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Mediatech l glutamine
L Glutamine, supplied by Mediatech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/l glutamine/product/Mediatech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
l glutamine - by Bioz Stars, 2021-03
86/100 stars

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Infection:

Article Title: Airborne influenza virus detection with four aerosol samplers using molecular and infectivity assays: considerations for a new infectious virus aerosol sampler
Article Snippet: .. Influenza virus infectivity assays were performed on Madin-Darby Canine Kidney Cells (MDCK) (CCL-34; ATCC, Manassas, VA, USA) grown in Dulbecco’s Modification of Eagle’s Medium with glucose and l -glutamine (DMEM) (Mediatech, Herndon, VA, USA) with 10% heat-inactivated fetal bovine serum (Hyclone). ..

Modification:

Article Title: Airborne influenza virus detection with four aerosol samplers using molecular and infectivity assays: considerations for a new infectious virus aerosol sampler
Article Snippet: .. Influenza virus infectivity assays were performed on Madin-Darby Canine Kidney Cells (MDCK) (CCL-34; ATCC, Manassas, VA, USA) grown in Dulbecco’s Modification of Eagle’s Medium with glucose and l -glutamine (DMEM) (Mediatech, Herndon, VA, USA) with 10% heat-inactivated fetal bovine serum (Hyclone). ..

Article Title: Comparison of topical tofacitinib and 0.1% hypochlorous acid in a murine atopic dermatitis model
Article Snippet: Fura-2-acetoxymethyl ester (Fura-2 AM), phosphate buffered saline (PBS), collagenase, chloroquine, histamine, and SLIGRL-NH2 were ordered from Thermo Fisher Scientific Inc. (Waltham, MA, USA). .. Fetal bovine serum (FBS), Dulbecco’s modified eagle medium with L-glutamine (DMEM) and RPMI-1640 medium, Ca2+ and Mg2+ -free hank’s balanced salt solution (HBSS), penicillin-streptomycin and fetal bovine serum (FBS) were from Mediatech Inc. (Manassas, VA, USA). .. MEM eagle (EMEM) medium was obtained from Lonza Group Ltd. (Allendale, NJ, USA).

Article Title: Gene expression profiling of bovine bronchial epithelial cells exposed in vitro to bovine herpesvirus 1 and Mannheimia haemolytica
Article Snippet: .. 2.1 Bovine bronchial epithelial cell culture Primary bovine bronchial epithelial cells (BBEs), generously provided by Dr. Allen-Gipson (University of Nebraska Medical Center), were maintained in Dulbecco's Modified Eagle's Medium/F12 (DMEM) (Cellgro; Mediatech, Inc., Herndon, VA) containing 10% Fetal Bovine Serum (FBS) (Atlanta Biologicals, Lawrenceville, GA), 2 mM l -glutamine (l -glu) (Mediatech), 200 ng/l epithelial growth factor (EGF) (Sigma, St. Louis, MO), 1% penicillin and streptomycin (Cellgro) at 37 °C and 5% CO2 . .. 2.2 M. haemolytica M. haemolytica A1 isolate D153 (a plasmid-negative strain isolated from the lung of a steer that died of pneumonia) was a gift from R. Briggs (Ames, IA).

Isolation:

Article Title: Characterization of Ascites-Derived Ovarian Tumor Cells from Spontaneously Occurring Ovarian Tumors of the Chicken: Evidence for E-Cadherin Upregulation
Article Snippet: Four-µm thick ovary tissue slices were prepared and stained with hematoxylin and eosin for examination by a board-certified veterinary pathologist (TKC). .. Cells present in ascites collected from each animal were isolated by centrifugation at 125×g for 5 min at 25°C and plated into gelatinized T-75 flasks (BD Biosciences, Bedford, MA) in growth medium (GM) that consisted of 1∶1 MCDB105:M199 culture medium (Sigma-Aldrich, St. Louis, MO), 10% chicken serum, 5% horse serum, 5% fetal bovine serum (FBS; Sigma-Aldrich), gelatin (Fisher Scientific, Pittsburgh, PA), MEM amino acid, L-alanine-L-glutamine dipeptide (Mediatech, Manassas, VA), glucose (Invitrogen, Carlsbad, CA), and sodium pyruvate (Sigma-Aldrich). .. In addition, penicillin-streptomycin-fungizone antimicrobial solution (Invitrogen) was supplemented to the GM when growing ascites-derived chicken ovarian cancer cells (COVCAR) during the initial first passage.

Centrifugation:

Article Title: Characterization of Ascites-Derived Ovarian Tumor Cells from Spontaneously Occurring Ovarian Tumors of the Chicken: Evidence for E-Cadherin Upregulation
Article Snippet: Four-µm thick ovary tissue slices were prepared and stained with hematoxylin and eosin for examination by a board-certified veterinary pathologist (TKC). .. Cells present in ascites collected from each animal were isolated by centrifugation at 125×g for 5 min at 25°C and plated into gelatinized T-75 flasks (BD Biosciences, Bedford, MA) in growth medium (GM) that consisted of 1∶1 MCDB105:M199 culture medium (Sigma-Aldrich, St. Louis, MO), 10% chicken serum, 5% horse serum, 5% fetal bovine serum (FBS; Sigma-Aldrich), gelatin (Fisher Scientific, Pittsburgh, PA), MEM amino acid, L-alanine-L-glutamine dipeptide (Mediatech, Manassas, VA), glucose (Invitrogen, Carlsbad, CA), and sodium pyruvate (Sigma-Aldrich). .. In addition, penicillin-streptomycin-fungizone antimicrobial solution (Invitrogen) was supplemented to the GM when growing ascites-derived chicken ovarian cancer cells (COVCAR) during the initial first passage.

Functional Assay:

Article Title: Daedalus: a robust, turnkey platform for rapid production of decigram quantities of active recombinant proteins in human cell lines using novel lentiviral vectors
Article Snippet: .. Functional assays Bone marrow was harvested from C57BL/6 mice (Jackson laboratories) and plated in a six-well dish at 1 × 106 cells/ml in 2 ml of lymphocyte media (RPMI 1640 (Hyclone) supplemented with 10% FCS (Omega Scientific), 5 mM l -Alanyl-l -Glutamine (Mediatech), 100 IU/ml penicillin–streptomycin (Mediatech) and 2 mM β-mercaptoethanol (Gibco)) with 5 ng/ml of Daedalus IL-7 or commercial IL-7 (PeproTech). ..

Mouse Assay:

Article Title: Daedalus: a robust, turnkey platform for rapid production of decigram quantities of active recombinant proteins in human cell lines using novel lentiviral vectors
Article Snippet: .. Functional assays Bone marrow was harvested from C57BL/6 mice (Jackson laboratories) and plated in a six-well dish at 1 × 106 cells/ml in 2 ml of lymphocyte media (RPMI 1640 (Hyclone) supplemented with 10% FCS (Omega Scientific), 5 mM l -Alanyl-l -Glutamine (Mediatech), 100 IU/ml penicillin–streptomycin (Mediatech) and 2 mM β-mercaptoethanol (Gibco)) with 5 ng/ml of Daedalus IL-7 or commercial IL-7 (PeproTech). ..

Cell Culture:

Article Title: Gene expression profiling of bovine bronchial epithelial cells exposed in vitro to bovine herpesvirus 1 and Mannheimia haemolytica
Article Snippet: .. 2.1 Bovine bronchial epithelial cell culture Primary bovine bronchial epithelial cells (BBEs), generously provided by Dr. Allen-Gipson (University of Nebraska Medical Center), were maintained in Dulbecco's Modified Eagle's Medium/F12 (DMEM) (Cellgro; Mediatech, Inc., Herndon, VA) containing 10% Fetal Bovine Serum (FBS) (Atlanta Biologicals, Lawrenceville, GA), 2 mM l -glutamine (l -glu) (Mediatech), 200 ng/l epithelial growth factor (EGF) (Sigma, St. Louis, MO), 1% penicillin and streptomycin (Cellgro) at 37 °C and 5% CO2 . .. 2.2 M. haemolytica M. haemolytica A1 isolate D153 (a plasmid-negative strain isolated from the lung of a steer that died of pneumonia) was a gift from R. Briggs (Ames, IA).

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    Mediatech l glutamine
    L Glutamine, supplied by Mediatech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l glutamine/product/Mediatech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    l glutamine - by Bioz Stars, 2021-03
    86/100 stars
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