l johnsonii jcm 2012  (ATCC)

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Microbiota-Derived Inosine Suppresses Systemic Autoimmunity via Restriction of B Cell Differentiation and Migration.

doi: 10.1002/advs.202409837

Figure Lengend Snippet: Figure 5. L.johnsonii-associated purine metabolite enrichment alleviated systemic autoimmunity. An untargeted metabolomics of the feces in saline- treated and FMT treated pristane-induced mice in 44 weeks was conducted (n = 10 per group). A) Enriched KEGG pathways in fecal metabolites between saline-treated and FMT-treated mice were shown with the P values and the number of metabolites represented in each pathway. The size of each bubble represents the number of metabolites differentially expressed for each pathway, with a scale on the lower right (number). B) The matchstick map according to the differential metabolites obtained from each group of difference comparison combinations in positive mode was drawn, and the up-down of metabolites and the substances with large difference multiples were indicated. C) Pearson’s correlation analysis between metabolites in purine metabolism and significantly differentiated strains was conducted. D) Plasma inosine level was tested between Saline-treated and FMT-treated groups. E) Schematic diagram of GF mice orally treated saline and L. johnsonii (Lj, 2 OD, 0.3 mL−1/time, 6 times in total). Plasma inosine level was tested between saline-treated and Lj-treated groups. F) Overview of Lj and inosine treatment experimental design (n = 6 per group) in pristane-induced C57 mice. Orally gavage Lj (2 OD, 0.3 mL−1/time, 3 times/week), inosine (50 mg k−1g, 0.3 mL−1/time, 3 times/week), or saline started at 21 weeks of age, and lasted until 36 weeks of age. The amount of inosine in plasma from saline-treated, Lj-treated and inosine-treated pristane-induced mice was detected. G) Plasma anti-dsDNA level was detected among HC, saline-treated, Lj-treated, and inosine-treated groups. H) Statistical analyses were conducted to compare the systemic frequencies of CD4 naïve T cells and CD4 TEM cells, CD8 naïve T cells and CD8 TEM cells among HC, saline-treated, Lj-treated, and inosine-treated groups. I,J) Statistical analyses were conducted to compare the systemic frequencies of I) naïve B and J) memory B cells among HC, saline-treated, Lj-treated, and inosine-treated groups. K) Statistical analyses were conducted to compare the systemic frequencies and cell number of GCB among saline-treated, Lj-treated, and inosine-treated groups. The results are expressed as mean ± SEM. Statistical comparison was based on Student t-test and a one-way ANOVA. *p < 0.05 was considered statistically significant. **p < 0.01; ***p < 0.001.

Article Snippet: L. Johnsonii and Inosine Oral Gavage Experiments: Pristaneinduced C57BL/6 mice and MRL/lpr mice were colonized by oral gavage with 2 × 109 CFU of L. johnsonii, or 50 mg kg−1 inosine (MCE, HY-N0092), 300 μL−1/times, 3 times/week.

Techniques: Saline, Comparison, Clinical Proteomics

Journal: Journal of Nanobiotechnology

Article Title: Lactobacillus johnsonii -derived extracellular vesicles restore mucosal immunity via taurine-linked Th17/Treg and IgA/IgG regulation in colitis

doi: 10.1186/s12951-025-03702-6

Figure Lengend Snippet: L. johnsonii depletion in UC and characterization of its derived EVs. ( A ) 16 S rRNA sequencing of gut microbiota in UC patients ( n = 9) vs. healthy controls (HC, n = 6). ( B ) LEfSe-based LDA analysis showing reduced Lactobacillus abundance in active UC. ( C ) Decreased L. johnsonii abundance in active UC compared to HC. ( D ) TEM analysis of L. johnsonii -derived EVs. ( E ) EVs’ diameter ranges from 100 nm to 200 nm, with a median of 152 nm. ( F ) 3D surface plot of EVs. ( G ) TEM analysis of L. johnsonii -derived EVs in PBS, SGF and SIF (×20000). Data are presented as mean ± SD. * p < 0.05

Article Snippet: The detailed procedures for the isolation and purification of L. johnsonii (ATCC 33200) from healthy donors have been covered in our previous studies [ – ], and its nucleotide sequence information is provided in Supplementary Table 3.

Techniques: Derivative Assay, Sequencing

Journal: Journal of Nanobiotechnology

Article Title: Lactobacillus johnsonii -derived extracellular vesicles restore mucosal immunity via taurine-linked Th17/Treg and IgA/IgG regulation in colitis

doi: 10.1186/s12951-025-03702-6

Figure Lengend Snippet: Tracking biodistribution and epithelial association of L. johnsonii -derived EVs in vivo. ( A ) In vivo imaging showing the dynamic distribution of DiI-labeled L. johnsonii -derived EVs in the heart, brain, Liver, spleen, lung, kidney, stomach, and intestine of DSS-induced colitis mice at 4, 12, 24, and 48 h post-administration. ( B ) Confocal microscopy images 6 h post-gavage showing colonic distribution of DiI-labeled EVs (red) and FITC-d-Lys-labeled L. johnsonii (green) in DSS mice (×500). EVs were closely associated with the colonic epithelium, whereas L. johnsonii primarily localized within the crypt mesenchyme

Article Snippet: The detailed procedures for the isolation and purification of L. johnsonii (ATCC 33200) from healthy donors have been covered in our previous studies [ – ], and its nucleotide sequence information is provided in Supplementary Table 3.

Techniques: Derivative Assay, In Vivo, In Vivo Imaging, Labeling, Confocal Microscopy

Journal: Journal of Nanobiotechnology

Article Title: Lactobacillus johnsonii -derived extracellular vesicles restore mucosal immunity via taurine-linked Th17/Treg and IgA/IgG regulation in colitis

doi: 10.1186/s12951-025-03702-6

Figure Lengend Snippet: Biological safety of L. johnsonii -derived EVs in mice. ( A ) H&E staining showed no pathological changes in the heart, liver, spleen, lung, or kidney. ( B ) Biochemical analysis showed no abnormalities in plasma CK-MB, ALT, AST, creatinine (Crea), or urea levels. ns, not significant

Article Snippet: The detailed procedures for the isolation and purification of L. johnsonii (ATCC 33200) from healthy donors have been covered in our previous studies [ – ], and its nucleotide sequence information is provided in Supplementary Table 3.

Techniques: Derivative Assay, Staining, Clinical Proteomics

Journal: Journal of Nanobiotechnology

Article Title: Lactobacillus johnsonii -derived extracellular vesicles restore mucosal immunity via taurine-linked Th17/Treg and IgA/IgG regulation in colitis

doi: 10.1186/s12951-025-03702-6

Figure Lengend Snippet: Both L. johnsonii and its derived EVs alleviated DSS-induced colitis and enhanced the gut barrier. ( A ) Body weight loss ( n = 5). ( B ) DAI scores. ( C ) Representative images of the colon. ( D ) Colon length ( n = 5). ( E ) H&E staining (×200) and pathological score of rectum. ( F-G ) IHC images and partial enlargement of Claudin 1 (×200), and its mRNA expression. ( H-I ) IHC images and partial enlargement of Muc2 (×200), and mRNA expression of Muc2. ( J ) mRNA expression of inflammatory markers ( n = 3). Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. Abbreviations: DSS, dextran sulfate sodium

Article Snippet: The detailed procedures for the isolation and purification of L. johnsonii (ATCC 33200) from healthy donors have been covered in our previous studies [ – ], and its nucleotide sequence information is provided in Supplementary Table 3.

Techniques: Derivative Assay, Staining, Expressing

Journal: Journal of Nanobiotechnology

Article Title: Lactobacillus johnsonii -derived extracellular vesicles restore mucosal immunity via taurine-linked Th17/Treg and IgA/IgG regulation in colitis

doi: 10.1186/s12951-025-03702-6

Figure Lengend Snippet: Both L. johnsonii and its derived EVs restored the Th17/Treg balance. ( A ) Relative mRNA expression of lineage-defining transcription factors and signature cytokines for Th1 (T-bet, IFN-γ), Th2 (GATA3, IL-4), Th17 (RORγt, IL-17 A), and Treg (FOXP3, IL-10) cells in colonic tissues from DSS-induced colitis mice following treatment with PBS, L. johnsonii , or EVs ( n = 3). ( B ) Flow cytometric analysis of colonic lamina propria CD4⁺ T cell subsets, including Th1 (CD4⁺T-bet⁺), Th2 (CD4⁺GATA3⁺), Th17 (CD4⁺RORγt⁺), and Treg (CD4⁺CD25⁺FOXP3⁺) populations (n = 4). Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: The detailed procedures for the isolation and purification of L. johnsonii (ATCC 33200) from healthy donors have been covered in our previous studies [ – ], and its nucleotide sequence information is provided in Supplementary Table 3.

Techniques: Derivative Assay, Expressing

Journal: Journal of Nanobiotechnology

Article Title: Lactobacillus johnsonii -derived extracellular vesicles restore mucosal immunity via taurine-linked Th17/Treg and IgA/IgG regulation in colitis

doi: 10.1186/s12951-025-03702-6

Figure Lengend Snippet: Both L. johnsonii and its derived EVs reduced the level of highly Ig-coated gut bacteria. ( A ) Bacterial flow cytometry analysis of highly Ig-bound bacteria in colitis mice ( n = 5). ( B-D ) ELISA results of fecal IgA, IgG, and IgM levels in mice ( n = 4–5). ( E-F ) mRNA expression of PIGR and FcRn in mice colon ( n = 3). ( G ) Western blot analysis of PIGR and FcRn in mice colon. Data are expressed as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. Abbreviation: pIgR, polymeric immunoglobulin receptor; FcRn, neonatal Fragment crystallizable receptor

Article Snippet: The detailed procedures for the isolation and purification of L. johnsonii (ATCC 33200) from healthy donors have been covered in our previous studies [ – ], and its nucleotide sequence information is provided in Supplementary Table 3.

Techniques: Derivative Assay, Bacteria, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot

Journal: Journal of Nanobiotechnology

Article Title: Lactobacillus johnsonii -derived extracellular vesicles restore mucosal immunity via taurine-linked Th17/Treg and IgA/IgG regulation in colitis

doi: 10.1186/s12951-025-03702-6

Figure Lengend Snippet: Both L. johnsonii and its derived EVs remodeled gut microbiota and amino acid metabolism. ( A ) UPGMA analysis of microbiota composition at the phylum level ( n = 4). ( B ) Heatmap of relative abundance of the top 15 taxa at the order level ( n = 5). ( C ) Pearson correlation heatmap between 16 S rRNA abundance and 23 amino acids levels. ( D ) KO enrichment analysis of taurine- and glycine-associated KEGG pathways. Abbreviation: UPGMA, unweighted pair group method with arithmetic mean.

Article Snippet: The detailed procedures for the isolation and purification of L. johnsonii (ATCC 33200) from healthy donors have been covered in our previous studies [ – ], and its nucleotide sequence information is provided in Supplementary Table 3.

Techniques: Derivative Assay

Journal: Journal of Nanobiotechnology

Article Title: Lactobacillus johnsonii -derived extracellular vesicles restore mucosal immunity via taurine-linked Th17/Treg and IgA/IgG regulation in colitis

doi: 10.1186/s12951-025-03702-6

Figure Lengend Snippet: Taurine supplementation reproduced the immunomodulatory and barrier-protective effects of L. johnsonii -derived EVs. ( A ) Body weight changes ( n = 5). ( B ) DAI scores ( n = 5). ( C ) Representative images of colons. ( D ) Colon length ( n = 5). ( E ) Representative H&E-stained colon sections (×200) with corresponding histopathological scores ( n = 5). ( F ) Colonic mRNA expression of inflammatory cytokines, tight junction proteins, Th17/Treg-associated markers, and immunoglobulin transporters ( n = 4). Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: The detailed procedures for the isolation and purification of L. johnsonii (ATCC 33200) from healthy donors have been covered in our previous studies [ – ], and its nucleotide sequence information is provided in Supplementary Table 3.

Techniques: Derivative Assay, Staining, Expressing