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Cellgro l glutamine
L Glutamine, supplied by Cellgro, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/l glutamine/product/Cellgro
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
l glutamine - by Bioz Stars, 2021-03
86/100 stars

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Cell Culture:

Article Title: GATA/Heme Multi-omics Reveals a Trace Metal-Dependent Cellular Differentiation Mechanism
Article Snippet: HEK293T cells derived from a female fetus were cultured in DMEM (Corning, 10013CV) containing 10% FBS and 1% penicillin-streptomycin (Gemini). .. Primary erythroid precursors from E14.5 murine fetal livers were cultured in StemPro-34 (Gibco) supplemented with 10% nutrient supplement (Gibco), 1× L-glutamine (Cellgro), 1% penicillin-streptomycin (Gemini), 0.1 mM monothioglycerol (Sigma), 1 βM dexamethasone (Sigma), 0.5 U/ml erythropoietin (Amgen), and 1% conditioned medium from a Kit ligand-producing CHO cell line (expansion culture). .. After two days, cells were differentiated in IMDM (Gibco) □containing 10% FBS (Gemini), 10% plasma-derived serum (PDS; Animal Technologies), 5% protein-free hybridoma medium II (PFHM II; Gibco), 1% penicillin-streptomycin (Gemini), 0.1 mM monothioglycerol (Sigma) and 6 U/ml of erythropoietin (Amgen).

Article Title: N-BLR, a primate-specific non-coding transcript leads to colorectal cancer invasion and migration
Article Snippet: As control, we used a pool of non-targeting siRNAs (Dharmacon). .. Cell count and viability Colo320, SW620, and SW480 cells were cultured in RPMI 1640 1X with L-Glutamine medium (#10-040-CV, Corning Cellgro), whereas HCT116, RKO, and HT-29 cells were cultured in McCoy’s 5A 1X with L-Glutamine medium (#10-050-CV, Corning Cellgro), supplemented with 10% fetal bovine serum and 1% penicillin and streptomycin. .. CRC cell lines were plated in 24-well plates at a concentration of 1 × 105 cells/well in an antibiotic-free medium one day before transfection.

Modification:

Article Title: Hypoxia Activates Constitutive Luciferase Reporter Constructs
Article Snippet: The pc3DNAHIF-1α3xSDM construct was a kind gift from Dr. Christine Warnecke (University Erlangen-Nuremberg, Erlangen, Germany). pEGFPN-1 was purchased from Clonetech. .. RPMI 1640 with L-glutamine (RPMI), Dulbecco's Modified Eagle Medium (DMEM), 1× Dulbecco's Phosphate Buffered Saline (PBS), were purchased from Cellgro. .. Fetal Bovine Serum (FBS) was purchased from BioWest.

Infection:

Article Title: A Dual Infection/Competition Assay Shows a Correlation between Ex Vivo Human Immunodeficiency Virus Type 1 Fitness and Disease Progression
Article Snippet: PBMC from HIV-seronegative blood donors were obtained by Ficoll-Hypaque density gradient centrifugation of heparin-treated venous blood. .. Prior to HIV-1 infection, the cells were stimulated with 2 μg of phytohemagglutinin (PHA; Gibco BRL) per ml for 3 to 4 days and maintained in RPMI 1640–2 mM l -glutamine medium (Cellgro) supplemented with 10% fetal bovine serum (Cellgro), 10 mM HEPES buffer (Cellgro), 1 ng of interleukin-2 (IL-2; Gibco, BRL)/ml, 100 U of penicillin/ml, and 100 μg of streptomycin/ml (both from Cellgro). .. MT4 cells (obtained through the AIDS Research and Reference Reagent Program from Douglas Richman [ ]) were cultured as described above but in the absence of IL-2 and PHA.

Article Title: In Vitro Intersubtype Recombinants of Human Immunodeficiency Virus Type 1: Comparison to Recent and Circulating In Vivo Recombinant Forms
Article Snippet: PBMC from HIV-seronegative blood donors were obtained by Ficoll-Hypaque density gradient centrifugation of heparin-treated venous blood and cultured as described previously ( ). .. Prior to HIV-1 infection, cells were stimulated with 2 μg of phytohemagglutinin (PHA) (Gibco BRL) per ml for 3 to 4 days and maintained in RPMI 1640-2 mM l -glutamine medium (Cellgro) supplemented with 10% fetal bovine serum (Cellgro), 10 mM HEPES buffer (Cellgro), 1 ng of interleukin-2 (IL-2) (Gibco BRL) per ml, 100 U of penicillin (Cellgro) per ml, and 100 μg of streptomycin (Cellgro) per ml. ..

Article Title: Characterization of a Subtype D Human Immunodeficiency Virus Type 1 Isolate That Was Obtained from an Untreated Individual and That Is Highly Resistant to Nonnucleoside Reverse Transcriptase Inhibitors
Article Snippet: Peripheral blood mononuclear cells (PBMCs) from HIV-seronegative blood donors were obtained by Ficoll-Hypaque density gradient centrifugation of heparin-treated venous blood. .. Prior to HIV-1 infection, cells were stimulated with 2 μg of phytohemagglutinin (PHA) (Gibco BRL) per ml for 3 to 4 days and maintained in RPMI 1640- 2 mM l -glutamine medium (Cellgro) supplemented with 10% fetal bovine serum (Cellgro), 1 ng of interleukin-2 (IL-2) (Gibco BRL) per ml, 100 U of penicillin (Cellgro) per ml, and 100 μg of streptomycin (Cellgro) per ml. .. Human T-cell lymphotropic virus type 1-infected cell line MT2 or MT4 was cultured in RPMI 1640 medium supplemented with 10% fetal calf serum, 100 U of penicillin (Cellgro) per ml, and 100 μg of streptomycin (Cellgro) per ml.

Article Title: Impaired human immunodeficiency virus type 1 replicative fitness in atypical viremic non-progressor individuals
Article Snippet: .. Cells and viruses Peripheral blood mononuclear cells (PBMC), obtained from HIV-seronegative donors, were stimulated with 2.5 µg/ml of phytohemagglutinin (PHA; Gibco BRL) and maintained in RPMI 1640/2 mM l -glutamine media (Cellgro; Mediatech, Herndon, VA) supplemented with 10% fetal bovine serum (Cellgro), 10 mM HEPES buffer (N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid; Cellgro), 1 ng/ml of interleukin-2 (IL-2) (Gibco, BRL), 100 U of penicillin/m (Cellgro) and 100 μg of streptomycin/ml (Cellgro), for three days before infection with HIV-1 [ ]. ..

Cell Counting:

Article Title: N-BLR, a primate-specific non-coding transcript leads to colorectal cancer invasion and migration
Article Snippet: As control, we used a pool of non-targeting siRNAs (Dharmacon). .. Cell count and viability Colo320, SW620, and SW480 cells were cultured in RPMI 1640 1X with L-Glutamine medium (#10-040-CV, Corning Cellgro), whereas HCT116, RKO, and HT-29 cells were cultured in McCoy’s 5A 1X with L-Glutamine medium (#10-050-CV, Corning Cellgro), supplemented with 10% fetal bovine serum and 1% penicillin and streptomycin. .. CRC cell lines were plated in 24-well plates at a concentration of 1 × 105 cells/well in an antibiotic-free medium one day before transfection.

Enzyme-linked Immunospot:

Article Title: Interleukin-15 Increases Effector Memory CD8+ T Cells and NK Cells in Simian Immunodeficiency Virus-Infected Macaques
Article Snippet: IFN-γ ELISpot assays were performed using an IFN-γ ELISpot kit (ALP) (Mabtech, Mariemont, Ohio). .. Briefly, 96-well ELISpot IP multiscreen plates (Millipore, Billerica, Mass.) were treated with 70% ethanol, coated with 7.5 μg of capture antibody/ml in 0.05 M carbonate-bicarbonate buffer (pH 9.6) overnight at 4°C, washed five times with PBS, and blocked with RPMI 1640 (Cellgro)-10% heat-inactivated fetal bovine serum (FBS) (Serologicals Corporation, Norcross, Ga.)-2 mM l -glutamine-100 U of penicillin/ml-100 μg of streptomycin-sulfate/ml (Cellgro) (culture media) for 2 h at 37°C. .. PBMC were resuspended at a concentration of 0.5 × 106 cells/0.5 ml either in culture media plus 2.5 μg of anti-CD28 antibody (CD28.2; eBioscience)/ml and 2.5 μg of anti-CD49d antibody (9F10; eBioscience)/ml alone (nonspecific control) or in the presence of SIVmac239Gag peptide pool 1 (peptides 1 to 63, each at a concentration of 1 μg/ml), SIVmac239Gag peptide pool 2 (peptides 64 to 125, each at a concentration of 1 μg/ml) (antigen-specific stimulation), or 10 ng of phorbol myristate acetate (EMD Biosciences, San Diego, Calif.)/ml-1 μg of ionomycin (EMD Biosciences)/ml (positive control).

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  • 86
    Cellgro l glutamine
    L Glutamine, supplied by Cellgro, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l glutamine/product/Cellgro
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    l glutamine - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Cellgro 2 mmol l l glutamine
    2 Mmol L L Glutamine, supplied by Cellgro, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2 mmol l l glutamine/product/Cellgro
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    2 mmol l l glutamine - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

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