la taq tm kit  (TaKaRa)

 
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    Name:
    TaKaRa LA Taq DNA Polymerase
    Description:
    TaKaRa LA Taq DNA Polymerase combines Taq DNA polymerase and a DNA proofreading polymerase with 3 →5 exonuclease activity to enable PCR amplification of very long DNA templates long range PCR This mixture of enzymes allows for long and accurate LA PCR of targets from a variety of templates including genomic DNA LA Taq DNA polymerase is supplied with optimized LA PCR Buffer II with or without Mg2 and dNTPs
    Catalog Number:
    rr002a
    Price:
    None
    Size:
    125 Units
    Category:
    LA Taq DNA polymerase LA Taq products Long range PCR PCR
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    Structured Review

    TaKaRa la taq tm kit
    TaKaRa LA Taq DNA Polymerase combines Taq DNA polymerase and a DNA proofreading polymerase with 3 →5 exonuclease activity to enable PCR amplification of very long DNA templates long range PCR This mixture of enzymes allows for long and accurate LA PCR of targets from a variety of templates including genomic DNA LA Taq DNA polymerase is supplied with optimized LA PCR Buffer II with or without Mg2 and dNTPs
    https://www.bioz.com/result/la taq tm kit/product/TaKaRa
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    la taq tm kit - by Bioz Stars, 2020-10
    99/100 stars

    Related Products / Commonly Used Together

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Amplification and next generation sequencing of near full-length human enteroviruses for identification and characterisation from clinical samples
    Article Snippet: .. Amplification of Near Full-Length EV Genomes First round PCR was performed by adding cDNA (5 µL) to a reaction mix (50 µL) containing Takara LA Buffer (1X), dNTPs (100 µM each), forward primer vir24 (0.2 µM), reverse primer vir20 (0.2 µM), nuclease-free water (29.5 µL) and Takara LA DNA polymerase (2.5 U). .. PCR was performed at 94 °C for 1 min, 30 cycles at 94 °C for 30 s, 60 °C for 30 s, and 72 °C for 8 min, followed by a final extension at 72 °C for 5 min. Cycling conditions for samples with high GC/secondary structures were selected, as recommended in the manufacturer’s protocol.

    Article Title: Amplification and next generation sequencing of near full-length human enteroviruses for identification and characterisation from clinical samples
    Article Snippet: .. First round PCR was performed by adding cDNA (5 µL) to a reaction mix (50 µL) containing Takara LA Buffer (1X), dNTPs (100 µM each), forward primer vir24 (0.2 µM), reverse primer vir20 (0.2 µM), nuclease-free water (29.5 µL) and Takara LA DNA polymerase (2.5 U). .. PCR was performed at 94 °C for 1 min, 30 cycles at 94 °C for 30 s, 60 °C for 30 s, and 72 °C for 8 min, followed by a final extension at 72 °C for 5 min. Cycling conditions for samples with high GC/secondary structures were selected, as recommended in the manufacturer’s protocol.

    Article Title: A Multiplex PCR Detection Assay for the Identification of Clinically Relevant Anaplasma Species in Field Blood Samples
    Article Snippet: .. PCR conditions were investigated including the variation of the annealing temperature from 57 to 64°C, the concentration of primer sets from 0.08 to 0.56 μM, the concentration of La Taq DNA polymerase (TaKaRa, Dalian, China) from 0.75 to 1.75 U, and the concentration of PCR buffer (10×) and the dNTPs (2.5 mM) from 1.5 to 3 μL and 2 to 6 μL, respectively. ..

    Article Title: A Multiplex PCR Detection Assay for the Identification of Clinically Relevant Anaplasma Species in Field Blood Samples
    Article Snippet: .. Optimization of the Multiplex PCR After optimization, the optimum multiplex PCR assay was performed in a final volume of 25 μL, containing 2.5 μL of 10× PCR La buffer, 4 μL of dNTPs at 2.5 mM, 1.25 U of La Taq DNA polymerase, 0.32 μM of each primer, and 2 μL of the DNA template. ..

    Article Title: Detection of virulence factors of South African Lactococcus garvieae isolated from rainbow trout, Oncorhynchus mykiss (Walbaum)
    Article Snippet: .. The L. garvieae EPS synthesis gene cluster, as described in L. garvieae Lg2 (Miyauchi et al. ), was amplified in isolates A1–A3, A5, A6, A11 and A12 using the TaKaRa LA PCR Kit (TaKaRa Bio Inc. #RR002A, Kyoto, Japan). .. Amplicons were visualised on a 1% agarose gel.

    Concentration Assay:

    Article Title: A Multiplex PCR Detection Assay for the Identification of Clinically Relevant Anaplasma Species in Field Blood Samples
    Article Snippet: .. PCR conditions were investigated including the variation of the annealing temperature from 57 to 64°C, the concentration of primer sets from 0.08 to 0.56 μM, the concentration of La Taq DNA polymerase (TaKaRa, Dalian, China) from 0.75 to 1.75 U, and the concentration of PCR buffer (10×) and the dNTPs (2.5 mM) from 1.5 to 3 μL and 2 to 6 μL, respectively. ..

    Multiplex Assay:

    Article Title: A Multiplex PCR Detection Assay for the Identification of Clinically Relevant Anaplasma Species in Field Blood Samples
    Article Snippet: .. Optimization of the Multiplex PCR After optimization, the optimum multiplex PCR assay was performed in a final volume of 25 μL, containing 2.5 μL of 10× PCR La buffer, 4 μL of dNTPs at 2.5 mM, 1.25 U of La Taq DNA polymerase, 0.32 μM of each primer, and 2 μL of the DNA template. ..

    Amplification:

    Article Title: Amplification and next generation sequencing of near full-length human enteroviruses for identification and characterisation from clinical samples
    Article Snippet: .. Amplification of Near Full-Length EV Genomes First round PCR was performed by adding cDNA (5 µL) to a reaction mix (50 µL) containing Takara LA Buffer (1X), dNTPs (100 µM each), forward primer vir24 (0.2 µM), reverse primer vir20 (0.2 µM), nuclease-free water (29.5 µL) and Takara LA DNA polymerase (2.5 U). .. PCR was performed at 94 °C for 1 min, 30 cycles at 94 °C for 30 s, 60 °C for 30 s, and 72 °C for 8 min, followed by a final extension at 72 °C for 5 min. Cycling conditions for samples with high GC/secondary structures were selected, as recommended in the manufacturer’s protocol.

    Article Title: Simultaneous Use of MutS and RecA for Suppression of Nonspecific Amplification during PCR
    Article Snippet: .. The 423-base pair (bp) region of the ttha1806 gene was amplified by Takara LA Taq using T. thermophilus HB8 genomic DNA as the template. .. Without ttMutS and ttRecA, 1000- to 5000-bp DNA fragments were amplified nonspecifically ( ). ttMutS showed a significant suppressing effect on the nonspecific amplifications in the presence of 0 to 0.4 mM ATP ( ).

    Article Title: Detection of virulence factors of South African Lactococcus garvieae isolated from rainbow trout, Oncorhynchus mykiss (Walbaum)
    Article Snippet: .. The L. garvieae EPS synthesis gene cluster, as described in L. garvieae Lg2 (Miyauchi et al. ), was amplified in isolates A1–A3, A5, A6, A11 and A12 using the TaKaRa LA PCR Kit (TaKaRa Bio Inc. #RR002A, Kyoto, Japan). .. Amplicons were visualised on a 1% agarose gel.

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  • 99
    TaKaRa la taq tm kit
    La Taq Tm Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/la taq tm kit/product/TaKaRa
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    la taq tm kit - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

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    primerscript tm pcr kit - by Bioz Stars, 2020-10
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