at 315 K and then ice for 30 min) into NovaBlue E. coli amplification strain (EMD Biosciences, Gibbstown, New Jersey, USA).

    Construct:

    Article Title: Immobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success
    Article Snippet: The annealing reaction was stopped by the addition of 1 µl 25 mM EDTA followed by a heat-shock transformation (10 min on ice, followed by 45 s at 315 K and then ice for 30 min) into NovaBlue E. coli amplification strain (EMD Biosciences, Gibbstown, New Jersey, USA). .. The LIC plasmid constructs were purified from the amplification host using a 96-well Turbo Miniprep kit (Qiagen, Valencia, California, USA).

    Purification:

    Article Title: Immobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success
    Article Snippet: The PCR products were excised from the gel (after imaging and size verification) and purified using a 96-well gel-extraction kit (Qiagen, Valencia, California, USA). .. The annealing reaction was stopped by the addition of 1 µl 25 mM EDTA followed by a heat-shock transformation (10 min on ice, followed by 45 s at 315 K and then ice for 30 min) into NovaBlue E. coli amplification strain (EMD Biosciences, Gibbstown, New Jersey, USA).

    Polymerase Chain Reaction:

    Article Title: Immobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success
    Article Snippet: The PCR products were excised from the gel (after imaging and size verification) and purified using a 96-well gel-extraction kit (Qiagen, Valencia, California, USA). .. The annealing reaction was stopped by the addition of 1 µl 25 mM EDTA followed by a heat-shock transformation (10 min on ice, followed by 45 s at 315 K and then ice for 30 min) into NovaBlue E. coli amplification strain (EMD Biosciences, Gibbstown, New Jersey, USA).

    Incubation:

    Article Title: Immobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success
    Article Snippet: At this point, 2 µl of the T4-treated insert and 1 µl of treated vector were incubated together at ambient temperature, generally 293–295 K, for 5–30 min. .. The annealing reaction was stopped by the addition of 1 µl 25 mM EDTA followed by a heat-shock transformation (10 min on ice, followed by 45 s at 315 K and then ice for 30 min) into NovaBlue E. coli amplification strain (EMD Biosciences, Gibbstown, New Jersey, USA).

    Imaging:

    Article Title: Immobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success
    Article Snippet: The PCR products were excised from the gel (after imaging and size verification) and purified using a 96-well gel-extraction kit (Qiagen, Valencia, California, USA). .. The annealing reaction was stopped by the addition of 1 µl 25 mM EDTA followed by a heat-shock transformation (10 min on ice, followed by 45 s at 315 K and then ice for 30 min) into NovaBlue E. coli amplification strain (EMD Biosciences, Gibbstown, New Jersey, USA).

    Expressing:

    Article Title: Immobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success
    Article Snippet: The annealing reaction was stopped by the addition of 1 µl 25 mM EDTA followed by a heat-shock transformation (10 min on ice, followed by 45 s at 315 K and then ice for 30 min) into NovaBlue E. coli amplification strain (EMD Biosciences, Gibbstown, New Jersey, USA). .. The purified plasmids were then transformed into the expression host strain for expression screening.

    Sequencing:

    Article Title: Immobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success
    Article Snippet: The primers used to amplify the insert genes had an LIC sequence appended to their 5′ ends that was complementary to the restriction sites/LIC sequences in the vector. .. The annealing reaction was stopped by the addition of 1 µl 25 mM EDTA followed by a heat-shock transformation (10 min on ice, followed by 45 s at 315 K and then ice for 30 min) into NovaBlue E. coli amplification strain (EMD Biosciences, Gibbstown, New Jersey, USA).

    Gel Extraction:

    Article Title: Immobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success
    Article Snippet: The PCR products were excised from the gel (after imaging and size verification) and purified using a 96-well gel-extraction kit (Qiagen, Valencia, California, USA). .. The annealing reaction was stopped by the addition of 1 µl 25 mM EDTA followed by a heat-shock transformation (10 min on ice, followed by 45 s at 315 K and then ice for 30 min) into NovaBlue E. coli amplification strain (EMD Biosciences, Gibbstown, New Jersey, USA).

    Transformation Assay:

    Article Title: Immobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success
    Article Snippet: .. The annealing reaction was stopped by the addition of 1 µl 25 mM EDTA followed by a heat-shock transformation (10 min on ice, followed by 45 s at 315 K and then ice for 30 min) into NovaBlue E. coli amplification strain (EMD Biosciences, Gibbstown, New Jersey, USA). .. The LIC plasmid constructs were purified from the amplification host using a 96-well Turbo Miniprep kit (Qiagen, Valencia, California, USA).

    Plasmid Preparation:

    Article Title: Immobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success
    Article Snippet: At this point, 2 µl of the T4-treated insert and 1 µl of treated vector were incubated together at ambient temperature, generally 293–295 K, for 5–30 min. .. The annealing reaction was stopped by the addition of 1 µl 25 mM EDTA followed by a heat-shock transformation (10 min on ice, followed by 45 s at 315 K and then ice for 30 min) into NovaBlue E. coli amplification strain (EMD Biosciences, Gibbstown, New Jersey, USA).

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  • 79
    Millipore novablue e coli amplification strain
    Novablue E Coli Amplification Strain, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/novablue e coli amplification strain/product/Millipore
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    novablue e coli amplification strain - by Bioz Stars, 2020-01
    79/100 stars
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    Millipore competent e coli novablue cells
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    Millipore e coli strain novablue gigasingles
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