l arginine hydrochloride  (Millipore)


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    Name:
    L Arginine hydrochloride
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    Millipore l arginine hydrochloride
    Representative tracing of skin blood flow (%CVCmax) during local heating in the control and ascorbate-treated microdialysis sites of one subject following non-dairy dietary sodium (panel A; pretzel, 1120mg Na) and dairy-cheese sodium (panel B, cheddar cheese, 1120mg Na) consumption. The difference between the local heating plateau and the post-N G <t>-nitro-L-arginine</t> methyl ester (L-NAME) plateau indicates the vasodilation attributed to the production of NO by eNOS (%NO-dependent dilation).

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    Images

    1) Product Images from "Dairy cheese consumption ameliorates single-meal sodium-induced cutaneous microvascular dysfunction by reducing ascorbate-sensitive oxidants in healthy older adults"

    Article Title: Dairy cheese consumption ameliorates single-meal sodium-induced cutaneous microvascular dysfunction by reducing ascorbate-sensitive oxidants in healthy older adults

    Journal: The British journal of nutrition

    doi: 10.1017/S0007114516002579

    Representative tracing of skin blood flow (%CVCmax) during local heating in the control and ascorbate-treated microdialysis sites of one subject following non-dairy dietary sodium (panel A; pretzel, 1120mg Na) and dairy-cheese sodium (panel B, cheddar cheese, 1120mg Na) consumption. The difference between the local heating plateau and the post-N G -nitro-L-arginine methyl ester (L-NAME) plateau indicates the vasodilation attributed to the production of NO by eNOS (%NO-dependent dilation).
    Figure Legend Snippet: Representative tracing of skin blood flow (%CVCmax) during local heating in the control and ascorbate-treated microdialysis sites of one subject following non-dairy dietary sodium (panel A; pretzel, 1120mg Na) and dairy-cheese sodium (panel B, cheddar cheese, 1120mg Na) consumption. The difference between the local heating plateau and the post-N G -nitro-L-arginine methyl ester (L-NAME) plateau indicates the vasodilation attributed to the production of NO by eNOS (%NO-dependent dilation).

    Techniques Used: Flow Cytometry

    2) Product Images from "Macrophage regulation of B cell proliferation"

    Article Title: Macrophage regulation of B cell proliferation

    Journal: Cellular immunology

    doi: 10.1016/j.cellimm.2017.02.002

    Regulation of the PerC B cell response to BCR ligation by exogenous cytokines or prostaglandin C57BL/6J PerC cells were cultured with αCD3 or αIgM +/− the inducible nitric oxide synthase (iNOS) inhibitor N G -monomethyl_L-arginine (1-MA), the indoleamine 2,3 dioxygenase inhibitor (IDO) 1 methyl-tryptophan (1-MT), the arginase (ARG) inhibitor N-ω-hydroxy-nor L arginine (1-NA), the cyclooxygenase (COX) inhibitor indomethacin (INDO), αIFNγ, αIL-10, and/or αIL-4 (A). The exogenous cytokines IL-4, IL-10, or IL-13 +/− indomethacin were added to C57BL/6J PerC cells cultured with αIgM (B). C57BL/6J and BALB/c PerC cells were cultured with αIgM and exogenous cytokines (C).
    Figure Legend Snippet: Regulation of the PerC B cell response to BCR ligation by exogenous cytokines or prostaglandin C57BL/6J PerC cells were cultured with αCD3 or αIgM +/− the inducible nitric oxide synthase (iNOS) inhibitor N G -monomethyl_L-arginine (1-MA), the indoleamine 2,3 dioxygenase inhibitor (IDO) 1 methyl-tryptophan (1-MT), the arginase (ARG) inhibitor N-ω-hydroxy-nor L arginine (1-NA), the cyclooxygenase (COX) inhibitor indomethacin (INDO), αIFNγ, αIL-10, and/or αIL-4 (A). The exogenous cytokines IL-4, IL-10, or IL-13 +/− indomethacin were added to C57BL/6J PerC cells cultured with αIgM (B). C57BL/6J and BALB/c PerC cells were cultured with αIgM and exogenous cytokines (C).

    Techniques Used: Ligation, Cell Culture

    3) Product Images from "Amino Acids Regulate Transgene Expression in MDCK Cells"

    Article Title: Amino Acids Regulate Transgene Expression in MDCK Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0096823

    Effect of L-arginine metabolism on transgene expression. A: Schematic overview of some mammalian arginine metabolic pathways. ARG: arginase; NO: nitric oxide; NOS: nitric oxide synthase; ODC: ornithine decarboxylase; OTC: ornithine transcarbamylase. B–D: Mycoplasma-infected B 0 AT1-TMEM27 overexpressing MDCK cells were cultivated in arginine-free DMEM supplemented with 45 or 720 µM arginine in the presence or absence of the indicated concentrations of inhibitors of the following enzymes: (B) nitric oxide synthase (N G -nitro-L-arginine methyl ester, L-NAME); (C) ornithine decarboxylase (α-difluoromethylornithine, DFMO) and (D) arginase (Nw-Hydroxy-nor-L-arginine, nor-NOHA). E–F: Mycoplasma-infected B 0 AT1-TMEM27 overexpressing MDCK cells were cultivated in arginine-free DMEM supplemented with 45 µM arginine in the absence (negative control, CTR −) or presence of (E) the indicated metabolites or (F) a nitric oxide donor (sodium nitroprusside, SNP). Positive control (CTR +) represents cells treated with 720 µM arginine. B 0 AT1 expression was analyzed by Western blotting and intensity of the immunoreactive bands was quantified, standardized to β-actin and normalized to 45 µM arginine. Representative Western blotting images are shown. Data are represented as mean ± SEM (n = 3). Groups were compared by one-way ANOVA followed by Dunnett post-test; *** p≤0.001 ** p≤0.01.
    Figure Legend Snippet: Effect of L-arginine metabolism on transgene expression. A: Schematic overview of some mammalian arginine metabolic pathways. ARG: arginase; NO: nitric oxide; NOS: nitric oxide synthase; ODC: ornithine decarboxylase; OTC: ornithine transcarbamylase. B–D: Mycoplasma-infected B 0 AT1-TMEM27 overexpressing MDCK cells were cultivated in arginine-free DMEM supplemented with 45 or 720 µM arginine in the presence or absence of the indicated concentrations of inhibitors of the following enzymes: (B) nitric oxide synthase (N G -nitro-L-arginine methyl ester, L-NAME); (C) ornithine decarboxylase (α-difluoromethylornithine, DFMO) and (D) arginase (Nw-Hydroxy-nor-L-arginine, nor-NOHA). E–F: Mycoplasma-infected B 0 AT1-TMEM27 overexpressing MDCK cells were cultivated in arginine-free DMEM supplemented with 45 µM arginine in the absence (negative control, CTR −) or presence of (E) the indicated metabolites or (F) a nitric oxide donor (sodium nitroprusside, SNP). Positive control (CTR +) represents cells treated with 720 µM arginine. B 0 AT1 expression was analyzed by Western blotting and intensity of the immunoreactive bands was quantified, standardized to β-actin and normalized to 45 µM arginine. Representative Western blotting images are shown. Data are represented as mean ± SEM (n = 3). Groups were compared by one-way ANOVA followed by Dunnett post-test; *** p≤0.001 ** p≤0.01.

    Techniques Used: Expressing, Infection, Negative Control, Positive Control, Western Blot

    4) Product Images from "First evaluation of real-time nitric oxide changes in the coronary circulation in patients with non-ischaemic dilated cardiomyopathy using a catheter-type sensor"

    Article Title: First evaluation of real-time nitric oxide changes in the coronary circulation in patients with non-ischaemic dilated cardiomyopathy using a catheter-type sensor

    Journal: European Heart Journal

    doi: 10.1093/eurheartj/ehq156

    Study protocol. First, there were three 2-min infusions of acetylcholine (ACh) at 0.15, 1.5, and 15 µg/min via the infusion catheter positioned in the left anterior descending coronary artery. After a 5-min infusion of N G -monomethyl- l -arginine ( l -NMMA) at 40 µmol/min, ACh was infused at the three concentrations as before. Coronary arteriograms were recorded after each injection of ACh and l -NMMA. The coronary flow velocity of the left anterior descending coronary artery was continuously monitored during this protocol.
    Figure Legend Snippet: Study protocol. First, there were three 2-min infusions of acetylcholine (ACh) at 0.15, 1.5, and 15 µg/min via the infusion catheter positioned in the left anterior descending coronary artery. After a 5-min infusion of N G -monomethyl- l -arginine ( l -NMMA) at 40 µmol/min, ACh was infused at the three concentrations as before. Coronary arteriograms were recorded after each injection of ACh and l -NMMA. The coronary flow velocity of the left anterior descending coronary artery was continuously monitored during this protocol.

    Techniques Used: Injection, Flow Cytometry

    5) Product Images from "Acute dairy milk ingestion does not improve nitric oxide-dependent vasodilation in the cutaneous microcirculation1"

    Article Title: Acute dairy milk ingestion does not improve nitric oxide-dependent vasodilation in the cutaneous microcirculation1

    Journal: The British journal of nutrition

    doi: 10.1017/S0007114516001835

    Representative tracing of the local heating response in one subject. The arrow denotes the decrease in skin blood flow with NOS-inhibition. L-NAME, N G -nitro-L-arginine methyl ester.
    Figure Legend Snippet: Representative tracing of the local heating response in one subject. The arrow denotes the decrease in skin blood flow with NOS-inhibition. L-NAME, N G -nitro-L-arginine methyl ester.

    Techniques Used: Flow Cytometry, Inhibition

    6) Product Images from "Modulation of Innate Antigen-Presenting Cell Function by Pre-patent Schistosome Infection"

    Article Title: Modulation of Innate Antigen-Presenting Cell Function by Pre-patent Schistosome Infection

    Journal: PLoS Neglected Tropical Diseases

    doi: 10.1371/journal.pntd.0002136

    Impairment of T cell stimulation by pre-patent schistosome infection is independent of arginase, NOS, IL-10, PD-L1 and TGF-β. A, Splenocytes isolated from 4 week S. mansoni -infected or non-infected RAG-1 −/− mice co-cultured with CFSE-stained CD4 + T cells isolated from non-infected OT-II/RAG-1 −/− mice for 72 hrs with OVA peptide stimulation in the presence of Nor-NOHA, L-NMMA, anti-IL-10R or anti-PD-L1. Cells recovered from culture were stained for surface markers then analyzed for CD4 + T cell proliferation by flow cytometry. Proliferation Index was calculated. Horizontal bars represent mean of 5 independent mice. B, In a replicate, independent experiment, cells were isolated as in (A) and cultured in the presence of OVA peptide stimulation with anti-TGF-β1, β2, β3 antibody (B) or recombinant IL-2 (C) and. CD4 + T cell proliferation was analyzed by flow cytometry. Horizontal bars represent mean of 5 independent mice. Data are representative of at least 3 independent experiments. Nor-NOHA, N ω -hydroxy-nor-Arginine; L-NMMA, L-N G -monomethyl Arginine citrate; αIL-10R, anti-IL-10 receptor monoclonal antibody; αPD-L1, anti-PD-L1 monoclonal antibody; αTGF-β, anti-TGF-β1, β2, β3 monoclonal antibody.
    Figure Legend Snippet: Impairment of T cell stimulation by pre-patent schistosome infection is independent of arginase, NOS, IL-10, PD-L1 and TGF-β. A, Splenocytes isolated from 4 week S. mansoni -infected or non-infected RAG-1 −/− mice co-cultured with CFSE-stained CD4 + T cells isolated from non-infected OT-II/RAG-1 −/− mice for 72 hrs with OVA peptide stimulation in the presence of Nor-NOHA, L-NMMA, anti-IL-10R or anti-PD-L1. Cells recovered from culture were stained for surface markers then analyzed for CD4 + T cell proliferation by flow cytometry. Proliferation Index was calculated. Horizontal bars represent mean of 5 independent mice. B, In a replicate, independent experiment, cells were isolated as in (A) and cultured in the presence of OVA peptide stimulation with anti-TGF-β1, β2, β3 antibody (B) or recombinant IL-2 (C) and. CD4 + T cell proliferation was analyzed by flow cytometry. Horizontal bars represent mean of 5 independent mice. Data are representative of at least 3 independent experiments. Nor-NOHA, N ω -hydroxy-nor-Arginine; L-NMMA, L-N G -monomethyl Arginine citrate; αIL-10R, anti-IL-10 receptor monoclonal antibody; αPD-L1, anti-PD-L1 monoclonal antibody; αTGF-β, anti-TGF-β1, β2, β3 monoclonal antibody.

    Techniques Used: Cell Stimulation, Infection, Isolation, Mouse Assay, Cell Culture, Staining, Flow Cytometry, Cytometry, Recombinant

    7) Product Images from "Korean red ginseng inhibits arginase and contributes to endotheliumdependent vasorelaxation through endothelial nitric oxide synthase coupling"

    Article Title: Korean red ginseng inhibits arginase and contributes to endotheliumdependent vasorelaxation through endothelial nitric oxide synthase coupling

    Journal: Journal of Ginseng Research

    doi: 10.5142/jgr.2013.37.64

    Korean red ginseng water extract (KG-WE) induces nitric oxide-dependent vessel relaxation. (A) KG-WE treatment induces the relaxation of vessels pre-constricted by U46619 (10 -8 mol/L). This was prevented by incubation with the nitric oxide synthase inhibitor, N G -nitro-L-arginine methyl ester (L-NAME, 10 -5 mol/L) (A, * vs. U46619 without L-NAME, p
    Figure Legend Snippet: Korean red ginseng water extract (KG-WE) induces nitric oxide-dependent vessel relaxation. (A) KG-WE treatment induces the relaxation of vessels pre-constricted by U46619 (10 -8 mol/L). This was prevented by incubation with the nitric oxide synthase inhibitor, N G -nitro-L-arginine methyl ester (L-NAME, 10 -5 mol/L) (A, * vs. U46619 without L-NAME, p

    Techniques Used: Incubation

    8) Product Images from "Arginase II Contributes to the Ca2+/CaMKII/eNOS Axis by Regulating Ca2+ Concentration Between the Cytosol and Mitochondria in a p32‐Dependent Manner"

    Article Title: Arginase II Contributes to the Ca2+/CaMKII/eNOS Axis by Regulating Ca2+ Concentration Between the Cytosol and Mitochondria in a p32‐Dependent Manner

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    doi: 10.1161/JAHA.118.009579

    sip32‐induced NO ‐dependent vasorelaxation in the aortas of WT mice. A, Aortic segments from WT mice were incubated with si RNA (final concentration,100 nmol/L) for 24 hours. p32 level was detected using Western blot analysis after mitochondrial fractionation (n=4 independent experiments from 4 mice). B, Aortas of WT mice were treated with si RNA , and proteins phosphorylation was analyzed using Western blotting (n=3 independent experiments from 3 mice). NO production (C) and ROS generation (D) were measured as time‐dependent changes in DAF (4‐amino‐5‐methylamino‐2',7'‐difluorofluorescein) and DHE (dihydroethidine) fluorescence intensities, respectively. The slopes were defined as the change in fluorescence intensity, and relative slopes are presented as a bar graph. si RNA and ABH (2(S)‐amino‐6‐boronohexanoic acid) were incubated with aortas for 24 hours. NG‐nitro‐L‐arginine methyl ester (100 μmol/L) was used as a control (n=8 aortic segments from 4 mice). Endothelium‐dependent vasorelaxation responses to A (E), endothelium‐independent relaxation responses to SNP (F), and contractile responses to PE (G) were determined using si RNA ‐treated aortas (n=8 aortas from 4 mice). NO indicates nitric oxide; ROS, reactive oxygen species; siRNA, small interfering RNA; WT, wild‐type. *vs untreated, P
    Figure Legend Snippet: sip32‐induced NO ‐dependent vasorelaxation in the aortas of WT mice. A, Aortic segments from WT mice were incubated with si RNA (final concentration,100 nmol/L) for 24 hours. p32 level was detected using Western blot analysis after mitochondrial fractionation (n=4 independent experiments from 4 mice). B, Aortas of WT mice were treated with si RNA , and proteins phosphorylation was analyzed using Western blotting (n=3 independent experiments from 3 mice). NO production (C) and ROS generation (D) were measured as time‐dependent changes in DAF (4‐amino‐5‐methylamino‐2',7'‐difluorofluorescein) and DHE (dihydroethidine) fluorescence intensities, respectively. The slopes were defined as the change in fluorescence intensity, and relative slopes are presented as a bar graph. si RNA and ABH (2(S)‐amino‐6‐boronohexanoic acid) were incubated with aortas for 24 hours. NG‐nitro‐L‐arginine methyl ester (100 μmol/L) was used as a control (n=8 aortic segments from 4 mice). Endothelium‐dependent vasorelaxation responses to A (E), endothelium‐independent relaxation responses to SNP (F), and contractile responses to PE (G) were determined using si RNA ‐treated aortas (n=8 aortas from 4 mice). NO indicates nitric oxide; ROS, reactive oxygen species; siRNA, small interfering RNA; WT, wild‐type. *vs untreated, P

    Techniques Used: Mouse Assay, Incubation, Concentration Assay, Western Blot, Fractionation, Fluorescence, Small Interfering RNA

    9) Product Images from "Cinnamyl alcohol attenuates vasoconstriction by activation of K+ channels via NO-cGMP-protein kinase G pathway and inhibition of Rho-kinase"

    Article Title: Cinnamyl alcohol attenuates vasoconstriction by activation of K+ channels via NO-cGMP-protein kinase G pathway and inhibition of Rho-kinase

    Journal: Experimental & Molecular Medicine

    doi: 10.3858/emm.2012.44.12.083

    Endothelium and NO dependency of vasodilatory effects of CAL on the PE-induced contraction of rat thoracic aorta. (A) Vasodilatory effect of CAL in the presence (○) and absence (■) of endothelium in rat aorta. (B) NO-dependent vasodilatory effect of CAL in response to pretreatment with N G -nitro- L -arginine methyl ester ( L -NAME; 10 -4  M; ■). (C) sGC-dependent vasodilatory effect of CAL in response to pretreatment with methylene blue (MB; 10 -5  M; ■) and 1 H-[1,2,4]-oxadiazolole-[4,3-a] quinoxalin-10-one (ODQ; 10 -6  M; □). (D) pGC-dependent vasodilatory effect of CAL in response to pretreatment with atrial natriuretic peptide (ANP; 10 -8  and 10 -9  M). Relaxation values are indicated as the percentage of decrease in maximal tension in response to PE. Each marker represents the mean ± S.D. from at least three independent experiments.  * P
    Figure Legend Snippet: Endothelium and NO dependency of vasodilatory effects of CAL on the PE-induced contraction of rat thoracic aorta. (A) Vasodilatory effect of CAL in the presence (○) and absence (■) of endothelium in rat aorta. (B) NO-dependent vasodilatory effect of CAL in response to pretreatment with N G -nitro- L -arginine methyl ester ( L -NAME; 10 -4 M; ■). (C) sGC-dependent vasodilatory effect of CAL in response to pretreatment with methylene blue (MB; 10 -5 M; ■) and 1 H-[1,2,4]-oxadiazolole-[4,3-a] quinoxalin-10-one (ODQ; 10 -6 M; □). (D) pGC-dependent vasodilatory effect of CAL in response to pretreatment with atrial natriuretic peptide (ANP; 10 -8 and 10 -9 M). Relaxation values are indicated as the percentage of decrease in maximal tension in response to PE. Each marker represents the mean ± S.D. from at least three independent experiments. * P

    Techniques Used: Pyrolysis Gas Chromatography, Aqueous Normal-phase Chromatography, Marker

    10) Product Images from "Macrophage regulation of B cell proliferation"

    Article Title: Macrophage regulation of B cell proliferation

    Journal: Cellular immunology

    doi: 10.1016/j.cellimm.2017.02.002

    Regulation of the PerC B cell response to BCR ligation by exogenous cytokines or prostaglandin C57BL/6J PerC cells were cultured with αCD3 or αIgM +/− the inducible nitric oxide synthase (iNOS) inhibitor N G -monomethyl_L-arginine (1-MA), the indoleamine 2,3 dioxygenase inhibitor (IDO) 1 methyl-tryptophan (1-MT), the arginase (ARG) inhibitor N-ω-hydroxy-nor L arginine (1-NA), the cyclooxygenase (COX) inhibitor indomethacin (INDO), αIFNγ, αIL-10, and/or αIL-4 (A). The exogenous cytokines IL-4, IL-10, or IL-13 +/− indomethacin were added to C57BL/6J PerC cells cultured with αIgM (B). C57BL/6J and BALB/c PerC cells were cultured with αIgM and exogenous cytokines (C).
    Figure Legend Snippet: Regulation of the PerC B cell response to BCR ligation by exogenous cytokines or prostaglandin C57BL/6J PerC cells were cultured with αCD3 or αIgM +/− the inducible nitric oxide synthase (iNOS) inhibitor N G -monomethyl_L-arginine (1-MA), the indoleamine 2,3 dioxygenase inhibitor (IDO) 1 methyl-tryptophan (1-MT), the arginase (ARG) inhibitor N-ω-hydroxy-nor L arginine (1-NA), the cyclooxygenase (COX) inhibitor indomethacin (INDO), αIFNγ, αIL-10, and/or αIL-4 (A). The exogenous cytokines IL-4, IL-10, or IL-13 +/− indomethacin were added to C57BL/6J PerC cells cultured with αIgM (B). C57BL/6J and BALB/c PerC cells were cultured with αIgM and exogenous cytokines (C).

    Techniques Used: Ligation, Cell Culture

    11) Product Images from "Oral sapropterin acutely augments reflex vasodilation in aged human skin through nitric oxide-dependent mechanisms"

    Article Title: Oral sapropterin acutely augments reflex vasodilation in aged human skin through nitric oxide-dependent mechanisms

    Journal: Journal of Applied Physiology

    doi: 10.1152/japplphysiol.00481.2013

    Group means ± SE of vasodilation response [percent of maximal vasodilation (%CVC max )] to increased core temperature (ΔT or ) at baseline (T or = 0.0) and during whole body heating in Ringer's control ( A ), BH 4 -perfused ( B ), and N G -nitro- l
    Figure Legend Snippet: Group means ± SE of vasodilation response [percent of maximal vasodilation (%CVC max )] to increased core temperature (ΔT or ) at baseline (T or = 0.0) and during whole body heating in Ringer's control ( A ), BH 4 -perfused ( B ), and N G -nitro- l

    Techniques Used:

    12) Product Images from "Cannabinoid Regulation of Nitric Oxide Synthase I (nNOS) in Neuronal Cells"

    Article Title: Cannabinoid Regulation of Nitric Oxide Synthase I (nNOS) in Neuronal Cells

    Journal: Journal of Neuroimmune Pharmacology

    doi: 10.1007/s11481-009-9153-7

    Endocannabinoid-stimulated NO accumulation antagonized by a NOS inhibitor. a N18TG2 cells were treated with a methanandamide (MetAEA) alone (1 μM) or b following pretreatment with NOS inhibitor N G -nitro- l -arginine ( l- NNA ; 1 μM; bar = 100 μm). b Quantitation of effects of NOS inhibitor l- NNA and its inactive enantiomer N G -nitro- d -arginine ( d -NNA ; 1 μM) on methanandamide-stimulated NO accumulation in N18TG2 cells. Data are presented as the DAF/DAPI fluorescence intensity ratio (mean ± SEM from N = 3 independent experiments). Values significantly different from those of MetAEA-stimulated cells (* p
    Figure Legend Snippet: Endocannabinoid-stimulated NO accumulation antagonized by a NOS inhibitor. a N18TG2 cells were treated with a methanandamide (MetAEA) alone (1 μM) or b following pretreatment with NOS inhibitor N G -nitro- l -arginine ( l- NNA ; 1 μM; bar = 100 μm). b Quantitation of effects of NOS inhibitor l- NNA and its inactive enantiomer N G -nitro- d -arginine ( d -NNA ; 1 μM) on methanandamide-stimulated NO accumulation in N18TG2 cells. Data are presented as the DAF/DAPI fluorescence intensity ratio (mean ± SEM from N = 3 independent experiments). Values significantly different from those of MetAEA-stimulated cells (* p

    Techniques Used: Quantitation Assay, Fluorescence

    13) Product Images from "Hydroxyurea Generates Nitric Oxide in Human Erythroid Cells: Mechanisms for ?-Globin Gene Activation"

    Article Title: Hydroxyurea Generates Nitric Oxide in Human Erythroid Cells: Mechanisms for ?-Globin Gene Activation

    Journal: Experimental biology and medicine (Maywood, N.J.)

    doi: 10.3181/0811-RM-339

    Mechanisms for γ-globin gene activation via NO signaling. Shown is a model of how hydroxyurea (HU) and detanonoate (DE) act as nitric oxide (NO) donors. The level of γ-globin transcription is increased by cyclic guanosine monophosphate (cGMP) signaling triggered by the conversion of guanosine triphosphate (GTP) to cGMP by soluble guanosine cyclase (sGC). Nitric oxide synthase (NOS) inhibitors N G -monomethyl-L-arginine (L-NMMA), N G -nitro-L-arginine methyl ester (L-NAME), and N G )oxadiazolo(4,3-a)quinoxalin-1-one (ODQ).
    Figure Legend Snippet: Mechanisms for γ-globin gene activation via NO signaling. Shown is a model of how hydroxyurea (HU) and detanonoate (DE) act as nitric oxide (NO) donors. The level of γ-globin transcription is increased by cyclic guanosine monophosphate (cGMP) signaling triggered by the conversion of guanosine triphosphate (GTP) to cGMP by soluble guanosine cyclase (sGC). Nitric oxide synthase (NOS) inhibitors N G -monomethyl-L-arginine (L-NMMA), N G -nitro-L-arginine methyl ester (L-NAME), and N G )oxadiazolo(4,3-a)quinoxalin-1-one (ODQ).

    Techniques Used: Activation Assay, Activated Clotting Time Assay

    14) Product Images from "Angiotensin II type 2 receptor regulates ROMK-like K+ channel activity in the renal cortical collecting duct during high dietary K+ adaptation"

    Article Title: Angiotensin II type 2 receptor regulates ROMK-like K+ channel activity in the renal cortical collecting duct during high dietary K+ adaptation

    Journal: American Journal of Physiology - Renal Physiology

    doi: 10.1152/ajprenal.00141.2014

    The stimulatory effect of ANG II on ROMK channel activity in CCDs of HK-fed rats is mediated through a nitric oxide (NO)/cGMP pathway. In CCDs from HK-fed rats,  N -nitro- l -arginine methyl ester ( l -NAME; 200 nM) and 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide
    Figure Legend Snippet: The stimulatory effect of ANG II on ROMK channel activity in CCDs of HK-fed rats is mediated through a nitric oxide (NO)/cGMP pathway. In CCDs from HK-fed rats, N -nitro- l -arginine methyl ester ( l -NAME; 200 nM) and 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide

    Techniques Used: Activity Assay

    15) Product Images from "Peptide-Stimulated Angiogenesis: Role of Lung Endothelial Caveolar Signaling and Nitric Oxide"

    Article Title: Peptide-Stimulated Angiogenesis: Role of Lung Endothelial Caveolar Signaling and Nitric Oxide

    Journal: Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society

    doi: 10.1016/j.niox.2015.10.002

    P1 failed to enhance eNOS activity and angiogenesis in the presence of eNOS inhibitor L-NAME or PKC-α inhibitor GO6979 (GO). ECs were pretreated overnight with L-NAME (1 mM) or GO (10 μM) in RPMI 1640 followed by 60 min at 37°C
    Figure Legend Snippet: P1 failed to enhance eNOS activity and angiogenesis in the presence of eNOS inhibitor L-NAME or PKC-α inhibitor GO6979 (GO). ECs were pretreated overnight with L-NAME (1 mM) or GO (10 μM) in RPMI 1640 followed by 60 min at 37°C

    Techniques Used: Activity Assay

    16) Product Images from "Reactive Oxygen Species Induce Antiviral Innate Immune Response through IFN-λ Regulation in Human Nasal Epithelial Cells"

    Article Title: Reactive Oxygen Species Induce Antiviral Innate Immune Response through IFN-λ Regulation in Human Nasal Epithelial Cells

    Journal: American Journal of Respiratory Cell and Molecular Biology

    doi: 10.1165/rcmb.2013-0003OC

    Mitochondria and Duox2 are involved in intracellular ROS generation after IAV infection in the nasal epithelium. After pretreating NHNE cells with 10 μM N G -monoethyl- l -arginine (NMEA), 100 μM allopurinol, and 500 μM mitoTEMPO (all
    Figure Legend Snippet: Mitochondria and Duox2 are involved in intracellular ROS generation after IAV infection in the nasal epithelium. After pretreating NHNE cells with 10 μM N G -monoethyl- l -arginine (NMEA), 100 μM allopurinol, and 500 μM mitoTEMPO (all

    Techniques Used: Infection

    17) Product Images from "Exercise Training Prevents Arterial Baroreflex Dysfunction in Rats Treated With Central Angiotensin II"

    Article Title: Exercise Training Prevents Arterial Baroreflex Dysfunction in Rats Treated With Central Angiotensin II

    Journal: Hypertension

    doi: 10.1161/01.HYP.0000256955.74461.93

    Hypothalamic superoxide production in the basal state (A) or in the presence of NAD(P)H (10 μmol/L; B). Apocynin (1 mmol/L) and N G -monomethyl-L-arginine N G -monomethyl-L-arginine (1 mmol/L) were used to inhibit the activity of NAD(P)H oxidase and NOS, respectively. * P
    Figure Legend Snippet: Hypothalamic superoxide production in the basal state (A) or in the presence of NAD(P)H (10 μmol/L; B). Apocynin (1 mmol/L) and N G -monomethyl-L-arginine N G -monomethyl-L-arginine (1 mmol/L) were used to inhibit the activity of NAD(P)H oxidase and NOS, respectively. * P

    Techniques Used: Activity Assay

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    Animal Model:

    Article Title: Interleukin-22 ameliorates acute severe pancreatitis-associated lung injury in mice
    Article Snippet: Paragraph title: Animal model of SAP and treatments ... Mice in the SAP, PBS and rIL-22 groups were injected intraperitoneally (ip) twice with 20% L-arginine hydrochloride (Sigma-Aldrich; pH = 7.0, 4 g/kg bodyweight), at an interval of 1 h. The normal control group received physiological saline injections.

    IV Injection:

    Article Title: Fluid resuscitation therapy in endotoxemic hamsters improves survival and attenuates capillary perfusion deficits and inflammatory responses by a mechanism related to nitric oxide
    Article Snippet: Immediately after baseline determination of hemodynamic and microcirculatory parameters and evaluation of leukocyte-endothelial interactions, endotoxemia was induced by an IV injection of 1 mg.kg−1 of Escherichia coli serotype 055:B5 lipopolysaccharide (LPS; Sigma-Aldrich, St. Louis, MO, USA) diluted in normal saline (total volume of 0.2 ml). .. After endotoxemia induction, animals were randomly allocated in one of three study groups: (1) LPS (n = 10) – no further treatment after LPS injection; (2) LPS/FR (n = 10) – one hour after LPS injection animals were fluid resuscitated with normal saline (40 ml.kg−1 in 30 minutes); (3) LPS/FR/LNNA (n = 10) – one hour after LPS injection animals were treated with L-Nω -Nitroarginine (L-NNA; 0.5 mg.kg−1 IV; 0.2 ml; Sigma-Aldrich, St. Louis, MO, USA) and then fluid resuscitated with normal saline (40 ml.kg−1 in 30 minutes), and one hour after fluid resuscitation, animals were treated with L-Arginine hydrochloride (100 mg.kg−1 IV; 0.2 ml; Sigma-Aldrich, St. Louis, MO, USA).

    Sampling:

    Article Title: Fluid resuscitation therapy in endotoxemic hamsters improves survival and attenuates capillary perfusion deficits and inflammatory responses by a mechanism related to nitric oxide
    Article Snippet: After endotoxemia induction, animals were randomly allocated in one of three study groups: (1) LPS (n = 10) – no further treatment after LPS injection; (2) LPS/FR (n = 10) – one hour after LPS injection animals were fluid resuscitated with normal saline (40 ml.kg−1 in 30 minutes); (3) LPS/FR/LNNA (n = 10) – one hour after LPS injection animals were treated with L-Nω -Nitroarginine (L-NNA; 0.5 mg.kg−1 IV; 0.2 ml; Sigma-Aldrich, St. Louis, MO, USA) and then fluid resuscitated with normal saline (40 ml.kg−1 in 30 minutes), and one hour after fluid resuscitation, animals were treated with L-Arginine hydrochloride (100 mg.kg−1 IV; 0.2 ml; Sigma-Aldrich, St. Louis, MO, USA). .. Blood sampling for biochemical and hematological analysis was performed in all groups at the end of the study period (t = 3 h).

    Negative Control:

    Article Title: A Thermolabile Aldolase A Mutant Causes Fever-Induced Recurrent Rhabdomyolysis without Hemolytic Anemia
    Article Snippet: The non-targeting siRNA #2 (Dharmacon) was used as a negative control. .. The effect of arginine supplementation was investigated in patients' myoblasts incubated with 2 mM of L-arginine hydrochloride (Sigma) for 10 days.

    Knock-Out:

    Article Title: Enhanced Neutrophil Extracellular Trap Formation in Acute Pancreatitis Contributes to Disease Severity and Is Reduced by Chloroquine
    Article Snippet: PAD4 knockout (PAD4−/− ) mice, incapable of forming NETs, were obtained as a kind gift from the late Dr. Kerri Mowen ( ) and were generated on a C57/Bl6 background. .. Briefly, a sterile solution of 8% L-arginine hydrochloride (A92600, Millipore Sigma, Burlington, MA) was prepared in normal saline and adjusted to pH 7.0.

    Mouse Assay:

    Article Title: The fusion of autophagosome with lysosome is impaired in L-arginine-induced acute pancreatitis
    Article Snippet: The 10 male KUNMING mice were equally and randomly assigned to two groups: normal saline (NS) group and the experimental group. .. For L-arginine-induced pancreatitis, a sterile solution of L-arginine hydrochloride (20%; Sigma) was prepared in normal saline and the pH was adjusted to 7.0.

    Article Title: Intracellular Hmgb1 Inhibits Inflammatory Nucleosome Release and Limits Acute Pancreatitis in Mice
    Article Snippet: For L-arginine-induced pancreatitis, a sterile solution of L-arginine hydrochloride (8%; Sigma) was prepared in normal saline and the pH was adjusted to 7.0. .. Mice received two hourly intraperitoneal (i.p.) injections of L-arginine (4 g/kg), while controls were administered saline i.p. as a control as described previously .

    Article Title: Interleukin-22 ameliorates acute severe pancreatitis-associated lung injury in mice
    Article Snippet: .. Mice in the SAP, PBS and rIL-22 groups were injected intraperitoneally (ip) twice with 20% L-arginine hydrochloride (Sigma-Aldrich; pH = 7.0, 4 g/kg bodyweight), at an interval of 1 h. The normal control group received physiological saline injections. .. PBS or rIL-22 (Miltenyi Biotech) (200 ng/per, 5 times) was administered subcutaneously to mice in the PBS and rIL-22 groups (Figure ).

    Article Title: The Receptor for Advanced Glycation Endproducts (RAGE) Activates the AIM2 Inflammasome in Acute Pancreatitis
    Article Snippet: TLR4−/− mice (C57BL/6 background) and AIM2−/− mice (C57BL/6 background) were obtained from The Jackson Laboratory (Farmington, CT, USA). .. For L-arginine-induced pancreatitis, a sterile solution of L-arginine hydrochloride (8%; Sigma) was prepared in normal saline and the pH was adjusted to 7.0.

    Article Title: Enhanced Neutrophil Extracellular Trap Formation in Acute Pancreatitis Contributes to Disease Severity and Is Reduced by Chloroquine
    Article Snippet: Induction of AP using L-arginine ( ) or choline deficient ethionine (CDE) supplemented diet ( ) was performed as previously described in age and gender matched mice ( ). .. Briefly, a sterile solution of 8% L-arginine hydrochloride (A92600, Millipore Sigma, Burlington, MA) was prepared in normal saline and adjusted to pH 7.0.

    Labeling:

    Article Title: Using the Ubiquitin-modified Proteome to Monitor Distinct and Spatially Restricted Protein Homeostasis Dysfunction *
    Article Snippet: .. For stable isotope labeling by amino acids in cell culture (SILAC) experiments, cells were cultured in custom DMEM without arginine or lysine (Mediatech) supplemented with 10% dialyzed FBS (Life Technologies), penicillin (50 I.U./ml) streptomycin (50 μg/ml) (Mediatech), l -Arginine hydrochloride (85 μg/ml Sigma, St. Louis, MO) and either “light” l -Lysine hydrochloride (50 μg/ml Sigma) or heavy 13C6,15N2 l -Lysine-hydrochloride (50 μg/ml Cambridge Isotopes, Tewesbury, MA) and 292 μg/ml l -Glutamine (Mediatech). .. All cell lines were grown at 37 °C in the presence of 5% CO2 .

    Article Title: Active Protein Neddylation or Ubiquitylation Is Dispensable for Stress Granule Dynamics
    Article Snippet: .. SILAC labeling For stable isotope labeling by amino acids in cell culture (SILAC) experiments, cells were cultured in custom DMEM without arginine or lysine (Mediatech) supplemented with 10% dialyzed FBS (Life Technologies), 1% penicillin/streptomycin, L-Arginine hydrochloride (85mg/ml Sigma) and either “light” L-Lysine hydrochloride (50μg/ml Sigma) or heavy 13C6,15N2 L-Lysine-hydrochloride (50mg/ml Cambridge Isotopes) and 292 mg/mL L-Glutamine (Mediatech). .. Mass Spectrometry Heavy and light labeled cells were mixed 1:1 and processed for proteomics and diGLY-immuno-affinity enrichment as described previously( ; ).

    Article Title: A Thermolabile Aldolase A Mutant Causes Fever-Induced Recurrent Rhabdomyolysis without Hemolytic Anemia
    Article Snippet: The effect of arginine supplementation was investigated in patients' myoblasts incubated with 2 mM of L-arginine hydrochloride (Sigma) for 10 days. .. Uniformly stable isotope labeled (U-13 C5 ) glutamine or (U-13 C6 ) arginine (Eurisotop, Saint-Aubin, France) were provided to myoblasts (1 mM) in the presence of glucose (2.5 mM) and incubated without serum for 6 hours.

    Concentration Assay:

    Article Title: Experimental Models in Syrian Golden Hamster Replicate Human Acute Pancreatitis
    Article Snippet: .. Sterile solution of L-arginine hydrochloride (Sigma Aldrich Chemie GmbH, Steinheim, Germany) was prepared in normal saline at the concentration of 8% and pH 7. .. Intraperitoneal injection of the sterile arginine solution at a dose of 1.5-6 g/kg twice hourly served as acute pancreatitis group, and the pancreatitis-free controls were injected with saline alone.

    Incubation:

    Article Title: A Thermolabile Aldolase A Mutant Causes Fever-Induced Recurrent Rhabdomyolysis without Hemolytic Anemia
    Article Snippet: .. The effect of arginine supplementation was investigated in patients' myoblasts incubated with 2 mM of L-arginine hydrochloride (Sigma) for 10 days. .. Three other chaperons (sigma) were also tested, glycerol (100 mM) for 10 days, betaine (10 and 50 mM) , and benzylhydantoin (130 µM) for 3 days.

    other:

    Article Title: Growth parameters of Liberibacter crescens suggest ammonium and phosphate as essential molecules in the Liberibacter-plant host interface
    Article Snippet: Citric acid was purchased through Fisher Scientific (Pittsburgh, PA), L-arginine hydrochloride through Calbiochem (San Diego, CA), L-lysine monohydrochloride through Acros-organics (Geel, Belgium), and methionine sulfoxide through Alfa Aesar (Haverhill, MA).

    Article Title: Cissus sicyoides: Pharmacological Mechanisms Involved in the Anti-Inflammatory and Antidiarrheal Activities
    Article Snippet: Drugs and Chemicals The following substances were used: xylene, arachidonic acid, loperamide, castor oil, atropine, naloxone, yohimbine, clonidine, morphine, carbachol, N ω-nitro-l -arginine, dexamethasone, l -arginine hydrochloride were purchased from Sigma-Aldrich Chemicals Company (St. Louis, MO, USA).

    Article Title: Arginase attenuates inhibitory nonadrenergic noncholinergic nerve-induced nitric oxide generation and airway smooth muscle relaxation
    Article Snippet: Chemicals Histamine dihydrochloride, indomethacin, atropine sulphate, Nω -nitro-L-arginine, (-)-isoprenaline hydrochloride and L-arginine hydrochloride were obtained from Sigma Chemical Co. (St. Louis, MO, USA).

    Article Title: Argininosuccinate Synthase 1-Deficiency Enhances the Cell Sensitivity to Arginine through Decreased DEPTOR Expression in Endometrial Cancer
    Article Snippet: Reagents Reagents were obtained from the following sources: Puromycin was from Merck Millipore (Bedford, MA); Torin1 was from Cell Signaling Technology; Cycloleucine and GSK343 were from Sigma-Aldrich; L-arginine hydrochloride, L-lysine hydrochloride, and L-glutamine were from Wako (Osaka, Japan); Dialyzed Fetal Bovine Serum (FBS) and glutamine-, lysine-, and arginine-free DMEM were from Thermo Fisher Scientific (Waltham, MA).

    Cell Culture:

    Article Title: Using the Ubiquitin-modified Proteome to Monitor Distinct and Spatially Restricted Protein Homeostasis Dysfunction *
    Article Snippet: .. For stable isotope labeling by amino acids in cell culture (SILAC) experiments, cells were cultured in custom DMEM without arginine or lysine (Mediatech) supplemented with 10% dialyzed FBS (Life Technologies), penicillin (50 I.U./ml) streptomycin (50 μg/ml) (Mediatech), l -Arginine hydrochloride (85 μg/ml Sigma, St. Louis, MO) and either “light” l -Lysine hydrochloride (50 μg/ml Sigma) or heavy 13C6,15N2 l -Lysine-hydrochloride (50 μg/ml Cambridge Isotopes, Tewesbury, MA) and 292 μg/ml l -Glutamine (Mediatech). .. All cell lines were grown at 37 °C in the presence of 5% CO2 .

    Article Title: Active Protein Neddylation or Ubiquitylation Is Dispensable for Stress Granule Dynamics
    Article Snippet: .. SILAC labeling For stable isotope labeling by amino acids in cell culture (SILAC) experiments, cells were cultured in custom DMEM without arginine or lysine (Mediatech) supplemented with 10% dialyzed FBS (Life Technologies), 1% penicillin/streptomycin, L-Arginine hydrochloride (85mg/ml Sigma) and either “light” L-Lysine hydrochloride (50μg/ml Sigma) or heavy 13C6,15N2 L-Lysine-hydrochloride (50mg/ml Cambridge Isotopes) and 292 mg/mL L-Glutamine (Mediatech). .. Mass Spectrometry Heavy and light labeled cells were mixed 1:1 and processed for proteomics and diGLY-immuno-affinity enrichment as described previously( ; ).

    Mass Spectrometry:

    Article Title: A Thermolabile Aldolase A Mutant Causes Fever-Induced Recurrent Rhabdomyolysis without Hemolytic Anemia
    Article Snippet: The effect of arginine supplementation was investigated in patients' myoblasts incubated with 2 mM of L-arginine hydrochloride (Sigma) for 10 days. .. Krebs cycle intermediates and their isotopomers were measured by gas chromatography mass spectrometry (Scion TQ, Brüker) using standard derivation by BSTFA [N,O-Bis(trimethylsilyl)trifluoroacetamide] and 1% trimethylchlorosilane.

    Staining:

    Article Title: The fusion of autophagosome with lysosome is impaired in L-arginine-induced acute pancreatitis
    Article Snippet: For L-arginine-induced pancreatitis, a sterile solution of L-arginine hydrochloride (20%; Sigma) was prepared in normal saline and the pH was adjusted to 7.0. .. Formalin-fixed pancreas samples were processed, and 5 μm thick paraffin sections were stained with hematoxylin and eosin (H & E) for histological analysis.

    Western Blot:

    Article Title: The fusion of autophagosome with lysosome is impaired in L-arginine-induced acute pancreatitis
    Article Snippet: For L-arginine-induced pancreatitis, a sterile solution of L-arginine hydrochloride (20%; Sigma) was prepared in normal saline and the pH was adjusted to 7.0. .. All 10 mice were killed 24 h after intraperitoneal injections, pancreatic tissue samples were collected, snap frozen in liquid nitrogen, and stored at -80°C for western blotting.

    Injection:

    Article Title: Fluid resuscitation therapy in endotoxemic hamsters improves survival and attenuates capillary perfusion deficits and inflammatory responses by a mechanism related to nitric oxide
    Article Snippet: .. After endotoxemia induction, animals were randomly allocated in one of three study groups: (1) LPS (n = 10) – no further treatment after LPS injection; (2) LPS/FR (n = 10) – one hour after LPS injection animals were fluid resuscitated with normal saline (40 ml.kg−1 in 30 minutes); (3) LPS/FR/LNNA (n = 10) – one hour after LPS injection animals were treated with L-Nω -Nitroarginine (L-NNA; 0.5 mg.kg−1 IV; 0.2 ml; Sigma-Aldrich, St. Louis, MO, USA) and then fluid resuscitated with normal saline (40 ml.kg−1 in 30 minutes), and one hour after fluid resuscitation, animals were treated with L-Arginine hydrochloride (100 mg.kg−1 IV; 0.2 ml; Sigma-Aldrich, St. Louis, MO, USA). .. As shown in Figure , sequential measurements of hemodynamic and microcirculatory parameters and evaluations of leukocyte-endothelial interactions were performed in four time points: at baseline (Baseline) and after one (t = 1 h), two (t = 2 h), and three (t = 3 h) hours of LPS injection.

    Article Title: Interleukin-22 ameliorates acute severe pancreatitis-associated lung injury in mice
    Article Snippet: .. Mice in the SAP, PBS and rIL-22 groups were injected intraperitoneally (ip) twice with 20% L-arginine hydrochloride (Sigma-Aldrich; pH = 7.0, 4 g/kg bodyweight), at an interval of 1 h. The normal control group received physiological saline injections. .. PBS or rIL-22 (Miltenyi Biotech) (200 ng/per, 5 times) was administered subcutaneously to mice in the PBS and rIL-22 groups (Figure ).

    Article Title: Experimental Models in Syrian Golden Hamster Replicate Human Acute Pancreatitis
    Article Snippet: The hamsters injected with saline alone served as pancreatitis-free controls. .. Sterile solution of L-arginine hydrochloride (Sigma Aldrich Chemie GmbH, Steinheim, Germany) was prepared in normal saline at the concentration of 8% and pH 7.

    Article Title: Enhanced Neutrophil Extracellular Trap Formation in Acute Pancreatitis Contributes to Disease Severity and Is Reduced by Chloroquine
    Article Snippet: Briefly, a sterile solution of 8% L-arginine hydrochloride (A92600, Millipore Sigma, Burlington, MA) was prepared in normal saline and adjusted to pH 7.0. .. Animals were treated with oral chloroquine (CQ) (0.5 mg/ml) administered in the drinking water ad libitum upon completion of second L-arginine injection.

    Recombinant:

    Article Title: Interleukin-22 ameliorates acute severe pancreatitis-associated lung injury in mice
    Article Snippet: Mice in the SAP, PBS and rIL-22 groups were injected intraperitoneally (ip) twice with 20% L-arginine hydrochloride (Sigma-Aldrich; pH = 7.0, 4 g/kg bodyweight), at an interval of 1 h. The normal control group received physiological saline injections. .. Mice in the SAP, PBS and rIL-22 groups were injected intraperitoneally (ip) twice with 20% L-arginine hydrochloride (Sigma-Aldrich; pH = 7.0, 4 g/kg bodyweight), at an interval of 1 h. The normal control group received physiological saline injections.

    Gas Chromatography:

    Article Title: A Thermolabile Aldolase A Mutant Causes Fever-Induced Recurrent Rhabdomyolysis without Hemolytic Anemia
    Article Snippet: The effect of arginine supplementation was investigated in patients' myoblasts incubated with 2 mM of L-arginine hydrochloride (Sigma) for 10 days. .. Krebs cycle intermediates and their isotopomers were measured by gas chromatography mass spectrometry (Scion TQ, Brüker) using standard derivation by BSTFA [N,O-Bis(trimethylsilyl)trifluoroacetamide] and 1% trimethylchlorosilane.

    Generated:

    Article Title: A Thermolabile Aldolase A Mutant Causes Fever-Induced Recurrent Rhabdomyolysis without Hemolytic Anemia
    Article Snippet: Fatty-acid oxidation (FAO) measurements were performed through the assay of deuterated C2 to C16 acylcarnitines generated by incubation of intact myoblasts with a pentadeuterated C16 fatty acid ([16-2 H3 , 15-2 H2 ]-palmitate) according to a procedure used for the detection of β-oxidation defects. .. The effect of arginine supplementation was investigated in patients' myoblasts incubated with 2 mM of L-arginine hydrochloride (Sigma) for 10 days.

    Article Title: Enhanced Neutrophil Extracellular Trap Formation in Acute Pancreatitis Contributes to Disease Severity and Is Reduced by Chloroquine
    Article Snippet: PAD4 knockout (PAD4−/− ) mice, incapable of forming NETs, were obtained as a kind gift from the late Dr. Kerri Mowen ( ) and were generated on a C57/Bl6 background. .. Briefly, a sterile solution of 8% L-arginine hydrochloride (A92600, Millipore Sigma, Burlington, MA) was prepared in normal saline and adjusted to pH 7.0.

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    Millipore glycine arginine glycine aspartic acid serine proline rgd peptide
    Glycine Arginine Glycine Aspartic Acid Serine Proline Rgd Peptide, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Millipore anti srsf2 antibody
    A 10 nt RNA sequence surrounding the BP of intron7 is required for splicing inhibition by <t>SRSF2.</t> (A) (Left panel) Schematic diagram of the M1/E8, A1–E7, and E7/M2 minigenes. (Right panel) RT-PCR analysis of intron splicing in the minigenes in SRSF2-expressing cells. (B) (Left panel) Schematic diagram of M-I (50) and M-E8 minigenes. (Right panel) RT-PCR analysis of the minigenes in SRSF2-expressing cells. (C) (Left panel) Schematic diagram of M-I (50′, 40′, 30′, 20′, 10′ and 5′) minigenes. Green lines represent intron sequences from AdML and black lines represent introns from SMN. The exon and intron lengths are also shown. (Right panel) RT-PCR analysis of intron splicing of the minigenes in the SRSF2-expressing cells. (D) The 10 nt regulatory sequence and its location within the AdML minigene are indicated.
    Anti Srsf2 Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Millipore l arginine hydrochloride
    A 10 nt RNA sequence surrounding the BP of intron7 is required for splicing inhibition by <t>SRSF2.</t> (A) (Left panel) Schematic diagram of the M1/E8, A1–E7, and E7/M2 minigenes. (Right panel) RT-PCR analysis of intron splicing in the minigenes in SRSF2-expressing cells. (B) (Left panel) Schematic diagram of M-I (50) and M-E8 minigenes. (Right panel) RT-PCR analysis of the minigenes in SRSF2-expressing cells. (C) (Left panel) Schematic diagram of M-I (50′, 40′, 30′, 20′, 10′ and 5′) minigenes. Green lines represent intron sequences from AdML and black lines represent introns from SMN. The exon and intron lengths are also shown. (Right panel) RT-PCR analysis of intron splicing of the minigenes in the SRSF2-expressing cells. (D) The 10 nt regulatory sequence and its location within the AdML minigene are indicated.
    L Arginine Hydrochloride, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    A 10 nt RNA sequence surrounding the BP of intron7 is required for splicing inhibition by SRSF2. (A) (Left panel) Schematic diagram of the M1/E8, A1–E7, and E7/M2 minigenes. (Right panel) RT-PCR analysis of intron splicing in the minigenes in SRSF2-expressing cells. (B) (Left panel) Schematic diagram of M-I (50) and M-E8 minigenes. (Right panel) RT-PCR analysis of the minigenes in SRSF2-expressing cells. (C) (Left panel) Schematic diagram of M-I (50′, 40′, 30′, 20′, 10′ and 5′) minigenes. Green lines represent intron sequences from AdML and black lines represent introns from SMN. The exon and intron lengths are also shown. (Right panel) RT-PCR analysis of intron splicing of the minigenes in the SRSF2-expressing cells. (D) The 10 nt regulatory sequence and its location within the AdML minigene are indicated.

    Journal: BMB Reports

    Article Title: SRSF2 directly inhibits intron splicing to suppresses cassette exon inclusion

    doi: 10.5483/BMBRep.2017.50.8.103

    Figure Lengend Snippet: A 10 nt RNA sequence surrounding the BP of intron7 is required for splicing inhibition by SRSF2. (A) (Left panel) Schematic diagram of the M1/E8, A1–E7, and E7/M2 minigenes. (Right panel) RT-PCR analysis of intron splicing in the minigenes in SRSF2-expressing cells. (B) (Left panel) Schematic diagram of M-I (50) and M-E8 minigenes. (Right panel) RT-PCR analysis of the minigenes in SRSF2-expressing cells. (C) (Left panel) Schematic diagram of M-I (50′, 40′, 30′, 20′, 10′ and 5′) minigenes. Green lines represent intron sequences from AdML and black lines represent introns from SMN. The exon and intron lengths are also shown. (Right panel) RT-PCR analysis of intron splicing of the minigenes in the SRSF2-expressing cells. (D) The 10 nt regulatory sequence and its location within the AdML minigene are indicated.

    Article Snippet: The membrane was probed with an anti-SRSF2 antibody (Millipore) and the antibody-SRSF2 conjugates were detected by enhanced chemiluminescence (ECL).

    Techniques: Sequencing, Inhibition, Reverse Transcription Polymerase Chain Reaction, Expressing

    A 10 nt RNA sequence physically interacts with SRSF2 and inhibits constitutive splicing. (A) (Upper panel) The 10 nt regulatory sequence with surrounding sequences and biotin-labeled RNA sequence (10B) are shown. (Lower panel) RNA-pulldown and immunoprecipitation analyses with SRSF2-specific antibody are shown. (B) (Upper panel) Schematic diagram of the AdML-M minigene. (Lower panel) RT-PCR analysis of intron splicing in the AdML-M minigene.

    Journal: BMB Reports

    Article Title: SRSF2 directly inhibits intron splicing to suppresses cassette exon inclusion

    doi: 10.5483/BMBRep.2017.50.8.103

    Figure Lengend Snippet: A 10 nt RNA sequence physically interacts with SRSF2 and inhibits constitutive splicing. (A) (Upper panel) The 10 nt regulatory sequence with surrounding sequences and biotin-labeled RNA sequence (10B) are shown. (Lower panel) RNA-pulldown and immunoprecipitation analyses with SRSF2-specific antibody are shown. (B) (Upper panel) Schematic diagram of the AdML-M minigene. (Lower panel) RT-PCR analysis of intron splicing in the AdML-M minigene.

    Article Snippet: The membrane was probed with an anti-SRSF2 antibody (Millipore) and the antibody-SRSF2 conjugates were detected by enhanced chemiluminescence (ECL).

    Techniques: Sequencing, Labeling, Immunoprecipitation, Reverse Transcription Polymerase Chain Reaction

    SRSF2 promotes exon exclusion. (A) Schematic diagram of the E6–8 minigene. Exons are depicted as numbered boxes, introns as solid lines. (B) RT-PCR analysis of the E6–8 minigene from the SMN1/2 locus in SRSF2-expressing cells. (C) RT-PCR analysis of intron6 splicing within the E6–8 minigene using primers #1 and #2. (D) RT-PCR analysis of intron7 splicing within the E6–8 minigene using primers #3 and #4. (E) RT-PCR analysis to detect alternative splicing of endogenous SMN1 and SMN2 using RNA extracted from cells infected with lentiviruses with SRSF2-targeting shRNA (SRSF2) or non-silencing shRNAs (NS).

    Journal: BMB Reports

    Article Title: SRSF2 directly inhibits intron splicing to suppresses cassette exon inclusion

    doi: 10.5483/BMBRep.2017.50.8.103

    Figure Lengend Snippet: SRSF2 promotes exon exclusion. (A) Schematic diagram of the E6–8 minigene. Exons are depicted as numbered boxes, introns as solid lines. (B) RT-PCR analysis of the E6–8 minigene from the SMN1/2 locus in SRSF2-expressing cells. (C) RT-PCR analysis of intron6 splicing within the E6–8 minigene using primers #1 and #2. (D) RT-PCR analysis of intron7 splicing within the E6–8 minigene using primers #3 and #4. (E) RT-PCR analysis to detect alternative splicing of endogenous SMN1 and SMN2 using RNA extracted from cells infected with lentiviruses with SRSF2-targeting shRNA (SRSF2) or non-silencing shRNAs (NS).

    Article Snippet: The membrane was probed with an anti-SRSF2 antibody (Millipore) and the antibody-SRSF2 conjugates were detected by enhanced chemiluminescence (ECL).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Infection, shRNA

    SRSF2 promotes SMN intron7 retention. (A) (Upper panel) Schematic diagram of the E7–8 and E7–8 (20) minigenes. The primer binding sites are indicated with arrows. (Lower panel) RT-PCR analysis of the E7–8 and E7–8 (20) minigenes in SRSF2-expressing cells. (B) (Upper panel) Nucleotide sequence of a portion of the AdML pre-mRNA. The two exons are shown as dark yellow and yellow boxes, the upstream intron is indicated in orange, and the downstream intron is indicated in green. (Right panel) RT-PCR analysis of the AdML minigene using RNAs in SRSF2-expressing cells.

    Journal: BMB Reports

    Article Title: SRSF2 directly inhibits intron splicing to suppresses cassette exon inclusion

    doi: 10.5483/BMBRep.2017.50.8.103

    Figure Lengend Snippet: SRSF2 promotes SMN intron7 retention. (A) (Upper panel) Schematic diagram of the E7–8 and E7–8 (20) minigenes. The primer binding sites are indicated with arrows. (Lower panel) RT-PCR analysis of the E7–8 and E7–8 (20) minigenes in SRSF2-expressing cells. (B) (Upper panel) Nucleotide sequence of a portion of the AdML pre-mRNA. The two exons are shown as dark yellow and yellow boxes, the upstream intron is indicated in orange, and the downstream intron is indicated in green. (Right panel) RT-PCR analysis of the AdML minigene using RNAs in SRSF2-expressing cells.

    Article Snippet: The membrane was probed with an anti-SRSF2 antibody (Millipore) and the antibody-SRSF2 conjugates were detected by enhanced chemiluminescence (ECL).

    Techniques: Binding Assay, Reverse Transcription Polymerase Chain Reaction, Expressing, Sequencing